Articles

INHIBITION OF LIPID PEROXIDATION INDUCED BY ULTRAVIOLET RADIATION BY CRUDE PHLOROTANNIS ISOLATED FROM BROWN ALGAE SARGASSUM HYSTRIX V. BUXIFOLIUM C. AGARDH Harnita, Agnes Nora Iska; Santosa, Ign. Edi; Martono, Sudibyo; Sudarsono, Sudarsono; Widyarini, Sitarina; Harren, Frans J.M.
Indonesian Journal of Chemistry Vol 13, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2295.873 KB) | DOI: 10.22146/ijc.21320

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This study examines the antioxidant activity of crude phlorotannins from the brown algae Sargassum hystrix v. buxifolium (Chauvin) J. Agardh, through the inhibition of a lipid peroxidation reaction that is induced by the UV radiation. The antioxidant activity during the UV exposure was investigated using the laser-based photoacoustic method for the detection of the ethylene as indicator for lipid peroxidation. This involves an experiment that isolated crude phlorotannins from the ethyl acetate fraction of the Sargassum hystrix methanol extract, hereafter referred to as PFSH. It results in the antioxidant activity as a potent lipid peroxidation inhibitor. Statistically, such antioxidant activity is not significantly different than the commercial antioxidant, which is vitamin C (p > 0.05). The amount of the total phlorotannins, using the Folin-Ciocalteu method, was measured to be approximately 0.13% w/w. In addition, it is found that PFSH contains phlorotannins with low molecular weight (MW) (
Dampak Induksi Karsinogenesis Glandula Mammae dengan 7, 12-dimetilbenz(?)antrasen terhadap Gambaran Histopatologis Lambung Tikus Sprague Dawley Tri Budi M, Ranita; Widyarini, Sitarina
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was designed to study the impact of mammary carcinogenesis induced by 7,12-dimetilbenz (?) antracene (DMBA) on histopathological features of the gastric in Sprague Dawley rat.Eight female Sprague Dawley (SD) rats, of four week-old, were used in this study. The rats were randomlydivided into 2 groups of four. Group I were treated orally with 20 mg/kg BW of 7,12-dimetilbenz (?)antracene (DMBA) treatment, twice a week for 5 weeks. Group II were used as negative control withoutDMBA treatment. At 5 week after the end DMBA of, the rats of the both groups were sacrificed and thegastric were fixed in 10% buffer formalin. Subsequently, tissue sections were stained with hematoxylinand eosin (H&E) for microscopic examination. The results of the experiment show that oral administrationof DMBA (20 mg/kg BW) twice a week, for 5 weeks result in epithelial hyperplasia of cardia. Hemorrhagicand inflammation reactions are also found on the mucous membrane of fundic. However, macroscopicexamination shows no specific changes of the gastric. Histopathological changes were suspected as a precancer lesion of the gastric due to chronic exposure of DMBA.
Terapi Ammonium Khlorida-Asam Askorbat untuk Menurunkan Tingkat Keasaman Urin dan Kristalisasi Struvit pada Kucing Urolithiasis (THERAPY AMMONIUM CHLORIDE-ASCORBIC ACID FOR LOWERING URINE ACIDITY AND STRUVITE CRYSTALLIZATION IN FELINE UROLITHIASIS) Nururrozi, Alfarisa; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Purnamaningsih, Hary; Widyarini, Sitarina; Raharjo, Slamet; Ramandani, Dhasia
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.516 KB) | DOI: 10.19087/jveteriner.2019.20.1.8

