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Comparison of Ethanol Extract from Roots, Leaves, and Flowers of Calotropis gigantea as Anticancer on T47D Breast Cancer Cell Lines Mutiah, Roihatul; Sukardiman, Sukardiman; Widyawaruyanti, Aty; Zulaikah, Siti
ALCHEMY Vol 5, No 1 (2016): ALCHEMY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.215 KB) | DOI: 10.18860/al.v5i1.3690

Abstract

This research aims to find out the anticancer activity of ethanol extract from the roots, leaves, and flowers of Calotropis gigantea. This experiment was conducted by MTT method on T47D breast cancer cell line. The result showed that the root of Calotropis gigantea was more cytotoxic (IC50 89.76 ?g/mL) on T47D breast cancer cell line than the leaves (IC50 459.51 ?g/mL) and the flowers (IC50>1000). Based on the result, roots are potent to be chemotherapeutic agent, especially in breast cancer.
Comparison of the provision of champedon trunk shell capsule extract and artesunate on placental histopathologic classification in pregnant mice (Mus musculus) malaria model Yunus, Achmad; Prasetyo, Budi; Dachlan, Erry Gumilar; Widyawaruyanti, Aty; Widjiati, Widjiati
Majalah Obstetri & Ginekologi Vol 25, No 3 (2017): December
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.244 KB) | DOI: 10.20473/mog.V25I32017.71-76

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Objectives: To compare the effect of chemphedon trunk shell capsule extract with artesunate on placental histopathologic classification in pregnant mice malaria’s models.Materials and Methods: This study was a randomized experi-mental laboratory study in BALBc strains mice with controls. A total of 30 pregnant mice were divided into 3 groups. On day 10 each group was infected with P. berghei. Furthermore, on day 11 each group was subjected to thin blood smear examination and subsequent infection when administered antimalarial positive. Group P1 received Chemphedon trunk shell extract 100 mg/kg BW/day per sonde two times per day for 5 days. Group P2 received 36.4 mg artesunate/kg BW/day for 3 days followed by CMC Na per sonde for 2 days, and group P3 received placebo (CMC Na) for 5 days. On day 16 the mice were dissected on 16 days of pregnancy and the placenta was taken and preparations were made to observe histopathological classification of the placenta according to Rogerson. Grouping was performed accor-ding to placental histopathological classification by Rogerson.Results: Groups receiving cemphedon trunk shell capsule extract, artesunate and placebo revealed p=0.004 (p<0.05), showing that there were two groups with significant difference. To determine which group had significant difference, the test was followed by Mann-Whitney post-hoc test. The results showed chempedon trunk shell capsule group and placebo obtained p=0.007 (p<0.05), indicating significance. Artesunate and placebo groups revealed p=0.003 (p<0.05), also indicating significance. The test results of chemphedon trunk shell capsule extract and artesunate groups showed p=0.475 (p<0.05), indicating no significant differences.Conclusion: Placental histopathologic classification on pregnant mice malaria’s model that received antimalarial chemphedon trunk shell capsule extract of 100 mg/kg BW is better than place-bo and equivalent to artesunate of 36.4mg/kg BW.
Sambiloto (AS201-01) is better than standard antimalarial drug (DHP) in reducing Toll-Like Receptor 2 (TLR2) on placenta malaria model Hamidah, Masyitah; Widyawaruyanti, Aty; Widjiati, Widjiati; Prasetyo, Budi
Majalah Obstetri & Ginekologi Vol 26, No 2 (2018): August
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.733 KB) | DOI: 10.20473/mog.V26I22018.74-82

