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Polymorphism identification of Diacylglycerol Acyltransferase1 gene and its correlation with fat content of Saanen goat Darmoatmodjo, L. M. Y. D.; Widodo, W.; Asmara, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.566 KB) | DOI: 10.14710/jitaa.43.3.211-217

Abstract

Gene markers are often usefull for selection process of cattle on commercial certain phenotypes. Saanen goat has predominance in milk production traits. Milk fat content is one of the commercial traits of milk production. Diacylglycerol Acyltransferase1 (DGAT1) gene was involved in the final process of triglyceride synthesis and associated with milk fat content. Single nucleotide subtitution or insertion-deletion in certain sequence of DGAT1 gene could be gene markers for milk fat content. The objective of this study was to identify differences variation in milk fat content among individual Saanen goats, to identify exon 14-16 polymorphism of DGAT1 gene and to examine its correlation between polymorphism and milk fat content on Saanen goats. The methodologies was collection blood and milk samples, DNA isolation from blood, amplification of DGAT1 genes, sequencing, detection of polymorphism of exon 14-16 DGAT1 gene with Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP), and examination milk quality on three lactation months using Lactoscan. The average of daily milk production from 70 Saanen goat was 2340±709 mL, 1880±632 mL, 1432±536 mL and average of milk fat content was 3.07±0.52%, 2.53±0.55%, 3.00±0.55% for the first, second and third months of lactation, respectively. The amplicon size of exon 14-16 of DGAT1 gene was ± 350 bp and showed monomorphic pattern from PCR-SSCP. Variation of milk fat content on Saanen goats not caused by polymorphism of exon 14-16 DGAT1 gene.
THE DEVELOPMENT OF GUIDED INQUIRY SCIENCE LEARNING MATERIALS TO IMPROVE SCIENCE LITERACY SKILL OF PROSPECTIVE MI TEACHERS Putra, M. I. S.; Widodo, W.; Jatmiko, B.
Jurnal Pendidikan IPA Indonesia Vol 5, No 1 (2016): April 2016
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v5i1.5794

Abstract

This study aimed to produce valid, practical and effective guided inquiry model science learning materials to enhance science literacy skill of prospective MI teachers. The tryout of the materials was implementedto students of MI teacher educationof Unipdu Jombang at academic year of 2015/2016 semesters 3 using One Group Pretest Posttest Design. The data collections were done using observation, testing, and questionnaires. Data were analysed using descriptive analysis of quantitative, qualitative and non-parametric statistical tests. The findings of the research were: 1) the learning materials were valid; 2) Practicality of the materials was tested through the implementation of lesson plans, while the learners’ activity wereappropriate to the guided inquirymodel; and 3) The effectiveness of the learning materials in terms of improvement of learning outcomes of students was seen from the n-gain with high category and increasing mastery of science literacy skills of learners also scored n-gain with high category and the response of students to the device and the implementation of learning is very positive. It was concluded that the materials were valid, practical, and effective to enhance science literacy skills of prospective MI teachers.
The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island Hartatik, Tety; Volkandari, Slamet Diah; Sumadi, S.; Widodo, W.
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.889 KB) | DOI: 10.22146/ijbiotech.7869

Abstract

The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle. Key words: Genetic diversity, Madura cattle, haplotype.
Detection and identifcation of adherence genes of intestinal-origin Lactobacillus and Pediococcus strains grown on gastric mucin in vitro Widodo, W.; Lestari, Sri; Asmara, Widya
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (858.388 KB) | DOI: 10.22146/ijbiotech.24346

