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EFFECTS OF SUSUMENO (SKIM MILK FORTIFIED WITH SOY ISOFLAVONE AND ZN SUPPLEMENT) ON MENOPAUSAL SYNDROME IN PREMENOPAUSAL WOMEN Winarsi, Hery; Muchtadi, Deddy; Zakaria, Fransiska R; Purwantara, Bambang
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 3 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Premenopause is part of natural aging process in women indicated by the decrease of ovarial estrogen production, and usually followed by development of menopausal syndrome. Susumeno, skim milk fortified with 100 mg of soy isoflavone and 8 mg of Zn was given to premenopausal women for decreasing menopausal syndrome. This research aimed to evaluate the effect of susumeno on menopausal syndrome in premenopausal women. About 33 premenopausal women aged 43-52 years old in Purwokerto were choosen as respondent. Their menopausal syndrome were identified by using questioner. The answers were scored from 1 to 5 ; i.e., 1 = not detected; 2 = less detected; 3 = mildly detected; 4 = detected; 5 = much detected. It was revealed that some of menopausal syndromes had significantly decreased, especially vaginal dryness from less detected to not detected (p=0,01); fatigue from mildly detected to not detected (p=4,07E-05); mindless from mildly detected to not detected (p=0,022); and dyspareunia from less detected to not detected (p=0,036). Key words: menopausal syndrome, soy isoflavone, Zn, premenopausal women.
Profil Lipid, Peroksidasi Lipid, dan Status Inflamatif Wanita Penderita Sindrom Metabolik Winarsi, Hery; Wijayanti, Siwi P.M.; Purwanto, Agus
Jurnal Kesehatan Masyarakat Nasional Vol. 5 No. 5 April 2011
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.299 KB) | DOI: 10.21109/kesmas.v5i5.129