Abstract

Struvite/Magnesium ammonium phosphate (MAP) is common causes of feline urolithiasis. Prevent the formation of MAP crystallization can be treated by adjusting the pH urine in acid conditions. Urine with an acid pH will decrease struvite activity product (SAP) by preventing the phosphate deprotonation process which is the main constituent of struvite components. This study aims to determine the potential of a combination of ammonium chloride (NH4Cl) and ascorbic acid to decrease SAP by retain the urine pH under normal conditions. Twelve male cats, 2-5 years old and body weight 3.0 ± 0.8 kg were diagnosed struvite urolithiasis used for this study. Cats have a9clinical history of hematuria, dysuria, polyuria, and stranguria. Diagnosis of urolithiasis performed by clinical examination, USG, x-rays, and urinalysis. Cats that found struvite urolith, treated with 200 mg/kg NH4Cl and 100 mg/kg ascorbic acid orally twice a day. The research objects were observed included urine pH and crystaluria density. The data were analyzed using analisis of varian. The result of this study showed NH4Cl and ascorbic acid treatment can decrease the urine pH at 8 cats (89%) with an average pH 6.3 ± 0.3. Microscopic examination of the urine showed the struvite crystallization more infrequently than before therapy. The study concluded that the combination of NH4Cl and asam askorbat can lower the urine pH and reduce struvite crystal density in the urine.
HYPERLIPIDEMIA PREVENTING ACTIVITIES OF STANDARDIZED ETHANOLIC EXTRACT OF RED SPINACH (AMARANTHUS TRICOLOR L.): AN IN VIVO STUDY IN MALE SPRAGUE-DAWLEY RATS Pradana, Dimas Adhi; Apriani, Lalily; Widyarini, Sitarina
Jurnal Kefarmasian Indonesia VOLUME 8, NOMOR 2, AGUSTUS 2018
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.559 KB) | DOI: 10.22435/jki.v8i2.349

Abstract

Lipid metabolism disorders can lead to hyperlipidemia that triggers atherosclerosis. This study aimed to identify the potential of standardized ethanolic extract of red spinach (Amaranthus tricolor L.) to prevent hyperlipidemia by referring to the reduction of triglyceride level and total cholesterols in male Sprague-Dawley rats. A total of 30 experimental animals was put into 6 groups, including normal, positive control (0.9 mg/kgBW of simvastatin), negative control, treatment I (200 mg/kgBW of extract), treatment II (400 mg/kgBW of extract), and treatment III (800 mg/kgBW of extract). Preventive therapy and positive control were administered from day 1 to day 67. Hyperlipidemia was induced by feeding pure lard and duck yolk to the rats twice daily from day 8 to day 67. Determination of triglyceride level and total cholesterols was conducted on day 0 and day 67. The findings revealed that the treatment groups with ethanolic extract of red spinach at doses of 200 mg/kgBW, 400 mg/kgBW, and 800 mg/kgBW had statistically significant differences (p
EFEKTIVITAS PENTAGAMAVUNON-0 TERHADAP PENGHAMBATAN EKSPRESI SIKLOOKSIGENASE-2 PADA MODEL KANKER KOLON TIKUS Yulianty, Risfah; Hakim, Lukman; S, Sardjiman; Alam, Gemini; Nufika, Riska; Widyarini, Sitarina
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.344

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Penelitian ini bertujuan menentukan efektivitas pentagamavunon-0 (PGV-0) terhadap penghambatan ekspresi siklooksigenase-2 (COX-2) pada kanker kolon tikus Wistar. Pada penelitian ini digunakan 20 ekor tikus Wistar jantan yang dibagi secara acak dalam 4 kelompok perlakuan. Kelompok I merupakan kontrol negatif, kelompok II kontrol positif, kelompok III diberi PGV-0 40 mg/kg BB selama 15 minggu, dan kelompok IV diberi PGV-0 40 mg/kg BB selama 25 minggu. Pemberian PGV-0 dilakukan secara oral dua kali seminggu. Induksi kanker kolon dilakukan dengan cara injeksi subkutan DMH 60 mg/kg BB, satu kali seminggu selama 15 minggu. Pada minggu ke-26, semua hewan coba dieutanasia, kolon difiksasi dalam formalin 10% untuk selanjutnya diamati perubahan makroskopik dan mikroskopik. Penilaian ekspresi COX-2 dilakukan dengan menggunakan metode Duke’s stage dan skor imunoreaktivitas (IRS). Hasil penelitian ini memperlihatkan pemberian PGV-0 selama 25 minggu menurunkan jumlah nodul kanker kolon dari 5 ke 2 (berkurang 60%); diameter kanker kolon (pxl) dari 0,712 mm² ke 0,0043 mm² (berkurang 99,31%). Pemberian PGV-0 selama 15 minggu hanya menurunkan jumlah nodul 10% dan area kanker kolon dari 0,712 mm² ke 0,0062 mm² (99,07%). Skor imunoreaktivitas COX-2 diekspresikan oleh kelompok III dan IV adalah 4 dan 5. Gambaran histologis dari kolon mendukung hasil di atas. Pemberian PGV-0 efektif menurunkan jumlah dan area nodul kanker kolon melalui penghambatan ekspresi COX-2.
ISOPROTERENOL SEBAGAI MODEL INFARK MIOKARDIAL AKUT PADA TIKUS WISTAR Widiyaningsih, Wahyu; Sugiyanto, Sugiyanto; Widyarini, Sitarina; Pramono, Suwidjiyo
Jurnal Ilmu Farmasi dan Farmasi Klinik Prosiding Seminar Nasional "Peluang Herbal Sebagai Alternative Medicine"
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/jiffk.v0i0.1339

