Diah tri widayati
Laboratorium Fisiologi dan Reproduksi Ternak Fakultas Peternakan Universitas Gadjah Mada, Yogyakarta

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MPPA (MOST PROBABLE PRODUCING ABILITY) ESTIMATION OF KEBUMEN ONGOLE CROSSBRED CATTLE BASED ON OFFSPRINGS WEANING WEIGHT Sumadi, Sumadi; Ngadiyono, Nono; Widayati, Diah Tri; Noviandi, Cuk Tri; Fathoni, Akhmad; Khusnudin, Mukhamad
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6712.99 KB) | DOI: 10.22146/jsv.27561

Abstract

Tujuan dari penelitian ini yaitu untuk menghitung nilai MPPA induk pada sapi PO Kebumen. Penelitian ini telah dilakukan pada bulan Juli sampai dengan Oktober 2015 di wilayah Urut Sewu, Kebumen. Materi penelitian yang digunakan adalah data recording tiga tahun terakhir (2013-2015) terdiri dari 41 ekor pejantan, 51 induk dan 244 pedet. Data berat sapih anak sebelumnya telah dikoreksi berdasarkan umur induk, jenis kelamin dan umur penyapihan 120 hari. Ripitabilitas dihitung menggunakan metode korelasi antarkelas. Nilai MPPA induk dihitung berdasarkan nilai ripitabilitas berat sapih keturunan. Hasil penelitian menunjukkan bahwa nilai ripitabilitas berat sapih anak termasuk dalam kategori tinggi (0.32 ± 0.15). Nilai MPPA induk sapi PO Kebumen kemudian dihitung menggunakan nilai tersebut dan juga rata-rata performan populasi (83.45 kg). Dihasilkan 10 besar induk sapi PO Kebumen dengan nilai MPPA tertinggi. Nilai MPPA kemudian diurutkan dari nilai tertinggi ke terendah. Nilai MPPA tertinggi dimiliki oleh SAJ0315078 (110.69) dan terendah dimiliki oleh TA1112020 (97.20). Hasil dari penelitian ini kemudian dapat digunakan sebagai dasar dalam seleksi induk sapi PO Kebumen di dalam populasinya.
MUTU ARANG AKTIF DARI SERBUK GERGAJI KAYU Pari, Gustan; Widayati, Diah Tri; Yoshida, Masato
Jurnal Penelitian Hasil Hutan Vol 27, No 4 (2009): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2009.27.4.381 - 398

Abstract

Tulisan ini mengemukakan hasil penelitian pembuatan arang aktif  dari serbuk gergaji kayu, dengan tujuan untuk mengetahui pengaruh suhu dan lama waktu aktivasi terhadap hasil dan mutu arang aktif yang dihasilkan dari arang serbuk kayu gergajian. Proses pembuatan arang aktif dilakukan dengan menggunakan retor yang terbuat dari baja tahan karat yang dilengkapi dengan elemen listrik pada suhu 700, 800 dan 900 oC dengan lama waktu aktivasi masing-masing selama 30, 60 dan 90 menit. Bahan pengaktif yang digunakan adalah larutan H3PO4 15%. Hasil penelitian menunjukkan bahwa kondisi terbaik untuk membuat arang katif dihasilkan pada suhu 900oC, dengan lama waktu aktivasi 90 menit, yang menghasilkan rendemen sebesar 11,33%, kadar air 19,26%, kadar abu 41,90%, kadar zat terbang 9,25%, kadar karbon terikat 48,85%. Daya serap arang aktif  terhadap benzena sebesar 10,93%, CHCl3   sebesar 30,38%, daya serap iodium 1171,5 mg/g (memenuhi syarat standar Jepang) dan daya serap terhadap biru metilena sebesar 149,98 mg/g. Berdasarkan sifat dan besarnya daya serap terhadap biru metilena, maka arang aktif dari serbuk gergaji kayu ini dapat digunakan untuk penjernihan zat warna dan sebagai campuran pohon ternak.
Pengaruh Proteksi Aldehid Untuk Meloloskan Poly Unsaturated Fatty Acid Pada Menir Kedelai dan Minyak Ikan Lemuru Secara In Vitro Riyanto, Joko; Baliarti, Endang; Yusiati, Lies Mira; Hartatik, Tety; Widayati, Diah Tri
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 13, No 1 (2015): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.13.1.57-65

