Adi Winarto
Departemen Anatomi Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor

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STUDI MIKROANATOMI PANKREAS KODOK LEMBU MENGGUNAKAN METODE PEWARNAAN BAKU DAN IMMUNOHISTOKIMIA (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) Adnyane, I Ketut Mudite; ., Supratikno; Winarto, Adi; Agungpriyono, Srihadi
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.
PAT-7 A NATURAL CASE LIKE DIABETES RAT AS A NEW APPROACH ON UNDERSTANDING ALERT OF GLUCOSE METHABOLISM Winarto, Adi; Komariah, Komariah; Bambang, Kiranadi; Wasmen, Manalu; Handharyani, Ekowati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Currently, diabetic case as a degenerative disease has widely known that it can be cure by insulin.  A remain puzzle is proliferation do not found in rest Beta cells, so insulin injection has to be applied entire life of diabetic patient. So far, there is no diagnostic approach that expresses such of thing which may indicate an early anomaly on glucose metabolism process. The aim of this research to get case model of hyperglycemic rat that has long period in process.
ANATOMI ORGAN REPRODUKSI JANTAN BIAWAK AIR ASIA, VARANUS SALVATOR (REPTIL: VARANIDAE) Mahfud, .; Nisa?, Chairun; Winarto, Adi
Acta VETERINARIA Indonesiana Vol. 3 No. 1 (2015): Januari 2015
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (587.266 KB) | DOI: 10.29244/avi.3.1.1-7

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Indonesia merupakan negara dengan tingkat eksploitasi biawak V. salvator terbesar di dunia yang sebagian besar untuk melayani permintaan perdagangan kulit. Tingginya permintaan kulit biawak di Indonesia mengkhawatirkan menyebabkan turunnya populasi satwa tersebut. Penelitian ini bertujuan untuk mempelajari anatomi organ reproduksi jantan biawak air asia (Varanus salvator) (Reptil: Varanidae). Dua ekor biawak jantan dewasa digunakan dalam penelitian ini. Hewan dianestesi, dilakukan exanguinasi, dan difiksasi dengan larutan paraformaldehida 4% secara perfusi. Pengamatan dilakukan terhadap situs viscerum, morfologi, dan morfometri organ reproduksi mulai testis sampai hemipenis. Secara makroskopis, organ reproduksi jantan V. salvator terdiri atas testis, ductus epididymidis, ductus deferens dan hemipenis yang masing-masing berjumlah sepasang. Posisi testis menggantung di dinding dorsal coelom melalui mesorchium. Dari bagian dorsal testis terdapat ductus epididymidis yang panjangsampai di ujung kaudal ginjal. Ductus deferens, berupa saluran kecil, lurus dan berakhir di ujung hemipenis yang terletak di dalam pangkal ekor. Pada bagian kranial hemipenis ditutupi oleh papillae berbentuk konikal. Di kaudal dari hemipenis ditemukan otot retraktor yang memanjang ke arah ekor, dan diduga berperan menarik hemipenis ke dalam setelah kopulasi. Organ reproduksi jantan biawak secara umum mirip dengan reptilia lain khususnya ular dan kadal, dengan karakteristik adanya sepasang hemipenis.Kata kunci: Varanidae, Varanus salvator, organ reproduksi jantan, hemipenis, otot retraktor. (Anatomy of The Male Reproductive Organ of Water Monitor, Varanus salvator (Reptil: Varanidae))Indonesia is a country with high levels of exploitation of Varanus salvator that mainly serve the demand of leather trade. The high demand of lizard leather in Indonesian was alarming, cause a decline population of these animals. To improve our understanding on reproduction organs of the animal, we conduct this anatomical study. The study was used two adult male lizards. The animals were anesthetized, exanguinated and fixated in 4% paraformaldehyde by tissue perfusion method. Observations were performed to the visceral site, morphological and morphometrical of the male reproductive organs, from testes to hemipenes. Macroscopically, male reproductive organs of V. salvator were a pair of testes, epididymidis ducts, deferens ducts and hemipenes. The testis attached to dorsal wall of the coelom and fixed by the mesorchium. The epididymidis duct was long tubes that located in the dorsal of testes, winding up at the caudal end of the kidney. The deferens duct was a small duct, running straight and last at the end of each hemipenis, located at the base of the tail. The cranial part of each hemipenis was covered by conical shaped papillae. Furthermore, at the caudal of each hemipenis was found the retractor muscle that extends toward the tail, and is thought to contribute to the retracting hemipenis after copulation. The male reproductive organs of V. salvator are generally similar to the other reptiles, especially snakes and lizards, with peculiar a pair of hemipenes.Keywords: Varanidae, Varanus salvator, male reproductive organs, hemipenes, retractor muscles.