Abstract

ABSTRACT Decreased oxygen supply and necrosis in the heart muscle cells can lead to acute myocardial infarction. Free radical compounds can lead to oxidative stress in the heart muscle and lead to myocardial infarction. Isoproterenol is a non-selective ?-adrenergic agonist can lead to acute myocardial infarction in large doses. The purpose of this study was to validate the model of acute myocardial infarction. This study using male Wistar rats aged two months as the test animal. Male Wistar rats divided into two groups: control group and treatment group. The control group treated with physiological saline and the treatment group treated with isoproterenol 85 mg/kg BW. Isoproterenol administered subcutaneously for two doses with an interval of 24 hours. After 48 hours of the isoproterenol first administration, rat heart rhythm's records with an electrocardiogram (ECG). Furthermore, the rats sacrificed and performed a necropsy. The left ventricle part in the cross section for the macroscopic observation (extensive infarction) with triphenyl tetrazolium chloride (TTC) staining, microscopic observation of infarction with hematoxylin-eosin staining (HE) and immunohistochemical (IHC) to observe the expression of caspase-3. The ECG of isoproterenol group showed elevation in the ST segment. TTC staining shows the expansion of the infarct area. The HE staining showed the typical image of myocardial infarction and the IHC staining results showed an increase in the expression of caspase-3. The results showed that isoproterenol can be use as a model myocardial infarction with parameters of infarction are ECG changes, TTC staining of infarct broad macroscopic image, a histopathological image of heart and apoptosis with the expression of caspase-3. Key words: Isoproterenol, acute myocardial infarction, cardiac histopathology
PENENTUAN AKTIVITAS ISOLAT ANDROGRAFOLID TERHADAP α-AMILASE DAN α-GLUKOSIDASE MENGGUNAKAN METODE APOSTOLIDIS DAN MAYUR Rais, Ichwan Ridwan; Nugroho, Agung Endro; Samudra, Agung Giri; Widyarini, Sitarina
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.049 KB) | DOI: 10.14499/mot-TradMedJ18iss3pp162-166

Abstract

Gangguan metabolisme karbohidrat dapat menyebabkan Diabetes mellitus. Karbohidrat dalam saluran cerna mengalami metabolisme menjadi glukosa yang sederhana kemudian diabsorbsi masuk kedalam peredaran darah serta mempengaruhi kadar glukosa dalam darah. Proses penyerapan ini dikatalisis enzim pemecah ikatan α-1,4-glikosida yaitu α-amilase dan enzim pemecah ikatan α-1,6-glikosida yaitu α-glukosidase yang terdapat pada sel usus. Telah dilakukan penelitian sebagai upaya mengembangkan pengobatan alternatif diabetes mellitus dengan menguji kemampuan isolat andrografolid dalam menghambat aktivitas α-amilase dan α-glukosidase secara in vitro. Isolat andrografolid memperlihatkan aktivitas yang cukup baik dalam menghambat α-amilase (IC50= 12,49 mg/mL) dan lemah dalam menghambat α-glukosidase (IC50= 38,86 mg/mL). Penghambatan aktivitas α-amilase ini menjadi bukti salah satu mekanisme andrografolid dalam mengurangi metabolisme karbohidrat yang dapat mempengaruhi kadar glukosa dalam darah dan mengindikasikan andrografolid sebagai obat alternatif yang cukup potensial dalam mengatasi penyakit diabetes mellitus.
Immunodiagnosis Infeksi Aeromonas hydrophila pada Ikan Kristianingrum, Yuli Purwandari; Widyarini, Sitarina; Kurniasih, Kurniasih; Sutrisno, Bambang; Tabbu, Charles Rangga; Sugiyono, Sugiyono
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8456.913 KB) | DOI: 10.22146/jsv.38858

