Irkham Widiyono
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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RESPONS TULANG FEMUR TIKUS OVARIOHISTEREKTOMI YANG MENGKONSUMSI KASEIN DAN DISUPLEMENTASI CALCITRIOL SELAMA 30 MINGGU (THE RESPONSE OF BONE FEMUR OVARIOHISTERECTOMIZED RATS CONSUMING CASEIN AND CALCITRIOL SUPPLEMENTATION FOR 30 WEEKS) Hartiningsih, Hartiningsih; Anggraeni, Devita; Widiyono, Irkham; Wuryastuty, Hastari
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Calcitriol supplementation in ovariohisterectomized rat was known to decreased calcium retention.The objective of the research was to study the response of femur bone to calcitriol supplementation for 30weeks in ovariohisterectomized rats consuming casein. Twenty female Wistar rats at 8 weeks of age wererandomly divided into four groups (unovariohisterectomy without calcitriol supplementation (N),unovariohisterectomy with calcitriol supplementation (ND), ovariohisterectomy without calcitriolsupplementation (O) and ovariohisterectomy with calcitriol supplementation (OD) of five each. Thirtyweeks after surgery, femur was taken for histopathological and immunohistochemistry examination.Immunohistochemistry of distal femur metaphysis in group O and OD were revealed decreasing tartrateresistant alkaline phosphatase 5b (TRAP5b) in trabecular bone, which was located in bone marrow space,and also in trabecular speculum surface. Histopathological analysis of distal femur metaphysis in groupN and ND were showed normal structure, meanwhile, distal femur metaphysis in group O and OD wereshown some abonormalities, such as increased of bone marrow space, domination of adipocytes in the bonemarrow, and decreased of trabecular bone speculum in metaphysis. Based on the results, it was concludedthat femur bone of ovariohisterectomized rats fed casein with and without calcitriol 8ng/daysupplementation for 30 weeks were showed unbalanced between resorption and formation of bone whichwas domination by bone resorption.
KONSUMSI, KECERNAAN NUTRIEN, PERUBAHAN BERAT BADAN DAN STATUS FISIOLOGIS KAMBING BLIGON JANTAN DENGAN PEMBATASAN PAKAN Suwignyo, Bambang; Wijaya, Ulil Amri; Indriani, Rieska; Kurniawati, Asih; Widiyono, Irkham; Sarmin, Sarmin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6704.108 KB) | DOI: 10.22146/jsv.27560

Abstract

This study were aimed to determine the dry matter and organic matter intake, nutrients digestibility, body weight gain and physiological status of male Bligon goats treated with feed restriction. Six Bligon goats average age of 12 months and an average body weight of 23.6 kg were fed 40% of peanut raughages (rendeng) and 60% concentrate. Goats were divided into two (2) treatment groups. Control goat treatment were fed diets based on dry matter (DM) requirements 3.5% of body weight, and feed restriction goat treatment were fed diets with 50% reduction of DM requirement. Feed restriction was conducted for 35 days. The variables measured were dry matter (DM) and organic matter (OM) intake, total digestibility nutrient (TDN) intake, body weigh gain, dry matter (DM) and organic matter (OM) digestibility, rectal temperature, respiration frequency, and heart rate. Data were analyzed by independent samples T-test. The results showed that the feed restriction significantly decreasedconsumption of DM (676.07 ± 14.76 into 372.30 ± 53.08) and OM (639.38 ± 15.89 into 349.88 ± 49.35), consumption of TDN (461.60 ± 10.23 into 253.99 ± 36.17), DM digestibility (74.39 ± 1.60 into 71.27 ± 0.89) and OM digestibility (79.42 ± 1.54 into 76.34 ± 0.24), but had no significant effect on body  temperature, respiration frequency and heart rate (pulsus) of male Bligon goat. Value of ADG was 55.24 g/day (P0) then increased to 131.43 g/day when the feed was filled back. A feed restriction up to 50% decreased goat production performance, but did not interfere with the health status of livestock (can be indicated from physiological status of goat still in the normal range).
RESTRIKSI PAKAN YANG DIIKUTI DENGAN REFEEDING MENURUNAN LEVEL FOSFAT INORGANIK DAN KALSIUM PADA KAMBING KACANG JANTAN DEWASA (FEED RESTRICTION FOLLOWED BY REFEEDING DECREASED THE INORGANIC PHOSPHATE AND CALCIUM LEVELS IN ADULT KACANG GOATS) Sarmin, Sarmin; Widiyono, Irkham; Astuti, Pudji; Putro, Prabowo Purwono
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.2.297