Abstract

Prevalensi sindrom metabolik (SM) di Indonesia (13,13%) tergolong tinggi dengan kecenderungan terus meningkat. Salah satu akibat SM adalah disfungsi endotel, sebagai awal penyakit kardiovaskuler yang diinduksi oleh stres oksidatif dan inflamatif. Penelitian ini bertujuan untuk mengeksplorasiprofil lipid, peroksidasi lipid, dan marker inflamasi pada wanita penderita SM di Purwokerto. Sebanyak 30 wanita dengan kadar gula darah diatas normal, obesitas body mass index (BMI) > 25 kg/m2, dan berusia 40-65 tahun dilibatkan sebagai responden yang dipilih melalui survei di PoliklinikPenyakit Dalam Rumah Sakit Margono Soekarjo. Kadar kolesterol total, trigliserida, high density lipoprotein, low density lipoprotein, malondialdehid, dan plasma C-reactive protein ditentukan dalam darah responden yang mempunyai kadar gula sewaktu > 200 mg/dL. Ditemukan bahwa wanitadengan SM rata-rata berumur 50,4 tahun; BMI 31,89 kg/m2; kadar gula darah 219,4 mg/dL; kolesterol total 216,73 mg/dL; trigliserida 218,13 mg/dL; HDL 46,59 mg/dL; LDL 146,27 mg/dL; MDA 2943,4 pmol/mL; C-RP 7,62 mg/L; dan tekanan darah 153/103 mmHg. Hasil ini menunjukkan bahwapenderita SM mengalami dislipidemia disertai dengan status antioksidan rendah dan inflamasi.Kata kunci: Wanita sindrom metabolik, profil lipid, lipid peroksida, malondialdehid, C-reactive proteinAbstractPrevalence of metabolic syndrome (MS) in Indonesia (13,13%) is high and tends to increase. One of the consequences of MS is endothelial dysfunction leading to cardiovascular disease which is inducted by oxidative stressand inflammation. The aim of the present research is to explore lipid profile, lipid peroxidation, and inflammatory marker level on metabolic syndrome women in Purwokerto. Thirty women with blood glucose level greater than normal, body mass index (BMI) > 25 kg/m2, 40-65 years of age were recruited as respondent through selection by a survey in Internal Medicine Polyclinic of Margono Soekarjo Hospital in Purwokerto. In respondents with blood glucose level > 200 mg/dL, total blood cholesterol level, high density lipoprotein, low density lipoprotein, malondialdehid, and plasma C-reactive protein were determined. It was found that the MS women were 50,4 years of age; BMI 31,89 kg/m2; blood glucose 219,4 mg/dL; total cholesterol 216,73 mg/dL; triglyceride 218,13 mg/dL; HDL 46,59 mg/dL; LDL 146,27mg/dL; MDA 2943,4 pmol/mL; C-RP 7,62 mg/L; and blood pressure 153/103 mmHg. It indicates that SM women experience dyslipidemia with low antioxidant and inflammation.Key words: Metabolic syndrome women, lipid profile, peroxide lipid, malondialdehid, C-reactive protein
AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE, KATALASE, DAN GLUTATION PEROKSIDASE WANITA PENDERITA SINDROM METABOLIK Winarsi, Hery; M. Wijayanti, Siwi P.; Purwanto, Agus
Majalah Kedokteran Bandung Vol 44, No 1 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Rendahnya status antioksidan endogenus memicu perkembangan kondisi sindrom metabolik (SM). Penelitian ini bertujuan untuk mengeksplorasi aktivitas enzim antioksidan superoksida dismutase (SOD), katalase, dan glutation peroksidase (GSH-PX) dalam plasma wanita penderita sindrom metabolik. Penelitian dilakukan periode Maret?November 2010, dengan responden penelitian adalah 30 wanita yang memiliki kriteria sebagai berikut: kadar guladarah >normal, body mass index >25 kg/m2, hipertrigliseridemia, high-density lipoprotein (HDL) rendah, usia >40 tahun, dan tinggal di Purwokerto. Seleksi responden dimulai melalui survey di poliklinik internis Rumah Sakit Umum Margono Soekarjo Purwokerto. Kepada calon responden dijelaskan tentang tujuan penelitian, kondisi SM, dan perkembangannya. Calon responden yang bersedia bergabung diminta menandatangani informed consent, diukur berat badan, tinggi badan, dan tekanan darahnya. Diambil sampel darahnya 2 mL, intravena, ditentukan kadar glukosa darah, trigliserida, dan HDL. Bagian plasma, diuji aktivitas enzim SOD, katalase, dan GSH-PX. Data dipresentasikan sebagai rata-rata + standard error. Status antioksidan wanita penderita sindrom metabolik rendah, ditunjukkan oleh aktivitas SOD 379,3 Unit/mg protein, katalase 6,42 UI/mg protein, dan GSH-PX 804,9 nmol/g protein, serta kadar malondialdehid (MDA) sebesar 2.943,4 pmol/mL. Karena itu responden perlu asupan kaya antioksidan. Simpulan, status antioksidan peroksidase sama rendah yang ditunjukkan oleh rendahnya aktivitas enzim SOD, katalase, dan glutation peroksidase. [MKB. 2012;44(1):7?12].Kata kunci: Glutation peroksidase, katalase, sindrom metabolik, superoksida dismutase The Activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase Enzymes in Metabolic Syndrome WomenThe low endogenous antioxidant status induced development of metabolic syndrome (MetS) condition. The aim of this research was to explore superoxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GSH-PX) plasma activity on metabolic syndrome women plasma. Respondents were thirty women with blood glucose level >normal, body mass index >25 kg/m2, hypertrigliceridemic, low level cholesterol-high-density lipoprotein (HDL), the age >40 years and live in Purwokerto. Respondents? selection was started by a survey at internist polyclinic of Margono Soekarjo General Hospital Purwokerto. They were motivated and given an explanation about aim of the research, their diseases and development risk who were willing to be respondents were asked to sign the informed consent. Then, their body weight, height and blood pressure were measured. Their blood sample of 2 mL were taken intravenously and tested for blood glucose, triglyceride and HDL levels. Plasma was tested for the activities of SOD, Cat and GSH-PX enzymes. Presented data was mean + standard error. The antioxidant status of MetS women were low, showed on the activities of SOD, catalase, and glutathione peroxidase enzyme were 379.3 Unit/mg protein, 6.42 UI/mL, 804.9 nmol/g protein respectively, and malondialdehyde (MDA) level was 2,943.4 pmol/mL. Therefore, they need food enrich antioxidant. In conclusions, peroxidase antioxidant status was similar shown by low SOD, catalase and glutathione peroxidase enzyme activity, [MKB. 2012;44(1):7?12].Key words: Catalase, glutathione peroxidase, metabolic syndrome, superoxide dismutase DOI: http://dx.doi.org/10.15395/mkb.v44n1.75
FERMENTASI BAKTERI ASAM LAKTAT MENINGKATKAN KANDUNGAN FENOLIK DAN SERAT YOGURT SUSU KECAMBAH KACANG MERAH (PHASEOLUS VULGARIS L.), MINUMAN FUNGSIONAL UNTUK OBESITAS Winarsi, Hery
Jurnal Gizi dan Pangan Soedirman Vol 3 No 1 (2019): JURNAL GIZI DAN PANGAN SOEDIRMAN
Publisher : Program Studi Ilmu Gizi, Jurusan Kesmas Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jgps.2019.3.1.1526

