Widanarni Widanarni
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Jln. Agatis, Kampus IPB Dramaga Bogor 16680 Indonesia

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MICROENCAPSULATION OF PROBIOTICS AND ITS APPLICATIONS WITH PREBIOTIC IN PACIFIC WHITE SHRIMP LARVAE THROUGH ARTEMIA SP. Ramadhani, Dian Eka; Widanarni, Widanarni; Sukenda, Sukenda
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3603.271 KB) | DOI: 10.19027/jai.18.2.130-140

Abstract

ABSTRACTThis study aimed to produce microencapsulated probiotic Pseudoalteromonas piscicida (1Ub) and evaluate it with preb­iotic mannan-oligosaccharide (MOS) through the enrichment of Artemia sp., on bacterial population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Microencapsulation of probiotic was done by the freeze-drying method. The shrimp larvae were reared for 13 days and fed by the Artemia sp. enriched with microcapsule of probiotic 1Ub (10 g/L), prebiotic MOS (12 mg/L), synbiotic, and control without administration of microencapsulated probiotic and prebiotic, including negative (C-) and positive (C+) control. On the day 14, all of the experimental shrimp larvae except C- were challenged through immersion method with Vibrio harveyi MR5339 (107 CFU/mL). This study showed that the administration of microcapsule of probiotic 1Ub, prebiotic MOS, and synbiotic through the enrichment of Artemia sp. could increase the bacteria population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Moreover, synbiotic treatment demonstrated the best result compared to other treatments.Keywords: probiotic, prebiotic, synbiotic, Pacific white shrimp, microencapsulation ABSTRAKPenelitian ini bertujuan untuk membuat mikrokapsul probiotik Pseudoalteromonas piscicida (1Ub) dan mengevaluasinya dengan prebiotik mannan-oligosaccharides (MOS) melalui pengayaan Artemia sp. terhadap populasi bakteri, performa pertumbuhan, respons imun dan resistensi penyakit pada larva udang vaname. Mikroenkapsulasi probiotik dilakukan dengan metode freeze-drying. Larva udang dipelihara selama 13 hari dan diberi pakan Artemia sp. yang telah diperkaya dengan mikrokapsul probiotik 1Ub (10 g/L), prebiotik MOS (12 mg/L), sinbiotik, dan kontrol tanpa penambahan mikrokapsul probiotik dan prebiotik, termasuk kontrol negatif (C-) dan positif (C+). Pada hari ke-14, seluruh larva udang percobaan kecuali C- diuji tantang melalui metode perendaman dengan Vibrio harveyi MR5339 (107 CFU/mL). Hasil penelitian menunjukkan bahwa pemberian mikrokapsul probiotik 1Ub, prebiotik MOS, dan sinbiotik melalui pengayaan Artemia sp. dapat meningkatkan populasi bakteri, performa pertumbuhan, respons imun, dan resistensi penyakit pada larva udang vaname. Selain itu, perlakuan sinbiotik menunjukkan hasil terbaik dibandingkan perlakukan lainnya.Kata kunci : probiotik, prebiotik, sinbiotik, udang vaname, mikroenkapsulasi
STUDI BACILLUS FIRMUS SEBAGAI KANDIDAT PROBIOTIK DALAM MENGHADAPI AEROMONAS HYDROPHILA PADA MEDIA BUDIDAYA THE STUDY OF BACILLUS FIRMUS AS PROBIOTIC CANDIDATE IN SUPRESSING AEROMONAS HYDROPHILA IN CULTURE MEDIA Haditomo, Alfabetian Harjuno Condro; Lusiastuti, Angela Mariana; Widanarni, Widanarni
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 11, No 2 (2016): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.729 KB) | DOI: 10.14710/ijfst.11.2.111-114

