Articles

KARAKTERISASI PATOGEN HAWAR DAUN BAKTERI SECARA FENOTIPIK PADA BAWANG MERAH (ALLIUM CEPA L. KELOMPOK AGGREGATUM) Asrul, Asrul; Arwiyanto, Triwidodo; Hadisutrisno, Bambang; Widada, Jaka
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 26, No 1 (2019)
Publisher : Universitas Tadulako

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Abstract

The research aimed at determining the types of pathogen associated with bacterial leaf blight diseases of shallots. The bacteria were isolated and characterized based on the  morphological, biochemical and physiological morphology of their colony and cell.  There were eight isolates of pathogenic bacteria from pure culture.  Generally, the isolates have Gram-negative characters, short rod-shaped cells, have peritrikus flagellum and mucoid, yellow or beige colonies, round, convex or flat forms, and is shiny. The isolates react positively to catalase, urease, levan, indole production, and tween 8 tests. They also can live at temperature between 20 ? 370C, pH 5 ? 7 and tolerant to NaCl content ranging from 0 ? 8.5%. The isolates react negatively to oxidases, reduce nitrates, fluorescent pigments, arginine, gelatin and starch.  Based on these characteristics, the isolates found generally have a closer resemblance to the properties of P. ananatis with a similarity coefficient of 88%  than bacteria X. axonopodis pv. allii with a similarity coefficient of 78%.  The symptoms appeared in the plant leaves were wilted (water soaked), shrinking, curving down, chlorosis, necrosis, and dieback.
ISOLASI DAN SELEKSI PSEUDOMONAD FLUORESCENS PADA RISOSFER PENYAMBUNGAN TOMAT Nurcahyanti, Suhartiningsih Dwi; Arwiyanto, Triwidodo; Indradewa, Didik; Widada, Jaka
Berkala Ilmiah Pertanian Vol 1, No 1: AGUSTUS 2013
Publisher : Berkala Ilmiah Pertanian

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Abstract

[ENGLISH] Fluorescen pseudomonad had been isolated from the rhizosphere of grafting tomato with resisten rootstock (H 7996 and EG 203 from Asian Vegetable Research Development Center). Tomato varieties Permata and Fortuna were used as scion in grafting. Fluorescen pseudomonad was isolated on King’S B medium and used phosphate buffer 0,1 M + 0,1 % pepton. About 230 isolates of P. fluorescens were isolated from tomato rhizosphere at 14 HST and about 454 isolates at 28 HST. All isolates were tested for their capability to suppress the growth Ralstonia solanacearum in vitro. All isolates inhibited the growth of R. solanacearum with an inhibition zone of 1 mm to 7 mm or more. The mechanism growth of inhibition was bacteriostatic. About Ten isolates of P. fluorescens which had large inhibition zone, were not inhibit each other and inhibition against R. solanacearum due to nutrient competition. Keywords : tomato; grafting; Fluorescens pseudomonad [INDONESIAN] Pseudomonad fluorescens diisolasi dari risosfer tomat hasil penyambungan dengan batang bawah tahan yaitu tomat H 7996 dan terung EG 203 dari Asian vegetebles Research Development Center (Taiwan). Sebagai batang atas digunakan varietas Permata dan Fortuna. Isolasi dilakukan pada media King’s B dan menggunakan buffer phospat 0,1 M + pepton 0,1 %. Sejumlah 230 isolat P. fluorescens berhasil diisolasi dari risosfer pada 14 HST dan 454 isolat pada 28 HST. Semua isolat diuji kemampuannya dalam menghambat pertumbuhan Ralstonia solanacearum secara in vitro. Semua isolat P. fluorescens mampu menghambat R. solanacearum dengan zona hambatan antara 1 mm sampai dengan lebih dari 7 mm. Semua isolat mempunyai mekanisme penghambatan bakteriostatik. Sebanyak sepuluh isolat P. fluorescens yang mempunyai daya hambat besar, tidak saling menghambat satu dengan yang lain dan penghambatan terhadap R solanacearum yang terjadi karena adanya kompetisi nutrisi. Kata kunci: Tomat; Penyambungan; Pseudomonad fluorescens  How to citate: Nurcahyanti SD, T Arwiyanto, D Indradewa, J Widada. 2013. Isolasi dan seleksi pseudomonad fluorescens pada risosfer penyambungan tomat. Berkala Ilmiah Pertanian 1(1): 15-18
SOIL BACTERIAL DIVERSITY AND PRODUCTIVITY OF COFFEE - SHADE TREE AGRO-ECOSYSTEMS Evizal, Rusdi; Tohari, .; Prijambada, Irfan Dwidja; Widada, Jaka; Widianto, Donny
Journal of Tropical Soils Vol 17, No 2: May 2012
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2012.v17i2.181-187