Abstract

Penelitian ini bertujuan mengetahui pengaruh kadar BAL dan lama fermentasi terhadap kadar total fenol dan serat dalam yogurt susu kecambah kacang merah (Yosukamera). Kacang merah dikecambahkan 30 jam, diblender menjadi susu, kemudian difermentasi menjadi yogurt menggunakan BAL 1 dan 2%, serta lama fermentasi 12 dan 24 jam. Kadar serat meningkat oleh kadar BAL dan lama fermentasi (P<0,05), sedangkan kadar total fenol hanya dipengaruhi lama fermentasi (P<0,05). Yosukamera terbaik adalah yogurt yang dibuat dari susu kecambah kacang merah dengan kadar BAL 2% dan lama fermentasi 24 jam, mengandung antioksidan fenolik sebesar 993,08 ppm dan serat 6,29%. Yosukamera kaya antioksidan fenolik dan serat, dapat dimanfaatkan sebagai minuman fungsional bagi penderita obesitas. Sebagai selingan, yosukamera dapat dikonsumsi sebanyak 122-153 ml per harinya, dengan kandungan total fenol sebesar 121,22-152 mg dan serat 7,76-9,62 g.  
FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN KEPATUHAN ASUPAN CAIRAN DAN NATRIUM PADA PASIEN GAGAL GINJAL KRONIK (STUDI KASUS PADA PASIEN YANG MENJALANI HEMODIALISIS DI RSUD PROF. DR. MARGONO SOEKARJO PURWOKERTO) fitriani, eka; Krisnansari, Diah; Winarsi, Hery
Jurnal Gizi dan Pangan Soedirman Vol 1 No 01 (2017): Jurnal Gizi dan Pangan Soedirman
Publisher : Program Studi Ilmu Gizi, Jurusan Kesmas Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jgps.2017.1.01.344

Abstract

ABSTRACT This observational study using cross sectional design. Sampling method using purposive sampling and got 35 participants who were CKD patients undergoing hemodialysis in Prof. Dr. Margono Soekarjo Hospital, Purwokerto. The data of participant characteristic, length of time since the initiation of hemodialysis therapy, knowledge, attitude, and social support were collected by using questionnares. Patient?s compliance to fluid and natrium intake was collected by calculating fluid and natrium intake level. Data were analyzed using Fisher test. Most participants did not comply with fluid restriction, but complied with natrium restriction. All factors that have been analized had no correlation to fluid and natrium intake compliance (p > 0,05), except gender and social support factor that had correlation to fluid intake compliance (p < 0,05). Male patients needed an intensive assistance so that fluid intake compliance increased. Moreover, social support from family and others needed to achieve this goal.   Keywords: Fluid and natriumi intake compliance, gender, hemodialyss, social, support  ABSTRAK  Penelitian observasional ini menggunakan desain cross sectional. Metode pengambilan sampel menggunakan purposive sampling dan didapatkan 35 peserta penderita CKD yang menjalani hemodialisis di Rumah Sakit Prof. Dr. Margono Soekarjo, Purwokerto. Data karakteristik peserta, lamanya waktu sejak dimulainya terapi hemodialisis, pengetahuan, sikap, dan dukungan sosial dikumpulkan dengan menggunakan kuesioner. Ketaatan pasien terhadap asupan cairan dan natrium dikumpulkan dengan menghitung tingkat asupan cairan dan natrium. Data dianalisis dengan menggunakan uji Fisher. Sebagian besar peserta tidak mematuhi pembatasan cairan, namun sesuai dengan batasan natrium. Semua faktor yang telah dianalisis tidak memiliki korelasi dengan kepatuhan asupan cairan dan natrium (p> 0,05), kecuali faktor pendukung gender dan sosial yang memiliki korelasi terhadap kepatuhan asupan cairan (p <0,05). Pasien laki-laki membutuhkan bantuan intensif agar kepatuhan asupan cairan meningkat. Apalagi dukungan sosial dari keluarga dan orang lain perlu untuk mencapai tujuan ini.  Kata kunci: Kepatuhan cairan dan natrium, jenis kelamin, hemodialisis, dukungan sosial    
PRODUKSI KARIMPU DAN EFEKNYA DALAM MEMPERBAIKI PROFIL LIPID Winarsi, Hery; Amurwanto, Adi; Susilowati, Sri Sutji
Jurnal Gizi dan Pangan Soedirman Vol 1 No 01 (2017): Jurnal Gizi dan Pangan Soedirman
Publisher : Program Studi Ilmu Gizi, Jurusan Kesmas Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jgps.2017.1.01.353

