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IDENTIFIKASI DAN SINERGISME KAPANG LIPOLITIK DARI LIMBAH SBE (SPENT BLEACHING EARTH) SEBAGAI AGEN BIOREMEDIASI Mukharomah, Ervina; Munawar, Munawar; Widjajanti, Hary
Jurnal Ilmu Lingkungan Vol 13, No 1 (2015): April 2015
Publisher : Program Studi Ilmu Lingkungan,Program Pascasarjana, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.226 KB) | DOI: 10.14710/jil.13.1.19-26

Abstract

Identifikasi dan sinergisme lipolitik dari Limbah SBE (Spent Bleaching Earth) yang berpotensi sebagai agen bioremediasi telah dilakukan pada bulan Januari-Maret 2015. Pengambilan sampel dengan metode Random Sampling dari PT Wilmar International Group Mariana Sumatera Selatan. Penelitian dilakukan di Labolatorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Universitas Sriwijaya. Penelitian ini bertujuan untuk memperoleh isolat kapang lipolitik yang berpotensi mendegradasi residu minyak dan sebagai agen remediasi. Hasil penelitian ini diperoleh kapang lipolitik yang mampu mendegradasi minyak yaitu Cylindrocladium sp (V2), Fumago sp (V5) dan Aspergilus Fumigatus (V8). Ketiga isolat kapang ini dapat menurunkan residu minyak sebesar 76,6 %. Kapang yang memiliki potensi tinggi dalam mendegradasi minyak yaitu Aspergilus Fumigatus (V8).
EKSPLORASI BAKTERI ANTAGONIS ASAL JARINGAN DAN RIZOSFER TANAMAN KARET UNTUK MENEKAN PERTUMBUHAN BAKTERI PROTEOLITIK PADA BAHAN OLAHAN KARET (BOKAR) Gofar, Nuni; Munawar, Munawar; Widjajanti, Hary; Mulya, Angga Prasetya
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 2 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.176 KB) | DOI: 10.29244/jitl.16.2.61-66

Abstract

Lateks merupakan komoditas pertanian andalan dan merupakan sumber penerimaan devisa negara Indonesia yang cukup penting, namun pada saat penyimpanannya menyumbangkan bau tidak sedap ke udara. Penelitian ini bertujuan untuk mengeksplorasi bakteri antagonis asal jaringan dan rizosfer tanaman karet yang mampu menekan pertumbuhan mikroba proteolitik penyebab bau tidak sedap pada bahan olahan karet (bokar). Bakteri proteolitik penyebab bau tidak sedap diisolasi dari berbagai masa simpan bokar. Bakteri antagonis diisolasi dari jaringan dan rizosfer tanaman karet. Kemampuan bakteri antagonis menekan pertumbuhan bakteri proteolitik dilakukan dengan uji daya hambat dalam medium agar (NA). Diameter zona bening yang terbentuk merupakan indikasi kemampuan bakteri antagonis dalam menghambat aktivitas bakteri proteolitik. Dari hasil penelitian ini diperoleh 2 isolat bakteri yang berpotensi dikembangkan sebagai bakteri antagonis penghambat aktivitas bakteri proteolitik perombak bokar dalam proses penyimpanan. Kedua bakteri antagonis tersebut adalah isolat D1U1 yang berasal dari daun tanaman karet dan isolat A2U2 yang berasal dari akar tanaman karet, yang menghasilkan rata-rata zona hambat terluas berturut-turut berdiameter 11.37 dan 11.29 mm.
PERTUMBUHAN DAN HASIL PADI PADA INSEPTISOL ASAL RAWA LEBAK YANG DIINOKULASI BERBAGAI KONSORSIUM BAKTERI PENYUMBANG UNSUR HARA Wuriesyliane, Wuriesyliane; Gofar, Nuni; Madjid, Abdul; Widjajanti, Hary; Putu SR, Ni Luh
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 2, No 1 (2013)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.646 KB) | DOI: 10.33230/JLSO.2.1.2013.32

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The aim of this research was to study the effect of multisynergism bacterial isolates growth of rice plants as well as to obtain best composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria in spurring growth of rice plants. This research was conducted in October 2012 to February 2013. This study arranged in a completely randomized design (All treatments of multiple bacterial isolates resulted in increase of soil N x P Content). The results showed that multibacterial isolates B (endophytic bacteria + Azospirillum) increased vegetative and generative growth of rice plants. Treatment G (composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria) can increase rice yields. Some soil N and P content increased in response to all treatments of multiple bacterial isolates. The similar result was also observed in the N and P uptake that some multiple bacterial isolates improved N and P uptake of plants
Stimulate The Growth of Rice Using Endophytic Bacteria from Lowland Rice Plant Tissue Gofar, Nuni; Widjajanti, Hary; Marlina, Neni
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 12, No 2 (2015)
Publisher : Faculty of Agriculture, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v12i2.250