Abstract

The objective of this research was to evaluate metabolism of inorganic phosphat (Pi) and calcium (Ca) in kacang goats subjected to 60-d feed restriction and 60-d full feeding (refeeding). Nine adult male goats were divided into three groups used in this study. Restrictions include 40% (group I), 50% (group II), and 60% (group III) of the ration of dry fodder. To obtain the baseline all animals from all groups were fullfeeding from the start of the study (day 0) to day 60 then followed by restriction stage on day 61 to day 120. The next stage is refeeding from day 121 to 180. Pi and Ca levels were analyzed on days 60, 120, and 180, respectively. The results showed that restriction followed by refeeding decreased Ca levels in all three groups and Pi of animals in groups II and III was below the baseline. The level of Pi reach above the baseline were seen only in animals in group I following restriction and refeeding, although there was a tendency to decrease after refeeding. Based on the results of the study it is concluded that the restriction followed by refeeding decreases the level of Pi and Ca in adult male kacang goats. ABSTRAK Tujuan penelitian ini adalah mengkaji metabolisme fosfat inorganik (Pi) dan kalsium (Ca) selama 60 hari restriksi (pembatasan) pakan yang diikuti dengan 60 hari pemberian pakan kembali secara fullfeeding (refeeding) pada kambing kacang jantan dewasa. Sembilan ekor kambing kacang jantan dewasa terbagi menjadi tiga kelompok digunakan dalam penelitian ini. Restriksi meliputi 40% (kelompok I), 50% (kelompok II), dan 60% (kelompok III) dari 3,5% bahan kering dari bobot badan. Tahap awal sebagai baseline dimulai dengan pemberian pakan fullfeeding pada hari ke-0 sampai hari ke-60 dilanjutkan dengan tahap restriksi dimulai pada hari ke-61 sampai hari ke-120. Tahap berikutnya adalah refeeding mulai hari ke-121 sampai dengan hari ke-180. Level Pi dan Ca dianalisis pada hari ke-60, 120, dan 180. Hasil penelitian menunjukkan bahwa restriksi yang diikuti refeeding menurunkan level Ca pada ketiga kelompok dan Pi kelompok II dan III di bawah baseline. Level Pi berada di atas baseline hanya pada kelompok I setelah restriksi dan refeeding, meskipun cenderung menurun setelah refeeding Berdasarkan hasil penelitian disimpulkan bahwa restriksi yang diikuti dengan refeeding menurunkan level Pi dan Ca pada kambing kacang jantan dewasa.  
KETERKAITAN PANHISTEREKTOMI DAN SUPLEMEN 1,25- DIHIDROKSIVITAMIN D3 DENGAN RISIKO UROLITIASIS PADA TIKUS (CORRELATION BETWEEN PANHISTERCTOMY AND 1.25-DIHYDROXYVITAMIN D3 SUPPLEMENTATION ON RATS UROLITHIASIS RISK) ., Hartiningsih; Anggraeni, Devita; Widiyono, Irkham; Wuryastuti, Hastari
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this research was to study the correlation of panhisterectomy and supplement 1.25-dihydroxyvitamin D3 on urolithiasis risk in Wistar rats. Twenty female Wistar rats at 8 weeks of age, weredivided into four groups (control fed standard diet, control fed standard diet+1,25-dihydroxyvitamin D3 supplement, panhisterectomy fed standard diet and panhisterectomy fed standard diet +1,25-dihydroxyvitamin D3 supplement). Eleven weeks after treatment, each of rats was placed into individualmetabolic cage for balance study for a week. From day 4 to 11 of the balance study, every morning theremaining food, feces, and urine were collected and recorded for calcium (Ca) analysis. At the end ofbalance study, blood samples were taken from canthus retroorbitalis medialis for estrogen