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Coffee productions should have environmental values such as providing high soil microbial diversity while producinghigh yield. To examine that purposes, two experimental plots were constucted at benchmark site of Conservationand Sustainable Management of Below-Ground Biodiversity (CSM-BGBD), in Sumberjaya Subdistrict, WestLampung, Indonesia, during 2007-2010. Types of coffee agro-ecosystem to be examined were Coffea canephorawith shade trees of Gliricidia sepium, Erythrina sububrams, Michelia champaca, and no shade. Two plots wereconstructed at 5-years-coffee and 15-years-coffee. Diversity of soil bacteria was determined based on DNA fingerprinting of total soil bacteria using Ribosomal Intergenic Spacer Analysis (RISA) method. The results showed that:(1) For mature coffee (15 years old), shade-grown coffee agro-ecosystems had higher soil bacterial diversity thanthose of no shade coffee agro-ecosystem, (2) Shaded coffee agro-ecosystems were able to conserve soil bacterialdiversity better than no-shade coffee agro-ecosystem. Soil organic C and total litter biomass had positive effect onsoil bacterial diversity, (3) Types of agro-ecosystem significantly affected the bean yield of 15 years coffee. Coffeeagro-ecosystems shaded by legume trees had higher yield than those of non-legume shade and no shade coffeeagro-ecosystem, (4) Shannon-Weaver indices of soil bacterial diversity together with weed biomass and N contentof coffee leaf had positive effect on coffee bean yield.[How to Cite: Evizal R, Tohari, ID Prijambada, J Widada and D Widianto. 2012. Soil Bacterial Diversity and Productivity of Coffee - Shade Tree Agro-ecosystems. J Trop Soils 17 (2): 181-187. doi: 10.5400/jts.2012.17.2.181] [Permalink/DOI: www.dx.doi.org/10.5400/jts.2012.17.2.181]
Sebaran Penyakit Hawar Daun Bakteri di Beberapa Sentra Produksi Bawang Merah di Indonesia Asrul, Asrul; Arwiyanto, Triwidodo; Hadisutrisno, Bambang; Widada, Jaka
Biota Biota Volume 18 Nomor 1 Tahun 2013
Publisher : PBI Yogyakarta