Abstract

Aeromonas hydrophila causes a disease that often infects fish and is known as Motile Aeromonas Septicaemia (MAS), Hemorrhagi Septisemia, Ulcer disease or Red-Sore disease. The   aims of this study were to develop polyclonal antibody of  Aeromonas hydrophila in the rabbits to   confirm the diagnosis of Aeromonas hydrophila  in the fish by immunohistochemistry staining method. Preparation of polyclonal antibodies was performed on the rabbits used to Aeromonas hydrophila bacteria that have been tested biochemically by intravenous and intraperitoneal injection. Doses of Aeromonas hydrophila  bacteria were 109 CPU/ml  of 0.5 ml at first injection, 1 ml at second injection, 2 ml at thirth injection and 3 ml at fourth injection. Blood serum collection was performed at week 5 after injection from  an  ear and intracardial vein. The result of antibody titer was 28 = 1024 which measured by   tube test. Furthermore, polyclonal   antibody was used to immunohistochemistry  staining with 400x dilution. The results of the staining showed that an immunopositive reaction in the liver, skin,lien,  gill, kidney, and heart of fish to Aeromonas hydrophila antibody. The research conclution was polyclonal antibody from rabbit can be used to accurately confirm the diagnosis of Aeromonas hydrophila  based on antigen and antibody reaction. 
Kepadatan Sel Hipokampus Insulin Imunoreaktif pada Formasi Hipokampus Mencit yang Diinduksi Berulang dengan Streptozotosin (THE DENSITY OF HIPPOCAMPUS INSULIN IMMUNOREACTIVE CELLS IN HIPPOCAMPUS FORMATION OF REPEAT STREPTOZOSIN INDUCED MICE) ., Erwin; Pangestiningsih, Tri Wahyu; Widyarini, Sitarina
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The presence of insulin in the hippocampus may indicate its involvement in brain cognitive function,such as learning and memory phenomena. The purpose of this study was to find out the density ofhippocampus insulin immunoreactive cells in hippocampus formation in Balb-C mice which treated withstreptozosin repeated as the animal model of diabetes mellitus. Thirty male mice Balb-C strain, aged 12-14 weeks, weight 30-40 g, divided into 2 treatment groups, each group consisted of 15 individuals. GroupI (KI) was treated with sodium citrate buffer, while group II (K2) was treated with streptozotosin at  dose0,5 ml of 40 mg/kg bw in 50 mM sodium citrate buffer pH 4.5 in intra-peritoneal of for five consecutive days.Every two animals from each group euthanasia and necropsied on day 7, 14, 21 and 28 respectively afterthe administration of treatment. Subsequently, the brain tissues were collected and fixatived in NBF10%. Brain sampel were the processed immunohistochemically using anti-insulin mouse antibody. Thedensity of hippocampus insulin immunoreactive cells in hippocampus formation in group 1 were highercompared to group 2. This comparasion as well as the time of observation and interaction between groupand time showed significant differences (p<0.05). it can be concluded that low-dose induction of repeatedstreptozotosin may  cause a decrease in density of hippocampus insulin imunoreaktif cell.
PENGARUH PEMBERIAN KOMBINASI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP BERAT BADAN DAN JUMLAN NODUL TIKUS SPRAGUE DAWLEY BETINA YANG DIINDUKSI 7,12 DIMETILBENZ(α) ANTRASEN (DMBA) Nurani, Laela Hayu; Mursyidi, Achmad; Widyarini, Sitarina
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.079 KB) | DOI: 10.12928/pharmaciana.v6i1.3093