Abstract

Exploration and selection of endophytic bacteria from healthy food crops grown in lowland ecosystem is important to be conducted in order to get growth-stimulating endophytic bacteria at soil with low fertility level so that capable to optimize initial growth of food crops and subsequently can increase productivity level of lowland soil.The research objective was to isolate and to test the IAA-producing endophytic bacteria isolate in stimulating the rice crop growth at lowland area. Endophytic bacteria are isolated from tissues of rice, corn and peanut crops which grown at shallow swamp land in Ogan Ilir and Ogan Komering Ilir Districts, South Sumatra, Indonesia. There was nine isolates of nitrogen-fixer endophytic bacteria that capable to contribute IAA phytohormone into their growth media. The P31 isolate from rice crop tisssue of 2 months old produce the best rice sprouts than other isolates. This isolate can contribute of about 10 mg kg-1 IAA to its growth medium  and increase the crowns dry weight and the roots dry weight respectively with magnitudes of 133% and 225% compared to control treatment. Concentration and absorbtion of N for rice crops innoculated with P31 isolates had increased by 169% and 400%, recpectively. The P31 isolates had been identified as Burkholderia pseudomallei (also known as Pseudomonas pseudomallei).
Pengaruh MVA Gigaspora Margarita dan Bakteri Pelarut Fosfat Bacillus Megaterium Terhadap Tanaman Kedelai Widjajanti, Hary
Jurnal Penelitian Sains No 10 (2001)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (825.534 KB)

Abstract

Mikoriza berperan dalam meningkatkan serapan unsur hara bagi tanaman terutama unsur P dan bakteri fosfat mampu melarutkan fosfat yang ada di dalam tanah sehingga menjadi tersedia bagi tanaman. Tujuan penelitian adalah untuk mengetahui pengaruh mikoriza vasikular arbuskular (MVA) Gigaspora margarita dan bakteri pelarut fosfat (BPF) Bacillus megaterium terhadap tanaman kedelai yang ditanam di tanah Ultisol. Penelitian dilakukan di kebun dan Laboratorium Mikrobiologi FMIPA Unsri dengan menggunakan rancangan acak lengkapdengan pola faktorial dengan faktor I adalah dosis inokulum MVA yang terdiri atas: tanpa inokulasi BPF, 5 ml suspensi BPF dan 10 ml suspensi BPF. Kombinasi perlakuan diulang tiga kali dan pengamatan diulang 2 (dua) kali yaitu: pada fase R5 dan pada fase produksi. Hasil penelitian menunjukkan bahwa fase R5 inokulasi ganda MVA dan bakteri pelarut fosfat meningkatkan persentase infeksi MVA, serapan P tanaman, dan berat kering tanaman. Pada akhir fase produksi inokulasi ganda MVA dan bakteri pelarut fosfat tidak berpengaruh terhadap berat kering biji per tanaman. Inokulasi ganda MVA dan bakteri pelarut fosfat meningkatkan kadar P tanah.
Skrining Bakteri Kitinolitik Antagonis Terhadap Pertumbuhan Jamur Akar Putih (Rigidoporus lignosus) dari Rizosfir Tanaman Karet Muharni, Muharni; Widjajanti, Hary
Jurnal Penelitian Sains Vol 14, No 1 (2011)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1610.825 KB)

Abstract

Skrining bakteri kitinolitik dari rizosfir tanaman karet dilakukan dengan tujuan untuk memperoleh isolat bakteri kitinolitik yang antagonis terhadap pertumbuhan jamur akar putih (Rigidoporus lignosus). Bakteri diisolasi dari Perkebunan Karet Sembawa Kab. Banyuasin, dan skrining dilakukan dengan menggunakan media agar kitin dan uji antagonis terhadap jamur patogen dilakukan dengan menggunakan metode Chernin et al., (1995). Hasil penelitian didapatkan dua isolat bakteri kitinolitik yang antagonis terhadap pertumbuhan jamur akar putih (Rigidoporus lignosus). Identifikasi kedua isolat antagonis jamur akar putih ini tergolong kedalam genus Bacillus yaitu Bacillus sp. dan Bacillus apiarius
Pemanfaatan Rumput Fimbrisylis sp. dalam Proses Bioremediasi Tanah pada Berbagai Konsentrasi Limbah Minyak Bumi Pertiwi E, Sri; Widjajanti, Hary; Yudono, Bambang; Wahyudi, Hary
Jurnal Penelitian Sains Vol 14, No 1 (2011)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Penelitian pemanfaatan rumput Fimbristylis sp dalam proses bioremediasi bertujuan untuk mengamati pengaruh pemanfaatan Fimbristylis sp. terhadap jumlah populasi bakteri dan persentase penurunan TPH pada proses bioremediasi. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) berpola faktorial yang terdiri dari 2 faktor. Faktor 1: kosentrasi limbah minyak bumi yang terdiri dari 5 taraf: C0:0%, C1:5% , C2:10%, C3:15% dan C4: 20% Faktor 2 Penggunaan rumput Fimbrisylis sp yang terdiri dari 2 taraf : R0 : Tanpa menggunakan rumput Fimbristylis sp. dan R1: menggunakan rumput Fimbristylis sp., diperoleh 10 kombinasi perlakuan, masing-masing diulang 3 kali. Hasil penelitian menunjukkan pemanfaatan Fimbristylis sp. meningkatkan jumlah populasi bakteri dan penurunan nilai Total Petroleum Hidrokarbon (TPH). Namun semakin banyak limbah minyak bumi yang ditambahkan maka pertumbuhan Fimbristylis sp. semakin rendah 
Bioremediation of Spent Bleaching Earth (SBE) Wastes using Lipolitic Bacteria (Bacillus cereus) with Variation of Inoculum Volume Lusia, Maria; Gofar, Nuni; Widjajanti, Hary
Science and Technology Indonesia Vol 3 No 1 (2018): January
Publisher : ARTS Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.782 KB) | DOI: 10.26554/sti.2018.3.1.35-40

