Nyoman Suci Widyastiti
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PENGARUH SUPLEMENTASI VITAMIN E (α-TOKOFEROL) TERHADAP KADAR GAMMA GLUTAMIL TRANSFERASE (GGT) DAN KADAR NITRIC OXIDE (NO) PADA TIKUS (STUDI PADA TIKUS RATTUS NOVERGICUS STRAIN WISTAR JANTAN TERPAPAR INHALASI UAP BENZENE) Roziana, Roziana; Subagio, Hertanto Wahyu; Suhartono, Suhartono; Widyastiti, Nyoman Suci
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 3, No 2 (2015)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.835 KB) | DOI: 10.14710/jgi.3.2.73-79

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Latar Belakang : Paparan benzene dapat menyebabkan stres oksidatif, diantaranya ditandai dengan peningkatan kadar GGT dan kadar NO. Vitamin E dapat mengurangi dan mencegah produksi radikal bebas. Reaktivitas hidrogen fenolik pada kelompok hidroksil ?-tokoferol akan menstabilkan elektron tidak berpasangan pada radikal bebas.Tujuan : Membuktikan pengaruh suplementasi vitamin E terhadap kadar GGT dan kadar NO pada tikus jantan terpapar uap benzene.Metode : Penelitian ini adalah penelitian eksperimen pada binatang coba dengan rancangan post test only controlled group design. Sampel 35 ekor tikus strain wistar jantan dibagi secara random dalam enam kelompok yaitu kontrol negatif, kontrol positif dan empat kelompok perlakuan suplementasi vitamin E dosis bertingkat (X1 = 1,8 IU;  X2 = 3,6 IU; X3 = 7,2 IU; dan X4 = 14,4 IU per hari untuk setiap 200 gram berat badan tikus). Setiap kelompok kecuali kontrol negatif dipapar 300 ppm uap benzene selama 2 minggu (6 hari/minggu, 6 jam/hari). Pengukuran kadar GGT menggunakan metoda Kinetik Colorimetric dan kadar NO menggunakan metoda Colorimetric Gries dilakukan pada setiap kelompok. Hasil : Suplementasi vitamin E berpengaruh menurunkan  kadar GGT (p=0,0001). Pengaruh paparan benzene terhadap peningkatan kadar GGT dapat dicegah dengan baik pada dosis suplementasi vitamin E 14,4 IU/200gr BB tikus/hari. Suplementasi vitamin E tidak berpengaruh terhadap kadar NO.Simpulan : Ada pengaruh suplementasi vitamin E terhadap penurunan kadar GGT dan  tidak ada pengaruh suplementasi vitamin E terhadap penurunan kadar NO.
PENGARUH SUPLEMENTASI β-CAROTENE TERHADAP KADAR GLUKOSA DARAH DAN KADAR MALONDIALDEHIDA PADA TIKUS SPRAGUE DAWLEY YANG DIINDUKSI STREPTOZOTOCIN Soviana, Elida; Rachmawati, Banundari; Widyastiti, Nyoman Suci
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 2, No 2 (2014)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.768 KB) | DOI: 10.14710/jgi.2.2.41-46

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Background : Hyperglycemia on diabetes mellitus can cause increasing of free radicals production. Free radicals caused lipid peroxidation reaction by forming malondialdehyde (MDA). ?-carotene has antioxidant activity may inhibit the formation of ROS.Objective : To prove the effect of multilevel doses ?-carotene 1 mg/kg BW, 20 mg/kg BW and 20 mg/kg BW on alternate days within 30 days orally supplementation on blood glucose level and MDA level on Sprague Dawley male rats induced by streptozotocin (STZ). Methods : Thirty rats were randomly divided into 5 groups: X1=Negative control/diabetic, X2 (STZ 40 mg/kg BW + BC 1 mg/kg BW), X3 (STZ 40 mg/kg BW + BC 10mg/kg BW), X4 (STZ 40 mg/kg BW + BC 20 mg/kg BW), X5 (technic control/non diabetic). ?-Carotene supplementation was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP and MDA level by ELISA with TBARS methods. Data was analized using paired t-test, wilcoxon, one way anova and post hoc bonferroni. Results : there was a significant difference of blood glucose level (p = 0,0001) and MDA level (p = 0,0001) after suplementation ?-carotene on alternate days within 30 days orally. ?-carotene 10 mg/kg BW was the most effective and efficient dose to lowering blood glucose, while 20 mg/kg BW to lowering MDA level. Conclusion : The multilevel doses ?-carotene (1 mg/kg BW, 10 mg/kg BW and 20 mg/kg BW) on alternate days within 30 days orally supplementation can decrease blood glucose and MDA level. ?-carotene 10 mg/kg BW is the most effecetive and efficient to decrease blood glucose and ?-carotene 20 mg/kg BW to decrease MDA level.
MELATONIN MENURUNKAN BERAT BADAN TETAPI TIDAK MENURUNKAN KADAR TNF-α PADA TIKUS WISTAR JANTAN YANG DIBERI MINYAK JELANTAH SELAMA 28 HARI Novianti, Anugrah; Dharmana, Edi; Widyastiti, Nyoman Suci
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 5, No 2 (2017)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.997 KB) | DOI: 10.14710/jgi.5.2.127-132