Abstract

This research was aims to determine the effect of formaldehyde using as a protective material in the soy groats and lemuru fish oil mixtured to escape of the protein and poly unsaturated fatty acid (PUFA) by in vitro. The rumen fluid was derived from donor of the Ongole crosbred fistulated as much as 3 heads. The experimental design used completely randomized design factorial 2x3 pattern consists of two factors:the first factor (soybean groats:lemuru fish oil in the ratio 2:1 and 4:1) and the second factor (the use of formaldehyde at levels of 0%, 2% and 4% base on the dry matter) and was repeated 5 times. The parameters are consist of the nutrient content, the substrates soybean groats and lemuru fish oil the rumen fluid fermented PUFA content by in vitro. Results of the study are levels of protein, fatty acids  oleic, linoleic and arachidonic much available on soybean groats:lemuru fish oil protected formaldehyde at levels of 2% and 4%. The difference between the ratio of soybean groats with lemuru fish oil had no significant (P≥0,05) but significantly different at different levels of formaldehyde (P≤0,05). The conclusion are formaldehyde content of 37% with a level of 2% can be used for protection the soybean groats and lemuru fish oil with a ratio of 4:1 and is able to provide protein as well PUFA. Keywords: soy groats, lemuru fish oil, in vitro, PUFA, formaldehyde protection
EVALUASI PAKAN SUPLEMEN MINYAK IKAN LEMURU DAN HIDROLISAT DARAH TERPROTEKSI BERDASARKAN KECERNAAN BAHAN KERING DAN KECERNAAN BAHAN ORGANIK DI DALAM RUMEN DAN PASCA RUMEN Pramono, Ahmad; Kustono, Kustono; Widayati, Diah Tri; Putro, P P; Hartadi, Hari
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 14, No 1 (2016): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.14.1.36-42

Abstract

Increased of ruminants energy intake can be conducted by increasing the energy density of feed by using fat (oil). Protein intake can be enhanced by increasing of protected protein that escapes degradation by rumen microbes. The Aim of this research was to evaluate protected of feed supplement based on dry matter digestibility (KcBK), organic matter digestibility (KcBO) in vitro in the rumen and post-rumen. Protected feed supplement was produced from sardine fish oil and hydrolyzed blood , throught two protection methodes. It was saponification and microencapsulation. This research uses a completely randomized design with 3 treatments (feed supplements protected, soybean meal and pangola grass), which each treatment was repeated 5 times. The results in the first step (rumen) of the protected feed supplement showed that has dry matter digestibility 24.46% and organic matter digestibility 57.17%, soybean meal has dry matter digestibility 75.20% and organic matter digestibility 75.66%, and pangola grass has dry matter digestibility 50 , 81% and organic matter digestibility 59.21%. In the second step (post rumen) the digestibility of protected feed supplement has dry matter digestibility 69.04% and organic matter digestibility 66.71 72.76%, soybean meal has dry matter digestibility 90.38% and organic matter digestibility 88.23%, and the grass pangola have KcBK 63.24% and KcBO 59.21%. The results could be concluded that in the rumen protected feed supplementation is the most microbial degradation resistant compared the other and it can be degraded and digested in the abomasum to the small intestine. 
MUTU ARANG AKTIF DARI SERBUK GERGAJI KAYU Pari, Gustan; Widayati, Diah Tri; Yoshida, Masato
Jurnal Penelitian Hasil Hutan Vol 27, No 4 (2009): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2009.27.4.381-398

