Agung Eru Wibowo
Pusat Teknologi Farmasi dan Medika - BPPT

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UJI AKTIVITAS SEDIAAN HAIR TONIC KOMBINASI EKSTRAK DAUN PARE (MOMORDICA CHARANTIA) DAN EKSTRAK WORTEL (DAUCUS CAROTA L.) PADA KELINCI JANTAN NEW ZEALAND WHITE Hendriani, Ines Nur; Tamat, Swasono R; Wibowo, Agung Eru
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan Vol 6, No 2 (2019)
Publisher : Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan

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Abstract

ABSTRACTA research about hair fertilizer test has been done by using a combination of bitter melon leaf and carrot extracts. Bitter melon leaf (Momordica charantia) is known for having hair nutritive activity, and carrot (Daucus carota L.) is also known for containing vitamin A to nourish the hair scalp. In this research, 3 months old New Zaeland white male rabbit with 2 kg of weight was tested to find out the hair growth activity of single extract and the combination of bitter melon leaf extract (EDP) and Carrot Extract (EW) with a ratio of 1EDP:1EW ; 3EDP:1EW; 3EDP:2EW. The growth and weights of hair that has been smeared on the rabbit's back were observed for 28 days. The results on combination extract with 3EDP: 1EW showed the best result with an average growth of 5.23 mm per week and a weight of 53.44 mg. The result of continued activity test of hair tonic with  concentration of 1%; 2% and 3% showed that hair tonic with 3% concentration had the best activity that is 11.56 mm for the average of hair growth and 51.16 mg for its weight. The result of physicochemical analysis and irritation showed that the color of hair tonic had brown, aromatic, clear, homogeneous, had a specific gravity of 0.9419 and had not causes irritation. Keywords : Hair Tonic Formula, Growth Activity,   Bitter Melon Leaf Extract,   Carrot Extract, Rabbit, Irritation. ABSTRAK Telah dilakukan penelitian pengujian penyubur rambut dari  kombinasi ekstrak daun pare dan wortel. Daun pare (Momordica charantia ) diketahui memiliki aktivitas sebagai penyubur rambut, dan wortel (Daucus carota L .) mengandung vitamin A untuk menutrisi kulit kepala rambut. Dalam penelitian ini dilakukan pengujian pada kelinci jantan New Zealand White berumur 3 bulan dengan bobot 2kg untuk mengetahui aktivitas penumbuh rambut ekstrak tunggal dan  kombinasi dari Ekstrak Daun Pare (EDP) dan Ekstrak Wortel (EW) dengan perbandingan 1EDP:1EW; 3EDP:1EW; 3EDP:2EW. Pertumbuhan rambut dan bobot rambut yang telah diolesi pada punggung kelinci diamati selama 28 hari. Hasil uji pada  kombinasi ekstrak, perbandingan  3EDP:1EW menunjukkan hasil terbaik dengan rata-rata pertumbuhan 5.23 mm perminggu dan bobot 53.44 mg. Hasil uji aktivitas lanjutan sediaan hair tonic dengan konsentrasi 1%; 2% dan 3%, menunjukkan bahwa sediaan hair tonic dengan konsentrasi 3% memiliki aktivitas paling baik yaitu rata-rata panjang rambut 11.56 mm rambut dan bobot rambut 51.16 mg. Hasil uji analisis fisikokimia dan uji iritasi menunjukkan bahwa sediaan hair tonic berwarna coklat, bau aromatik, jernih, homogen, memiliki berat jenis 0,9419 dan tidak menimbulkan iritasi.Kata kunci : Formula Hair Tonic,  Aktivitas Penyubur Rambut,   Daun Pare,   Wortel,  Kelinci, Iritasi
The Influence of DMBA (7,12-dimethylbenz-[a]anthracene) Regimen In The Development of Mammae Carcinogénesis on Sprague Dawley Female Rat Wibowo, Agung Eru; Sriningsih, .; Wuyung, Puspita Eka; Ranasasmita, Raafqi
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