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Backgound: Non Alcoholic Fatty Liver Disease (NAFLD) occurs when the intake and free fatty acid synthesis occurs more frequently than its oxidation and resecretion in the blood. Melatonin is a powerful antioxidant that can boost the synthesis of endogenous antioxidants in the body, suppress the inflammatory response and inhibit the formation of steatosis.Objective: To analyze the effect of melatonin supplementation in reducing body weight andTNF-? levels in male Wistar rats were fed by waste cooking oil.Methods: True experimental studyusing post-test only control group design. This study was done on 18 male wistar rats were divided into 3 groups : the positive control group (P0) was administrated waste cooking oil, the treated group 1 (P1) was administrated waste cooking oil and 5mg/kgBW melatonin, and the treated group 2 (P2) was administrated waste cooking oil and 10mg/kgBW melatonin for 28 days.Data analysis using One Way ANOVA test and followed by Tukey test to determine the most effective dose of melatonin.Results: There was significant difference in body weightbetween P2group and K0 group (p=0,019) with the mean body weight difference was 19,167g lower than K0 group. There was no difference in TNF-? levels between the three groups (p=0,155). Conclusion: Melatonin dose of 10mg/kgBWloses body weight male Wistar rats have given by waste cooking oil for 28 days, but does not reduce TNF-? levels.
PERBANDINGAN PENGARUH PEMBERIAN EKSTRAK BAWANG PUTIH (ALLIUM SATIVUM L),KITOSAN DAN YOGURT SINBIOTIK PISANG TANDUK TERHADAP PROFIL LIPID TIKUS SPRAGUE-DAWLEY HIPERKOLESTEROLEMIA Budianto, Yanuarius Alvin Pratama; Widyastiti, Nyoman Suci; Arisota, Ariosta
DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO ) Vol 7, No 2 (2018): JURNAL KEDOKTERAN DIPONEGORO
Publisher : DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO )

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Abstract

Latar Belakang : Dislipidemia merupakan abnormalitas metabolisme lipid yang ditandai dengan peningkatan maupun penurunan fraksi lipid dalam serum. Berbagai penelitian terdahulu membuktikan bawang putih (Allium sativum L), kitosan dan yogurt sinbiotik pisang tanduk mempuyai efek memperbaiki profil lipid.Tujuan : Menganalisis perbandingan pengaruh pemberian ekstrak bawang putih, kitosan dan yogurt sinbiotik pisang tanduk terhadap profil lipid tikus Sprague-Dawley hiperkolesterolemia.Metode : Penelitian eksperimental dengan disain Post Test Control Group Design. Jumlah sampel 30 tikus Sprague-Dawley jantan, terbagi dalam 5 kelompok, terdiri dari kelompok kontrol normal (K1), kontrol hiperkolesterolemia (K2)dan kelompok perlakuan P1,P2,P3 yang masing-masing diberi ekstrak bawang putih, kitosan atau yogurt sinbiotik pisang tanduk. Analisis statistik yang digunakan adalah uji One Way Anova dan Post Hoc Bonferroni.Hasil : Rerata kadar kolesterol total K1,K2,P1,P2,P3 adalah 83,67 ± 3,68; 207,79 ± 5,36; 105,11 ± 3,03; 117,64 ± 4,17; 128,35 ± 3,85 mg/dL;. Rerata kadar trigliserida K1,K2,P1,P2,P3 adalah 73,68 ± 4,20;130,59 ± 3,03; 82,22 ± 1,94; 92,33 ± 3,21; 103,51 ± 3,11mg/dL;. Rerata kadar kolesterol HDL K1,K2,P1,P2,P3  adalah 89,06 ± 1,99; 25,23 ± 1,52; 74,13 ± 3,09; 62,42 ± 2,04; 56,63 ± 2,11 mg/dL;. Rerata kadar kolesterol LDL K1,K2,P1,P2,P3 adalah 24,57 ± 2,04; 77,02 ± 1,64; 35,46 ± 2,35; 45,15 ± 1,72; 56,18 ± 1,49 mg/dL.Kesimpulan : Terdapat perbedaan pengaruh pemberian ekstrak bawang putih ,kitosan dan yogurt sinbiotik pisang tanduk terhadap profil lipid tikus Sprague-Dawley  hiperkolesterolemia.Ekstrak bawang putih menunjukkan efek paling baik dalam memperbaiki profil lipid , diikuti oleh kitosan dan yogurt sinbiotik pisang tanduk.
Vitamin E effect on osmotic fragility in β thalassemia major Fitrianto, Agus; Tamam, Moedrik; Widyastiti, Nyoman Suci
Paediatrica Indonesiana Vol 54, No 5 (2014): September 2014
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi54.5.2014.280-3

