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APPLICATION OF CYTOKININ AND MYCORRHIZA TO INCREASE PRODUCTION AND QUALITY OF PINEAPPLE SEEDLINGS FROM CROWN LEAF BUD CUTTINGS Putri, Dirgahani; Suhartanto, M. Rahmad; Widajati, Eny
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

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Abstract

The pineapple vegetative propagation method using crown leaf bud cuttings has potential to produce seedlings of greater uniformity and in larger quantities than other methods. This study, aimed to explore the potential eff ects of concentration and frequency of Benzylaminopurine (BAP) application for increasing the production and quality of pineapple seedlings from crown leaf bud cutting propagation. The experiment used a randomized complete block design (RCBD) with two factors. The fi rst factor was the concentration of BAP (0, 200, 400, 600, 800 ppm) and the second factor was the frequency of BAP application (1, 2, 3 times). Furthermore, this study investigated the eff ects of applying mycorrhizae to accelerate seedling growth and improve seedling quality during production from crown leaf bud cutting propagation. The experiment used a RCBD with one factor (dose of mycorrhiza: 0, 50, 100, 150, 200 spores). The results indicated that the eff ects of concentration and frequency of BAP application and their interaction increased seed production as determined by sprout cuttings at 5 weeks after planting by applying 600 ppm 2 times. Generally, with increasing concentration and frequency of BAP application, leaf chlorophyll content was reduced. However, the application mycorrhiza positively infl uenced the production of seedlings from pineapple crown bud cuttings and improved seedling quality as measured by root dry weight, chlorophyll content, root infection, and phosphatase enzyme activity.
PERLAKUAN COATING DENGAN MENGGUNAKAN ISOLAT METHYLOBACTERIUM SPP. DAN TEPUNG CURCUMA UNTUK MENINGKATKAN DAYA SIMPAN BENIH PADI HIBRIDA Widajati, Eny; Salma, Selly; Lastiandika, Yuyuk Agung
Buletin Agrohorti Vol. 1 No. 1 (2013): Januari 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.532 KB) | DOI: 10.29244/agrob.1.1.79-88

Abstract

This research aims to study the effect of coating on seed storability in three varieties of hybrid rice. The research conducted at Centre for Soil Research, East West Corporation in Purwakarta and Laboratory of Seed Science of Department Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University.This experiment arranged in nested plot design in which formulation of seed coating (uncoated seed, TDJ-7, TD-TPB3, TD-TM1 and Curcuma flour) was nested in six times period of storage. The result showed that coated and uncoated seed still had high viability after storage 15 weeks for 3 varieties of hybrid rice seeds (DG-1 SHS, SL-8 SHS and INTNANI-2). Methylobacteriumspp. strain TD-TPB3 and TD-TM1 showed to be the best coated for hybrid rice seeds.Keyword :Curcuma, Methylobacteriumspp., storage, viability.
PERLAKUAN BENIH DIANTARA PERIODE PENYIMPANAN UNTUK MENINGKATKAN DAYA SIMPAN BENIH KEDELAI (GLYCINE MAX (L.) MERR.) Kusumastuti, Shinta Nugraheni; Widajati, Eny
Buletin Agrohorti Vol. 5 No. 2 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.602 KB) | DOI: 10.29244/agrob.5.2.242-250

Abstract

Tujuan penelitian ini adalah mendapatkan perlakuan benih diantara periode penyimpanan  untuk meningkatkan daya simpan benih kedelai. Penelitian dilaksanakan di Laboratorium Teknologi Benih, Departemen Agronomi dan Hortikultura, Fakutas Pertanian, Institut Pertanian Bogor pada bulan Desember 2013 hingga April 2014. Perlakuan benih  yaitu  (1) pencucian dengan air hangat , (2) pencucian dengan air dingin, (3) pencucian dengan fungisida mankozeb, (4) penjemuran di bawah matahari. Benih yang telah diberi perlakuan (1), (2) dan (3) dikeringkan hingga mencapai kadar air 8 - 9% dengan cara dijemur di bawah matahari. Benih   disimpan di ruang suhu rendah (± 5 °C) dan di ruang kamar (26-30 °C) menggunakan plastik polipropilen (0.08 mm). Hasil penelitian menunjukkan bahwa benih yang tidak diberi perlakuan hanya mampu mempertahankan daya berkecambah 80% hingga 6 minggu. Perlakuan pencucian benih dengan fungisida mankozeb, pencucian dengan air dingin dan penjemuran benih tanpa dicuci terlebih dahulu dapat meningkatkan viabilitas benih serta dapat mempertahankan daya berkecambah >80%  hingga akhir penyimpanan, 16 minggu setelah perlakuan baik pada penyimpanan suhu rendah maupun suhu kamar. Perlakuan penjemuran di bawah sinar matahari dapat menjadi pilihan terbaik sebagai perlakuan invigorasi benih diantara periode penyimpanan karena murah dan mudah dilakukan serta memberikan hasil yang baik.
PEMANFAATAN BAKTERI PSEUDOMONAS FLOURESCENS RH4003 DAN ASAM ASKORBAT UNTUK MEMPERTAHANKAN VIABILITAS BENIH PADI HIBRIDA Krisnandika, A. A. Keswari; Widajati, Eny; Nawangsih, Abdjad Asih
Buletin Agrohorti Vol. 5 No. 2 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.619 KB) | DOI: 10.29244/agrob.5.2.205-212