VALIDASI METODE IN VITRO DALAM MEMPERKIRAKAN PENGKATEGORIAN INDEKS GLIKEMIK IN VIVO Nurhayati, Aprinia Dian; Rimbawan, Rimbawan; Anwar, Faisal; Winarto, Adi
Indonesian Journal of Human Nutrition Vol 6, No 2 (2019)
Publisher : Jurusan Gizi, Fakultas Kedokteran, Universitas Brawijaya Malang

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ABSTRACTThe use of in vitro methods that mimic carbohydrate digestion in the gastrointestinal was expected to be able to predict glycemic response in human in a simple, faster and relatively inexpensive way. Englyst et al. (2003) and Argyri et al. (2016) became the selected in vitro method that tested their validity in this study by determine their ability to classify food according to the results of in vivo glycemic index (GI). The test uses rice with different GI categories (low, medium and high). Six rice varieties were cooked using a rice cooker then tested for proximate analysis (protein, fat, water, ash, carbohydrate), total dietary fiber, amylose, amylopectin and starch. A total of 20 subjects who met the inclusion criteria were divided into two comparable groups to measure their blood glucose levels for 2 hours after consuming test food. Cirebon black rice, Cisokan and Inpara 5 have moderate GI value, while Inpari 24, Sintanur and Grendel glutinous rice were classified as high GI value. Glucose levels of six rice varieties as measured by Englyst et al. (2003) at 20th minute and Argyri et al. (2016) at 120th minute has a comparable rank with in vivo glycemic index. Therefore, both methods are suitable for estimating GI value in rice samples.ABSTRAKPenggunaan metode in vitro yang meniru pencernaan karbohidrat dalam gastrointestinal diharapkan dapat memprediksi respon glikemik in vivo secara lebih sederhana, cepat dan relatif murah. Metode Englyst et al. (2003) dan Argyri et al. (2016) menjadi metode in vitro terpilih yang diuji validitasnya dalam penelitian ini untuk mengetahui kemampuan keduanya dalam menggolongkan pangan sesuai hasil pengujian indeks glikemik (IG) in vivo. Pengujian menggunakan nasi dengan kategori IG berbeda (rendah, sedang dan tinggi). Sebanyak enam varietas beras dimasak menggunakan rice cooker kemudian nasi diuji kadar proksimat (protein, lemak, air, abu, karbohidrat), serat pangan total, amilosa, amilopektin dan pati. Sebanyak 20 orang yang memenuhi kriteria inklusi dibagi dalam dua kelompok sebanding untuk diukur kadar gula darahnya selama 2 jam setelah mengonsumsi makanan. Nasi hitam Cirebon, Cisokan dan Inpara 5 memiliki nilai IG sedang, sedangkan nasi Inpari 24, Sintanur dan ketan Grendel tergolong berindeks glikemik tinggi. Kadar glukosa nasi yang diukur dengan metode Englyst et al. (2003) menit ke-20 dan metode Argyri et al. (2016) menit ke-120 apabila dibuat pemeringkatan terendah hingga tertinggi menunjukkan urutan peringkat yang sesuai dengan urutan nilai indeks glikemik in vivo. Oleh karena itu, keduanya menjadi metode yang sesuai untuk memperkirakan nilai indeks glikemik in vivo pada sampel nasi.