Abstract

 Pengendalian penyakit bakterial yang umum dilakukan dengan pemakaian antibiotik atau  bahan kimia sudah tidak diperbolehkan lagi karena menimbulkan patogen yang resisten  terhadap bahan kimia tersebut, terlebih jika penggunaan tidak sesuai dengan anjuran yang diberikan. Dampak negatif terhadap kesehatan konsumen berupa residu antibiotik juga menjadi pertimbangan yang harus diperhatikan. Manipulasi terhadap populasi mikroba yang berada di perairan guna pencegahan sebelum terjadinya serangan bakteri yang bersifat mematikan perlu dilakukan sebagaimana konsep probiotik sebagai biokontrol. Tujuan penelitian ini adalah menguji kandidat probiotik dalam menekan atau menghambat bakteri patogen Aeromonas hydrophila. Penelitian ini dilaksananakan dalam dua tahap. Tahap pertama adalah tahap pengujian bakteri kandidat probiotik secara in vitro menggunakan metode zona hambat dan kultur bersama pada media agar.  Tahap kedua adalah uji tentang bakteri kandidat probiotik dengan patogen pada media budidaya. Hasil terbaik penelitian tahap pertama pada  uji kultur bersama antara kandidat probiotik B. firmus dengan A. hydrophila pada skala in vitro adalah dengan penambahan probiotik  B. firmus sebanyak 108 cfu/ml. Sedangkan pada penelitian tahap kedua didapatkan hasil berturut-turut perlakuan D dengan tingkat kelangsungan hidup (SR) mencapai 90%, perlakuan C dengan SR 75%, perlakuan A dengan SR 50% dan perlakuan K dengan SR 50%.  Controlling bacterial disease with the use of antibiotics or chemicals is no longer allowed as it results in pathogens that are resistant to the chemicals, especially when not in accordance with the recommendations provided. The negative impactsof the antibiotics residues on the consumers? health  also need to be considered. Manipulation of microbial populations present in the waters as preventation before the lethal attack of bacteria needs to be done which is in accordance with the concept of probiotics as biocontrol.The purpose of this study was to test the probiotic candidates in suppressing or inhibiting pathogenic bacteria Aeromonas hydrophila. This study was conducted in two stages. The first stage was to test a candidate probiotic bacteria in vitro using culture methods and inhibition zone on the media together. The second stage wasto test candidate probiotic bacteria to pathogens on the cultivation media. The best results in the first phase of the research is shared culture test between probiotic candidate B. FIRMUS with A. hydrophila on vitro scale is the addition of the probiotic B. FIRMUS 108 cfu / ml. While in the second phase of the research results obtained successively: treatment D with a survival rate (SR) reaches 90%, treatment C with SR 75%, treatment A with SR 50% and treatment K with SR 50%. 
BAKTERI PROBIOTIK DALAM BUDIDAYA UDANG: SELEKSI, MEKANISME AKSI, KARAKTERISASI, DAN APLIKASINYA SEBAGAI AGEN BIOKONTROL Widanarni, Widanarni; Sukenda, Sukenda; Setiawati, Mia
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 2 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Bacterial disease attack occurs at the hatchery stage, which is considered to be the most serious threat, and often results in mass mortality of shrimp larvae by vibrosis which is that caused by a luminous bacterium identified as Vibrio harveyi. This research was carried out to obtain local isolates of probiotic bacteria that were able to inhibit the growth of V. harveyi and effectively apply it as a biocontrol of vibriosis in shrimp cultures. The research was carried out as follows: (1) In vitro and in vivo selection of probiotic bacteria candidates, (2) Study of the action mechanism and characterization of the selected pro biotic bacteria, (3) Study on application of the selected probiotic bacteria as a biocontrol agent in shrimp cultures. Results of in vitro and in vivo selection provided the best three isolates, which were 1Ub, SKT-b and Ua. The survival rate of shrimp larvae which were not only inoculated by V. harveyi but also with 1Ub, SKT-b and Ua probiotic bacteria were 88.33, 83.33, and 81.67% respectively; where as the positive control treatment (merely inoculated with V. harveyi) gave a 41.67% survival rate and the negative control (without bacterial addition) was 68.33%. Studies using a rifampicin resistant marker (RfR) demonstrated that the number of V. harveyi MR5339 RfR cells in treatments without probiotic addition were higher than the treatment with the probiotic bacteria, in dead larvae, living larvae, as well as in the culture media. Partial sequencing of the I6S-rRNA gene showed that the I Ub isolate was similar to Pseudoalteromonas piscicida, whereas the SKT -b and Ua isolates were similar to Vibrio alginolyticus. Selected probiotic bacteria could be applied directly to shrimp larva culture media, or orally through enrichment of both natural and artificial food. Keywords: Penaeus monodon larvae, probiotic bacteria, vibriosis 
APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (LITOPENAEUS VANNAMEI) (SYNBIOTIC APPLICATION FOR PREVENTION OF INFECTIOUS MYONECROSIS VIRUS INFECTION IN WHITE SHRIMP (LITOPENAEUS VANNAMEI)) Widanarni, Widanarni; Sukenda, Sukenda; Septiani, Ghita Ryan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.787 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5041

