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The Efficacy and Safety of Topical Gel Formulation of n-Hexane Fraction of Curcuma longa in Wound Healing of Hyperglycemic Mice Wientarsih, Ietje; Winarsih, Wiwin; Sutardi, Lina Noviyanti
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Curcuma longa (turmeric) is one of the tropical plant belong to Zingiberaceae family. It has beneficialeffects in healing of variety diseases. The aim of this study was to evaluate the efficacy and safety of  atopical gel formulation of n-hexane fraction of Curcuma longa in the wound healing of hyperglycemic mice.A total of 30 hyperglycemic mice were divided into three groups. They were given topical application, NC asa negative control (gel base), PC as a positive control (Neomycine sulfate 5%), treated groups (HF = n-hexane gel). Histopathological examination of the skin was performed to evaluate the wound healing.  Theresult showed that, there were no changes on tha appearance of the gel preparation, physically.  Evethoughupon the pH determination of the gel at six month of storage at 40ºC there were significant difference.  Theeffect of n-hexane gel, in terms of decreasing the surface area of wound and histopathological characteristicswere significantly (p <0.05) different between the PC group and HF group.  Skin irritant test showedneither rashes, swelling, inflammation, redness, irritation, itching nor any other toxicity  symptoms followingapplication of the gel. Significant effect of n hexane fraction of C. longa gel on wounds without any adversedermal toxic effects sounds very promising and it may be employed in the management of diabetic wound.
Uji Toksisitas Akut Ekstrak Rimpang Kunyit pada Mencit : Kajian Histopatologis Lambung, Hati dan Ginjal (ACUTE ORAL TOXICITY OF TURMERIC EXTRACT IN MICE : HISTOPATHOLOGICAL STUDIES OF STOMACH, LIVER AND KIDNEY) Winarsih, Wiwin; Wientarsih, Ietje; Sulistyawati, Nova P.; Wahyudina, Istifharany
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was carried out to determine the mouse lethal dose (MLD50 of ethanolic extract ofthe turmeric (Curcuma longa) rhizomes following single oral administration and to evaluate thetoxicopathologic effects of the extract in certain organs ie stomach, liver and kidney. Forty five male micewere divided into nine groups. Four groups were treated orally with ethyl acetate fraction, four groups weretreated orally with hexane fraction of ethanolic turmeric extract, and one group was used as control. Theethyl acetate fraction groups were administered orally with the ethyl acetate fraction at doses of 7.5, 1.5,30 and 60 g/kg body weight. The hexane fraction groups were administered orally with the hexane fractionwith the dosage of 7,5, 15, 30 and 60 g/kg body weight. The control group received normal saline. MLD50 ofethyl acetate fraction was 27,98 gram/kg body weight by per oral administration. Oral MLD50 of hexanefraction was 19,50 gram/kg body weight. Histopathological features of the ethyl acetate and hexanefractions groups showed increased amount of parietal cells in stomach and parenchymal degeneration andnecrosis in their liver and kidney.
PF-18 PHOTOMICROGRAPH OF NANOGEL ANDROGRAPHOLIDE-BETA CYCLODEXTRINE INCLUSION COMPLEX AS ANTI-BURNS Prasetyo, Bayu Febram; Wientarsih, Ietje; Sajuthi, Dondin; Juniantito, Vetnizah
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Inclusion complex is a complex formed between drug molecules which act as guest or located inside the cavity of host molecule. Host molecules are commonly originated from the derivative group of cyclodextrin. Among cyclodextrin groups, beta cyclodextrin (BCD) is mostly used in formula development and drug delivery system [1].Andrographolide (AG) is a pure isolate chemically syntesized from sambiloto herbs (Andrographis paniculata Nees), in the form of needle cystal-like which is colorless and extremely bitter. AG has variety of medical properties, particularly as anti-inflamation to treat skin burns [2]. However AG has poor solubility in water. This will result in low abillity to solute, penetrate membrane, and distribute the drug when applied transdermally in burn skin. In burn skin, there is tendency to skin damage, especially in stratum corneum which acts as semipermeable barrier. The ability of drugs that applied transdermally tends to be high.Formation of inclusion complex using AG and BCD to increase the ability of AG in penetrating membrane should be done. Transmission Electron Microscope (TEM) is a fast technique to confirm the formation of drug or inclusion complex by  comparing the shape and particle size [3]. Study on percutaneous penetration of AG-BCD inclusion complex is produced through solvent evaporation method at mole ration 1:2 in viscolam gel preparation.
Aktivitas Larvasida Biji Bengkuang sebagai Insektisida Nabati terhadap Larva Lalat Crysomya bezziana Mustika, Aulia Andi; Hadi, Upik Kesumawati; Wardhana, April Hari; Rahminiwati, Min; Wientarsih, Ietje
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.789 KB) | DOI: 10.29244/avi.4.2.68-73