Abstract

Padi hibrida memiliki glume terbuka yang berperan penting pada laju perlambatan viabilitas benih selama penyimpanan. Serta untuk mencapai hasil panen padi hibrida yang optimal membutuhkan asupan nutrisi yang berkualitas tinggi. Penelitian ini bertujuan untuk mempelajari pengaruh formula lapisan benih dengan penambahan bakteri Pseudomonas fluorescens RH4003 sebagai Pertumbuhan Tanaman Memnghasilkan Rhizobakteri dan asam askorbat sebagai antioksidan, menghambat viabilitas benih padi hibrida dalam penyimpanan. Penelitian ini terdiri dari tiga seri percobaan, yaitu menggunakan 3 varietas padi hibrida (DG-1, Intani dan SL-8), Rancangan percobaan yang digunakan yaitu petak bersarang dengan enam kali periode penyimpanan sebagai petak utama. Hasil penelitian menunjukkan bahwa lapisan yang menggunakan asam askorbat merupakan perlakuan terbaik dari semua variabel viabilitas hampir pada semua varietas. Perlakuan dengan menggunakan bakteri memiliki hasil yang signifikan (p < 0.01) meningkatkan laju pertumbuhan dan indeks vigor pada minggu keenam penyimpanan sebesar 22.48% per etmal dan 83.33% untuk varietas benih padi SL-8. Lapisan DG-1 yang menggunakan asam askorbat pada minggu keenam mengakibatkan indeks vigor tertinggi (90 %) yang signifikan (p<0.5) dan peningkatan yang sangat signifikan pada perkecambahan benih dan indeks vigor minggu kesembilan penyimpanan untuk varietas SL-8 SHS (92.67% dan 73.33%).
PENGGUNAAAN ZEOLIT UNTUK MEMPERTAHANKAN VIABILITAS BENIH PALA (MYRISTICA FRAGRANS HOUTT) SELAMA DI PENYIMPANAN Tanjung, Maisaroh; Widajati, Eny; Suwarno, Faiza C.
Buletin Agrohorti Vol. 4 No. 2 (2016): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.453 KB) | DOI: 10.29244/agrob.4.2.202-209

Abstract

Benih pala termasuk kelompok benih rekalsitran yang membutuhkan kondisi lembab selama penyimpanan. Zeolit adalah endapan vulkanik yang dimanfaatkan sebagai bahan pelembab. Kondisi lingkungan periode penyimpanan merupakan faktor penting yang mempengaruhi viabilitas benih. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh kondisi penyimpanan dengan penambahan zeolit dan periode simpan terhadap viabilitas benih pala. Percobaan disusun dalam Split Plot dengan Rancangan Acak Lengkap menggunakan tiga ulangan. Petak utama adalah kondisi simpan yaitu H0= kondisi kering (tanpa zeolit) dan H1= kondisi lembab (dengan zeolit). Anak petak adalah periode simpan (P) terdiri dari enam taraf : P0= tanpa penyimpanan (0 hari), P1  = penyimpanan 3 hari, P2  = penyimpanan 6 hari, P3  = penyimpanan 9 hari, P4  = penyimpanan 12 hari,  P5 = penyimpanan 15 hari. Hasil penelitian menunjukkan bahwa kondisi simpan dengan zeolit lebih baik dibandingkan dengan kondisi simpan tanpa zeolit dalam mempertahankan viabilitas benih pala. Kondisi simpan dan periode simpan terbukti berpengaruh nyata terhadap parameter viabilitas potensial dengan tolok ukur daya berkecambah, viabilitas total dengan tolok ukur potensi tumbuh maksimum, serta vigor benih dengan tolok ukur kecepatan tumbuh dan indeks vigor. Penyimpanan dengan zeolit meningkatkan kadar air benih dari 34.61% menjadi 39.69%, sedangkan penyimpanan tanpa zeolit mempertahankan kadar air benih dari 27.83% menjadi 22.86%. Kondisi kadar air yang tinggi dapat mempertahankan viabilitas tetap tinggi yang ditunjukkan pada viabilitas benih tetap tinggi hingga periode simpan hari-15.
APLIKASI BAKTERI PROBIOTIK UNTUK MENINGKATKAN MUTU FISIOLOGI DAN KESEHATAN BIBIT CABAI (CAPSICUM ANNUUM L.) Tefa, Anna; Widajati, Eny; Syukur, Muhamad; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.829 KB) | DOI: 10.24831/jai.v44i2.13487

