Ni Made Armini Wiendi
Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

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Perbanyakan In Vitro Dendrobium Indonesia Raya ‘Ina’ melalui Embriogenesis Somatik Berbasis Sistem Bioreaktor Rachmawati, Fitri; Wiendi, Ni Made Armini; Mattjik, Nurhajati Ansori; Purwito, Agus; Winarto, dan Budi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.183 KB) | DOI: 10.24831/jai.v44i3.12816

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ABSTRACTAn effective and efficient in vitro propagation system has important roles in preparing and producing high quality-seedlings of Dendrobium for commercial scale. The objective of this research was to establish an effective and efficient embryogenic callus (EC) proliferation method using bioreactor system and regeneration EC into plantlet for producing high quality seedlings of Dendrobium Indonesia Raya ‘Ina’. Differences in callus densities (5, 10, 15, and 20 g callus in 250 mL medium), aeration levels (2.5, 5.0, and 10.0 O2 volume  per  medium volume per minute; vvm), and regeneration media half-strength MS and 2 g L-1 NPK (32:10:10) combinated by 0.00, 0.05 mg L-1 BA, 150  mL L-1 coconut water and their combinations were tested in this experiment. The experiments were arranged using randomized completely block design (RCBD) with three replications for EC proliferation and randomized completely desaign (RBD) for EC regeneration. The results showed that combination of  aeration at 2.5 vvm and 10 g of EC was the most suitable aeration level and callus density for proliferation of EC in the 500 ml airlift bioreactor with 6.85 multiplication rate, 92.5% EC formation, and malformed callus morphology as low as 6.1%. The highest somatic embryos (SEs) formation was 87.7% with 44.5 SEs per clump and 92.1% SEs germination with 41.0 germinated-SEs per clump, 85.1% normal germinated-SEs, and whereas the best performance of plantlet was obtained from 1/2 MS + 0.05 mg L-1 BA semi solid medium. Plantlets were successfully acclimatized using Cycas rumphii medium with high survival rate (91.6%). Keywords: aerations, callus densities, germination, media, somatic embryos
Keragaman Genetik Kacang Bogor (Vigna subterranea L. Verdc.) Berdasarkan Marka SSR (Simple Sequence Repeat) Illahi, Zikril; Wiendi, Ni Made Armini; Sudarsono, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.993 KB) | DOI: 10.24831/jai.v44i3.12787

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ABSTRACTBambara groundnut (Vigna subterranea L. Verdc.) is an important underutilized legume crop in Indonesia. The aim of this research is to study genetic diversity of bambara groundnut from Sukabumi and Sumedang, West Java, Indonesia. This study used 107 bambara groundnut accessions, which consisted of 57 accessions from Sukabumi and 50 accessions from Sumedang. We use five simple sequence repeat (SSR) to analyze the accessions. Totally nine alleles were detected, with a mean of 1.8 alleles per locus. Allelic and gene diversities were higher in Sumedang (1.8 alleles per locus and 0.119) than in Sukabumi population (1.4 alleles per locus and 0.020), respectively. We constructed a phylogenic tree by Neighbor-Joining analysis based on genetic distances (DA) and showed the tree divided bambara groundnut accessions into two broad groups according to the origin of samples (Sukabumi and Sumedang). Results from the phylogenic tree are in line with those from the population structure analysis. Keywords: allele diversity, bambara groundnut, genetic distance, under utilized legume
Proliferasi In Vitro Plb Anggrek Dendrobium lasianthera Hasil Induksi Mutasi Genetik dengan Kolkisin Melalui Penambahan Benzyl Adenine Bawonoadi, Gilar; Wiendi, Ni Made Armini; Krisantini, .
Buletin Agrohorti Vol. 5 No. 2 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (705.327 KB) | DOI: 10.29244/agrob.v5i2.16791