Abstract

This study aims to counseling and training Karimpu production, and to know the effects of Karimpu on total cholesterol, triglycerides, LDL, and HDL plasma women with hypercholesterolemia. The trainees were 20 mothers from Ciberem village, Sumbang, Banyumas district. Karimpu drinks are composed of Chip Rimpang Cardamom and spices. As the subject of the intervention study were 30 women with hypercholesterolemia (cholesterol level > 200 mg / dl), obesity, age 45-65 years, living in Purwokerto area, willing to sign informed consent. Randomly subjects divided into 3 groups of 10 people each; I, given statin + Karimpu; II, statin + placebo; III, statin for 2 months. Blood samples were taken twice, 0 and 2 months after intervention, centrifuged, the plasma level was tested for total cholesterol, LDL, triglyceride, and HDL. People enthusiastically attended counseling and training showed test results increased 46.1%. The Karimpu product decreases cholesterol (P = 3,71E-07) and LDL (P = 0.0009), and otherwise increases HDL (P = 0.014), and retains triglyceride (P> 0.05) patients with hypercholesterolemia. Karimpu is able to inhibit the development of hypercholesterolemia towards heart disease by improving lipid profile. Keywords: Cardamom rhizome drink, lipid profile, training production.   Abstrak Penelitian ini bertujuan untuk memberikan pelatihan produksi Karimpu (minuman kaya antioksidan rimpang kapulaga) dan mengetahui efeknya terhadap kadar kolesterol total, trigliserida, LDL, dan HDL wanita penderita hiperkolesterolemia. Peserta pelatihan adalah 20 ibu-ibu dari desa Ciberem, Sumbang, Kabupaten Banyumas. Minuman Karimpu tersusun atas Chip Rimpang kapulaga dan rempah-rempah. Sebagai subjek untuk diintervensi adalah 30 wanita dengan hiperkolesterolemia, obesitas, usia 45-65 tahun, tinggal di daerah Purwokerto, dan bersedia untuk menandatangani informed consent. Subjek secara acak dibagi menjadi 3 kelompok, masing-masing 10 orang; I, diberi statin + Karimpu; II, statin + plasebo; dan III, statin, selama 2 bulan. Sampel darah diambil 2 kali, 0 dan 2 bulan setelah intervensi, lalu disentrifugasi. Bagian plasma ditentukan kadar kolesterol total, LDL, trigliserida, dan HDL. Hasilnya, peserta antusias mengikuti penyuluhan dan pelatihan produksi karimpu, ditunjukkan hasil tes meningkat 46,1%. Produk minuman menurunkan kadar total kolesterol (P = 3,71E-07) dan LDL (P = 0,0009), dan sebaliknya meningkatkan HDL (P = 0,014), dan mempertahankan trigliserida (P> 0,05). Dengan demikian, karimpu diyakini dapat menghambat perkembangan hiperkolesterolemia terhadap penyakit kardiovaskular dengan cara memperbaiki profil lipid.  Kata kunci: minuman rimpang kapulaga, profil lipid, pelatihan produksi  
EFEK LAMA PERKECAMBAHAN TERHADAP SIFAT SENSORI DAN KADAR PROTEIN TERLARUT SUSU KECAMBAH KACANG MERAH (SUKARAH) UNTUK REMAJA OBESITAS Nurjanati, Milla; Winarsi, Hery
Jurnal Gizi dan Pangan Soedirman Vol 2 No 2 (2018): Jurnal Gizi dan Pangan Soedirman
Publisher : Program Studi Ilmu Gizi, Jurusan Kesmas Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jgps.2018.2.2.1352