analysis. Theresults showed urinary and fecal Ca excretions were not significantly different compared to the controlgroup. Calcium consumption was significantly higher (P<0.05) in panhisterectomized rats compared withthose in control rats. While, estrogen in panhisterectomized group was not significantly different to thosein control rats. Calcium urinary and Ca consumption in rats consuming 1,25-dihydroxyvitamin D3 supplement were significantly higher (P<0.05) compared with those in without 1,25-dihydroxyvitamin D3 supplementation, but Ca excretion in feses was not significantly different. Estrogen in rats consuming1.25-dihydroxyvitamin D3 supplement was significantly lower (P<0.05) compared with the rats that without1,25-dihydroxyvitamin D3 supplemention. It can be concluded that panhisterectomy does not seem to affecturolithiasis risk, while 1,25-dihydroxyvitamin D3 supplement may affect urolithiasis risk. There is likelyno association between panhisterectomy and 1.25-dihydroxyvitamin D3 supplementation on urolithiasisrisk in Wistar rats.
PEMBERIAN PAKAN BAHAN KERING BERKUANTITAS TERBATAS SELAMA EMPAT MINGGU TIDAK MENGANGGU KESEHATAN DAN REPRODUKSI KAMBING KACANG JANTAN DEWASA (FEEDING WITH A RESTRICTED QUANTITY OF DRY MATTER OVER FOUR WEEKS IS NOT DETRIMENTAL TO HEALTH AND REPRODUCTION IN Widiyono, Irkham; Suwignyo, Bambang; Sarmin, Sarmin; Susmiyati, Trini
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study was aimed to investigate the effects of restricted feeding for four weeks on some bloodchemistry and seminal parameters in kacang goats. Six adult clinically healthy male kacang goats wereused in this study. Each animal was kept in individual box for 12 weeks. Animal was fed with aerial partof peanut plant and concentrate with ratio of 60:40. The experiment was devided into two feeding periods,fullfeeding and restricted feeding. After four weeks adaptation (week 1-4), each animal was fullfed formaintenance plus medium activity (a quantity of dry matter at the level of 3% of body weight) for fourweeks (week 5-8) and then was fed only 50% of the fullfeeding nutritional level for the following four weeks restricted feeding period (week 9-12). During the experiment, drinking water was supplied ad libitum.Each animal was ejaculated at weekly intervals by means of standard artificial vagina for small ruminant.Blood and semen samples were collected at the end of the ad libitum and restricted feeding period for bloodchemistry and seminal parameters analyses. Statistical differences were determined by paired t-test. A0.05 probability level was used as criterion to describe statistically significant differences. Restrictedfeeding at the dry matter intake level of 1.5% body weight for four weeks did not result in significantchanging of serum glucose and total protein concentrations as well as seminal parameters. The level ofblood chemistry and seminal parameters during the feed restriction period were within the values reportedfor clinically healthy goats. It is concluded that feeding with a restricted quantity of dry matter at the levelof 1.5% of body weight for four weeks is not detrimental to health and reproduction in male kacang goats.
PENGARUH PEMBATASAN PAKAN DAN PEMENUHAN KEMBALI PAKAN TERHADAP KONSUMSI NUTRIEN KECERNAAN NUTRIEN, STATUS FISIOLOGI DAN PERUBAHAN BERAT BADAN KAMBING BLIGON Suwignyo, Bambang; Wijaya, Ulil Amri; Indriani, Rieska; Kurniawati, Asih; Widiyono, Irkham; Sarmin, Sarmin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.11603