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This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.493±0.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.93±0.025-6.97±0.019% and had higher values than controls (A and B) (P 0.05). Meanwhile, feed conversion value in C and D (1.54±0.142 and 1.58±0.117) were lower than controls (P 0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P 0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.
BIOFLOC TECHNOLOGY ON THE INTENSIVE AQUACULTURE OF BRONZE CORYDORAS ORNAMENTAL FISH CORYDORAS AENEUS WITH DIFFERENT STOCKING DENSITIES Diatin, Iis; Suprayudi, Muhammad Agus; Budiardi, Tatag; Harris, Enang; Widanarni, Widanarni
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3552.973 KB) | DOI: 10.19027/jai.18.2.202-213

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ABSTRACT Ornamental fish is non consumption fish which is an important source of Indonesian foreign exchange. The objective of this study is to analyze the productivity of bronze corydoras Corydoras aeneus ornamental fish through increased stocking density with biofloc technology. The average weight of the experimental corydoras was 0.61 ?0.72 g with 2.32?2.40 cm standard length. This study used a randomized design method with biofloc technology treatment in 3000, 4500, and 6000 fish/m2 stocking densities. The results showed that the daily length and weight-growth rate among treatments were not significantly different (P>0.05), while survival rate and the number of fish production on all treatments were significantly different (P<0.05). The water quality during the rearing period, such as temperature, pH, alkalinity, ammonia, nitrite, and nitrate, were in a tolerable range for corydoras culture. The total suspended solids tended to be higher due to higher stocking density. The best productivity using biofloc technology obtained from 6000 fish/m2 stocking density. Keywords: Biofloc technology, Corydoras aeneus, growth rate, stocking density, survival rate. ABSTRAK Ikan hias merupakan produk perikanan non konsumsi yang menjadi sumber devisa Indonesia yang cukup penting. Penelitian ini bertujuan untuk menganalisis produktivitas ikan hias koridoras melalui peningkatan padat tebar dengan teknologi bioflok. Ikan yang digunakan adalah ikan hias koridoras (Corydoras aeneus) berbobot 0,61?0,72 g dan panjang baku 2,32?2,40 cm. Penelitian ini menggunakan rancangan acak lengkap dengan perlakuan teknologi bioflok pada padat tebar 3000, 4500, dan 6000 ekor/m2. Hasil penelitian menunjukkan bahwa laju pertumbuhan harian panjang dan bobot antar perlakuan tidak berbeda nyata (P>0,05), sedangkan kelangsungan hidup dan jumlah produksi ikan pada semua perlakuan berbeda nyata (P<0,05). Nilai kualitas air selama pemeliharaan yakni suhu, pH, alkalinitas, amonia, nitrit, dan nitrat yang berada pada kisaran yang cukup baik untuk budidaya ikan. Total padatan tersuspensi cenderung tinggi akibat dari semakin tinggi padat tebar. Produktivitas terbaik pada budidaya ikan koridoras dengan teknologi bioflok adalah pada padat tebar 6000 ekor/m2. Kata kunci:  Corydoras aeneus, kelangsungan hidup, padat tebar, pertumbuhan, teknologi bioflok 
Isolation and Characterization of Lactic Acid Bacteria from Inasua Mahulette, Ferymon; Mubarik, Nisa Rachmania; Suwanto, Antonius; Widanarni, Widanarni
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 2 (2016): December
Publisher : Faculty of Biology, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.468 KB) | DOI: 10.22146/jtbb.16380