Abstract

Bengkuang merupakan salah satu tanaman obat yang berpotensi sebagai bioinsektisida. Penelitian ini bertujuan mengetahui efektivitas biji bengkuang sebagai insektisida nabati terhadap larva lalat Crysomya bezziana (C. bezziana) agen penyebab miasis secara in vitro. Penelitian ini terbagi menjadi 5 kelompok perlakuan. Masing-masing sebanyak 20 Larva instar 1 (L1), Larva instar 2 (L2), dan Larva instar 3 (L3) C.bezziana digunakan untuk pengujian in vitro menggunakan pot plastik yang berisi media larva dan ekstrak ethanol biji bengkuang dengan konsentrasi bertingkat 0,06, 0,12, dan 0,25%. Coumaphos 0,06% dan akuades steril digunakan sebagai kontrol positif dan negatif. Hasil penelitian menunjukkan bahwa pada konsentrasi 0,25% mampu menyebabkan 100% kematian larva dan 100% pupa tidak menetas. Pengujian L3 menunjukkan bahwa ekstrak ethanol biji bengkuang mampu menyebabkan penurunan daya tetas pada semua konsentrasi. Pengujiaan L1 dan L2 untuk mengindikasikan efektifi tas ekstrak sebagai racun perut, sedangkan pengujian pada L3 sebagai indikasi racun kontak. Biji bengkuang memiliki daya larvasida terhadap beberapa jenis larva serangga C. bezziana.
Biomass and Carbon Mass Allometric Equation Models of Avicennia marina (Forsk.) Vierh. Case Study Pulau Dua Natural Reserve Banten PERSAMAAN ALOMETRIK BIOMASSA DAN MASSA KARBON Avicennia marina (Forsk.) Vierh. Studi Kasus Cagar Alam Pulau Dua Banten Lestari, Tyas Ayu; Rahadian, Aswin; Purwanto, M. Yanuar J.; Wientarsih, Ietje
Jurnal Silvikultur Tropika Vol. 7 No. 2 (2016): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan, Institut Pertanian Bogor (IPB)