Abstract

Pasak Bumi roots contain quasinoid compounds that have carcinogenesis inhibitory, imunomudulator, antiulcer, and antimalaria effects. This study aims to determine the effect of a combination of ethyl acetate fraction of Pasak Bumi roots and doxorubicin on DMBA-induced white mice by weight and palpation observation. The mice were divided into 8 groups. Each group consisted of 7 female Sprague Dawley mice. Group 1 (normal) was only given food and drink, Group 2 was given fraction of ethyl acetate of Pasak Bumi roots (100 mg/kg body weight, Group 3 (positive control) was given doxorubicin (1.17 mg/kg body weight) 1 times a week for 5 weeks, Group 4 was given DMBA (20 mg/kg) two times a week for 5 weeks, Groups 5 was given DMBA (20 mg/kg) + doxorubicin (1.17 mg/kg), Group 6 was given DMBA (20 mg / kg) + ethyl acetate fractions of Pasak Bumi roots (100 mg / kg), Group 7 was given DMBA (20 mg / kg) + doxorubicin (1.17 mg / kg) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg) and Group 8 doxorubicin ( 1.17 mg / kg body weight) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg). Observations were made on the basis of body weight of mice and tumor incidence by palpation at week 6 of treatment. The result of  this research shows that the administration of ethyl acetate fraction of Pasak Bumi roots and doxorubicin combination can increase body weight of the mice and decrease the number of tumors compared to single doxorubicin.
Co-Authors Abdul Rohman Achmad Mursyidi Adhi Pradana, Dimas Adrenalin, Sruti Listra Agnes Nora Iska Harnita Agung Endro Nugroho Agung Giri Samudra, Agung Giri Agustina Dwi Wijayanti Agustinus Yuswanto, Agustinus Ahmad Mursyidi Alfarisa Nururrozi, Alfarisa Ana Sahara Anggraini, Neni Tri Apriani, Lalily Asmarani Kusumawati Awik Puji Dyah Nurhayati Azzahra, Fara Balfas, Rifqi Ferry Bambang Sutrisno Charles Rangga Tabbu Devi Usdiana Rosyidah, Devi Usdiana Devita Anggraeni Dewajani Purnomosari, Dewajani Dewi Hidayati Dhasia Ramandani, Dhasia Dhirgo Adji Dimas Adhi Pradana, Dimas Adhi Dwi Priyowidodo Dwiratna, Deasy Wulan Edy Meiyanto Eka Kumalasari Endang Dwi Wulansari Erwin . Erwin E Eryl Sri Rohayati Eti Nurwening Sholikhah Etriwati E Farida Hayati Fauziah, Ima Frans J.M. Harren Gani, Andayana Puspitasari Gemini Alam Haribowo, Nurman Harjadi, . Harjadi, Harjadi Hary Purnamaningsih Hastari Wuryastuty Helminawati, Helminawati Ichwan Ridwan Rais Ign. Edi Santosa Imanjati, Lynda Nugrahaning Indwiani Astuti Irianiwati Irianiwati Irianiwati, . Isnindar Isnindar, Isnindar Iwan Sahrial Hamid Jainudin, Hizriah Alief Joko Prastowo Jumina Jumina Kintoko Kintoko Kurniasih . Kurniasih Kurniasih Laela Hayu Nurani Lukman Hakim Marchaban Marchaban, Marchaban Michael Haryadi Wibowo Mulyati, Galih Dwi Mustofa Mustofa Muttaqien M Nemay Anggadewi Ndaong, Nemay Anggadewi Ngatidjan Ngatidjan Nurani, Laela Hayu Nurani, Laela Hayu Nurwijayanti, Anandita Padauleng, Novrita Padauleng, Novrita Prakasita3, Vinsa Cantya Puspa Wikan Sari R Wasito R. Wasito Ranita Tri Budi M Rarastoeti Prastiwi Riana, Fajar Slamet Risfah Yulianty Riska Nufika Ruslin Hadanu Rusmihayati, Rusmihayati Saputri, Lintang Cahya Sardjiman S Slamet Raharjo Soedarmanto Indarjulianto Sri Herwiyanti, Sri Subagus Wahyuono SUDARSONO SUDARSONO Sudibyo Martono Sugiyanto . Sugiyanto Sugiyanto Sugiyono Sugiyono Sukardiman Sukardiman Suwidjiyo Pramono Tri Untari Tri Wahyu Pangestiningsih Tria Zakinah, Tria Ustrina, Nura Vembriarto Jati Pramono, Vembriarto Jati Wahyu Widiyaningsih, Wahyu Wahyuni, Agnesia Endang Trihastuti Wijayanti Pujitono Yanuartono, Yanuartono Yuli Purwandari Kristianingrum, Yuli Purwandari Yustina Sri Hartini, Yustina Sri Yusuf, Siti Salma Yuswanto Yuswanto