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Inasua is a traditionally product of wet salt fish fermentation produced by Teon, Nila and Serua (TNS) Communities in Central Maluku, Indonesia. The community made this fermented fish to anticipate the lean time when fisherman could not go to sea.  The  fish that used as inasua raw material is demersal fishes that live around coral reefs, such as Samandar fish (Siganatus guttatus), Gala-gala fish (Lutjanus sp.) and Sikuda fish (Lethrinus ornatus). The objective of the research was to isolate and characterize of bacterial indigenous in  Inasua from three producers in Seram Island. The measurement of pH from inasua samples were 5.9, 5.0 and 5.8, respectively. The highest number of lactic acid bacteria was found from  Gala – gala inasua was 2,5x107 cfu/g sample. Isolation of all isolates bacteria from inasua showed that a total of 7 isolates of bacteria was obtained  from Samadar inasua, 9 isolates from  Gala-gala inasua, and 7 isolates from  Sikuda inasua.  From a total of 23 isolates, only 6 isolates had characteristic as lactic acid bacteria that were Gram  positive, negative catalase, and cocci shape. The microscopic characteristics  of the isolates are coccid in pairs or uniforms which combine to form tetrads. Carbohydrate utilization test  of selected isolate by using API 50 CHB kit indicated that 13 carbohydrates are fermented by these isolates  after incubation for 48 hours. The research  was concluded that the dominant bacteria in inasua sample  is  cocci-lactic acid bacteria.Keywords : fermented fish, inasua, lactic acid bacteria, MRSA medium
Synodontis eupterus Larvae Masculinization Using Javanese Long Pepper Extract (Piper retrofractum) Wijaya, Prassetyo Dwi Dhany; Junior, Muhammad Zairin; Soelistyowati, Dinar Tri; Widanarni, Widanarni
Journal Omni-Akuatika Vol 13, No 1 (2017): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.451 KB) | DOI: 10.20884/1.oa.2017.13.1.224

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Synodontis eupterus male represents only 5-10 % of farmer total synodontis production, which limits male availability for reproduction purpose. A method widely used in overcoming the low male production was masculinization through synthetic hormones, which was restricted due to its adverse impact on the environment. Natural ingredients from plant were demonstrated in various studies to substitute the 17α-methyltestosteron hormone on masculinization. This research aimed at evaluating the effects of Javanese long pepper extract (JLP) through immersion on synodontis fish larvae. The research was conducted using factorial design with two faktor (4x2) consisted of eight treatments: 17α-methyltestosteron 2 mg L-1 (MT) immersion for 5 and 10 hours, JLP dose 0.125 mg L-1 (P1) immersion for 5 and 10 hours and dose 0.25 mg L-1 (P2) immersion for 5 and 10 hours compared to the control treatment without immersion (P0). A hundred of synodontis larvae of 10 days old after hatching each replication were used in the immersion treatments. The results showed that JLP treatments produced 25-40 % of male synodontis age four months, 1-2 % females and above 60 % intersex. While at age five months, the percentage of intersex fish decreased to 20-40 %, the female fish increased to  60-62 %, and male fish ranged 20-35 % in JLP compared to the control P0 (15 %). The dose of JLP 0.25 mg L-1 increased mortality of about 14-54 %.
Phytoremediation of Eel (Anguilla bicolor bicolor) rearing wastewater using amazon sword (Echinodorus amazonicus ) and water jasmine (Echinodorus palaefolius) Handajani, Hany; Widanarni, Widanarni; Budiardi, Tatag; Setiawati, Mia; Sujono, Sujono
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.996 KB) | DOI: 10.20884/1.oa.2018.14.2.541