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Background Blood transfusion remains the main therapy for anemia in β thalassemia major patients. However, frequent transfusions can cause oxidative stress in response to iron overload. Vitamin E is considered to be the best lipid-soluble exogenous antioxidant in humans. It can protect phospholipid membrane from peroxidarion. Erythrocyte osmotic fragility is a useful test to assess for the improvement of red blood cells in thalassemia patients after vitamin E supplementation.Objective To investigate the effect of vitamin E for improving erythrocyte osmotic fragility in β thalassemia major and for decreasing the need for frequent transfusions.Methods T his was a double blind placebo controlled randomized clinical trial on children aged 2-14 years with thalassemia major who received frequent blood transfusions. Fifty subjects were divided into 2 groups: group I with vitamin E supplementation and group II with placebo, as a control group, for a period of 1 month. Pre- and post-treatment data on erythrocyte osmotic fragility and hemoglobin level were analyzed with non-paired T-test.Results Improved erythrocyte osmotic fragility was found: in group I, pre-treatment 31.59 (SD 6.342)% to post-treatment 38.08 (SD 7.165)%, compared to the control group pre-treatment 34.40 (SD 6.985)% to post-treatment 29.26 (SD 9.011)% (P=0.0001). Comparison of the mean delta Hb level in group  I was 0.94 (SD 0.605) gr% and that of group II was - 0.23 (SD 1.199) gr% (P=0.0001).Conclusion Vitamin E supplementation improves erythrocyte fragility and Hb level in β thalassemia major pediatric patients.
PENGARUH PEMBERIAN JAMUR KUPING HITAM (AURICULARIA POLYTRICHA) TERHADAP KADAR TRIGLISERID SERUM TIKUS WISTAR YANG DIINDUKSI MINYAK JELANTAH Yasa, I Made Miarta; Abdurachmin, Henna Rya; Widyastiti, Nyoman Suci
DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO ) Vol 6, No 2 (2017): JURNAL KEDOKTERAN DIPONEGORO
Publisher : DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO )

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Latar belakang : Pada era modern seperti sekarang ini banyak sekali orang-orang yang lebih memilih untuk makan-makanan cepat saji. Namun biasanya minyak yang digunakan untuk menggoreng makanan tersebut adalah minyak jelantah. Padahal minyak jelantah itu sendiri memiliki lemak trans yang begitu tinggi, sehingga menyebabkan naiknya kadar kolesterol salah satunya trigliserid. Hal itu dapat menyebabkan meningkatnya kejadian suatu penyakit seperti aterosklerosis. Jamur kuping hitam diketahui memiliki kandungan vitamin, polisakarida, dan zat-zat lain yang dapat menurunkan kadar trigliseridaTujuan : Mengetahui efek pemberian jamur kuping hitam terhadap penurunan kadar trigliserid pada tikus wistar jantan yang diinduksi minyak jelantahMetode : Penelitian ini berjenis true eksperimental dengan post-test only with control group design menggunakan 24 tikus wistar sebagai objek percobaan. Kemudian dibagi menjadi 4 kelompok. Kelompok K1 diberi diet standar dan aquades 2ml/200 gramBB. Kelompok K2 diberi diet standar, aquades 2ml/200 gramBB dan minyak jelantah sebanyak 3ml/200 gramBB. Kelompok K3 diberi diet standar dan air rebusan jamur kuping hitam sebanyak 2ml/200 gramBB. Dan kelompok  K4 diberi diet standar, minyak jelantah 3ml/200 gramBB, dan air rebusan jamur kuping hitam 2ml/200 gramBB. Data dianalisis dengan uji Saphiro-Wilk, Levene test, oneway ANOVA, dan Post Hoc testHasil : Diperoleh hasil yang signifikan dilihat dari rerata kelompok dengan  kadar trigliserid dimulai dari terendah yaitu kelompok K3 (81,56 mg/dL),kemudian kelompok K1 (88,88 mg/dL),kemudian kelompok K4 (98,99 mg/dL),dan terakhir kemudian kelompok K2 (129,42 mg/dL)Simpulan : Pemberian air rebusan jamur kuping hitam dapat menurunkan kadar trigliserid
PERBEDAAN NEUTROPHIL-LYMPHOCYTE RATIO PADA SUBJEK BUKAN PEROKOK, PEROKOK RINGAN DAN PEROKOK SEDANG-BERAT Nusa, Galang Bela; Widyastiti, Nyoman Suci
DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO ) Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO )

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Abstract

Latar Belakang : Merokok merupakan problem kesehatan serius yang menyebabkan angka kesakitan dan kematian yang tinggi. Paparan asap rokok yang berlangsung lama dapat memicu inflamasi pada saluran nafas dan parenkim paru perokok serta mempengaruhi jumlah dan hitung jenis leukosit. White Blood Cell Count (WBC) menggambarkan status inflamasi. Neutrofil dan limfosit merupakan bagian terbesar dari leukosit sehingga mampu menggambarkan sebagian besar respon imun tubuh. Neutrophil-Lymphocyte Ratio (NLR) dapat menggambarkan status inflamasi pada perokok.Tujuan : Membuktikan adanya perbedaan NLR antara subjek bukan perokok, perokok ringan dan perokok sedang-berat.Metode : Penelitian deskriptif analitik dengan pendekatan belah lintang dengan subjek penelitian sebanyak 36 orang yang dibagi menjadi tiga kelompok menurut klasifikasi perokok Sitepoe, yaitu kelompok subjek bukan perokok, perokok ringan dan perokok sedang berat. NLR didapat dengan membandingkan jumlah neutrofil absolut dengan jumlah limfosit absolut. Uji statistik menggunakan uji one way Anova dan uji Post Hoc Bonferroni.Hasil : Rerata NLR pada kelompok subjek bukan perokok sebesar 2,42 ± 0,51, kelompok subjek perokok ringan sebesar 3,01 ± 1,29 dan kelompok subjek perokok sedang-berat sebesar 2,02 ± 0,63. Uji Post Hoc menunjukkan ada perbedaan antara nilai NLR kelompok subjek perokok ringan dan perokok sedang berat (p=0,030) dan tidak ada perbedaan antara nilai NLR kelompok subjek bukan perokok dengan kedua kelompok lainnya (p=0,348; p=0,821).Kesimpulan : Terdapat perbedaan antara nilai NLR subjek perokok ringan dan perokok sedang-berat, namun tidak ada perbedaan antara nilai NLR antara subjek bukan perokok dengan perokok ringan maupun perokok sedang-berat.
PENGARUH PEMBERIAN JAMUR KUPING HITAM (AURICULARIA POLYTRICHA) TERHADAP KADAR KOLESTEROL TOTAL SERUM TIKUS WISTAR YANG DIINDUKSI MINYAK JELANTAH Budinastiti, Ratih; Abdurachmin, Henna Rya; Widyastiti, Nyoman Suci
DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO ) Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : DIPONEGORO MEDICAL JOURNAL ( JURNAL KEDOKTERAN DIPONEGORO )

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Abstract

Latar Belakang: Pemberian minyak jelantah berpengaruh terhadap kenaikan kadar kolesterol total (TC). Hal ini meningkatkan resiko terjadinya aterosklerosis yang berdampak pada timbulnya penyakit kardiovaskuler. Jamur kuping hitam merupakan antioksidan alami yang mengandung polisakarida, flavonoid, niacin, dan vitamin C yang dapat memperbaiki profil lipid. Tujuan: Menganalisis pengaruh pemberian air rebusan jamur kuping hitam terhadap kadar TC serum tikus wistar yang diinduksi minyak jelantah. Metode: Penelitian true experimental dengan rancangan post test only control group design ini menggunakan tikus Wistar jantan usia 12 minggu (n=24) yang secara random dibagi menjadi 4 kelompok. K1 sebagai kontrol negatif, K2 diberi minyak jelantah, K3 diberi air rebusan jamur kuping, K4 diberi minyak jelantah dan air rebusan jamur kuping. Kadar TC serum kemudian diukur dengan metode CHOD-PAP setelah perlakuan selama 28 hari. Hasil: Rerata kadar TC K1 (80,2217±3,61 mg/dL), K2 (195,8483±5,47 mg/dL), K3 (75,5800±4,02 mg/dL), dan K4 (110,8683±5,82 mg/dL); p=0,000. Simpulan: Air rebusan jamur kuping menurunkan kadar kolesterol total serum pada tikus wistar yang diinduksi minyak jelantah.
PENGARUH PEMBERIAN MINUMAN LIDAH BUAYA TERHADAP KADAR ANTIOKSIDAN TOTAL DAN PERSENTASE LEMAK TUBUH PADA SINDROM METABOLIK Silitonga, Marisi Elizabeth R.; Nugroho HS, K. Heri; Tjahjono, Kusmiyati; Widyastiti, Nyoman Suci; Afifah, Diana Nur
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 7, No 1 (2018)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.798 KB) | DOI: 10.14710/jgi.7.1.1-8