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Abstract

Research measurements of carbon stocks Avicennia marina has been done by Dharmawan and Siregar (2008) with a DBH 5 cm. Calculation of carbon stocks Avicennia marina with DBH less than 5 cm hadn’t been done. Currently, approaches to measuring biomass and carbon mass mangrove species is being developed through allometric equation. Allometric equation to estimate carbon stocks are still limited, especially for mangrove with DBH less than 5 cm. Therefore, this research carried out calculations to obtain allometric equations for estimating biomass and carbon mass Avicennia marina with DBH less than 5 cm. The results of research can complement allometric equations that have been published previously. Allometric equations constructed using two equations, namely linear and logarithmic with two variables such as total height (x1) and diameter at breast height / DBH (x2). Allometric equation to estimated total biomass, root, stem, branch and leaves are Log YTotal = -7.92 + 2.02 Log (Tt), Log YRoot = -8.37 + 1.94 Log (Tt), Log YStem = -8.83 + 1.99 Log (Tt) + 0.419 Log (DBH), Log YBranch = -8.63 + 2.01 Log (Tt), dan Log YLeaves = -7.73 + 1.63 Log (Tt). Allometric equation to estimated total carbon root, stem, branch and leaves are Log YTotal = -8.82 + 2.21 Log (Tt), Log YRoot = -9.11 + 2.04 Log (Tt), Log YStem = -8.89 +2.06 Log (Tt) + 0.467 Log (DBH), Log YBranch = -9.41 + 2.13 Log (Tt), dan Log YLeaves = -8.46 + 1.64 Log (Tt).Key words: Seedling, stake, total height, diameter at breast height
Kombinasi Imbuhan Herbal Kunyit dan Zink dalam Pakan sebagai Alternatif Pengobatan Kolibasilosis pada Ayam Pedaging (THE COMBINATION OF CURCUMIN WITH ZINC IN FEED AS ALTERNATIF THERAPY COLLIBACIILOSIS IN BROILER ) Wientarsih, Ietje; Widhyari, Sus Derthi; Aryanti, Tika
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of antibiotics is one way to reduce the number of deaths in poultry industry. However, use maybe reduced because the impact for consumers. This study aims to find out the effect of the combinationbetween herbal turmeric (Curcuma domestica Val.), Garlic (Allium sativum Linn.) with Zn in the feed tothe number of erythrocyte, hematocrit and hemoglobin in chickens that is infected with Escherichia colibactery. Two hundred head of DOC were divided into 5 treatments and 4 repetitions, each repetitionconsisted of 10 chickens. Ration treatment K0 (basal ration as a healthy chickens), K (negative control)(basal ration chickens infected with Escherichia coli), P1 (basal ration + 1.5% turmeric powder + 180 ppmof ZnO / chickens infected with Escherichia coli), P2 (basal ration + 2.5% garlic powder + 180 ppm of ZnO /chickens infected with Escherichia coli), and K (positive control) (a basal ration + antibiotic / chickensinfected with Escherichia coli). At the age of 3 weeks, all treatment groups were challenged with E. coliwhich treated  orally at a dose of 108 CFU/mL. K(+) group was given antibiotics a day after the challenge. Antibiotics are given for 3 days with a dose of medication. Blood sampling performed at the age of 3 weeks(before the challenge), 1 and 2 weeks after the challenge. The blood test includes the number of erythrocytes,hematocrit values and level of hemoglobin  . The results showed the number of erythrocytes, hematocritvalues, and level of hemoglobin at treatment P1 (ration basal + 1.5% turmeric powder + 180 ppm of ZnO+ infected with Escherichia coli) tend to be higher than other treatments and have equal outcomes withtreatment K(+) (ration basal + antibiotics + infected with Escherichia coli). From the results of this studyconcluded that the use of combinations of herbs turmeric and Zn in the feed can be used as an alternativeof colibacillosis treatment.
Gambaran kristal urin pada hewan model nefrolitiasis hasil induksi etilen glikol Purwono, Rini Madyastuti; Wientarsih, Ietje; Widodo, Setyo; Purwaningsih, Erni H; Harlina, Eva
ARSHI Veterinary Letters Vol 3, No 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.24 KB) | DOI: 10.29244/avl.3.1.19-20

Abstract

Tingginya tingkat keterulangan kasus urolitiasis baik pada hewan maupun manusia, menuntut penelitian dalam mencari solusi menurukan angka keterulangan. Penelitian urolitiasis membutuhkan hewan model urolitiasis yang sesuai dengan kondisi sebenarnya. Salah satu metode yang digunakan adalah menciptakan kondisi hiperoksaluria. Penggunaan induksi etilen glikol menjadi metode yang paling umum digunakan. Induksi ini menghasilkan kristal kalsium oksalat monohidrat. 
AKTIVITAS SEDIAAN SALEP EKSTRAK BATANG POHON PISANG AMBON (Musa paradisiaca var sapientum) DALAM PROSES PERSEMBUHAN LUKA PADA MENCIT (Mus musculus albinus) Febram, Bayu; Wientarsih, Ietje; Pontjo, Bambang
Majalah Obat Tradisional Vol 15, No 3 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.515 KB) | DOI: 10.14499/mot-TradMedJ15iss3pp121 – 137