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Spent bleaching earth (SBE) is a solid waste was generated from the CPO refining step into cooking oil.  SBE that was discharged directly into the environment has the potential to pollute the environment, because in the SBE waste contained oil and acid residues, which are easily to be oxidized and flammable.  Therefore, SBE must be processed first before being discharged into the environment.  One way to manage SBE is by bioremediation.  Bioremediation is a method on cleaning the environment from contaminants by using  biological agents, such as bacteria, fungi etc.  The bacterial isolates used in this study were Bacillus cereus.  This study aims to obtain the best inoculum and to know the ability of Bacillus cereus bacteria in degrading the oil content in SBE waste. This study used Completely Randomized Design with the volume of Bacillus cereus bacteria inoculum as a treatment, consisting of 6 treatment levels of 0 mL kg-1, 25 mL kg-1, 50 mL kg-1, 75 mL kg-1, 100 mL kg-1, 125 mL kg-1.  Each treatment level was repeated 3 times, so taht 18 experimental units were obtained.  Observation was done once a week, in a month.  Parameters observed were bacterial population, percentage of oil degradation, and oil content degradation.  The best treatment result for the bacterial population was obtained at the treatment of 100 mL kg-1, at week 4 which was 7,4 x 108 cfu g-1, and for the oil degradation was obtained at 50 mL kg-1 on the treatment at week 4 as big as 90,43%.
THE INFLUENCE OF DOLOMITE GIVING ON INDIGENOUS BACTERIA NITROGEN INHIBITOR IN TIDAL SOIL HAS BEEN MANAGE FOR OVER 30 YEARS Ridwan, Rahmawati; Budianta, Dedik; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol 3, No 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1112.039 KB) | DOI: 10.24233/BIOV.3.2.2017.70

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The study was conducted to determine the effect of land typology and lime on the population of indigenous nitrogen-fixing bacteria and soil pH, and to analyze the effect of soil typology interaction and lime on the population of indigenous nitrogen-fixing bacteria and soil pH. Sampling of soil in tidal land of Mulya Sari and Banyu Urip Village of Banyuasin Regency, South Sumatera. Then the sample was studied at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Inderalaya. During January-May 2017. Based on the results of the study known the soil typology of the total population of nitrogen-fixing bacteria, the highest population of nitrogen inhibitor bacteria in the second week of soil typology D was 6.0x107 and the highest pH of 5.18 in typology B on week Third, the lime treatment that affected the population of indigenous nitrogen-fixing bacteria, obtained the population of bacteria as much as 9.3x105 in the second week and the highest soil pH 4.93 in the second week, while the results of the interaction between soil typology and lime treatment only affect the population of bacterial inhibitor Indigenous nitrogen, with bacterial population of 3.5x107 in soil typology D in the second week, but this interaction did not significantly affect soil pH, with the highest pH measurement of 5.33 on the soil typology D.Keywords: nitrogen-fixing bacteria, tidal land, lime treatment
ANTIBACTERIAL ACTIVITY OF THE SECONDARY METABOLITES PRODUCED BY ENDOPHYTIC FUNGI ISOLATED FROM ACANTHUS ILICIFOLIUS PLANT Widayanti, Gusti Ayu; Widjajanti, Hary; Salni, Salni
Indonesian Journal of Environmental Management and Sustainability Vol 3 No 4 (2019): December
Publisher : ARTS Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2558.148 KB) | DOI: 10.26554/ijems.2019.3.4.107-116

Abstract

Twelve endophytic fungi were isolated from the Acanthus ilicifolius plant. Two of them have the best antibacterial activity, namely Culvularia lunata and Diaporthe phaseolorum. Fungi can produce secondary metabolites that have the potential as antibacterial compounds. This study aims to isolate antibacterial compounds using column chromatography methods and test the antibacterial activity using diffusion methods to use paper discs. Isolation of secondary metabolites produces 27 eluates. From TLC, it is known that the endophytic fungi Culvularia lunata produce an active compound as an antibacterial that is phenol compound. Endophytic fungi Diaporthe phaseolorum produces three types of active compounds as antibacterial namely phenol, terpenoids, and alkaloids, each component of the active compound are tested for sensitivity to bacterias Escherichia coli, Staphylococcus aureus, Shigella dysenteriae, and Salmonella typhi. MIC values ??of terpenoid compounds with concentrations of 125-250 ppm are antibacterial compounds with good activity against Escherichia coli, Staphylococcus aureus, Shigella dysenteriae, and Salmonella typhi.