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Abstract

There are many methods for development  of mammae cancer animal model, one of which is chemical induction using carcinogenic  agent, DMBA. This research aimed to explore the influence of dose and time regimens of DMBA on development of mammae carcinogenesis on Sprague dawley female rats. The first study was 50 rats treated with 20 mg/kg bw of DMBA orally for eleven times at twice a week. Morphological evaluations were conducted with mammae palpation for 15 weeks and then all of  rats were sacrificed for collecting mammae organs for histological analysis using hematoxylin-eosin staining. The results showed that the first and the latest nodules appeared at the fourth-week and the fourteenth-week after ending DMBA induction, respectively, in which the  most often nodule appearances were at the seventh-week. The number of nodule incidence and multiplicity were by 74% and 2 noduls/rat, respectively. Histological analysis of mammae glands determined that they fell under in Ductal Carcinoma Invasive (DCIV) category. The second study was 25 rats gavaged orally with DMBA at dose 20 mg/kg bw for five times every three days. After palpating for 15 weeks, the results showed that no nodule was observed but the histological analysis demonstrated developing of mammae gland carcinogenesis reaching about 60%  Ductal Carcinoma Insitu (DCIS) and 40% Ductal Carcinoma Invasive (DCIV) stages. Based on the results of this study can be concluded that the dose and frequency of DMBA will affect the successful development of mammary gland carcinogenesis. In DMBA induction with low frequency, no data showed the incidence and multiplicity of tumor, but histopathologic level  carcinogenesis can be distinguished. In DMBA induction with high frequency, incidence and multiplicity of tumor data can be obtained but can not be distinguished histopathologically.
QSAR Analysis of Rocaglamide Derivatives Cytotoxic Activities Using LFER Hansch Model Firdayani, .; Kusumaningrum, Susi; Setyo Utomo, Doddy Irawan; Wibowo, Agung Eru; Chaidir, .
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Rocaglamide derivatives are the compounds which have featuring cyclopenta[b]tetra-hydrobenzofuran skeleton. Until now it includes more than 50 naturally occurring derivatives. They were chosen to be interesting candidates for possible therapeutic agents primarily in the field of cancer chemotherapy due to their cytotoxic activities data against various cancer cells. A quantitative structure activity relationship (QSAR) studies were done to investigate physicochemical properties of molecule which  contribute to their activities. Series of rocaglamide derivatives have been used and analyzed using linear free energy regression Hansch model for their cytotoxic activities against MONO-MAC-6 leukemia cells, RAJI lymphoma cells and MEL-JUSO melanoma cells. Results showed that the best QSAR equations were revealed involving C Log P and CMR parameters with nonlinear regression relationships in cytotoxic activities of rocaglamide derivatives against cancer cells above.
Isolation and structure elucidation of main compound originated from sponge Axynissa aplysinoides Sumaryono, Wahono; Wibowo, Agung Eru; ., Chaidir
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 4, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Isolation of the main compound of sponge Axynissa aplysinoides collected from Lombok seawater, has been conducted. Methanol extract of dried sponge was fractionated into nonpolar, semipolar and polar fractions using hexane, ethyl acetate and buthanol. Identification and final purification of the main compound was done using column chromatography and high performance liquid chromatography (HPLC). Structure elucidation of the main compound was performed using mass spectrometry (EIMS), 1H- and 13C-NMR spectroscopies. The main compound was identified as (E)-(4-hydroxystyryl) trimethylammonium.Key words : isolation, structure elucidation, Axynissa aplysinoides
Analisis Urea-Kreatinin Tikus Putih pasca Pemberian Ekstrak Buah Mahkota Dewa dan Herba Pegagan SUMARYONO, WAHONO; WIBOWO, AGUNG ERU; NINGSIH, SRI; AGUSTINI, KURNIA; SUMARNY, ROS; AMRI, FITRIANIAR; WINARNO, HENDIG
JURNAL ILMU KEFARMASIAN INDONESIA Vol 6 No 1 (2008): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