Abstract

ABSTRACTThe use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period.Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period
KARAKTERISTIK MORFOLOGI BENIH SEBAGAI PARAMETER UNTUK PENENTUAN POHON INDUK SUMBER BENIH PALA (MYRISTICA FRAGRANS HOUTT.) Pramudita, Listya; Widajati, Eny; Suwarno, Faiza Chairani; Surahman, dan Memen
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.862 KB) | DOI: 10.24831/jai.v45i1.13755

Abstract

Nutmeg is an Indonesian native plant, produces essential oill as an export commodity. However, it has low seed uniformity that mengurangi keseragaman tanaman di lapang. The main objective of this research was to obtain morphological characteritics as parameter for nutmeg mother plants selection. This field research was carried out from August-December 2014 at Toisapu, Wakal and Lula nutmeg seed source plantations in Ambon, and Seed Storage and Testing Laboratory, IPB. Characterization was undertaken on ten mother plants from each  Wakal and Lula seed source plantations and eight mother plants from Toisapu seed source plantation. Quantitative and qualitative characters of vegetative, fruit, mace and seed from every mother plants were analyzed with  Gower?s similarity test. Mother plants that produced high uniformity seed was the 7th (0.80), the 8th (0.93) and the 10th (0.80) mother plants in Wakal; the 2nd (0.80) mother plant in Toisapu; the  2nd (0.82), the 5th (0.80), the 6th (0.82), the 8th (0.84), the 9th (0.80) and the 10th (0.93)  mother plants in Lula. Those selected mother plants produced morphological uniform seed morphologically. Lula seed plantation had the highest uniformity level among mother plants (0.77-0.87) compared to Toisapu (0.70-0.87) and Wakal (0.69-0.84) mother plants. The morphological characteristic can be use to select the source seed mother plants which able to produce high morphological uniformity seeds.Keywords: Gower?s similarity, seed production, uniformity
APLIKASI ENZIM LIGNINASE DAN SELULASE UNTUK MENINGKATKAN PERKECAMBAHAN BENIH KELAPA SAWIT (ELAEIS GUINEENSIS JACQ.) DI PUSAT PENELITIAN KELAPA SAWIT, PEMATANG SIANTAR, SUMATERA UTARA Hadi, Putra K; Widajati, Eny; Salma, Selly
Buletin Agrohorti Vol. 5 No. 1 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.273 KB) | DOI: 10.29244/agrob.5.1.69-76