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Background: Metabolic syndrome is related to glucose metabolism disturbance (hyperglycemia), lipid (dyslipidemia), high blood pressure, and central obesity. Metabolic syndrome implicates to heart attack, diabetes mellitus, hypertension, and death. Aloe vera, well known rich of polyphenol and vitamin, has a pharmacological effect to improve insulin resistance, chronic inflammation, and oxidative stress.Objective: To prove the effect of Aloe vera-based drink toward total antioxidant concentration improvement and body fat percentage reduction in metabolic syndrome subjects.Methods: Thus study was pre-post randomized true experimental study with control group design. The subjects were divided by 2 groups, treatment group (n=19) and control group (n=19). Treatment group was given 165 g/d Aloe vera-based drink for 30 days. Both of groups were given nutrition education about metabolic syndrome management. Total antioxidant concentration and body fat percentage were assessed pre-post-test by ABTS and BIA respectively, while food intake and activity were assessed by 24-h recall and GPAQ respectively. Independent t-test and Mann Whitney test were used to compare before and after treatment between control and treatment group.Results: Total antioxidant concentration significantly improved (p=0.00) in treatment group from 1.2 to 2.0 mmol/L, while total antioxidant concentration decreased from 1.4 to 1.3 mmol/L (p=0.074) in this control group. Moreover, there was significant difference of total antioxidant between treatment and control groups in the end of study (p=0.00). Furthermore, body fat percentage was reduced significantly from 32.8 to 32,4 mmol/L (p=0.005) in treatment group, while the reduction of body fat percentage in control group was not significant from 33.1 to 33,4 mmol/L (p=0.100). There was no difference of body fat percentage between two groups after intervention (p=0.358).Conclusion: Aloe vera-based drink improved total antioxidant concentration in metabolic syndrome subjects.
EFEK SUPLEMENTASI β-CAROTENE TERHADAP KOLESTEROL TOTAL, TRIGLISERIDA DAN MALONDIALDEHID PADA TIKUS SPRAGUE DAWLEY YANG DIABET Ermawati, Devi; Rachmawati, Banundari; Widyastiti, Nyoman Suci
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 2, No 2 (2014)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.108 KB) | DOI: 10.14710/jgi.2.2.47-52

Abstract

Background : diabetes mellitus (DM) is associated with increased total cholesterol and triglyceride, also well characterized by increased malondialdehyde production. ?-carotene has antioxidant activity, glycemic and lipid control. Objective : to analyze the effect of ?-carotene on total cholesterol, triglyceride and MDA on diabetic Rattus norvegicus sprague dawley. Methods : thirty rats were randomly divided into 5 groups : 1 (STZ), 2 (STZ+?-carotene 1 mg/kg BW), 3 (STZ+?-carotene 10 mg/kg BW), 4 (STZ+?-carotene 20 mg/kg BW), 5 (normal). Streptozotocin induced intraperitoneal 40 mg/kg BW. ?-carotene was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP, total cholesterol by CHOD-PAP, triglyceride by GPO and MDA by ELISA with TBARS methods. Hypothesis test used one way anova then followed by post hoc bonferroni to analyze the efficient dose effect. Results : there was a significant difference of total cholesterol (p=0.002) after ?-carotene 10 mg/kg BW supplementation on alternate days within 30 days orally. ?-carotene 10 mg/kg BW was the most efficient dose to lowering total cholesterol. There were significant differences of triglyceride (p=0.0001) and MDA (p=0.0001) after ?-carotene 1, mg/kg BW, 10 mg/kg BW and 20 mg/kg BW supplementation on alternate days within 30 days orally. ?-carotene 10 mg/kg BW was the most efficient dose to lowering triglyceride, while 20 mg/kg BW to lowering MDA. Conclusion : ?-carotene 10 mg/kg BW is the most efficient dose to lowering total cholesterol and triglyceride. ?-carotene 20 mg/kg BW is the most efficient dose to lowering MDA.