The evaluation of toxicity of a mixed herbal extract containing mahkota dewa fruits (Phaleria macrocarpa Scheff. Boerl) and pegagan leaves (Centella asiatica L. Urban) on Wistar-strain rats had been carried out by the determination of the urea and creatinine content in urine and plasma after feeding. Oral doses of 100 mg, 500 mg, and 2500 mg of the mixed extract/kg body Weight were administered for 16 consecutive weeks to three groups of rats. Each treated group consisted of 15 males and 15 females, and the control group was represented by 10 males and 10 females. Samples of urine and plasma of the treated groups were taken at the time right before treatment (Week zero) and at Sm, 16th, 18th Week, while those of the control were taken at zero Week, 8th, and 16th Week, respectively. The result showed that theurea and creatinine contents among the treated and control groups were not signihcantly different. It could be concluded that oral administration ofthe mixed extract by a dose up to 2500 mg/kg body weight for 16 Weeks did not influence the urea and creatinine contents both in urine and plasma of the treated animals. Based on this result, it could be assumed that the use of the mixed extract is safe.
Efek Peningkat Respon Imun dari Ekstrak Etanol Pegagan (Centella Asiatica Urban) pada Tikus NINGSIH, SRI; WIBOWO, AGUNG ERU
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 2 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Penelitian ini bertujuan memferifikasi aktivitas ekstrak etanol pegagan atau Centella asiatica Urban (CA) local sebagai peningkat imunitas tubuh dengan parameter uji kapasitas dan aktivitas fagositosis makrofag peritoneum secara in vivo. Tikus jantan galur Sprague Dawley dibagi menjadi 4 kelompok, masing-masing terdiri dari 5 ekor. Kelompok dosis 1, dosis 2 dan dosis 3 diberi ekstrak 10, 20 dan 40 mg/200 g BB . secara oral selama 15 hari berturut-turut, dan kelompok kontrol mendapat pembawa. Satu hari setelah pemberian ekstrak yang terakhir, semua hewan coba dibunuh setelah diinduksi dengan bakteri Staphylococcus epidermidis (109 CFU per ekor) i.p. Aktivitas makrofag dan kapasitas makrofag peritoneum dihitung dengan dengan pewarnaan giemsa menggunakan mikroskop. Hasil menunjukkan bahwa terdapat korelasi positif antara peningkatan dosis dan aktivitas maupun kapasitas makrofag. Nilai aktivitas dan kapsitas makrofag pada kelompok yang mendapat ekstrak 40 mg/200 g BB secara statistik berbeda secara bermakna dibanding kelompok control (96% aktivitas makrofag dan 61 bakteri untuk kapasitas makrofag). Berdasarkan penelitian ini dapat ditarik kesimpulan bahwa ekstrak etanol CA berpotensi untuk dikembangkan sebagai bahan peningkat daya imunitas tubuh. Namun demikian, masih perlu dilakukan pengujian lebih lanjut untuk mengelaborasi kemampuan peningkat system imun secara lengkap dan detail.
Karakterisasi Senyawa Utama Ekstrak Daun Aglaia elliptica Blume dan Uji Sitotoksisitas pada Sel Kanker Payudara WIBOWO, AGUNG ERU; SUYATNA, FRANS; SUMARYONO, WAHONO; SIREGAR, NURYATI CHAERANI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 7 No 2 (2009): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

AIglaia elliptica Blume is one of plant species from the genus of Aglaia, belonging to the family of Meliaceae. This group of plants is known to have powerful cytotoxic effect. This research was conducted to isolate and characterize major compound(s) from ethanol extract of Aglaia elliptical leaf that can be used as marker compound(s) for extract standardization. Approximately 1000 g of Aglaia elliptica Blume leaf dry powder was extracted with ethanol using maceration method. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Six sub-fractions were obtained from the Vacuum Liquid Chromatography (VLC) process of ethyl acetate fraction. Based on the HPLC profile and separation process using column chromatography and preparative HPLC, a major compound (with the weight of 36.80 mg) was successfully isolated from sub-fraction H. Analyses on its UV absorbance pattern, mass spectra, and proton NMR identified the isolated compound as a compound with molecular weight of 300, C18H24N2O2, known as odorin. Cytotoxicity test showed that odorin has inhibition effect against MCP-7 cell line with IC5O of 153.32 ppm.
Isolasi Sel Punca Pluripoten dengan Penanda CD105+ dan SSEA3+ dari Sel Fibroblas Kulit asal Jaringan Preputium CHURIYAH, CHURIYAH; KUSUMA, INDRA; KUSUMASTUTI, SISKA A; HADI, RESTU SYAMSUL; WIBOWO, AGUNG ERU; FABIOLA, FAIZA KARA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 2 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

