Articles

Tanggap Fisiologi Akar Sorgum (Sorghum bicolor L. Moench) terhadap Cekaman Aluminium dan Defi siensi Fosfor di dalam Rhizotron Agustina, Karlin; Sopandie, Didy; Trikoesoemaningtyas, ,; Wirnas, Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.451 KB) | DOI: 10.24831/jai.v38i2.1791

Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> A study on physiological mechanism of sorghum to aluminum (Al) toxicity and phosphorous (P) defi ciencies was conducted using several sorghum genotypes in rhizotron. The study was conducted in the greenhouse of the University Farm, Bogor Agricultural University from August to October 2009. The experiment was carried out as a Factorial experiment in a Completely Randomized Design with three replications. The fi rst factor was sorghum genotypes consisted of Numbu (tolerant) and B-75 (sensitive), and the second factor was combination of lime and phosphorous fertilization consisted of: no lime-no P (R1), no lime-low P (R2), no lime-suffi cient P (R3), lime-no P (R4), lime-low P (R5) and lime-suffi cient P (R6). The results showed that all variables were signifi cantly affected by the interaction of media and genotype, except for length of shoot and total P uptake. Under low P and Al toxicity, sorghum variety Numbu showed a higher nutrient effi ciency than B-75 with ability of forming greater dry mass. The sensitive genotypes showed a higher nutrient uptake but a lower P nutrient use efficiency under Al toxicity and P defi ciency. Total P uptake and P nutrient use effi ciency were highly correlated with root growth and dry material accumulation. Keywords: sorghum, Al toxicity, P defi ciencies, P nutrient use effi ciency, P uptake
Anther Culture Ability from Crossess Between Upland and New Plant Types of Rice Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari S.; Abdullah, Buang; Safitri, Heni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.417 KB) | DOI: 10.24831/jai.v38i2.1790

Abstract

Anther culture provides rapid route in obtaining pure lines in a single generation through producing green haploid plants that may be spontaneously doubled. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of eight F1s derived from crossess between upland and new plant types of rice and from their four parents through anther culture. Completely randomized design with 25 replications was used in this research. Treatments consisted of four parent lines/varieties i.e. P1 (Fatmawati and BP360E-MR-79-2), P2 (Fulan Telo Gawa and Fulan Telo Mihat) and eight F1s obtained from reciprocal crosses of P1 and P2. Callus induction medium was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1  kinetin + 10-3  M Putrescine, while regeneration medium was based on MS + 0.5 mg L-1  NAA + 2.0 mg L-1  kinetin + 10-3  M Putrescine. The result indicated that F1 derived from Fatmawati x Fulan Telo Gawa (5.00% green plants per total anther) and their reciprocal (3.80% green plants per total anther) crosses were the most responsive genotypes in rice anther culture (had high anther culture ability). The F1 genotypes were more effective to produce green and doubled haploid plants in rice anther culture than their parents. From this research, 161 double haploid plants (29.81%) from total acclimated green plantlets were obtained.  Keywords: anther culture, upland rice, new plant type of rice
Perbedaan Tanggap Morfologi Akar Galur Inbrida Sorgum pada Kondisi P Rendah Putri Andini Mandasari; Wirnas, Desta; Trikoesoemaningtyas; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v48i1.29894

Abstract

Akar tanaman memiliki respon yang bervariasi sebagai bentuk adaptasi pada kondisi P rendah. Penelitian ini bertujuan untuk memperoleh informasi tanggap morfologi akar dari galur-galur inbrida sorgum pada kondisi P rendah. Percobaan ini menggunakan rancangan kelompok lengkap teracak (RKLT) tersarang dua faktor yaitu genotipe dan dosis pupuk P. Genotipe yang digunakan terdiri atas 11 galur inbrida sorgum (RIL-F8), tetua peka (B-69), tetua toleran (Numbu) dan 2 varietas nasional (WHP dan Kawali). Dosis pupuk yang digunakan terdiri atas dosis P cukup (66.76 kg ha-1 P2O5) dan P rendah (33.38 kg ha-1 P2O5). Hasil penelitian menunjukkan bahwa terdapat perbedaan tanggap morfologi akar di antara galur-galur inbrida (RIL-F8) sorgum pada kondisi P rendah. Galur-galur toleran memiliki panjang akar terpanjang (41.33-61.87 cm), volume akar (8.87-12.87 mL), diameter akar terpanjang (0.88-1.02 mm) dan jumlah akar proteoid (2.4-4.0) yang lebih tinggi dibandingkan galur-galur peka pada kondisi P rendah. Karakter-karakter tersebut mendukung tingginya biomassa galur toleran pada kondisi P rendah, sehingga menjadi karakter penting untuk seleksi adaptasi akar pada kondisi P rendah. Kata kunci: akar proteoid, biomassa, fosfor, toleran
Identifikasi Aksi Gen Epistasis pada Toleransi Kedelai terhadap Cekaman Aluminium Sihaloho, Arvita Netti; ., Trikoesoemaningtyas; Sopandie, Didy; Wirnas, Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.275 KB) | DOI: 10.24831/jai.v43i1.9587