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Objectives: To compare the TLR2 expression in the placenta between treated by sambiloto EA-96 fraction tablet (AS201-01) and dihydroartemisinin-piperaquine phosphate (DHP)Materials and Methods: Experimental study using 24 pregnant mice. All sample divided into 4 groups with randomization are uninfected group, Plasmodium infected group and given placebo, sambiloto (AS201-01) and DHP. Then performed surgery and placental sampling were staining with adopting in tunnel assay method to measure the TLR2 expression of placental.Results: The expression of TLR2 in uninfected group has the lowest rate compared to other groups. The infected and placebo treated group has the highest TLR2 expression campared with sambiloto and DHP. The sambiloto group has not differ signi-ficantly with the group uninfected and lower than DHP.Conclusion: Tablet of sambiloto EA-96 fraction (AS201-01) decreased TLR2 expession better than with DHP tablet.
The effect of sambiloto tablet (AS201-01) on placental Chondroitin Sulfate A (CSA) expression of pregnant mice infected by Plasmodium berghei Wahdi, Nasrul; Widjiati, Widjiati; Widyawaruyanti, Aty; Prasetyo, Budi
Majalah Obstetri & Ginekologi Vol 26, No 2 (2018): August
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.829 KB) | DOI: 10.20473/mog.V26I22018.83-90

Abstract

Objectives: To determine the effect of Sambiloto tablet (AS201-01) in reducing the placental Chondroitin Sulfate A (CSA) Expression of pregnant mice infected Plasmodiumberghei.Materials and Methods: Experimental study using 24 pregnant mice were divided into 4 groups with randomization. Uninfected group, the placebo group, the Sambiloto tablet (AS201-01) group and the DHP tablet (as a standart drug) group. The last three groups, were infected with P. bergheion day 9th of pregnancy, and the treatment was started at day 11th of pregnancy, and samples were terminated at day 15th of pregnancy by surgery. Placental sampling were stained with Tunnel assay to measure placental CSA antibodies.Results: The placental Chondroitin Sulfate A (CSA) expression. Uninfected group compared to Sambiloto tablet (AS201-01) groups was not significantly different (p>0.05), uninfected group compared with the other treatment groups differ meaningfully (p<0.05). Placebo group compared with all groups significantly different (p<0.05). Sambiloto tablet (AS201-01) group compared to uninfected group (p>0.05) was not significantly different, with another group was significantly different (p<0.05). DHP tablet group compared to all the groups was significantly different (p<0.05).Conclusion: Placental Chondroitin Sulfate A (CSA)expression of mice infected by Plasmodium berghei treated with Sambiloto tablet (AS201-01) lower than DHP tablet. 
MEKANISME AKSI α-MANGOSTIN DARI GARCINIA TETRANDA PIERRE TERHADAP P.FALCIPARUM SECARA IN VITRO Kuncoro, Hadi; Widyawaruyanti, Aty; Ersam, Taslim
Journal of Tropical Pharmacy and Chemistry Vol 1 No 1 (2010): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.193 KB) | DOI: 10.25026/jtpc.v1i1.6

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ABSTRACT &nbsp; Development stage of parasite resistance assay and the Globin accumulation using SDS-PAGE Method has been done to parasite P. falciparum by giving α-mangostin from G. tetranda. α-Mangostin cause delays in the development stage of the parasite P. falciparum from stage schizont to stage trofozoit and cause parasite death after 48 hour. Swelling of the parasite of abnormal parasite food vacuole occurred after administration of α-Mangostin show from the results of SDS-PAGE. &nbsp; Key word : α- Mangostin, G. tetranda, P. falciparum,&nbsp; globin accumulation &nbsp; ABSTRAK &nbsp; Telah dilakukan pengujian hambatan perkembangan stadium parasit dan akumulasi globin menggunakan metode SDS- PAGE terhadap P. falciparum dengan pemberian α-Mangostin dari G. tetranda. Diketahui α-Mangostin menyebabkan penundaan perkembangan parasit P. falciparum dari stadium trofozoit menjadi stadium skizon dan menyebabkan parasit mati setelah jam ke 48. Pembengkakan vakuola makanan parasit yang abnormal terjadi setelah pemberian α- Mangostin terlihat dari hasil SDS-PAGE. &nbsp; Kata Kunci : α-Mangostin, G. tetranda, P. falciparum, , akumulasi globin
Skrining Aktivitas Antimalaria Beberapa Tanaman Indonesia Hasil Eksplorasi Dari Hutan Raya Cangar, Batu-Malang, Jawa Timur Hafid, Achmad Fuad; Puliansari, Nike; Lestari, Nur Suci; Tumewu, Lidya; Rahman, Abdul; Widyawaruyanti, Aty
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol 3, No 1 (2016): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Fakultas Farmasi Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.387 KB) | DOI: 10.20473/jfiki.v3i12016.7-11