Abstract

Dendrobium lasianthera merupakan spesies anggrek yang endemik di Papua. Proliferasi atau perbanyakan tanaman melalui kultur jaringan merupakan salah satu alternatif dalam memperbanyak spesies tanaman dengan jumlah individu yang terbatas. Keragaman karakteristik pada tanaman hias, baik yang bersifat alami atau hasil dari induksi mutasi, dianggap penting dalam menentukan nilai ekonomisnya. Pengembangan protokol produksi membutuhkan penelitian terkait media yang sesuai untuk memperbanyak tanaman. Penelitian dilakukan dengan mengkulturkan eksplan anggrek Dendrobium lasianthera berupa plb (protocorm-like body) dari anggrek D. lasianthera yang telah diinduksi mutasi melalui perendaman dalam larutan mutagen kolkisin pada penelitian sebelumnya. Pengamatan dilakukan terhadap kemampuan pertumbuhan 960 eksplan dari 16 kombinasi perlakuan (termasuk kontrol) perendaman kolkisin ditumbuhkan pada media MS0 yang ditambahkan sitokinin BA (6-Benzyladenine) sebanyak 1 mgL-1 dan 2 mgL-1. Hasil penelitian menunjukkan bahwa eksplan dengan kombinasi perlakuan perendaman yang berbeda memiliki kemampuan pertumbuhan yang berbeda secara nyata, dilihat dari jumlah daun, akar, plb dan tunas baru yang terbentuk selama periode pengamatan.Perbedaan dalam konsentrasi BA dalam media tidak menunjukkan respon yang berbeda nyata dalam jumlah daun dan akar baru yang terbentuk, namun nyata meningkatkan jumlah tunas baru yang terbentuk dan mempercepat pembentukan tunas. Eksplan yang ditumbuhkan pada media BA 1 mgL-1 memiliki rata-rata waktu awal pembentukan tunas yang lebih singkat dibandingkan dengan media BA 2 mgL-1. Beberapa planlet hasil induksi mutasi menunjukkan perbedaan fenotipe dari planlet kontrol berupa bentuk daun yang berbeda.
Pembentukan Embrio Endospermik Sekunder Mangga (Mangifera indica L.) Gedong Gincu Klon 289 Hindaningrum, Irni Furnawanthi; Wiendi, Ni Made Armini; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.889 KB) | DOI: 10.24831/jai.v42i2.8434

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ABSTRACTThe improvement of Mangifera indica L. by conventional breeding approaches has been confounded by the long generation cycle, low fruit set, single seed per fruit and high degree of cross pollination. Biotechnology complements conventional breeding and expedite the mango improvement programs. Endosperm culture is a direct method to produce triploid plants. This study aimed  to obtain embryo from endosperm culture. The system of secondary somatic embriogenesis in mango described here represents a source of embryogenic material may be used for mass propagation and genetic manipulation of this crop. The method consisted of induction, proliferation, maturation, germination, and histological analysis of the obtaimed embryos. A protocol for plantlet regeneration was developed for Gedong Gincu mango clone 289 through secondary somatic embryogenesis. Primary somatic embryos (proembryo and cotyledonary embryos) were cultured in induction medium to induce the secondary somatic embryos. The best proliferation rate was 0.22 in medium with 1 g L-1 Poly Vinyl Pyrrolidone (PVP) for multiplication of secondary somatic embryos. Maturation of inoculum derived from the proliferation medium supplemented with 2 g L-1 of activated charcoal on medium containing 0.4 mg L-1 BAP provides the average 2.39 embryo formation of cotyledonari phase. The highest germination frequency (20%) was obtained in media with GA3 1.5 mg L-1.Keywords: endosperm, Gedong Gincu, Mangifera indica L, secondary endospermic embrio
Proliferasi Tunas Adventif Tagetes (Tagetes erecta L.) Kultivar African Crackerjack dengan BAP, GA3, dan IAA Secara In Vitro Baidowi, Muhammad; Wiendi, Ni Made Armini
Buletin Agrohorti Vol. 5 No. 1 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.428 KB) | DOI: 10.29244/agrob.v5i1.15893