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Management of aquaculture wastewater is still the major problems in fisheries, especially in intensive systems. Intensively aquaculture activities often increase organic matter and nutrients (N and P) in the water.  The study was conducted to evaluate the phytoremediator performance of Echinodorus amazonicus and Echinodorus palaefolius in removing inorganic nitrogen and orthophosphate from aquaculture wastewater with the recircula­tion system. This study used a completely randomized design with three treatments and 3 replications. The treatments of this study were P1: E. amazonicus,  P2: E. palaefolius and P3: control (without aquatic plants). The tested fish were elvers  which average weight 6.98 ± 0.19 g,  and  fish density was 4 gL-1. Elvers reared in an aquarium that containing 48 L in  a recirculation system. The paste feed which protein level of  45.25% was given at satiation 3 times/day. Elvers reared for 60 days. The results showed that E. palaefolius significantly reduced concentrations of total ammonia nitrogen (TAN), nitrite (NO2), nitrate (NO3) and orthophosphate (PO4) with an efficiency of 27.10 ± 2.42%; 45.03 ± 9.77%; 20.94 ± 1.29% and 14.19 ± 3.05%, respectively higher than E. amazonicus and control. The best result of elver’s performance (SGR and FCR) was in treatment P2 (E. palaefolius), i.e.  1.19 ± 0.18% and 1.57 ± 0.30. Based on the results of this study, it can be concluded that the use of E. palaefolius as phytoremediator in eel culture with recirculation system can removed inorganic nitrogen and orthophosphate more optimal compared to other treatment. 
Kinerja Pertumbuhan dan Respons Imun Larva Udang Vaname yang diberi Probiotik Pseudoalteromonas piscicida dan Prebiotik Mannanoligosakarida melalui Bioenkapsulasi Artemia sp. Hamsah, Hamsah; Widanarni, Widanarni; Alimuddin, Alimuddin; Yuhana, Munti; Junior, Muhammad Zairin
Proceeding Simposium Nasional Kelautan dan Perikanan No 5 (2018)
Publisher : Proceeding Simposium Nasional Kelautan dan Perikanan