Abstract

The efectiveness of selection depends on the gene action cotrolling the character.  The presence of epistatic gene action will delay the genetic gain in self pollinated crops like soybean. The objective of this research was to obtain information on genetic control of soybean tolerance to aluminum toxicity on nutrient culture. This research was conducted at green house of  the University Farm IPB Cikabayan, Bogor, from April to June 2013. This research used 120 seedlings of F2 population  generated from  crossing between Argomulyo (Al-sensitive) with Tanggamus (Al-tolerant). Seedlings were grown in nutrient culture for 14 days with Al concentration of 1.5 mM and pH 4.0, to observe the vegetative growth under aluminum stress condition. The result showed that mean of roots length, shoot length, shoot to root ratio, wet and dry weight of root and shoot exceeded  both parents mean value with moderate to high heritability. All observed characters had continue distribution pattern, and controlled by many genes. Duplicate epistasis additive gene action controlled only root length while other characters were controlled by complementary epistasis gene action.Keywords: gene action, heritability, nutrient culture, selection, tocixity
Seleksi Segregan Gandum (Triticum aestivum L.) pada Dataran Tinggi Wardani, Sri; Wirnas, Desta; Wahyu, Yudiwanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.708 KB) | DOI: 10.24831/jai.v43i1.9590

Abstract

Pedigree selection is a method of selection used in segregating populations which takes the expertise of breeders in achieving the desired goal. This method is implemented on the early generations with a high degree of segregation and is generally performed on highly heritable traits. The purpose of this research was to study the performance of F3 generation segregants and to estimate the genetic parameters to be used for identification and selection of wheat segregants under optimum environmental condition. The research was carried out at the experiment field of  Research Institute for Ornamental Crops, in Cipanas from May to September 2012. Genetic material used were 58 F3 family and six check varieties, namely  Dewata, Selayar, Oasis, Rabe, Basribey, and HP 1744. The experiment used an augmented design. Results of analysis of variance showed that the family effect was significant for days to flowering, days to harvest, percentage of empty florets, number of grains per panicle, grain weight per panicle, number of seeds per plant and seed weight per plant. High heritability estimates were  found in days to flowering, days to harvest, seed filling period, percentage of empty florets, number of grains per panicle, grain weight per panicle, number of seeds per plant, and seed weight per plant. These characters are suitable for yield improvement of tropical highland wheat due to its high heritability values. The mean value of F3 generation changes subsequent to selection either based on grain weight per plant, or based on  number of productive tillers,  number of grain per panicle, and grain weight per plant. Keywords: selection criteria, segregation, tropical, yield
Keragaan dan Keragaman Genetik Karakter Agronomi Galur Mutan Putatif Gandum Generasi M5 Febrianto, Eka Bobby; Wahyu, Yudiwanti; Wirnas, Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (928.019 KB) | DOI: 10.24831/jai.v43i1.9591

Abstract

The cultivation of wheat in Indonesia especially at medium-low altitude faces problems such as high temperatures whicheffect negativelyon the yield. The aims of this research was to obtain informations on performance, genetic variability and heritability of agronomic characters of wheat putative mutant lines on M5 generation. Genotypes evaluated in the study consisted of 98 putative mutant lines of M5 generation and six varieties as checks, namely Dewata, Selayar, Oasis, Rabe, Kasifbey, and Basribey. The research was conducted at Research Station of IOCRI (Indonesian Ornamental Crops Research Institute), Cipanas. Genotypes were arranged in an augmented, randomized block design. Result of the experiment showed that high heritability estimates were observed for plant height (83.03%), tiller number (61.75%), days to flowering (85.41%), days to maturity (67.45%), spike length (70.25%), spikelet number (65.08%), unfilled grain number (71.49%), seed number per spike (75.16%), seed weight per spike (81.69%), and seed weight per plant (74.21%). The estimated genotypic coefficient of variation (GCV) were high for unfilled grain number(26.35%) and seed weight per plant (18.35%). Based on seed weight per plant, 30 best putative mutant lines were selected.Keywords: Augmented design, heritability, Triticum aestivum
Potensi dan Stabilitas Hasil, serta Adaptabilitas Galur-galur Padi Gogo Tipe Baru Hasil Kultur Antera ., Purbokurniawan; Purwoko, Bambang Sapta; Wirnas, Desta; Dewi, Iswari Saraswati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1517.935 KB) | DOI: 10.24831/jai.v42i1.8142