Abstract

One of the primary selection criteria for potential probiotics is the ability to adhere to the host gastrointestinal tract. This study evaluated the in vitro adhesion ability on gastric mucin of two Lactobacillus casei strains (AP and AG) and two Pediococcus acidilactici strains (BE and BK), and identifed the corresponding genes responsible for adherence. Adhesion assays were performed in 96-well polystyrene microtiter plates using gastric mucin from porcine stomach as the matrix. An in vitro study on gastric mucin revealed that lactobacilli had a greater adherence ability compared with pediococci strains. The potential adherence genes were investigated using polymerase chain reaction (PCR) technology. Using specifc primers, PCR studies amplifed 150 base pairs of a potential mub gene and 161 base pairs of a potential ef-Tu gene, but no amplifed bands for potential map and bac genes were obtained. Sequence comparisons showed that the 150 and 161 amplifed base pairs were respectively homologous to the mub of Pediococcus acidilactici and ef-Tu genes of Lactobacillus paracasei. We concluded that the adherence ability of two strains of Lactobacillus casei (AP and AG) and two strains of Pediococcus acidilactici (BE and BK) on gastric mucin is in accordance with the presence of ef-Tu and mub genes. High level attachment in lactobacilli is likely to correlate with the ef-Tu gene, which is a lactobacilli-specifc adhesive gene.
Identification of Pediococcus Strains Isolated from Feces of Indonesian Infants With in vitro Capability to Consume Prebiotic Inulin and to Adhere on Mucus Widodo, W.; Anindita, Nosa Septiana; Taufiq, Tiyas Tono; Wahyuningsih, Tutik Dwi
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.164 KB) | DOI: 10.22146/ijbiotech.7859

Abstract

The aim of this experiment was to identify isolates obtained from feces of Indonesian infants and to evaluate their capability as probiotics. Identification of isolates was carried out based on morphology, physiology and biochemical identifications, and molecular identification based on 16S rRNA sequence. Morphological and physiological identification was carried out based on Gram staining, shape, motility, spore formation and catalase production. Biochemical identifications based on production of CO2 and NH3 from glucose, the ability to grow on different temperature (10 and 45°C) and pH (4.4 and 9.6), and different salt concentration (6.5 and 18%). Probiotics capability of isolates was assayed on the ability to grow on low pH (pH 2.0), on different bile salts concentration (0.3; 0.5; 1.0 and 1.5%), the capacity to grow on media with inulin as the only carbon source, and in vitro adhesion ability on porcine mucin. Morphological, physiological and biochemical identification suggest that all of isolates belong to lactic acid bacteria. Further molecular identification of five isolates showedthat isolates AA, BE and BK were strains of Pediococcus acidilactici (similarity 99%), while isolate AP and AG were strains of Lactobacillus casei (similarity 99-100%). Probiotic assays showed that more than 80% of cells of Pediococcus acidilactici isolates AA, BE and BK were viable after grown on pH 2.0 for 90 min, and around 80% of cells from the same isolates were survived on media supplemented with bile salt 1.5% for 2 h. All of isolates had high adhesion capacity as seen by more than 75% of cells attached on pig gastric mucin. Investigationof isolates to grow on inulin showed Pediococcus acidilactici isolate BE was able to consume inulin as the only carbon source. It is concluded that Pediococcus acidilactici isolate BE was a candidate probiotics and subject to further in vivo evaluation using animal models to examine their beneficial health effects.Key word : Pediococcus acidilactici, Lactobacillus casei, human origin and probiotics.
Human Origin Lactobacillus casei Isolated from Indonesian Infants Demonstrating Potential Characteristics as Probiotics in vitro Widodo, W.; Taufiq, Tiyas Tono; Aryati, Ety; Kurniawati, Asih; Asmara, Widya
Indonesian Journal of Biotechnology Vol 17, No 1 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.461 KB) | DOI: 10.22146/ijbiotech.7852