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Tujuan penelitian ini mempelajari proliferasi tunas adventif eksplan hipokotil, epikotil, dan kotiledon Marigold (Tagetes erecta L.) secara in vitro dengan kombinasi BAP, GA3, dan IAA. Percobaan pertama adalah mengenai sterilisasi benih. Eksplan percobaan dua dan tiga adalah epikotil, hipokotil, dan kotiledon dari kecambah aseptik. Eksplan epikotil berhasil terproliferasi sedangkan hipokotil dan kotiledon tidak terproliferasi karena oksidasi fenol. Interaksi BAP dan IAA berpengaruh nyata pada induksi kalus epikotil pada semua minggu pengamatan, sedangkan interaksi BAP dan GA3 tidak berpengaruh nyata pada semua parameter. Komposisi media MS ditambah IAA dan BAP berhasil menginduksi kalus 70.7% dari eksplan epikotoil, 49.3% dari eksplan kotiledon, 53% dari hipokotil. Komposisi media MS ditambah GA3 dan BAP berhasil menginduksi kalus 60% dari eksplan epikotoil, 48% dari eksplan kotiledon, 46% dari hipokotil. Pada percobaan dua, media MS dengan 0.87 mg L-1 IAA + 0.5 mg L-1 BAP dan media MS dengan 0.43 mg L-1IAA + 0.5 mg L-1BAP terbaik dalam menginduksi tunas adventif eksplan epikotil. Pada percobaan tiga, proliferasi tunas adventif tertinggi pada media MS dengan 1.73 mg L-1GA3 + 1.5 mg L-1 BAP. IAA berpengaruh nyata pada eksplan bertunas pada satu 1 MSP, tunas per eksplan pada 3 MSP, dan daun pertunas pada 4 MSP dan 5 MSP, sedangkan BAP berpengaruh nyata pada induksi kalus pada 1 MSP, eksplan bertunas pada 3 MSP, dan tunas per eksplan pada 3 MSP. Pada percobaan tiga, GA3 berpengaruh nyata pada pembentukan akar pada 4 MSP dan 5 MSP, sedangkan BAP berpengaruh nyata pada proliferasi tunas per eksplan pada 3 MSP.
Deteksi Kestabilan Genetik Ramet Kelapa Sawit Hasil Kultur In Vitro Menggunakan SSR Cahyaningsih, Yuni Fitri; Wiendi, Ni Made Armini; Toruan-Mathius, dan Nurita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 1 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.736 KB) | DOI: 10.24831/jai.v44i1.12507

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ABSTRACTCommercial production of oil palm ramet requires the guarantee of high genetic stability. The objectives of this research were to determine 1) genetic diversity of ortet as source of explant, and 2) genetic stability of ramet derived from ortet propagated through tissue culture. Genetic stability analysis was done using ramet from five Tenera (D×P) oil palm ortets.As many as 20 ramets were randomly chosen from each ortet. A total of 100 ramets were used for genetic stability analysis. Genetic similarity analysis was analyzed using NTSyspc version 2.1 software with method Similarity for Qualitative Data and Unweighted Pair Group Method Aritmatic (UPGMA). The results indicated 20 SSR primer pairs were polymorphic and could form 44 alleles. As many as 80% of ramets from IS 3 ortet showed genetic similarity ranged from 97-100% to the ortet. All ramets derived from IS 10, IS 20 and IS 40 ortet had 90-100% of genetic similarity to its respective ortet. Futhermore, 95% of ramets from IS 39 ortet had 97-100% of genetic similarity to the ortet.Keywords: Elaeis guineensis Jacq., genetic similarity, tissue culture
Pengaruh Pemberian Auksin (NAA) dengan Sitokinin (BAP, Kinetin dan 2ip) terhadap Daya Proliferasi Tanaman Kantong Semar (Nepenthes mirabilis) Secara In Vitro Yudhanto, Bagus Setyo; Wiendi, Ni Made Armini
Buletin Agrohorti Vol. 3 No. 3 (2015): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.589 KB) | DOI: 10.29244/agrob.v3i3.15799