Abstract

Tulisan ini mengemukakan hasil penelitian pembuatan arang aktif  dari serbuk gergaji kayu, dengan tujuan untuk mengetahui pengaruh suhu dan lama waktu aktivasi terhadap hasil dan mutu arang aktif yang dihasilkan dari arang serbuk kayu gergajian. Proses pembuatan arang aktif dilakukan dengan menggunakan retor yang terbuat dari baja tahan karat yang dilengkapi dengan elemen listrik pada suhu 700°C, 800°C dan 900°C dengan lama waktu aktivasi masing-masing selama 30, 60 dan 90 menit. Bahan pengaktif yang digunakan adalah larutan H3PO4 15%. Hasil penelitian menunjukkan bahwa kondisi terbaik untuk membuat arang katif dihasilkan pada suhu 900°C, dengan lama waktu aktivasi 90 menit, yang menghasilkan rendemen sebesar 11,33%, kadar air 19,26%, kadar abu 41,90%, kadar zat terbang 9,25%, kadar karbon terikat 48,85%. Daya serap arang aktif  terhadap benzena sebesar 10,93%, CHCl3   sebesar 30,38%, daya serap iodium 1171,5 mg/g (memenuhi syarat standar Jepang) dan daya serap terhadap biru metilena sebesar 149,98 mg/g. Berdasarkan sifat dan besarnya daya serap terhadap biru metilena, maka arang aktif dari serbuk gergaji kayu ini dapat digunakan untuk penjernihan zat warna dan sebagai campuran pohon ternak.
SUPPLEMENTATION OF FOLLICLE STIMULATING HORMON INTO IN VITRO MATURATION MEDIUM TO INCREASE OOCYTES MATURATION AND 4 CELL STADIUM EMBRYO DEVELOPMENT OF BLIGON GOAT Achadri, Yanuar; Bintara, Sigit; Widayati, Diah Tri
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.249 KB) | DOI: 10.21059/buletinpeternak.v42i2.13609

Abstract

The study was carried out to investigate the effect of follicle stimulating hormon (FSH) into in vitro maturation medium to increase oocytes maturation and 4 cell stadium embryo development of Bligon goat. Goat ovaries were obtained from a slaughterhouse and transported to the laboratory in a flask of NaCl at temperature of 31 ? 34°C. Oocytes were aspirated from 2 ? 6 mm of follicles into a 3 mL syringe (23G needle) that contained Dulbecco?s Phosphate-Buffered Saline. Oocytes were divided into three groups, i.e tissue culture medium (TCM) with FSH supplementation 0, 50, and 100 IU/mL. Oocytes were put into those medium and incubated on 39°C, 5% CO2, and 95% humidity for 24 hours. Matured oocytes were fertilized with capacitated frozen thawed-semen and incubated on 39°C, 5% CO2, and 95% humidity for 5 hours. Fertilized oocytes were washed for 3 times in TCM and incubated in the same condition for embryo culture. The data of FSH supplementation and embryo development were analyzed using randomized completely one way classification. The results showed that the percentages of mature oocytes from FSH supplementation 0, 50, and 100 IU/mL were 70,48±23,22, 78,48±15,80, and 80,29±12,86%, respectively. Cleavage rate of the two cells stage were 36,00±14,22, 44,00±33,94, and 57,45±31,78%, respectively, and for the 4 cells stage were 27,33±22,04, 35,33±40,73, and 39,45±20,38%. It is concluded that supplementation of FSH in the maturation medium could not increase the percentages of in vitro maturation and embryo development.
SUPLEMENTASI HORMON GONADOTROPIN PADA MEDIUM MATURASI IN VITRO UNTUK MENINGKATKAN PERKEMBANGAN EMBRIO STADIUM 4 SEL KAMBING BLIGON Pranatasari, Dewi; Kustono, Kustono; Widayati, Diah Tri
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.47 KB) | DOI: 10.21059/buletinpeternak.v40i2.9080

Abstract

The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality.