Ni Nyoman Wirasiti
Kelompok Studi Ekowisata, Jurusan Biologi, Fakultas MIPA, Universitas Udayana.Denpasar-Bali

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RESPON MORFOLOGIS DAN EKSPRESI GEN AQUAPORIN PADA PADI IR 64 YANG MENGALAMI CEKAMAN KEKERINGAN PADA FASE REPRODUKTIF (MORPHOLOGICAL RESPONSES AND AQUAPORIN GENE EXPRESSION IN RICE IR64 UNDER DROUGHT STRESS AT THE REPRODUCTIVE STAGE) Pharmawati, Made; Wirasiti, Ni Nyoman; Wrasiati, Luh Putu
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18579

Abstract

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice
VARIASI BENTUK WAJAH LAKI-LAKI DAN PEREMPUAN DARI DESA PANGLIPURAN, KABUPATEN BANGLI, PROVINSI BALI PUTRA, AGUNG EKO CAHYA KUSUMA; WIRASITI, NI NYOMAN; SETYAWATI, IRIANI
Jurnal Biologi Udayana Vol 21 No 2 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.277 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i02.p03

Abstract

This research determined male and female face type of the adults in Panglipuran adults. The analysis of face types Geometric Morphometric Technique in thin-plate-splin (tps) program. Adults female and 14 male aged 25-35 years old were photographed for both frontal and lateral faces with a digital camera Canon EOS 60D. Landmark data was processed by photo processor program tpsDig version 2.04, tpsSuper version 1.13, tpsRelw version 1.42, tpsSplin 1.20 and both of ape and gee package of R Program version 3.2.5. The average of male?s face shape type in Panglipuran village was ellipse and round for female?s. We found 2 frontal and 2 lateral for male?s face variation, we also found 3 frontal and 3 lateral for female?s. Frontal face of male and female that showed high variation included right subaurale, left subaurale, right gonion, left gonion, and gnathion. Lateral face of male and female that showed high variation were trichion, frontozygomaticus, superciliare, superaurale, pogonion, glabella, the minimum point of the curve below earlobe, gonion and gnathion.
RAGAM ALEL DNA MITOKONDRIA MASYARAKAT SOROH PANDE DI BALI DENGAN METODE PCR-RFLP Septiasari, Ni Putu Senshi; Junitha, I Ketut; Wirasiti, Ni Nyoman
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.04 KB) | DOI: 10.24843/metamorfosa.2017.v04.i02.p12

Abstract

Masyarakat Bali mengelompokkan diri berdasarkan soroh atau garis keturunan tetapi bukti genetik pengelompokkan tersebut belum diketahui. Salah satu soroh/ klan yang memiliki persebaran yang luas di seluruh Bali adalah soroh/ klan Pande. Penelitian ini dilakukan untuk mengetahui ragam alel DNA mitokondria masyarakat soroh Pande di Bali dengan metode Polymerase Chain Reaction-Fragment Length Polymorphism (PCR-RFLP) yang berguna untuk kepentingan forensik. Sampel sel epitel mukosa mulut diambil dengan cara purposive sampling yang terdiri dari 35 sampel warga Pande. Tahapan penelitian meliputi ekstraksi DNA dengan metode fenol-kloroform yang telah dimodifikasi, amplifikasi DNA pada daerah D-loop DNA mitokondria (mtDNA), digesti produk PCR dengan lima macam enzim restriksi dan elektroforesis dengan menggunakan gel agarose 1 % dan PAGE 10%. Penelitian ini mendapatkan sembilan ragam alel. Digesti dengan enzim Hae III, Hinf I, Mbo I dan Dde I mendapatkan masing-masing dua tipe pemotongan sedangkan enzim Hind III tidak memiliki situs pengenalan pada daerah D-loop DNA mitokondria. Enzim Hind III tidak dapat digunakan untuk membedakan profil DNA antar individu karena bersifat monomorfik.
PELESTARIAN FLORA DI LINGKUNGAN PURA TAMAN AYUN MENUNJANG ATRAKSI NOMINASI WARISAN BUDAYA DUNIA Wirasiti, Ni Nyoman; Ginantra, I Ketut; Dalem, A.A.G. Raka; Muksin, I Ketut
Bumi Lestari Journal of Environment Vol 11 No 1 (2011)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.416 KB)