Abstract

Penelitian ini dilakukan di Pusat Penelitian Kelapa Sawit, Pematang Siantar, Sumatera Utara, mulai dari 1 Maret hingga 24 Juli 2010. Fokus kegiatan ada pada  divisi pematahan dormansi dan perkecambahan benih di PPKS. Kesimpulan dari kegiatan ini adalah: 1) Dua varietas benih kelapa sawit diuji responnya terhadap perbedaan perlakuan yang dilakukan .; 2) Benih varietas Yangambi, ada tiga teknik aplikasi enzim ligninase dan selulase yang memberikan hasil efektif dengan peningkatan nyata dari kekuatan tumbuh, potensi tumbuh maksimum, dan kecepatan tumbuh benih serta menurunkan indeks dormansi benih. Ketiga tekni tersebut adalah P1 dengan pemanasan selama 40 hari, perendaman air (3 hari), perendaman enzim ligninase (1 hari) dan perendaman enzim selulase (2 hari); P2 dengan pemanasan selama 40 hari, perendaman enzim ligninase (1 hari), perendaman enzim selulase (2 hari) dan perendaman air (3 hari), dan P3 dengan pemanasan selama 40 hari, perendaman campuran enzim ligninase dan selulase (3 hari) dan perendaman air (3 hari).; 3) Pada varietas Langkat, ligninase dan selulase tidak memberikan peningkatan signifikan pada kekuatan tumbuh benih, potensi tumbuh maksimum, dan menurunkan indeks dormansi benih. Pada kecepatan tumbuh benih, aplikasi enzim hanya efektif di P2.; 4) Aplikasi dari ligninase dan selulase enzim dalam varietas Langkat dan Yangambi terbukti dapat mengurangi panjang pemanasan biji di ruang pemanas selama 10 hari di P4 dan selama 20 hari perlakuan di P1, P2 dan P3.; 5) Aplikasi enzim lignoselulase pada bibit kelapa sawit tidak menyebabkan gejala kelainan pada bibit.
PERUBAHAN FISIK, FISIOLOGI DAN BIOKIMIA SELAMA PEMASAKAN BENIH DAN STUDI REKALSITRANSI BENIH KEMIRI SUNAN Tresniawati, Cici; Murniati, Endang; Widajati, Eny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.208 KB) | DOI: 10.24831/jai.v42i1.8157

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ABSTRACTThe aims of this research on Reutealis trisperma seed were to know 1) seed harvest maturity level based on physiological and biochemical changes; and 2) seed recalcitrancy based on critical moisture content and storability in two storage temperatures. The research was conducted at Leuwikopo Seed Science and Technology Laboratory, from February 2012 to January 2013. There were three experiments, i.e. I. Effect of seed maturity level on seed viability and vigor (randomized completely block design, 3 seed maturity level, 3 replications); II. Determination of seed critical moisture content, used two methods, i.e. 1) fan drying and 2) air drying (completely randomized design in each drying methods, 3 replications); III. Effect of temperature and period of storage on seed viability and vigor (nested design, first factor was 2 storage temperature and the second factor was 7 storage periods, 3 replications). The result showed that seed physiological maturity achieved in 28 weeks after flowering with morphological criteria were brownish fruit, soft fruit exocarp, brown seed testa; physiological criteria was germination percentage 76-80% and biochemical criteria was carotenoid content 0.62 µmol(g FW)-1. Carotenoid content can be used as biochemical indicator to determine fruit maturity and significantly correlated with moisture content, seed dry weight and germination percentage. Critical moisture content were 8.2-10.9%. These data suggested that seeds could be classified as intermediate. R. trisperma  seeds could be stored for three weeks in temperature 19-28 oC and RH 50-70%, with 12% moisture content used polypropilen plastic bag Keywords: carotenoid, critical moisture content, desiccation, Reutealis trisperma
METODE DETERIORASI TERKONTROL UNTUK PENDUGAAN DAYA SIMPAN BENIH KEDELAI ., Nizaruddin; Suwarno, Faiza C.; Widajati, Eny; Qadir, Abdul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.291 KB) | DOI: 10.24831/jai.v42i1.8145

Abstract

ABSTRACTPhysiological quality of soybean seeds was easily deteriorated during storage. The objectives of the research were to determine controlled deterioration test which could be used to evaluate soybean seed viability and to find the appropriate controlled deterioration test for estimating storability of soybean seed. The research was conducted at the quality control laboratory PT. BISI International, Tbk., Kediri since October 2012 until February 2013. The completely randomized design with four replications was used in the experiment by moisture content and deterioration periods as treatment. The results showed that the controlled deterioration periods to evaluate two varieties of soybean seed were same for 16 hours at 17.5% moisture content for Wilis and 15% moisture content for Detam-1. The result also showed that there were significant correlations between controlled deterioration values with actual values from 16 weeks storage periods, indicating controlled deterioration test periods could estimate storage periods. Verification of seed vigor relation to storability model showed there were no significant differences between germination and speed of germination between controlled deterioration and actual values. Simulation of soybean seed storability model with germination percentage after controlled deterioration as input variables could predict the storage periods.Keywords: Glycine max, rapid ageing, seed modelling, seed vigour, vigour index