Abstract

This study aims to determine the effect of germination on the nature of sensory and dissolved protein content of red bean sprouts milk. This non-factorial experimental study used a randomized block design. The factors studied were germination time: 0 hours, 8 hours, 16 hours, and 24 hours. Parameters tested were soluble protein content and sensory properties including color, flavor, taste, and viscosity. Sensory test using hedonic test and hedonic quality with 50 panelist. The results were analyzed using Friedman test followed by Multiple Comparison Test at 5% level, if there is significantly different. Effectiveness index were used to determine the best product. Data were analyzed using F test at 95% confidence level. If there is significantly different continue with DMRT at 5% level.  The results was no effects of the length of time germination on soluble protein content, preferences on color, odor, and viscosity (p> 0.05). The germination period had an effect on hedonic quality in the color, flavor, taste, and thickness of product (p <0,05). The best sprout milk was for 16 hours. Thus, germination affect taste preferences, and hedonic quality in the color, flavor, taste, and viscosity of sukarah.
PEMAHAMAN MENGENAI DIET GLUTEN FREE CASEIN FREE (GFCF) SERTA PENERAPANNYA PADA ANAK AUTIS TINGKAT SEKOLAH DASAR DI SLB C YAKUT PURWOKERTO fatma, yukika; Winarsi, Hery; Purnamasari, Dyah Umiyarni
Jurnal Gizi dan Pangan Soedirman Vol 1 No 01 (2017): Jurnal Gizi dan Pangan Soedirman
Publisher : Program Studi Ilmu Gizi, Jurusan Kesmas Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jgps.2017.1.01.341

Abstract

ABSTRACT The objective of this study was to access an understanding about Gluten-Free Casein-free Diet (GFCF) and its application in autism children in special need school C Yakut Purwokerto. This study uses qualitative research methods, descriptive depth interviews with 6 key informants and 11 informants support. The results showed that the mother initially did not understand and know about the GFCF diet. After got information from therapy, physician, psychologist and school teacher, they understanding of GFCF diet with is proved by mothers already know what foods must be limited. But the mother has not been able to implement a GFCF diet in everyday life as evidenced by the child is still often eat foods made from flour and milk. This is also evidenced in the form Food Frequency Quetionnaire Qulitative. Mother's level of understanding about the GFCF diet has been good, but from the 6 main informant could not do GFCF diet properly and strictly. Keywords: Autism, Diet Gluten Free Casein Free, Flour and milk   ABSTRAK   Sekitar 60% penyandang autis mempunyai gangguan sistem pencernaan akibat alergi makanan. Gangguan sistem pencernaan ini mengakibatkan terjadinya kebocoran usus (Leaky Gut). Dampak leaky gut pada autis menyebabkan produksi hormon sekretin terhambat. Sebagai akibat, protein susu sapi (casein) dan gandum (gluten) tidak dapat dicerna dengan sempurna, karena protein tersebut tidak berubah menjadi asam amino tetapi menjadi peptida yang seharusnya dibuang lewat urin, akan tetapi pada anak autis peptida ini diserap kembali oleh tubuh dan masuk ke otak dan diubah oleh reseptor opioid menjadi morfin. Untuk itu anak autis harus membatasi asupan makanan dari gluten dan kasein, agar tidak membuat anak hiperaktif dan tantrum. Tujuan penelitian ini adalah mengetahui gambaran pemahaman ibu mengenai diet GFCF serta sejauh mana ibu menerapkan diet tersebut pada anaknya. Penelitian ini menggunakan metode penelitian kualitatif, jenis deskriptif dengan wawancara mendalam kepada 6 informan utama dan 11 informan pendukung. Hasil menunjukkan bahwa ibu pada awalnya tidak memahami dan mengetahui mengenai diet GFCF. Setelah mendapatkan informasi dari terapi, dokter, psikolog dan guru sekolah ibu menjadi paham   mengenai diet GFCF dengan dibuktikan ibu sudah mengetahui makanan apa saja yang harus dibatasi. Namun ibu belum bisa menerapkan diet GFCF di kehidupan sehari-hari yang dibuktikan dengan anak masih sering mengonsumsi makanan berbahan dasar tepung dan susu. Hal ini juga dibuktikan dalam form Food Frequency Quetionnaire (FFQ) Qulitative. Tingkat pemahaman ibu mengenai diet GFCF sudah baik, namun dari 6 informan utama belum bisa melakukan diet GFCF dengan baik dan secara ketat.  Kata Kunci : Autis, Diet Gluten Free Casein Free, tepung dan susu
KANDUNGAN PROTEIN DAN ISOFLAVON PADA KEDELAI DAN KECAMBAH KEDELAI Winarsi, Hery; Purwanto, Agus; Dwiyanti, Hidayah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2696