CHANGES IN UTERINE CAPABILITY DUE TO INCREASED LITTER SIZE AT 7 WEEKS OF PREGNANCY IN KACANG GOAT Arif, Ridi; Satrija, Fadjar; Winarto, Adi; Boediono, Arief; Manalu, Wasmen
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.6558

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The purpose of this research was to study the changes in uterine support capability power due to increase of litter size in Kacang goat. In this study, 9 pregnant female Kacang goats were divided into 4 different groups based on litter size, namely 1, 2, 3, and 4. At 7 weeks of pregnancy, the experimental Kacang goats were sacrificed to observe the macro and micro parameters of the uterus. The results showed that the litter size had a quadratic relationship with micro parameters of uterus but it had a linear relationship with macro parameters of the uterus. The variables of uterine glands area, glands lumen area, and cytoplasmic area reached maximum condition at the litter size of 2.5. Litter size had a linear relationship with the volume, weight, and dimension of the uterus. It can be concluded that the optimal number of litter size in Kacang goat was two offsprings which was proven by the optimal function of the uterus to support fetal development.
VIABILITAS OOSIT DOMBA PASCATRANSPLANTASI OVARIUM DOMBA DALAM UTERUS KELINCI PSEUDOPREGNANT Sumarmin, Ramadhan; Winarto, Adi; Yusuf, Tutty Laswardi; Boediono, Arief
Majalah Ilmiah Peternakan Vol 11 No 1 (2008)
Publisher : Fakultas Peternakan Universitas Udayana

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ABSTRAK Telah dilakukan penelitian untuk mengetahui viabilitas oosit domba yang dikoleksi dari ovarium domba pascatransplantasi intrauterus pada kelinci pseudopregnant. Ovarium domba ditransplantasikan dalam uterus kelinci pseudopregnant pada hari pertama bunting semu dan kemudian diambil kembali setelah lima (P5) atau tujuh (P7) hari transplantasi. Sebagai kontrol digunakan ovarium segar. Oosit dikoleksi dari ovarium pascatransplantasi dengan metode slicing (pencacahan) di dalam media phosphate buffer saline (PBS) yang disuplementasi dengan 5% fetal bovine serum (FBS) dan 100 IU penicillin-streptomisin/ml. Oosit hasil koleksi selanjutnya dimaturasi dalam Tissue Culture Medium (TCM)-199 yang disuplementasi dengan 10% FBS, 10 IU follicle stimulating hormone (FSH)/ml dan 100 IU penicillin-streptomisin/ml. Oosit selanjutnya diinkubasi dalam inkubator CO2, dengan kandungan CO2 5% dan suhu 38ºC, selama 24 jam. Setelah dimaturasi, oosit diwarnai dengan aceto-orcein 2% untuk menentukan status inti oosit. Hasilnya memperlihatkan bahwa oosit yang mampu mencapai tahapan perkembangan dengan status inti M-II setelah maturasi pada P5 (35,05%) dan P7 (35,24%) nyata lebih sedikit (p<0,05) dibandingkan dengan kontrol (56,65%). Dapat disimpulkan bahwa viabilitas oosit domba pascatransplantasi di dalam uterus kelinci pseudopregnant masih ditemukan meskipun persentasenya lebih rendah. EWE OOCYTE VIABILITY FROM EWE OVARIAN AFTER INTRAUTERINE TRANSPLANTATION TO PSEUDOPREGNANT RABBIT ABSTRACT The aim of the present study was to investigate the ewe oocyte viability from ewe ovary after intrauterine transplantation to pseudopregnant rabbit. The ewe ovary was transplanted intrauterine on day 1 to pseudopregnant rabbit and oocytes recollected on five (P5) or seven (P7) days posttransplantation. The fresh ovary was used as the control. The oocytes were collected from the ovaries by slicing method in Phosphate Buffer Saline (PBS) supplemented with 5% of Fetal Bovine Serum (FBS), and 100 IU/ml of penicillin-streptomycin. Oocytes were matured in Tissue Culture Medium (TCM)-199 supplemented with 10% FBS, 10 IU/ml of Follicle Stimulating Hormone (FSH), and 100 IU/ml of penicillin-streptomycin. Oocytes were incubated in CO2 incubator with 5% CO2, 38°C for 24 h. After maturation, the oocytes were stained with 2% aceto-orcein to determine the nuclear oocytes status. The result showed that the oocytes could reach the M-II phase from P5 (35.05%) and P7 (35.24%) decreased significantly (p<0.05) compared to the control (56.65%). However it can be concluded that the oocytes viability still preserved intrauterine in pseudopregnant rabbit.