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Background: Malaria is the most important parasitic disease. Malaria control which depends on specific chemotherapy now complicated by resistance of Plasmodium falciparum to most commonly available antimalarial drug. Such situation has heralded the need for alternative antimalarial therapy. Objective: This research aim was to find new antimalarial candidates from some Indonesia plants collected from Cangar National Forest, Batu-Malang, East Java. Methods: Eleven samples of leaves and stem extracts were screened against Plasmodium falciparum 3D7 culture which maintained in RPMI-1640 Medium. Samples tested in concentration of 0.01, 0.1, 1, 10 and 100 µg/ml. Probit analysis was used to determine IC50. Results: In vitro antimalarial activity revealed that only three crude extracts samples from Fraxinus griffithi stem extract, Piper sulcatum leaves extract and Eucalyptus globulus stem extract had good antimalarial activity with IC50 value of 0.33, 0.20 and 0.55 µg/ml, respectively. Conclusions: Fraxinus griffithi stem extract, Piper sulcatum leaves extract and Eucalyptus globulus stem extract might be a good candidate for antimalarial natural product resources.
Comparison of the effect of sambiloto (AS 201-01) tablet and dihydroartemisinin-piperaquine on macrophage MIF expression in mice placenta infected with Plasmodium berghei Aryani, Desak Ketut Ayu; Prasetyo, Budi; Widyawaruyanti, Aty; Widjiati, Widjiati
Majalah Obstetri & Ginekologi Vol 26, No 3 (2018): December
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.201 KB) | DOI: 10.20473/mog.V26I32018.100-106

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Objective: To compare the administration of sambiloto tablets (AS201-01) and dihydroartemisinin-piperaquine phosphate tablets in pregnant mice infected by P. berghei on the expression of MIF in the placenta.Materials and Methods: Experimental laboratory research, 24 pregnant mice were divided into 4 groups with randomization, ie. non-infected, placebo, sambiloto (AS201-01) and DHP groups. On day 9 P. berghei was infected, on day 11 the treatment was given, day 15 the surgery was performed, placental samples were taken, immunohistochemical staining was given, and MIF expression was assessed.Results: The expression of MIF in the group not infected with P. berghei had the lowest mean, while the highest mean was found in the placebo group. Uninfected groups were not significantly different from sambiloto (AS201-01) tablet group. Sambiloto tablet group (AS201-01) had lower MIF expression than DHP group, but it was not significantly different. Sambiloto tablet group (AS201-01) showed lower MIF expression than placebo. MIF expression in DHP group was lower than that in placebo group. From the lowest, the MIF expressions were as follows: group not infected with P. berghei, group receiving sambiloto (AS201-01) tablet, DHP group and placebo group.Conclusion: MIF expression in the placenta of pregnant mice infected with P. berghei and receiving sambiloto (AS20-01) tablets was not different from those receiving DHP tablets.
Aktivitas Antimalaria Ekstrak Kulit Batang Cempaka Kuning Terhadap Plasmodium Falciparum 3D7 ARIANTARI, NI PUTU; RUSTINI, NI LUH; TUMEWU, LIDYA; HAFID, ACHMAD FUAD; WIDYAWARUYANTI, ATY
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1005.646 KB)