Abstract

ABSTRACTThe objectives of the research were to obtain information on yield potential, adaptability and stability of the upland rice lines. Ten lines and two cultivars were planted at five different locations in November 2010-March 2011. In each location, the experimental design was randomized complete block design with four replications. Observation was done on grains weight per hectar. The results showed that FM1R-1-3-1 achieved the highest productivity (5.65 ton ha-1). Genotype FG1R-36-1-1 was classified as stable genotype by four yield stability analysis as followed: Francis-Kannenberg, Finlay-Wilkinson, Eberhart-Russell and AMMI. Genotypes FG1-70-2-1, FG1R-30-1-5, FG1R-30-1-4 and FG1R-30-1-3 were classified as stable genotypes by three yield stability analysis: Finlay-Wilkinson, Eberhart-Russell and AMMI. AMMI  analysis showed that FG1-6-1-2, FG1-65-1-2, FG1R-30-1-1, FM1R-1-3-1, Fat-4-1-1, Situ Bagendit and Towuti as specific genotypes in certain environment.Keywords: dynamic, new plant type, specific, static, yield
Indikator dan Kriteria Seleksi pada Generasi Awal untuk Perbaikan Hasil Biji Kacang Hijau Berumur Genjah Jambormias, Edizon; Sutjahjo, Surjono Hadi; Mattjik, Ahmad Ansori; Wahyu, Yudiwanti; Wirnas, Desta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2063.11 KB) | DOI: 10.24831/jai.v41i3.8100

Abstract

ABSTRAKPerbaikan hasil kacang hijau dapat dilakukan melalui seleksi langsung maupun seleksi tak langsung berdasarkankriteria seleksi dari satu atau beberapa sifat kuantitatif sebagai indikator seleksi. Penelitian ini bertujuan untuk memilihindikator seleksi bagi hasil biji dan menentukan kriteria seleksi bagi indikator seleksi tersebut pada populasi generasi awalkacang hijau berumur genjah. Penelitian menggunakan rancangan acak tak lengkap berblok bersekat-tersarang 1-tahap.Analisis lintas dan regresi aditif serta genotipik masing-masing digunakan untuk memilih dan menentukan indikator seleksi dan kriteria seleksi. Hasil analisis pengaruh langsung memperlihatkan jumlah biji bernas dan umur berbunga merupakanindikator seleksi bagi hasil biji dengan kriteria seleksi 200 biji pada kisaran umur berbunga 40-46 hari, untuk memperolehhasil > 12 g tanaman. Sebaliknya, hasil analisis pengaruh tak langsung memperlihatkan tinggi tanaman, jumlah cabangdan lama hari panen dapat pula dimasukkan sebagai indikator seleksi. Kriteria seleksi bagi masing-masing indikator seleksiini adalah dua cabang dengan lama hari panen 16-20 hari dan tinggi tanaman sekitar 85 cm untuk menghasilkan 200 biji.Bila dipilih tanaman yang lebih pendek, kriteria seleksi tinggi tanaman 75-80 cm dapat digunakan, namun lama hari panenmenjadi 22-26 hari.-1Kata kunci: analisis regresi, analisis lintas, BLUP, seleksi generasi awal
Penggunaan Mikro Irradiasi Sinar Gamma untuk Meningkatkan Keragaman Genetik pada Varietas Kedelai Argomulyo [Glycine max (L) Merr] Hanafiah, Diana Sofia; Trikoesoemaningtyas, Trikoesoemaningtyas; Yahya, Sudirman; Wirnas, Desta
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.697 KB) | DOI: 10.31258/jnat.14.1.80-85