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Penelitian ini bertujuan mengetahui daerah sebaran penyakit hawar daun bakteri di beberapa sentra pertanaman bawang merah di Indonesia dan kultivar bawang merah yang dapat diinfeksi, serta mengidentifikasi patogen penyebabnya. Penentuan lokasi pengamatan dan pengambilan sampel dilakukan secara stratified purpossive random sampling. Survei dilakukan dengan cara wawancara dan pengamatan di lapangan (observasi) terhadap kultivar bawang dan gejala penyakit yang terinfeksi oleh bakteri patogen. Sampel diidentifikasi melalui pengamatan morfologi koloni, uji postulat Koch, uji reaksi hipersensitif dan pengujian sifat-sifat biokimia dan fisiologi. Hasil penelitian menunjukkan bahwa penyakit hawar daun bakteri telah tersebar secara merata di seluruh daerah pertanaman bawang merah di Indonesia, yang meliputi Kabupaten Cirebon, Tegal, Nganjuk, Bantul, dan Sigi, dengan tingkat serangan mencapai 62,5–100%. Penyakit ini menginfeksi bawang merah kultivar Bima curut, Bauji, Biru-sawah, dan Palasa. Gejala hawar daun bakteri yang dijumpai berupa water soaking, terjadi lekukan daun, pengerutan daun,  klorosis, nekrosis, mati pucuk, pertumbuhan kerdil, dan kematian. Isolat bakteri yang ditemukan mempunyai bentuk koloni bulat, cembung, berlendir, dan berwarna kuning. Ciri morfologi koloni, gejala dan karakteristik isolat bakteri mirip dengan sifat-sifat bakteri Xanthomonas axonopodis pv. allii penyebab penyakit hawar daun pada bawang bombay.Kata kunci: Sebaran, bawang merah hawar daun bakteri, Xanthomonas axonopodis pv. allii
PENGARUH INOKULASI JAMUR MIKORIZA ARBUSKULA TERHADAP GLOMALIN, PERTUMBUHAN DAN HASIL PADI Syamsiyah, Jauhari; Sunarminto, Bambang Hendro; Hanudin, Eko; Widada, Jaka
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 11, No 1 (2014)
Publisher : Faculty of Agriculture, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v11i1.214

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Penelitian pot ini bertujuan untuk mengukur kandungan glomalin,  pertumbuhan dan hasil tanaman padi dari inokulalsi mikoriza. Penelitian disusun dengan Rancangan Acak Lengkap dari empat perlakuan yaitu dua taraf sterilisasi (TO, tanpa sterilisasi dan TS, + sterilisasi) dan dua taraf inokulasi mikoriza (M1, - mikoriza dan M1+ mkorisa) dengan enam kali ulangan. Mikoriza sebanyak 5 g/pot diberikan sebelum penamanan benih padi. Hasil penelitian menunjukkan bahwa Glomalin Total (GT) dan Glomalin mudah diekstrak (GEE) lebih tinggi pada inokulasi mikoriza, masing-masing meningkat 16 % dan 20% pada tanah tidak steril (TOM1) dan  25 % dan 11 %  pada tanah steril(TSM1) dibandingkan tanpa mikoriza. Kandungan GT berkisar dari 4,95 – 9,74 mg/ g tanah dan GEE 0,99 – 2,78 mg/g tanah. Inokulasi mikoriza meningkatkan C organik tanah, sebesar 13,47 %  pada tanah tak steril dan 12,93 % pada tanah steril. Tinggi tanaman, jumlah anakan dan berat gabah kering giling (GKG) nyata dipengaruhi inokulasi mikoriza. GKG pada tanah steril+ mikoriza paling tinggi (20,68 g/pot) namun tidak berbeda nyata dengan  tanah tak steril + mikoriza. Sterilisasi tanah nyata tidak mempengaruhi produksi glomalin, pertumbuhan dan hasil tanaman padi.
DISTRIBUTION OF AMMONIUM-OXIDIZING BACTERIA IN SEDIMENT WITH RELATION TO WATER QUALITY AT THE MUSI RIVER, INDONESIA Melki, Melki; Isnansetyo, Alim; Widada, Jaka; Murwantoko, Murwantoko
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.826 KB) | DOI: 10.4308/hjb.25.4.198