Abstract

Cempaka kuning (Michelia champaca) merupakan tanaman dari suku Magnoliaceae, yang secara tradisional telah digunakan untuk pengobatan demam. Penelitian ini dilakukan untuk mengevaluasi aktivitas antimalaria ekstrak kulit batang cempaka kuning secara in vitro terhadap Plasmodium alcipamm 3D7. Kulit batang cempaka kuning diekstraksi dengan n-heksana, kloroform dan metanol sehingga diperoleh ekstrak n-heksana, ekstrak kloroform dan ekstrak metanol. Ketiga ekstrak tersebut selanjutnya diuji aktivitas antimalarianya. Skrining titokirnia terhadap ketiga ekstrak tersebut menunjukkan bahwa ekstrak n-heksana mengandung minyak atsiri dan terpenoid, ekstrak kloroform mengandung minyak atsiri, terpenoid dan llavonoid, serta ekstrak metanol mengandung minyak atsiri, terpenoid, flavonoid, tanin dan glikosida. Hasil penelitian ini juga menunjukkan bahwa ketiga ekstrak yang diuji aktif secara in vitro terhadap P. falciparum 3D7, dengan nilai IC50 masing-masing sebesar 0,36 µg/mL untuk ekstrak n-heksana; 0,24 µg/mL untuk ekstrak kloroform dan 1,00 µg/ mL untuk ekstrak metanol. Ekstrak kulit batang Cempaka kuning memiliki aktivitas antimalaria sangat baik dan potensial untuk dikembangkan sebagai antimalaria.
Anti-Breast Cancer Potency of Multistage Extraction from Jamur Dewa (Agaricus blazei Murill) Solvents on MCF-7 Cells Misgiati, Misgiati; Sukardiman, Sukardiman; Widyawaruyanti, Aty
Indonesian Journal of Cancer Chemoprevention Vol 8, No 2 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss2pp68-73

Abstract

ABM (Agaricus blazei Murill) is a basidiomycetes fungus. ABM is used by people for the treatment of diabetes, antihypertention, anticholesterol, anticancer, and immunostimulant. ABM contains terpene, steroids, agaritine, vitamin C, vitamin E, and betaglucane. In this research, ABM extract was tested as an anti-breast cancer in vitro using MCF-7 breast cancer cells. The extract was obtained from the multistage extraction process of several solvents in turn, the solvent used, among others, n-hexane, dichloromethane (DCM), chloroform, ethyl acetate, butanol, and water. The results of the research were the obtained IC50 value from n-hexane extract 247,17 μg /ml; extract DCM 227μg/ml ; chloroform extract 215,64 μg /ml ; extract of ethyl acetate 234,9 μg/ml ; butanol extract 500,78 μg/ml; while the water extract was inactive. Based on these results can be considered for further research to fractionate in order to know which class compounds have the potency as anticancer within the extracts.Key words : Agaricus blazei, multistage extraction, MCF-7 cells.
Profil Fitokimia Ekstrak Etanol Bunga Kasumba Turate (Carthamus tinctorius L.) yang Berpotensi Sebagai Antimalaria Hamsidi, Rini; Widyawaruyanti, Aty; Hafid, Achmad Fuad; Ekasari, Wiwied; Malaka, Muhammad Hajrul; Kasmawati, Henny; Akib, Nur Illiyyin; Wahyuni, W; Sabarudin, S
Pharmauho Vol 4, No 2 (2018): Pharmauho
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.085 KB)

Abstract

Bunga Kasumba turate (Carthamus tinctorius Linn) dari suku Asteraceae merupakan tumbuhan obat tradisional etnis Sulawesi Selatan yang secara empiris digunakan dalam pengobatan campak. Ekstrak etanol dari kasumba turate memberikan peningkatan aktivitas imunoglobulin G (IgG) dan  aktivitas imunoglobulin A (IgA) yang sangat signifikan. Penelitian menunjukkan ekstrak etanol bunga C. Tinctorius L memiliki potensi sebagai antimalaria secara in vitro dengan nilai IC50 sebesar 1,06 µg/ml. Sampel bunga C. tinctorius L. kering sebanyak 3 Kg dicacah hingga menjadi serbuk sebanyak 870 gram kemudian diekstraksi secara maserasi dengan menggunakan pelarut etanol 80%. Hasil maserasi dipekatkan dengan vacuum rotary evaporator pada suhu 50°C sehingga diperoleh ekstrak kental sebanyak 178,7gram. Profil fitokimia menunjukkan ekstrak etanol bunga C. tinctorius L. mengandung senyawa metabolit antara lain saponin, terpenoid, flavonoid, tanin dan antrakuinon.Kata kunci: Skrining, ekstrak, kasumba turate, obat tradisional, antimalaria