Abstract

This research used gamma ray irradiation on low doses (micro mutation). The aim of this research was to know the respons of doses level bymicro mutation on gamma ray irridation to the growing and development of Argomulyo variety of Glycine max (L) Merr (M1), the seeds isirradiated by gamma ray by micro mutation was 0 Gy, 50 Gy, 100 Gy, 150 Gy, and 200 Gy. Variation that obtained of each characters atgeneration M1 and M2 influences plants growth and development either through qualitative and quantitative that finally will influence plantsproduction. The average highest genetic variation at M 2 generation of soybean was on 200 Gy doses. Results of the research indicated thatgamma ray irradiation on 200 Gy doses was effective caused of plant variation genetic.
Analisis Genetik dan Seleksi Segregan Transgresif pada Populasi F2 Sorgum Hasil Persilangan B69 × Numbu dan B69 × Kawali Maryono, Marina Yuniawati; ,, Trikoesoemaningtyas; Wirnas, Desta; Human, dan Soeranto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.396 KB) | DOI: 10.24831/jai.v47i2.24991

Abstract

The genetic analysis and selection of transgressive segregants on agronomic characters are required in a sorghumbreeding program for obtaining high proportion homozygosity rapidly in the early generations. This study aimed to obtainthe information on the performance of agronomic characters, estimate genes’ action, broad-sense heritability, coefficient ofgenetic variability, and select transgressive segregant on F2 population of sorghum based on grain yield. The research wasconducted from July 2014 until October 2014 in Dramaga, Bogor. About 300 and 150 individuals F2 populations of B69 ×Numbu and B69 × Kawali, respectively, were used as genetic materials. The results showed that plant height and paniclelength of B69 × Numbu and B69 × Kawali populations were controlled by many genes with additive gene action. The grainyield and grain yield per panicle were controlled by many genes with additive gene action and complementary epistasis,had high heritability, and large coefficients of genetic variability. The transgressive segregant selection had selected 14individuals of B69 × Numbu and 134 individuals of B69 × Kawali populations based on grain yield per panicle.
Co-Authors , Sobir , Trikoesoemaningtyas ,, Trikoesoemaningtyas . SUDARSONO . SUWARSO ACHMAD . Ahmad Ansori Mattjik Aidi Noor Amris Makmur Anggi Nindita Arlianti, Tias Arvita Netti Sihaloho, Arvita Netti Atika Bakti Sari, Atika Bakti Azri Kusuma Dewi, Azri Bambang Sapta Purwoko Buang Abdullah Diana Sofia Hanafiah DIDY SOPANDIE Edi Santosa Edizon Jambormias Eka Bobby Febrianto, Eka Bobby Eni Widajati, Eni Firman Ardiansyah HAJRIAL ASWIDINNOOR Heni Purnamawati Heni Safitri Human, dan Soeranto Imam Widodo Iskandar Lubis Isnaini Isnaini Iswari S. Dewi Iswari Saraswati Dewi Jaisyurahman, Usamah Junaedi, dan Ahmad Karlin Agustina Khairil Anwar Manalu, Victor Manotar Pademan Marlenasari, Lela Marlenasari, Lela Maryono, Marina Yuniawati Mayang Sari Memen Surahman Mizan, Muhammad Rauful Momongan, Jorex Daniel Muhammad Achmad Chozin Muhammad Arif Yudiarto MUNIF GHULAMAHDI Nindita, dan Anggi Nita Kartina, Nita NURHAJATI MATTJIK Nurheni Wijayanto Prasetiyono, dan Joko Purbokurniawan . Putri Andini Mandasari Rini, Erin Puspita Rini, Faradila Median Rini, Faradila Median Rostiana, Otih Sa'diah, Halimah Tus Saragih, Ery Leonardo Saragih, Ratih Irma Khairani Saragih, Ratih Irma Khairani Sayurandi Sayurandi, Sayurandi Sekar Woelan Senjaya, Nofika SESANTI BASUKI Sherly Rahayu Siti Marwiyah Sobir Sobir Sobir, NFN Soeranto Human Sopandie, dan Didy SRI WARDANI Sudarsono, dan SUDIRMAN YAHYA Sumiati, dan Sungkono Sungkono Sunyoto Sunyoto SURJONO HADI SUTJAHJO Suwarno, Punjung Medaraji Tri Budiyanti Trikoesoemaningtyas Trikoesoemaningtyas . Trikoesoemaningtyas Trikoesoemaningtyas TRIKOESOEMANINGTYAS,   Wage Ratna Rohaeni WAGE RATNA ROHAENI Werdhiwati, Pipit Yani Nurhadryani Yudiwanti Wahyu Yulidar Yulidar