KAJIAN IN VITRO AKTIVITAS SEL-SEL TROFOBLAS BLASTOSIS MENCIT AGING DAN PENGARUHNYA TERHADAP KEGAGALAN IMPLANTASI Djuwita, Ita; Helmita, Roza; Winarto, Adi; -, Wahyudin
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P<0,05). It can be concluded that the impairment of blastocysts implantation especially, in agingmice were caused by the low activity of the NADH-CoQ reductase that play important role in energyproduction needed for the hatching and trophoblast outgrowth.
THE PROFILE OF SUPEROXIDA DISMUTASE AND MALONDIALDEYDE LEVEL IN THE LIVER TISSUE OF HYPERCHOLESTEROLEMIC RATS TREATED WITH HOLOTHURIA NOBILIS POLYSACCHARIDE Ulhusna, Fitrah Asma; Winarto, Adi; Wresdiyati, Tutik
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i2.13189

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The aim of this research was to analyze the profile of superoxide dismutase (SOD) and malondialdehyde (MDA) on the liver tissue of hypercholesterolemic rats which were given Holothuria nobilis polysaccharides (HNP). A total of 15 male rats strain Sprague Dawley were divided into prevention and curative groups. Prevention group consisted of negative/non-hypercholesterolemic group (K-), positive/hypercholesterolemic group (K+), and hypercholesterolemic prevention group which were given 1% cholesterol diet and HNP at dose of 400 mg/kg bw (PCh). The treatments were given for 28 days. The curative group was consisted of the hypercholesterolemic group, which was given 1% cholesterol diet for 28 days, then followed by standard diet for 28 days (Ch), and the hypercholesterolemia curative group which was given 1% cholesterol diet for 28 days, then followed by 400 mg/kg bw HNP for 28 days (ChP). The antioxidant activity of HNP was analyzed by DPPH method. At the end of study the liver tissue was collected and analyzed for MDA, SOD while Cu,Zn-SOD was analyzed by immunohistochemical technique. The results showed that the antioxidant activity of HNP was weak. The MDA level (µg/g) in K-, K+, PCh, Ch, and ChP groups were 1.19±0.6; 3.37±0.79; 0.29±0.14; 9.11±0.72; and 3.14±1.06, respectively. The SOD activities (U/g) in K-, K+, PCh, Ch, and ChP groups were 2141.11±83.88; 1541±211.69; 2096.67±166.66; 1063.33±88.19; 1685.55±167.77, respectively. The immuno reactivity of Cu,Zn-SOD showed that HNP could increase Cu,Zn-SOD in the liver tissues of both groups. This study concluded that the HNP increased SOD activity, Cu,Zn-SOD antioxidant content, and decreased MDA levels in the liver tissues of hypercholesterolemic rats in both preventive and curative groups.