Abstract

The aim of this experiment was to isolate and identify Lactic Acid Bacteria (LAB) from infant faecesand subsequent evaluation of its potential probiotics. LAB was isolated from faeces of infants who consumedbreast milk as the only source of diet on L-cysteine-supplemented MRS Agar, and incubated on 37oC for 48hours. Colonies grew on this media were then identifi ed based on morphological, physiological and molecularapproaches. Morphological and physiological identifi cations based on Gram staining, shape, motility, sporeformation, catalase, CO2 and NH3 production, and the ability to grow on temperature at 10oC and 45oC.Molecular identifi cation based on the amplifi cation of 16S rRNA gene. The potential application of selectedisolates for probiotics was evaluated based on the ability to grow on media with low pH and the additionof 0.5% bile salts, the ability to inhibit the growth of pathogenic Bacillus cereus and Eschericia coli, and in vitroadherence ability. On the basis of morphological, physiological and molecular analysis of 16S rRNA gene, itwas concluded that the selected isolate 1AF was a strain of Lactobacillus casei. Evaluation of probiotic in vitro showed that 60.4% of cells were resistant to pH 2.0 for 90 minutes. Survival of isolate 1AF after growing at0.5% bile salts was 70.8%. The selected isolate 1AF showed the ability to inhibit the growth of Eschericia coli and Bacillus cereus with inhibitory zone of 12.00±1,00 and 15.33±1.53 mm, respectively. In vitro study on theadherence value of isolate to solid plate was found at 46.5%. It is concluded that Lactobacillus casei isolate 1AFis a potential candidate as probiotics and subject to further in vivo evaluation.
THE DEVELOPMENT OF GUIDED INQUIRY SCIENCE LEARNING MATERIALS TO IMPROVE SCIENCE LITERACY SKILL OF PROSPECTIVE MI TEACHERS Putra, M. I. S.; Widodo, W.; Jatmiko, B.
Jurnal Pendidikan IPA Indonesia Vol 5, No 1 (2016): April 2016
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v5i1.5794

Abstract

This study aimed to produce valid, practical and effective guided inquiry model science learning materials to enhance science literacy skill of prospective MI teachers. The tryout of the materials was implementedto students of MI teacher educationof Unipdu Jombang at academic year of 2015/2016 semesters 3 using One Group Pretest Posttest Design. The data collections were done using observation, testing, and questionnaires. Data were analysed using descriptive analysis of quantitative, qualitative and non-parametric statistical tests. The findings of the research were: 1) the learning materials were valid; 2) Practicality of the materials was tested through the implementation of lesson plans, while the learners activity wereappropriate to the guided inquirymodel; and 3) The effectiveness of the learning materials in terms of improvement of learning outcomes of students was seen from the n-gain with high category and increasing mastery of science literacy skills of learners also scored n-gain with high category and the response of students to the device and the implementation of learning is very positive. It was concluded that the materials were valid, practical, and effective to enhance science literacy skills of prospective MI teachers.
Perkembangbiakan Perkici Pelangi (Trichoglossus haematodus) di Laboratorium Penangkaran Widodo, W.
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.175

Abstract

The research was conducted to understand the breeding aspects of the Rainbow Lorikeet (Trichoglossus haematodus), which were caged in flocks and as a pair, in two aviaries of the Animal House Laboratory of Zoological Division, the Research Center of Biology, Indonesian Institute of Sciences (LIPI), Cibinong. From January 2002 until January 2005, 11 birds of the Rainbow Lorikeets were used in this research,. The birds were divided into two groups, the first group (9 individuals)  were caged in the first aviary  and the second one (2 individuals) were caged in the second aviary. The  size of each aviary room (LxWxH) was 3.32 m x 1.82 m x 2.85 m. The aviary rooms were equiped with the roosting, nest boxes, feeding and drinking water places. Foods and water were given in cafetaria everyday, starting from about 08.00 a.m. The foods consisted of bird food/”pur” (150 grams), white bread (15 grams), cooked quail eggs (60-70 grams), the lampung bananas (300 grams), slices of corn (100 grams), bean sprouts (50 grams), and palm sugar (50 grams). All of the igredients were mixed with 450-500 ml water and were then pulverized. The birds were also given some vegetables. The results were as follows: the Rainbow Lorikeets caged in flocks have produced two chicks and those in a pair only produced one chick. The clutchs consisted of  1-2 eggs. Eggs were white, smooth and their shape ovale. Average weight of the eggs was 7-8 grams. Weight of day old chicks was about 8 grams, those chicks were still blind and with out featheres (“altricial”). Incubation periods was 25-26 days, and incubated either by the female or the male. Fledging period was 71 days or more. The growing of the featheres and young birds of the Rainbow Lorikeets will be also discussed in this paper.
Populasi dan Pola Sebaran Burung di Hutan Wanawisata Galunggung, Tasikmalaya, Jawa Barat Widodo, W.
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2932