Abstract

Penelitian ini bertujuan untuk mengetahui kecernaan nutrien pakan dan status fisiologi pada kambing Bligon yang diberi perlakuan pembatasan pakan (feed restriction). Enam ekor kambing Bligon jantan umur rata-rata 12 bulan dengan bobot badan rata-rata 23,6 kg diberi pakan yang terdiri dari hijauanjerami kacang tanah (rendeng)dan konsentrat. Kambing dibagi menjadi 2 kelompok perlakuan. Tiga ekor kambing untukperlakuan kontrol diberi pakan berdasarkan kebutuhan bahan kering (BK) 3,5% dari bobot badan, dan tiga ekor kambing untuk perlakuan pembatasan pakandilakukan pembatasan pakan dengan pengurangan pakan 50% dari kebutuhan bedasarkan BK selama 35 hari. Variabel yang diamati adalah konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), perubahan berat badan, kecernaan bahan kering (BK), kecernaan bahan organik (BO), temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung. Data yang diperoleh dianalisis dengan independent samplesT-Test. Hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa pembatasan pakan (feed restriction) berpengaruh nyata terhadap penurunan konsumsi bahan kering (BK), konsumsi bahan organik (BO), konsumsi total digestible nutrients (TDN), kecernaan bahan kering (BK) dan bahan organik (BO), tetapi tidak berpengaruh nyata pada temperatur tubuh, frekuensi respirasi, dan frekuensi detak jantung (pulsus) kambing Bligon. Pemenuhan kembali (refeeding) pakan berpengaruh nyata (P<0,05) menaikan kembali konsumsi BK dan konsumsi BO. Perubahan berat badan kambing mengikuti pola jumlah pemberian pakan. Saat pembatasan pakan, berat badan kambing perlakuan turun 55,24 g/hari dan ketika pakan dipenuhi kembali berat badannya meningkat 131,43 g/hari.
AKTIVITAS BEBERAPA ENZIM SERUM PADA KAMBING ETTAWA Widiyono, Irkham; S, Sarmin; Suwignyo, Bambang
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1259

Abstract

Penelitian ini bertujuan mengkaji aktivitas enzim alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), dan creatinine kinase (CK) pada kambing peranakan Ettawa. Sebanyak 43 ekor kambing peranakan Ettawa dari berbagai kelompok status fisiologis (jantan muda, betina muda, jantan dewasa, betina dewasa, betina bunting, dan betina laktasi) yang secara klinis sehat dan tidak cacat digunakan dalam penelitian ini. Pemeriksaan klinis dan pengambilan sampel darah dilakukan sesuai dengan skedul pemeriksaan dan pengambilan sampel sesuai dengan standard klinik yang diuraikan Baumgartner (1999). Pengambilan sampel darah dilakukan pada jam 7.00-8.00 wib. Pemeriksaan enzim di dalam serum diperiksa dengan menggunakan metode standar sebagaimana diuraikan oleh Kraft dan Duer (1999). Aktivitas AST, ALT, ALP di dalam serum memiliki level yang sebanding dengan level pada ruminansia lain. Sementara itu, aktivitas CK lebih tinggi dibanding nilai yang dinyatakan di dalam literatur untuk ruminansia kecil lainnya.
IDENTIFIKASI SEROVAR PENYEBAB LEPTOSPIROSIS PADA ANJING DI YOGYAKARTA Mulyani, Guntari Titik; Hartati, Sri; Wuryastuty, Hastari; Tjahajati, Ida; Yuriadi, Yuriadi; Widiyono, Irkham; Yanuartono, Yanuartono; Purnamaningsih, Hary; Indarjulianto, Soedarmanto; Raharjo, Slamet; Nururozi, Alfariza; Ganapragasam, Angeline; Jiao, Yeo Suan
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.014 KB) | DOI: 10.22146/jsv.39201