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Pemberian probiotik, prebiotik, dan sinbiotik merupakan salah satu cara untuk meningkatkan pertumbuhan dan respons imun pada ikan, udang, dan organisme akuatik lainnya. Penelitian ini bertujuan mengevaluasi kinerja pertumbuhan dan respons imun larva udang vaname yang diberi probiotik Pseudoalteromonas piscicida (1Ub), prebiotik mannanoligosakarida (MOS), dan sinbiotik (kombinasi probiotik 1Ub dan prebiotik MOS) melalui bioenkapsulasi Artemia sp. Bioenkapsulasi dilakukan dengan cara menambahkan probiotik 1Ub konsentrasi 106 CFU/mL, prebiotik MOS 12 mg/L, dan sinbiotik (kombinasi 106 CFU/mL 1Ub dengan 12 mg/L MOS) pada media pemeliharaan Artemia sp. selama 4 jam. Pemberian Artemia sp. hasil bioenkapsulasi ke larva udang dilakukan selama 13 hari (Mysis3 sampai PL12). Pertumbuhan panjang dan bobot tubuh larva udang vaname diamati pada awal dan akhir penelitian, sedangkan rasio RNA/DNA, aktivitas enzim pencernaan, kelangsungan hidup, jumlah total bakteri, dan respons imun larva udang meliputi total hemosit (THC), aktivitas phenoloxidase (PO), dan aktivitas respiratory burst (RB) dianalisa pada akhir penelitian. Hasil penelitian menunjukkan laju pertumbuhan harian (DGR), panjang mutlak, rasio RNA/DNA, aktivitas enzim, kelangsungan hidup, jumlah total bakteri, dan respons imun pada larva udang yang diberi probiotik, prebiotik, dan sinbiotik berbeda nyata (p˂0,05) dibandingkan dengan kontrol. Pemberian sinbiotik menunjukkan hasil terbaik dengan DGR (24.39±0.31% per hari), panjang mutlak (13.00±0.50 mm), rasio RNA/DNA (0.6369±0.0094), aktivitas enzim pencernaan (protease 0.033±0.0007; lipase 0.047±0.0010; amilase 0.853±0.008; mananase 0.148±0.004 U/mL/menit), kelangsungan hidup (92.67±1.26%), jumlah total bakteri (6.7 x 107 CFU/0.1g larva), THC (7.6 x 106 sel/mL), aktivitas PO (0.19±0.002 OD 490 nm), dan aktivitas RB (0.67±0.028 OD 630 nm) yang lebih tinggi dibandingkan perlakuan lainnya dan kontrol. Kata Kunci: probiotik, prebiotik, sinbiotik, Artemia sp., udang vaname. 
Ablasi Tangkai Mata Mempercepat Pematangan Ovari Lobster Pasir (Panulirus homarus) pada Musim Pemijahan Adiputra, Yudha Trinoegraha; Junior, Muhammd Zairin; Suprayudi, Muhammad Agus; Manalu, Wasmen; Widanarni, Widanarni
Proceeding Simposium Nasional Kelautan dan Perikanan No 5 (2018)
Publisher : Proceeding Simposium Nasional Kelautan dan Perikanan

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Musim pemijahan alami lobster pasir di perairan Pesisir Barat Lampung terjadi pada Juli-September yang ditandai dengan hasil tangkapan induk betina yang membawa telur mendominasi saat ditangkap. Pelarangan penangkapan lobster pasir ukuran kecil diduga akan menganggu produksi penangkapan sehingga perlu dimulai pembenihan yang memperlukan teknik pemeliharaan dan maturasi ovari induk betina lobster pasir hasil tangkapan alam. Tujuan penelitian ini mempelajari cara pemeliharaan induk dan pengaruh ablasi satu tangkai mata pada maturasi ovari lobster pasir (Panulirus homarus). Empatpuluh delapan ekor lobster pasir digunakan dan dipisahkan menjadi 2 kelompok yaitu ablasi satu tangkai mata dan tanpa ablasi tangkai mata. Tiga ekor diambil dari setiap kelompok pada hari ke-1:3:7:10:14:21 dan 27 untuk evaluasi perubahan anatomi ovari dan gonadosomatik indeks (GSI). Hasil penelitian menunjukkan seleksi selama masa adaptasi induk dapat meniadakan kematian induk karena ablasi mata. Anatomi ovari menunjukkan terjadinya maturasi ovari yang meningkat dari matang awal menjadi matang berlebih dengan ablasi mata pada hari ke-3 setelah ablasi sampai hari ke-27. Berbeda dengan perlakuan diatas, induk betina tanpa ablasi mata tidak dapat mematangkan ovari secara spontan. Setelah 27 hari, ovari secara serentak mencapai matang berlebih yang diasumsikan karena nutrien dari pakan yang diberikan dapat mendukung maturasi ovari. Perbedaan GSI antara perlakuan ablasi satu tangkai mata berbeda nyata (P<0,05) dengan tanpa ablasi tangkai mata. Hal ini menunjukkan hilangnya hormon penghambatan vitelogenesis (VIH) dan terpacunya hormon stimulasi vitelogenesis (VSH) karena ablasi tangkai mata. Studi merekomendasikan adaptasi untuk seleksi induk, ablasi untuk memperoleh maturasi ovari yang singkat dibandingkan dengan tanpa ablasi mata meskipun dengan nutrien pakan yang berkualitas. Kata Kunci: ablasi mata, GSI, lobster pasir, maturasi ovari, musim pemijahan