Abstract

Gunung Galunggung ketika erupsi tahun 1982 sebagian besar flora dan fauna yang ada disekitarnya luluh lantak. Penelitian bertujuan mengetahui populasi dan pola sebaran burung-burung yang terdapat di kawasan Galunggung periode pemulihan pasca 31 tahun meletus.Metode point count jarak tidak tetap digunakan dalam penelitian ini Gunung Galunggung telah ditetapkan sebagai kawasan Wanawisata Galunggung sejak tahun. Penelitian dilakukan dengan membagi dalam 5 blok pengamatan. Sejumlah 80 titik penghitungan burung ditetapkan dalam 0,7222 km2 luasan area. Berdasarkan hasil penelitian dijumpai 39 spesies burung dengan total 719 individu. Ada 10 spesies burung ditemukan dengan populasi tinggi, yaitu Cynniris jugularis (96,93 ind/km2), Lonchura leucogastroides (84,46 ind/km2), Orthotomus sutorius (70,61 ind/km2), Orthotomus cucullatus (62,30 ind/km2), Zosterops palpebrosus (67,85 ind/km2), Pycnonotus aurigaster (55,39 ind/km2), Brachypteryx leucophrys (41,54 ind/km2), Stachyris melanothorax (38,77 ind/km2), Streptopelia chinensis (30,46 ind/km2), dan Halcyon cyanoventris (26,30 ind/km2). Nilai indeks Morista sebesar 7,67, ini menunjukkan bahwa pola sebaran dari sebagian besar burung di Wanawisata Galunggung adalah mengelompok. Secara spesifik tercatat 1 spesies burung sebaran terbatas (Stachyris melanothorax), 1 spesies burung migran (Motacilla cinerea), dan beberapa spesies burung endemik dan dilindungi.When the Galunggung mountain erupted in 1982, most of Galunggungs flora and fauna were devastated severely. A research has been carried out to know about bird population and their dispersion patterns in the Galunggung Tourism Forest after 31 years of restoration phase. The research used nonfixed distance Point Count method and this research was conducted by dividing the observation area into 5 blocks. Eighty watchout points have been established in an area of 0.7222 km2. The study revealed 39 bird species with total of 719 individuals. Among them, 10 species had relatively high population, i.e. Cynniris jugularis (96.93 inds/km2), Lonchura leucogastroides (84.46 inds/km2), Orthotomus sutorius (70.61 inds/km2), Orthotomus cucullatus (62.30 inds/km2), Zosterops palpebrosus (67.85 inds/km2), Pycnonotus aurigaster (55.39 inds/km2), Brachypteryx leucophrys (41.54 inds/km2), Stachyris melanothorax (38.77 inds/km2), Streptopelia chinensis (30.46 inds/km2), and Halcyon cyanoventris (26.30 inds/km2). The Morisita index was 7.67, which indicates that some bird species flocked in groups. The range of Stachyris melanothorax was restricted, whereas Motacillacinerea is considered as migrant species, and others were endemics and protected birds.
Teachers’ Performance in Science Learning Management Integrated with Character Education Isdaryanti, B.; Rahman, M.; Sukestiyarno, Y. L.; Florentinus, T. S.; Widodo, W.
Jurnal Pendidikan IPA Indonesia Vol 7, No 1 (2018): March 2018
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v7i1.12887

Abstract

The research aims at revealing the performance of teachers in science learning management integrated with character education. This study employed qualitative research method. Based on the result of the t-test of the correlation coefficient, this study obtained t value 4,210 with significance 0,001. The significance value 0,001 < 0,05 showed that there is influence from teachers’ performance in arranging learning media integrated with character education to the teachers’ performance in science learning. The conclusion of research stated that the performance of science teachers in junior high schools in Semarang City in integrating character education is categorized into a very good category with average score 85,05.