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Leptospirosis is a zoonotic disease of global concern, and is caused by pathogenic serovar Leptospira interrogans. Canine Leptospirososis is widespread worldwide, dogs can act as incidental hosts or maintenance hosts for various serovars. The purpose of this research was to identify leptospire serovars that infect healthy and suspected leptospirosis dogs in Yogyakarta. A total of 56 dogs (36 healthy dogs and 20 suspect leptospirosis dogs) sera were taken from cephalica vein as much as 3 ml. Sera were examined for leptospirosis with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospire serovar, namely: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. The results showed that Celledoni serovars infected 25% of healthy dogs and 5% of suspect leptospirosis dogs, Javanica serovar infected 19% of healthy dogs, Bataviae serovars infected 15% of suspect leptospirosis dogs, Grippotyphosa serovar infected 11% of healthy dogs, Tarrasovi serovar infected 10% of suspect leptospirosis dogs, serovars Cynopteri infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, serovar Pyrogenes infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, and serovar Rachmati infects 5% of suspect leptospirosis dogs. Seven healthy dogs (19%) and 2 suspect leptospirosis dogs (10%) were infected with more than 2 leptospire serovars. From the results of this study it can be concluded that Celledoni serovar of Leptospira interrogans infection causes subclinical leptospirosis, while Bataviae serovar infection causes clinical leptospirosis in dogs in Yogyakarta.
RESPONS STRES SAPI BALI JANTAN PADA PROSES PENGANGKUTAN DAN PEMUATAN KE ATAS KAPAL TERNAK (STRESS RESPONSE OF BALI BULLS DURING TRANSPORTATION AND LOADING PROCCESS ONTO THE CATTLE SHIP) Widayati, Sri; Astuti, Puji; Widiyono, Irkham
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.4.566

Abstract

Transportation is needed for livestock distribution from production centers to consumption centers. This might potentially result in a stressful condition for the cattle. The study was aimed to observe the stress response of Bali bulls during the transport and loading proccess onto the ship. Six adult clinically healthy Bali bulls were used in this study. The animals were housed in pen at the animal quarantine installation, given dried straw and drinking water ad libitum since three days before the study. The animals were loaded on the truck in 30 minutes, transported to the harbor in 15 minutes, and loaded on to the ship in 30 minutes. On the ship the animals were kept in a pen bedded with straw and given dried straw and drinking water ad libitum for 19 hours. Venous blood samples were collected from jugular vein during the rest in pen before loading on to the truck, after loading on the ship, and about 19 hours after loading and resting in the ship pen. Serum was separated by centrifugation and stored at -20oC for cortisol analysis. The concentrations of cortisol was analyzed by using enzyme linked immunosorbent assay (ELISA).&nbsp;The results showed that the average of serum cortisol concentration of Bali bulls was at the level of 33.81±28.52 ng/mL in the rest period at the quarantine installation, increased significantly to the level of 138.41±102.57 ng/mL after loading onto the ship (P&lt;0.05), and decreased again to the level of 41.54±43.47 ng/mL after 19 hours of resting period in the pen on the ship (P&gt;0.05). It can be concluded that Bali bulls may suffer from stress during transport and loading proccess which can be recovered after resting in the ship pen.&nbsp;
Pemberdayaan Peternak Marginal: Studi Kasus di Wilayah Banguntapan Bantul Widiyono, Irkham; Sarmin, Sarmin
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.851 KB) | DOI: 10.22146/jpkm.27031

Abstract

Over the recent years, villagers in the countryside havefaced the problem of limited resources for livestock and crop productions. Decreased land quality and agricultural land areawith respect to the increased need for housing landhas resulted in economic and environmental problems for livestock farmers in this area. Most livestock farmers have poor standard of education, limited ownership of land and livestock, and  are not capable of managing the natural resources in sustainable ways. The present services for small livestock farmersare aimed to transform the unintegrated livestock and agricultural farming into  a sustainable integrated system in the marginal community. Some efforts were conducted to improve knowledge and skills in zero waste system in livestock and agricultural farming by livestock farmers in marginal areas. The present community development programs were conducted using farmer-centered learning methods (participative learning) and mentoring by professionals on the implementation of sustainable integrated farming system (learning by practice). Furthermore, institutional development and cooperative networking with related parties were carried out. Results showed that the livestock farmerswere able to adopt integrated farming practices and the community was capable of independently producing organic fertilizer from livestock wastes and converting agricultural byproducts into animal feed. Both farmers and villagers have come together to cultivate fruit and vegetable plantations in vacant lands and back yards which were previously unused. The farmers’ group has successfully networked with the related partners to ensure sustainability of environmental conservation efforts and improvement of livelihood. In conclusion, active participation of farmersin the marginal area throughout the development process and mentored-practice are key factors for successful agricultural transformation in the marginal community. Implementation of the sustainable integrated farming system will ensure food security, environmental protection and safety, conservation of natural resources, and better quality of life.