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Auksin dan sitokinin umumnya digunakan sebagai zat pengatur tumbuh secara in vitro. Sudah ditemukan bahwa kombinasi auksin (NAA) dan sitokinin (BAP, kinetin, dan 2ip) untuk proliferasi tunas Nepenthes mirabilis secara in vitro. Penelitian ini dirancang menggunakan rancangan acak lengkap dua faktor, auksin sebagai faktor pertama dan sitokinin sebagai faktor kedua. Kombinasi auksin dan sitokinin yang digunakan adalah NAA: 1 dan 2 mg / l sedangkan BAP, kinetin, dan 2ip masing-masing sebanyak 0, 2,5, dan 5 mg / l. Kombinasi zat pengatur tumbuh yang ditambahkan ke media Murashige dan Skoog (MS). Hasil terbaik untuk proliferasi tunas yaitu perlakuan tanpa sitokinin dan tambahan NAA 1 mg / l sebanyak 5,2 tunas dalam 10 minggu.
PERBANYAKAN IN VITRO TANAMAN BAWANG PUTIH (Allium sativum L.) VARIETAS LUMBU PUTIH MELALUI INDUKSI TUNAS ADVENTIF Wiendi, Ni Made Armini; Wattimena, G. A.; Prasetyanti, Enny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 24 No. 1 (1996): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1252.754 KB) | DOI: 10.24831/jai.v24i1.1617

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Two sets of experiments were conducted to determine the effect of growth hormones, argynine, and coconut water, on the adventitious shoot induction from garlic tissue, and also to find out the best medium for adventitious shoots proliferation. Both experiments could induce direct adventitious shoot and indirect adventitious shoot formation, from calli. Medium with 2 ppm Kinetin and 0.4ppm 2,4-Dproduce good quantity and quality of shoots. The number of shoot from this medium were 32.6 shoots per explant. Medium with 0.5 ppm Kinetin, 0.1 ppm 2.4-D, 25 ppm Argynine, and 10% coconut water produce the highest diameter and good quality of calli, while medium with 1 ppm 2iP and 25 ppm Argynine induced adventitious shoot from calli and produced the highest number of shoot per culture (33.9 shoots).
Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri Nurhasanah, ,; Wattimena, G. A.; Purwito, Agus; Wiendi, Ni Made Armini; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 2 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (888.396 KB) | DOI: 10.24831/jai.v31i2.1467

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Hordothionins are small anti-bacteria proteins present in barley endosperm, To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Agrobacterium tumefaciens strain LBA 4404, under the control of a cauliflower mosaic virus (CaMV) 358 promotor. The in vitro grown stem (internodus) was used in this research. After 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesific primer from CaMV 358 promotor showed the presence of amplified T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested, The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level, There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible. Key words: Potato, Hordothionin gene, Disease resistance
Photoautotrophic System: A Review and Potential Application for Plant Propagation In Vitro Krisantini, Krisantini; Wiendi, Ni Made Armini
Journal of Tropical Crop Science Vol 5 No 2 (2018): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.007 KB)

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AbstractThe standard method of in vitro plant micro propagation uses of tightly closed culture bottles using agar media containing macro and micro nutrients and sucrose as a source of carbon for the explants. The closed bottle culture is usually kept in a temperature and light controlled environment which is lower and of different quality from the natural sunlight, resulting in high relative humidity and no air exchange inside the bottles.  Explants produced in vitro have malfunctioned stomata, undeveloped cuticles and lower leaf chlorophyll levels, and hyper hydration of the plantlets. Photoautotrophic tissue culture is micro propagation without or with a reduced sugar level in the culture media, so the growth or accumulation of carbohydrates of the explants is dependent fully upon photosynthesis and inorganic nutrient uptake. This method is usually combined with ventilation or CO2 enrichment, and recently, with incorporating porous materials such as vermiculite, gum or paper pulp to the agar media to promote better root system of the explants. This article discuss the advantages and disadvantages of the photoautotrophic micro propagation compared to the standard micro propagation methods, and provided the results of the photo autotrophic micro propagation studies conducted at Laboratory of Tissue Culture II of the Department of Agronomy and Horticulture, Bogor Agricultural University, Indonesia.