Abstract

The research was conducted to explore protein and isoflavone content on soy non germed and soy germed. Soybean ?slamet? was rinsed, dipped over night, drained, put in a moist room, and water sprinkled once in a while until germed. The husk of soy and soy germ were removed, and then the soy and soy germ were extracted by NaCl physiologic to become slurry. The slurry pH was arranged to 5.0, centrifugated at 10.000 rpm, and the precipitation was dryed in 70oC to obtain the soy protein and the soy germ protein flour. The levels of protein and isoflavone flour were determined. The result showed that protein content of soy was 36.5%, while protein of soy germ was 42%. The isoflavone of soy protein was 26.7 ppm, consisting of 11.5 ppm genistein, 10.2 ppm daidzein and 5 ppm glicitein, but the soy germ protein was 39.1 ppm, consisting of 14.6 ppm genistein, 16.9 ppm daidzein and 7.6 ppm glicitein. The conclusion is that germed process could increase protein and isoflavone content. The soy protein isoflavone was dominated by genistein and daidzein, while soy germ protein isoflavone was dominated by daidzein and glicitein.
EFFECTS OF SOY-GERM PROTEIN ON CATALASE ACTIVITY OF PLASMA AND ERYTHOCYTE OF METABOLIC SYNDROME WOMEN WINARSI, HERY; WIJAYANTI, SIWI PRAMATAMA MARS; SASONGKO, NURTJAHJO DWI; PURWANTO, AGUS
HAYATI Journal of Biosciences Vol. 22 No. 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (949.131 KB) | DOI: 10.4308/hjb.22.1.1

Abstract

Oxidative stress always accompany patients with metabolic syndrome (MS). Several researchers reported that soy-protein is able to decrease oxidative stress level. However, there is no report so far about soy-germ protein in relation to its potential to the decrease oxidative stress level of MS patients. The aim of this study was to explore the potential of soy-germ protein on activity of catalase enzyme in blood?s plasma as well as erythrocytes of MS patients. Double-blind randomized clinical trial was used as an experimental study. Thirty respondents were included in this study with MS, normal level blood sugar, low-HDL cholesterol but high in triglyceride, 40-65 years old, Body Mass Index > 25 kg/m2, live in Purwokerto and agreed to sign the informed consent. They were randomly grouped into 3 different groups, 10 each: Group I, was given special milk that contains soy-germ protein and Zn; Group II, soy-germ protein, while Group III was placebo; for two consecutive months. Data were taken from blood samples in 3 different periods i.e. 0, 1, and 2 months after treatment. Two months after treatment, there was an increase from 5.36 to 20.17 IU/mg (P = 0.028) in activity of catalase enzyme in blood?s plasma respondents who consumed milk containing soy-germ protein with or without Zn. A similar trend of catalase activity, but at a lower level, was also noticed in erythrocyte; which increased from 88.31 to 201.11 IU/mg (P = 0.013). The increase in activity of catalase enzyme in blood?s plasma was 2.2 times higher than that in erythrocytes.