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui aktivitas pemberian ekstrak dari batang pohon pisang Ambon (Musa paradisiaca var sapientum) dalam bentuk sediaan salep terhadap proses persembuhan luka pada kulit mencit (mus musculus albinus) melalui pengamatan patologi anatomi dan histopatologi.  Hewan yang digunakan dalam penelitian ini adalah mencit dengan strain DDY umur 4-6 minggu sebanyak 45 ekor yang dibagi menjadi tiga kelompok, yaitu kelompok kontrol negatif (salep placebo), kontrol positif (salep komersil) dan salep ekstrak batang pohon pisang Ambon.  Semua mencit dilukai pada daerah punggung anterior sepanjang 1-1,5 cm menggunakan skalpel. Setiap hari luka diolesi dua kali dengan salep yang diuji ( salep placebo, salep komersil, dan salep ekstrak). Pengamatan patologi anatomi dilakukan setiap hari sementara pengamatan  histopatologi dilakukan pada hari ke 3, 5, 7, 14 dan 21 pasca perlukaan mencit yang dieuthanasia. Parameter pengamatan patologi anatomi adalah warna luka, pembekuan darah, terbentuknya keropeng dan ukuran luka. Parameter yang diamati pada sediaan  histopatologi adalah infiltrasi   sel-sel radang (neutrofil, limfosit, makrofag), neokapilerisasi, persentase re-epitelisasi dan ketebalan fibroblas.  Semua data kuantitatif diuji secara statistik menggunakan Analisa Sidik Ragam (ANOVA) dan dilanjutkan dengan uji wilayah berganda Duncan, sedangkan data kualitatif disajikan secara deskriptif.  Hasil penelitian menunjukkan bahwa pada pengamatan patologi anatomi kelompok salep ekstrak lebih cepat membentuk keropeng dan menutup luka tanpa bekas, jika dibandingkan dengan kontrol negatif. Hasil uji statistik pada parameter infiltrasi sel-sel radang pada kelompok salep ekstrak menunjukan hasil yang berbeda nyata (P<0.05) dengan kelompok kontrol negatif. Hasil pengamatan histopatologis menunjukkan bahwa ekstrak batang pohon pisang Ambon dalam sediaan salep mampu meningkatkan jumlah infiltrasi dari sel-sel radang, meningkatkan pembentukan neokapiler, meningkatkan persentase re-epitelisasi serta  mempercepat pembentukan fibroblas dibandingkan dengan kelompok kontrol negatif. Berdasarkan hasil diatas dapat disimpulkan bahwa pemberian salep ekstrak  batang pohon pisang ambon mempercepat proses persembuhan luka.
Daun Binahong (Andredera cordifolia Steenis) Sebagai Alternatif Insektisida Terhadap Miasis yang Disebabkan Lalat Chrysomya bezziana (ANREDERA CORDIFOLIA STEENIS (BINAHONG) LEAF AS AN ALTERNATIVE INSECTICIDE AGAINST CHRYSOMYA BEZZIANA CAUSED MYIASIS) Wientarsih, Ietje; Mustika, Aulia Andi; Wardhana, April Hari; Darmakusumah, Dodi; Sutardi, Lina Noviyanti
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.219 KB) | DOI: 10.19087/jveteriner.2017.18.1.121