The development and knowledge of stem cell research are growing quickly. Stem cells are now likely to become a modern treatment as a regenerative approach that has potential treatment for various diseases that are difficult to cure, such as genetic diseases, degenerative, trauma, and malignancy. The aimed of this study was to isolate pluripotent stem cells using human dermal fibroblast cell obtained from preputium tissue by enzymatic and tissue explants methods, followed by double positive characterization for specific markers of cluster differentiation (CD) 105 and surface-stage embryonic antigen (SSEA)-3 using magnetic-based purification system (MACS). The fibroblast cells resulted from both method were cultured and expanded by variations medium and passage cells, then were characterized for double positive CD 105+ and SSEA3+. Fibroblast cell culturing in conditioned medium, lower passage and higher density has the highest percentage of CD105+ subpopulation cells compared than fibroblast cell in standard medium, higher passage and lower density. Subpopulation of both of CD 105+ and SSEA3+ cells revealed a positive response to alkaline phosphatase dye which proving a population of stem cells.
Aktivitas Antikanker Ekstrak Daun Aglaia elliptica Blume pada Tikus Betina yang Diinduksi 7,12 dimethylbenz[a] antracene WIBOWO, AGUNG ERU; SUYATNA, FRANS D.; SUMARYONO, WAHONO; SIREGAR, NURYATI CHAIRANI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 2 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Aglaia elliptica Blume adalah tanaman dari suku Meliacecae yang mengandung senyawa cyclopenta[b]tetrahydrobenzofuran dengan efek sitotoksik kuat pada berbagai jenis sel kanker. Penelitian ini bertujuan untuk mengetahui efek hambatan karsinogenesis ekstrak etanol daun Aglaia elliptica Blume pada mamae tikus betina galur Sprague Dawley (SD) yang diinduksi 7,12 dimetilbenz[a] antracene (DMBA). Penelitian diawali dengan induksi 50 ekor tikus betina galur SD menggunakan DMBA dosis 20 mg/kg BB secara oral sebanyak 11 kali. Sebanyak 25 ekor tikus betina yang tumbuh tumor mamae dibagi dalam 5 kelompok. Tiga kelompok diberi ekstrak pada dosis 50, 100 dan 200 mg/200 g BB sehari setelah muncu tumor selama 30 hari, 1 kelompok sebagai kontrol negatif dan 1 kelompok sebagai kontrol positif (diberi doksorubisin 2 μg/200 g BB). Hasil penelitian menunjukkan bahwa induksi DMBA memberikan insidensi tumor sebesar 74% dan multiplisitas sebesar 2 nodul/ekor. Hasil analisis histopatologi tumor mamae menunjukkan bahwa tingkat karsinogenesis sampai kategori Ductal Carcinoma Invasive (DCIV). Pemberian ekstrak etanol dosis 50,100 dan 200 mg/200 g BB dapat menekan pertumbuhan volume tumor berturut-turut sebesar 30%, 33.5% dan 37.4%.
The Influence of DMBA (7,12-dimethylbenz-[a]anthracene) Regimen In The Development of Mammae Carcinogénesis on Sprague Dawley Female Rat Wibowo, Agung Eru; Sriningsih, Sriningsih; Wuyung, Puspita Eka; Ranasasmita, Raafqi
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev1iss1pp60-66

Abstract

There are many methods for development of mammae cancer animal model, one of which is chemical induction using carcinogenic agent, DMBA. This research aimed to explore the influence of dose and time regimens of DMBA on development of mammae carcinogenesis on Sprague dawley female rats. The first study was 50 rats treated with 20 mg/kg bw of DMBA orally for eleven times at twice a week. Morphological evaluations were conducted with mammae palpation for 15 weeks and then all of rats were sacrificed for collecting mammae organs for histological analysis using hematoxylin-eosin staining. The results showed that the first and the latest nodules appeared at the fourth-week and the fourteenth-week after ending DMBA induction, respectively, in which the most often nodule appearances were at the seventh-week. The number of nodule incidence and multiplicity were by 74% and 2 noduls/rat, respectively. Histological analysis of mammae glands determined that they fell under in Ductal Carcinoma Invasive (DCIV) category. The second study was 25 rats gavaged orally with DMBA at dose 20 mg/kg bw for five times every three days. After palpating for 15 weeks, the results showed that no nodule was observed but the histological analysis demonstrated developing of mammae gland carcinogenesis reaching about 60% Ductal Carcinoma Insitu (DCIS) and 40% Ductal Carcinoma Invasive (DCIV) stages. Based on the results of this study can be concluded that the dose and frequency of DMBA will affect the successful development of mammary gland carcinogenesis. In DMBA induction with low frequency, no data showed the incidence and multiplicity of tumor, but histopathologic level carcinogenesis can be distinguished. In DMBA induction with high frequency, incidence and multiplicity of tumor data can be obtained but can not be distinguished histopathologically.Keywords: DMBA, doses, carcinogenesis, mammae, histopatology