PROLIFERASI DAN DIFERENSIASI SEL TULANG TIKUS DALAM MEDIUM KULTUR IN VITRO YANG MENGANDUNG EKSTRAK BATANG CISSUS QUADRANGULA SALISB. (SIPATAH-PATAH) Djuwita, Ita; Pratiwi, Irma Amalia; Winarto, Adi; Sabri, Mustafa
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.295

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Penelitian mengenai pengaruh ekstrak batang Cissus quadrangula Salisb. (sipatah-patah) terhadap tingkat proliferasi dan diferensiasi sel-sel tulang tikus (Sprague Dawley) prepuber umur empat minggu menggunakan sistem kultur in vitro. Sel-sel tulang dikultur dalam medium dulbecco?s modified eagle?s medium (DMEM) yang diberi tambahan newborn calf serum (NBCS) 10%, non essential amino acid (NEAA) 10%, NaHCO3, ITS 1 µl/ml (mengandung insulin 5 ?g/ml, transferin 10 ?g/ml, selenium 5 ?g/ml; Sigma St Louis USA), dan 50 µg/ml gentamisin (mDMEM), dengan dan tanpa ekstrak Cissus quadrangula (CQ). Penelitian terdiri atas lima perlakuan yakni kontrol positif (mDMEM + deksametason 10-8 M), kontrol negatif (mDMEM), dan tiga konsentrasi CQ: mDMEM + CQ 0,3 mg/ml; mDMEM + CQ 0,6 mg/ml; dan mDMEM + CQ 1,2 mg/ml. Kultur dilakukan dalam inkubator CO2 5%, pada suhu 37° C selama tujuh hari. Parameter yang diamati adalah konsentrasi sel, komposisi, diameter osteoblas, dan diameter osteosit. Konsentrasi sel dihitung menggunakan hemositometer Newbauer. Komposisi osteoblas dan osteosit ditentukan berdasarkan pengamatan morfologi di bawah mikroskop cahaya. Diameter sel diukur menggunakan eyepiece micrometer. Data dianalisis menggunakan analisis varians dan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak Cissus quadrangula Salisb. pada konsentrasi 0,3 mg/ml; 0,6 mg/ml; dan 1,2 mg/ml secara signifikan dapat meningkatkan proliferasi sel tulang; dan pada konsentrasi 0,6 mg/ml mampu menginduksi diferensiasi osteoblas menjadi osteosit (P 0,05). Disimpulkan bahwa pemberian ekstrak Cissus quadrangula pada konsentrasi 0,6 mg/ml ke dalam medium kultur dapat meningkatkan proliferasi dan diferensiasi osteoblas.
MIKROMORFOLOGI ALAT KELAMIN PRIMER BIAWAK AIR (VARANUS SALVATOR BIVITTATUS) JANTAN (MICROMORPHOLOGICAL STRUCTURE OF PRIMARY REPRODUCTIVE ORGAN OF MALE WATER MONITOR LIZARD (VARANUS SALVATOR BIVITTATUS)) M, Mahfud; Winarto, Adi; Nisa, Chairun
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3375

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The study aims to determine the histological structure of the testes, ductus epididymidis and ductus deferens of Varanus salvator bivittatus. The tissue samples were obtained by the histological techniques preparation with thickness section is 3-4 µm then stained using hematoxylin-eosin (HE) and Masson's Trichrome (MT). The result showed that testes are covered by tunica vaginalis and tunica albuginea (tunica fibrous and tunica vasculosa). Tubulus seminiferous as a parenchyma are found in lobules of testes which are formed by septum as extension of tunica albuginea. Tubulus seminiferous is composed by layers of germinal epithelium cells consist of spermatogenia, Sertoli cell, spermatocyte and spermatid which is developed further to be spermatozoa in the lumen of tubulus. Among the tubulus seminiferous, there are groups of epithelioid cells called Leydig cells. The end of tubulus seminiferous formed ductus epididymidis which then ended up and known as ductus deferens. Epididymis is covered by dense connective tissue. Ductus epididymidis is divided into three segments: cranial, medial and caudal. It is lined by pseudostratified columnar epithelium which is varied in its thickness, amount and diameter of lumen. Lumen of the ductus deferens was lined by pseudostratified ciliated columnar epithelium and thin muscularis mucosal layer and thick circular smooth muscle were covered externally.Key words: Varanus salvator bivittatus, testes, ductus epididymidis, ductus deferens, micromorphology