Abstract

Larvae Chrysomya bezziana caused myiasis in most livestock in Indonesia. Drugs of choice for treating myasis are synthetic insecticides. Unfortunately the insecticides has negative effect on animal product, killed insect non target and insect resistenst. The research was conducted on the activity of ethanol extract of binahong leaves against various stages of C. bezziana larvae (L1, L2, and L3). Five treatments group tested were: group without any treatment (negative control), groups treated with ethanol extracts of binahong leaves 0.5%, 1%, and 2%, respectively, positive control were given coumaphos. The treatments were applied with five replications. Number of living larvae and pupae, pupae weight and number of imago were observed. The results demonstrated that 0.5% of the extract effective consentration which was able to kill the larvae (L1 and L2). Finally 2% of the extract was the most effective concentration which was able to kill larvae (L3) and decrease the pupae weight. Ethanol extract of binahong leaves was significantly able to reduce the growth of C. bezziana larvae due to contact and digestive effect of the active compounds contained in Anredera cordifolia leaf. ABSTRAK Miasis merupakan infestasi larva lalat yang terdapat pada jaringan hidup. Penyakit ini umum menyerang hewan maupun manusia. Obat yang digunakan sebagai pengendalian kasus miasis di lapangan sampai saat ini sangat terbatas dan menggunakan insektisida sintetik. Penelitian ini bertujuan melihat aktivitas ekstrak etanol daun binahong terhadap larva lalat Chrysomya bezziana pada stadium L1, L2, dan L3. Penelitian dibagi menjadi lima kelompok perlakuan dengan lima ulangan. Kelompok perlakuan terdiri dari kontrol negatif (tanpa pemberian obat), kelompok perlakuan yang diberikan ekstrak binahong dengan konsentrasi berturut-turut 0,5%, 1%, 2%, dan kelompok yang diberikan coumaphos (kontrol positif). Peubah yang diamati adalah jumlah larva yang hidup dan berkembang menjadi pupa, bobot pupa, dan daya tetas pupa menjadi imago. Hasil penelitian menunjukkan bahwa ekstrak etanol daun binahong pada konsentrasi 0,5% mempunyai efikasi sebagai larvasidal terhadap L1 dan L2 serta pada konsentrasi 2% merupakan konsentrasi terbaik sebagai larvasidal L3 larva C. bezziana. Ekstrak etanol daun binahong memiliki kemampuan menghambat larva lalat C. bezziana akibat dari efek cerna dan efek kontak bahan aktif yang terkandung di dalam daun binahong.
Infusum Daun Alpukat Sebagai Inhibitor Kristalisasi Kalsium Oksalat pada Ginjal (THE AVOCADO LEAVES INFUSUM AS INHIBITOR ON RENAL CALCIUM OXALATE CRYSTALIZATION) Madyastuti, Rini; Widodo, Setyo; Wientarsih, Ietje; Harlina, Eva
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Urine crystal is a crystal nucleus which tend to form urine stone. The case of urine stone seems to beincreased every year. Crystallization could induce acute tubular necrosis which impact on renal dysfunction.The signs of this condition are high level of urea, creatinine and decrease glomerulus filtration rate. Theobjective of this research was to evaluate the effects of infusum Persea americana Mill as an inhibitorcrystallization which induced by ethylene glycol on white male rats. 20 male rats were divided into 4groups; K1 as negative group received only distilled water ad libitum, K2 as positive group receiveddistilled water containing ethylene glycol, K3 (dose 5%) and K4 (dose 10%) as treatment groups receivedwater containing ethylene glycol and avocado leaves infusion. Phytochemsitry screening of infusion avocadoleaves consisted of flavonoid, saponin, tanine and quinone. Result of analysis showed that the level ofureum and creatinine on K2 was higher than K3 and K4 group. The increased level could be inhibited byinfusion avocado leaves. The measurement of glomerular filtration rate in treatment groups wassignificantly different (p<0.05). Descriptive histopathology observation showed that renal lesio in grouptreatment (K3 and K4) were declined. Large crystal calcium oxalate on K2 group was observed by usingpolarized microscope, whereas small crystal calcium oxalate were seen in the infusion of avocado leavesgroups. These result showed the ability of infusion of avocado leaves as an inhibitor on the growth ofcrystallization calcium oxalate