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The Musi River is located in the southern Sumatra, Indonesia. Most of activities, i.e. agricultural, industrial, and urban activities are considered as being major sources of chemicals and nutrients with their waste products effluent into the river. Nitrification, the microbial oxidation of ammonia to nitrite and nitrate, occurs in a wide variety of environments and naturally remove anthropogenic N pollution. The purpose of this research was to determine of distribution of ammonium-oxidizing bacteria (AOB) in sediment with relation to water quality at the Musi river area. This study was conducted on rainy and dry season 2016 at five sampling sites from the freshwater to seawater at high and low tide conditions, the sampling sites are station St1 (Gandus), station St2 (Palembang city), station St3 (Upang), station St4 (Sungsang), and station St5 (Sea). Sediment samples were collected from the surface layer by using an Ekman grab. Some water quality such as salinity, temperature, pH, and dissolved oxygen (DO) were directly analyzed in the field, while other water quality such as NH4-N, NO2-N, and NO3-N were analyzed in the laboratory. The Density of AOB was determined by the most probable number of (MPN) method. The PCA was used to correlate variations of the AOB with physicochemical properties using software Xlstat. The results showed that the physicochemical properties had a range of salinity of 0 to 20 ppt, temperature of 29.21 to 31.82oC, pH of 4.88 to 7.93, DO of 3.44 to 11.33 mg/l, NH4-N in sediment of 0.04 to 0.87 mg/l, NO2-N in sediment of 0.01 to 1.77 mg/l, NO3-N in sediment of 0.09 to 2.08 mg/l. The density of AOB ranged from 7.2 x 102 to 6.1 x 103 cells/g sediment. Principal component analyses showed that temperature, pH, DO, and concentrations of nutrient contributed to density of AOB.
KETERSEDIAAN FOSFOR PADA TANAH ANDISOL UNTUK JAGUNG (ZEA MAYSL.) OLEH INOKULUM BAKTERI PELARUT FOSFAT Tamad, ,; Ma?as, Azwar; Radjagukguk, Bostang; Hanudin, Eko; Widada, Jaka
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 2 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.588 KB) | DOI: 10.24831/jai.v41i2.7516

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Andisols has relatively low phosphorus availability due to its adsorption by allophane. Phosphate solubilizing bacteria (PSB) increases the availability of P via release of adsorpted-P. The aims of this research were to determine: 1) anorganicP solubilization, 2) organic-P mineralization, 3) blocking of Andisols adsorption site, and 4) effective PSB inoculant. The research was arranged in completely randomized design, with PSB inoculant as treatment. Variables observed were solubleP, mineralize-P, adsorpted-P, pH, total acidity, PSB population, phosphatase and phythase activity, relative surface charge, and maize?s growth component. The result showed that PSB inoculation increased soluble-P from 30 to between 150 and 195 ppm P, increased mineralize-P from 23.7 to between 63.6 and 91.7 ppm P, and decreased P-adsorption from 95 to between 36 and 13%. PSB inoculation decreased the Andisols pH, increased the total acidity, PSB population, the phosphatase and phytase activity, and PSB had relatively high of relative surface charge (69%). The PSB inoculation increased maize P absorption in the range of 70 and 75 mg P plant-1, and increased relative agronomic effectiveness (RAE )between 145 and 150%. Liquid and solid PSB inoculant had no different effect in increasing maize growth. Keywords: Andisol, P release, phosphate solubilizing bacteria, phosphatase, phytase
Succession of Actinomycetes During Composting Proccess of Dairy-Farm Waste Investigated by Culture-Dependent and Independent Approaches Faatih1, Mukhlissul; Widada, Jaka; N, Ngadiman
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Mesophilic, thermophilic, and maturation phases were recognized in composting proccess. Temperaturechanges influence the microbial communities in compost within composting proccess. Actinomycetes account for alarger part of compost microbial population. The aim of this research was to study succession of actinomycetescommunity during composting of dairy-farm waste investigated by culture-dependent and independentapproaches.In culture-independent method, the succession of actinomycetes community was analyzed by nestedpolymerasechain reaction of ribosomal intergenic spacer (nested-PCR RISA) using spesific primer F243 and primerR23S followed by a second PCR using primers F968 and R23S. In culture-dependent method actinomycetes fromcompost were isolated on selective media, starch-nitrate medium and humic-acid + vitamins medium. DNA ofactinomycetes was extracted and amplified by repetitive sequence-based PCR (rep-PCR) using primer BOXA1R. Thebanding patterns were used to generate dendrograms by UPGMA clustering with NTSYS program. Microcosmcontaining sterile rice-straw and water which is inoculated with each actinomycetes isolates was used for examiningthe ability of each isolate in rice-straw degradation.The experiment results showed that succession of both bacteria and actinomycetes was occured withincomposting proccess of dairy-farm waste. Analysed by culture-independent method revealed that the highestcommunity of compost’s bacteria was on mesophilic, thermophilic, and maturation phases, respectively. WhereasPCR-nested RISA resulted the highest population of actinomycetes was on thermophilic, maturation, and mesophilicphases, respectively. By culture-dependent method was obtained 29 actinomycetes isolates from mesophilic phase,23 isolates from thermophilic phase, and 19 isolates from maturation phase. Genetic diversity analysis of the obtainedisolates showed the presence of phylogenetic grouping on each phase of composting proccess. This result illustratedthe occurance of succession of actinomycetes community in compost. The ability of each isolates in rice-strawdegradation was different, and SnT9 isolate was found to be a promising rice-straw degrader.Keywords: succession, actinomycetes, composting, nested-PCR RISA, rep-PCR
Diversity of Actinomycetes at Several Forest Types in Wanagama I Yogyakarta and Their Potency as a Producer of Antifungal Compound Nurjasmi, Reni; Widada, Jaka; N, Ngadiman
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