Abstract

Taman Ayun is a tourist destination which is proposed as a world cultural heritage. Thus, the data about Taman Ayun are important things to be collected to support the proposal. This study is expected to provide data on flora. Data on flora were gathered based on site observation on September 5, 2008. The data collection was also supported by other data collecting techniques such as interview and literature review. Result of the study showed that there were 125 plant species around the temple. As much as 16,8 % were classified as rare/threatened or endangered, 1,6% rare and protected, and the rest (81,6 %) were not protected and not rare species. The highest proportion of the plant (30 %) were decorative plant. Others were material for ceremony (upakara) (21%), food and vegetables (19 %). All of those were covering 70 % of the plant. The rest were for medicine, providing shaded spots, animal fodder, other use and has not been used.
JENIS DAN MANFAAT FLORA PULAU DERAWAN KABUPATEN BARAU PROVINSI KALIMANTAN TIMUR Darsini, Ni Nyoman; Sugi Wahyuni, I .G. A.; Darmadi, A.A.K.; Wirasiti, Ni Nyoman
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on identication of plants species available on Derawan Island, Berau Regency, East Kalimantan has been conducted based on exploration survey around the island between 2002-2005. The plants whicht have observed were then listed on a table, their scientific names wrre recorded, their status (whether being protected or not) were determined, and the benefits or use of the plants were also listed. From this study it can be found 45 species of 30 families. They may bring benefits for the locals as vegetables, source of medicine, providing shady spots, or decorative plants.
VARIASI POLA SIDIK JARI SOROH BRAHMANA SIWA DI BALI Bajing Agastya, Ida Bagus; Junitha, I Ketut; Wirasiti, Ni Nyoman
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.289 KB) | DOI: 10.24843/JSIMBIOSIS.2017.v05.i02.p01

Abstract

ABSTACTResearch was conducted to determine the patterns of Brahmana Shiva fingerprint in Bali. The fingerprint samplesas many as 240 people, which consist of 120 men and 120 women from four generation of Brahmana Shiva in Bali. Thefingerprints were collected using a fingerprint pad then the data were analyzed using Chi?Square test, Student-t test,Dankmerijer index and Furuhata index. The result of fingerprints examination shown that there were four fingerprintspattern i.e. arch (A), loop ulnar (LU), loop radial (LR), and whorl (W) from four sub clan (Brahmana Manuaba, Kemenuh,Keniten, Mas), with different frequencies and anequal distribution from each fingers and both hands. The arch (A) patternin clan Brahmana Manuaba and Kemenuh have the same 0.83 % frequencies, while the frequencies of Keniten 1.17 % andMas 1.67 %. The frequencies of Loop ulnar (LU) pattern from clan Brahmana Mas were 42.66 %, Manuaba 36 %, Keniten39 %, and Kemenuh 34.33 %. The frequency of loop radial (LR) pattern from clan Brahmana Manuaba were 42.17 %,Keniten 41.83 %, Mas 37.83 %, and Kemenuh 29.51 %. The frequencies of whorl (W) pattern from sub clan BrahmanaKemenuh, Manuaba, Keniten, and Mas were 35.33 %, 22 %, 18 %, and 17.83 %, respectively. The result show asignificant differences between sub clan Mas and Kemenuh using Dankmerijer Index (DI). The result show a significantdifferences between sub clan Kemenuh with Mas, Keniten and Manuaba using Furuhata Index (FI). Total Ridge Countbetween men and women of Brahmana Shiva offspring showed unsignificantly diferences at level 5%. However, there wasa significant difference of Total Ridge Count from sub clan Manuaba with Mas, Kemenuh, and Keniten.Keywords : fingerprints, arch, loop, whorl, clan brahmana shiva.
PENELITIAN PENDAHULUAN VARIASI GENETIK MASYARAKAT SOROH PANDE BERDASARKAN PENANDA DNA MIKROSATELIT KROMOSOM Y: MASYARAKAT SOROH PANDE DESA ABIANSEMAL, BADUNG Wulandari, Ni Putu Putri; Junitha, I Ketut; Wirasiti, Ni Nyoman
Jurnal Biologi Udayana Vol 18 No 1 (2014): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Masyarakat Hindu di Bali dibedakan ke dalam kelompok-kelompok berdasarkan garis keturunan laki-laki yang disebut klan atau di Bali lebih dikenal dengan istilah soroh. Salah satu soroh yang dikenal di Bali adalah soroh Pande. Penelitian ini bertujuan untuk mengetahui variasi genetik masyarakat soroh Pande di Desa Abiansemal, Badung menggunakan empat lokus mikrosatelit kromosom Y, yaitu DYS19, DYS390, DYS393 dan DYS395. Berdasarkan hasil analisis, didapatkan 11 ragam alel dan enam haplotipe yang didominasi oleh haplotipe A (200, 215, 121, 123) dengan frekuensi sebesar 0,65 serta keragaman genetik sebesar 0,32 ± 0,07. Ragam haplotipe yang didapatkan menunjukkan bahwa masyarakat soroh Pande di Desa Abiansemal berasal dari berbagai sumber gen.