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Actinomycetes are bacterial groups that produce many secondary metabolites, which different biological activities, such as antifungi, antibacteria, antivirus, antitumor, etc. Actinomycetes are widely distributed in soil and their diversity is influenced by type of forest. The aim of this study is to investigate diversity of actinomycetes in several forest types of Wanagama I forest in Yogyakarta and their potency as a producer of antifungal compound. Soil samples under the forest of Tectona grandis, Swietenia macrophylla King, Bamboosa vulgaris, Melaleuca leucadendron, and Gliricidia maculata were used as sources of soil bacteria. Bacteria and actinomycetes communities were analyzed through culture-independent approach by RISA and nested-PCR RISA using actinomycetes spesific primer (F243), respectively. Through culture-dependent approach, isolated actinomycetes diversity were analyzed by identification of morphology (colony and cell), genetic (BOX element by rep-PCR), and secondary metabolites (thin layer chromatography). In addition, isolates were assayed for their antifungal activity against Saccharomyces cerevisae, Candida albicans, Fusarium oxysporum and Aspergillus flavus. The presence of Polyketide Synthase-I (PKS-I) and NonRibosomal Peptide Synthetase (NRPS) genes were amplified by PCR to study their correlation with antifungal activity of the actinomycete isolates. The results showed that types of forest influence diversity of rhizobacteria especially actinomycetes. According to culture-independent approach, relatively, com-</div><div>munity of rhizobacteria from the highest were soil under the forest of B. vulgaris, G. maculata, T. grandis, S.macrophylla King, and M. leucadendron, respectively. Meanwhile, community of actinomycetes from the highest were soil under the forest of G. maculata, B. vulgaris, M. leucadendron, S. macrophylla King, and T. grandis, respec- tively. Fourty-three morphologically different isolates were found by using culture-dependent approach consisting of 17 isolates were found in soil under the forest of M. leucadedron, each of 9 isolates in G. maculata and T. grandis, 6 isolates in S. macrophylla King. and 2 isolates in B. vulgaris. More diversity of secondary metabolites were observed in soil actinomycetes under the forest of M. leucadendron. Of the 43 isolates, 100% were active against S.cerevisae, 37.20% against C. albicans, 95.30% against F. oxysporum, and 83.70% against A. flavus. Antifungal activity of actinomycete isolates did not always have correlation with the presence of PKS-I and NRPS.
Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate M, Murwantoko; Widada, Jaka; Nuraini, Yani Lestari
Indonesian Journal of Biotechnology Vol 13, No 1 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during active penetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targeted cell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasite successfully enter the cell target then Granule (GRA) proteins are responsible for the formation of parasitophorus vacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently, this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone and sequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique. Total ribonucleic acid (RNA) was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA was used as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor from Riboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinant plasmid was transformed into E. coli (XL1-Blue). The transformed E. coli XL-1 Blue were plated on LB agar containing X-Gal, IPTG and ampicillin. Recombinant clones (white colony) were picked up and grown up in the LB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order to identify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolated using alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid was cut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward and M13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretory and secretory protein which has molecular weight of 54 kDa. The DNA alignments of sequence from the cloned gene showed 97% homology with gene encoding for ROP-2 of T. gondii RH isolate., string),(99, en_US, subject, Toxoplasma gondii, tachizoite, ESA, complementary DNA, ROP2
Co-Authors , Tamad . Tohari Afi Tarmiawati ALIM ISNANSETYO Alimuddin . Alimuddin A, Alimuddin Alimuddin, A. Amekan, Yumechris Anna Safarrida, Anna Ariani Hatmanti Arifah Khusnuryani, Arifah Asrul Asrul Azwar Maas Bambang HADISUTRISNO Bambang Hariwiyanto Bambang Hendro Sunarminto Bostang Radjagukguk Camelia Herdini Christanti Sumardiyono Dewi Seswita Zilda Dhani Suryawan, Dhani Didik Indradewa Dilin Rahayu Nataningtyas Dinar Mindrati Fardhani, Dinar Mindrati Dionysius Andang Arif Wibawa, Dionysius Andang Dolly Iriani Damarjaya, Dolly Iriani Donny Widianto Duranta D. Kembaren, Duranta D. Edy Meiyanto Eko Hanudin Endang Semiarti Endang Sutriswati Rahayu Eni Harmayani Erni Martani Gintung Patantis H. Hartono, H. Hari Eko Irianto Hartono H, Hartono Hosoyama, Akira Irfan D. Prijambada IRFAN DWIDYA PRIJAMBADA Istini Istini Jauhari Syamsiyah Keishi Senoo, Keishi Lucia Dhiantika Witasari, Lucia Dhiantika Ma?as, Azwar Masaya Nishiyama, Masaya Melki Melki Mentari, Diani Muhammad Nur Cahyanto Mujiyo Mujiyo Mukhlissul Faatih Mukhlissul Faatih1, Mukhlissul Mulyadi Mulyadi Murwantoko M, Murwantoko Murwantoko Murwantoko Murwantoko, M. Mustofa . Mustofa M, Mustofa Mustofa, M. Naima, Mirtani Nastiti Wijayanti Ngadiman ., Ngadiman Ngadiman N, Ngadiman Ngadiman, N. Nojiri, Hideaki Nur Edy Nur Prihatiningsih Nuringtyas, Tri Rini Ocky Karna Rajasa, Ocky Karna Pintaka Kusumaningtyas, Pintaka Prahastiwi, Yuliana Prijambada, Irfan Dwidja PUJI LESTARI PUSPITA LISDIYANTI Putu Sudira Reni Nurjasmi, Reni Ristiarini, Susana Riyanti . Riyanti, R. RUSDI EVIZAL Sarto Sarto Shigeto Otsuka, Shigeto Shinta Hartanto, Shinta SITI KABIRUN Siti Subandiyah Sofia Mubarika Sri Nuryani Hidayah Utami, Sri Nuryani Hidayah Sri Suryanti Sri Wedhastri Stalis Norma Ethica Subagus Wahyuono Sudadi Sudadi Suhartiningsih Dwi Nurcahyanti Sumarno Sumarno Supardan, Dadan Suryanti Suryanti Susila Herlambang Tamad, Tamad Tohari Tohari Tri Joko Raharjo Tri Wibawa Triwidodo ARWIYANTO Triyanto Triyanto Wangi, Dyah Sekar A P Widya Asmara Wulansari, Riska Yamazoe, Atsushi Yani Lestari Nuraini, Yani Lestari Yenny Sariasih Yuliana Yuliana Prahastiwi, Yuliana Yusro Nuri Fawzya Yuyun Farida, Yuyun Zilda, Dewi Zeswita