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Journal : Jurnal Agripet

PENGGUNAAN PREBIOTIK OLIGOSAKARIDA EKSTRAK TEPUNG BUAH RUMBIA (METROXYLON SAGO ROTTB.) DALAM RANSUM TERHADAP PERFORMAN AYAM PEDAGING Daud, Muhammad; Piliang, Wiranda G; Wiryawan, Komang G; Setiyono, Agus
Jurnal Agripet Vol 9, No 2 (2009): Volume 9, No. 2, Oktober 2009
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.851 KB) | DOI: 10.17969/agripet.v9i2.624

Abstract

Effect of prebiotic oligosaccharide extract rumbia fruit (Metroxylon sago Rottb.) in the ration on broiler performanceABSTRACT. Prebiotic oligosaccharides are thought to provide beneficial effects in the gastrointestinal tract of humans and animals by stimulating growth of selected members of the intestinal microflora. Prebiotic oligosaccharides are defined as nondigestible food ingredients that provide beneficial effects to the host by stimulating the growth of selected microbial members of the gastrointestinal tract. Among the colonic bacteria capable of metabolizing prebiotic oligosaccharides and whose growth is stimulated are species of Lactobacillus and Bifidobacterium. Prebiotic oligosaccharides can be produced in transgli-cosylation reactions catalyzed by glycosidases. Glycosidases from different biological sources have specific ability to catalyze the formation of oligosaccharides with particular chain lengths (usually DP 7) and predominant glycosidic linkages. Oligosaccharide used this research was purified rumbia fruit extract as prebiotic for feed additive in the ration on broiler. The objectives of this research were to study the performance of broiler given of prebiotic oligosaccharide extract rumbia fruit (metroxylon sago Rottb.) in the ration. Two hundred day-old chicks of broiler were divided into three dietary treatments and four replications. Ration used was consisted of: R1 = basal ration (control), R2 = basal ration + 0,4% oligosaccharide extract rumbia fruit, and R3 = basal ration + 0,4% frukto-oligosaccharide (FOS). The variables observed were: feed consumption, body weight, feed conversion ratio, mortality, and production index. The result showed that the performances of the broiler supplemented prebiotic oligosaccharide extract rumbia fruit (R2) was significantly (P 0.05) differences between of feed consumption, body weight, and mortality. It is concluded that of prebiotic oligosaccharide extract rumbia fruit were able to decrease the mortality and feed consumption at six week of age.
DAYA HAMBAT HIDROLISIS KARBOHIDRAT OLEH EKSTRAK DAUN MURBEI Syahrir, S.; Wiryawan, Komang G; Parakkasi, Aminuddin; Winugroho, Winugroho; Ramdania, W.
Jurnal Agripet Vol 9, No 2 (2009): Volume 9, No. 2, Oktober 2009
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.484 KB) | DOI: 10.17969/agripet.v9i2.600

Abstract

Inhibition hydrolysis of carbohydrate by mulberry leaves extractABSTRACT. Mulberry leaves has a great potential as animal feeds because of its high nutrient content, but has deoxynojirimycin (DNJ) active matter. It is potential to inhibit carbohydrate hydrolysis process, come to monosaccharides. The objective of this experiment is to study the inhibiting ability of mulberry leave extract in carbohydrate hydrolysis process. The kinds of carbohydrates were using glucose, maltose, sucrose and starch. This experiment used twenty four of 60 days old male mice (Mus musculus). Diet and water were given ad libitum. Treatment were allocated ina factorial completely randomized design with three replications and two factors containing of completely mulberry leaves extract and variance of carbohydrates. Variable observed were feed consumtion, feed digestibility, body weight gain and blood glucose. The data were analyzed with univariate analysis of variance. The result showed that inclusion of mulberry leaves extract had decrease body weight (P 0,05) and reduce blood glucose (P 0,05).
PENGUJIAN SECARA IN VITRO OLIGOSAKARIDA DARI EKSTRAK TEPUNG BUAH RUMBIA (METROXYLON SAGO ROTTB.) SEBAGAI SUMBER PREBIOTIK Daud, Muhammad; Piliang, Wiranda G; Wiryawan, Komang G; Setiyono, Agus
Jurnal Agripet Vol 9, No 2 (2009): Volume 9, No. 2, Oktober 2009
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.747 KB) | DOI: 10.17969/agripet.v9i2.627

Abstract

In vitro analysis of oligosaccharide from extract rumbia fruit (Metroxylon sago Rottb.) as prebioticABSTRACT. Despite a range of commercially available oligosaccharides there is plenty of room to develop new, functionally enhanced prebiotics. current generation of oligosaccharides was not rationally developed. better understanding of factors determining the prebiotic activity of a particular oligosaccharide. Despite the range of commercially available oligosaccharides mixtures (mainly fructo and galacto-oligosaccharides), very few studies are focused on the mechanisms behind the prebiotic activity of particular oligosaccharides. Probably this lack is due to the unavailability of well characterized oligosaccharide fractions for prebiotic function assessment. The objectives of this research were to asses the ability of lactic acid bacteria in fermentation of oligosaccharide and as prebiotic (in vitro). Material used was oligosaccharide of purified rumbia fruit extract. Analysis of oligosaccharide as prebiotic was conducted in vitro using lactic acid bacteria. The lactic acid bacteria consisted Bifidobacterium bifidum, Bifidobacterium animalis, Lactobacillus bulgaricus and Lactobacillus casei Rhamnosus. The growth media for bacteria was a liquid MRS basic medium where glucose was substituted by oligosaccharide of purified rumbia fruit extract. Incubation was in aerob for Lactobacillus and anaerob for Bifidobacterium in incubator 37oC. The lactic acid bacteria was calculated 24-48 hours during incubation periode. The variables observed were: oligosaccharide component, ability of lactic acid bacteria in fermentation of oligosaccharide, and growth of lactic acid bacteria (Lactobacillus and Bifidobacterium). The result showed that the oligosaccharide component from extract rumbia fruit consisted of: sucrose, stacchiose, and raffinose. The result showed that the oligosaccharide extract rumbia fruit was significantly (P 0.05) the growth of lactic acid bacteria (Lactobacillus and Bifidobacterium) and fermentation of oligosaccharide. It is concluded that oligosaccharide of rumbia fruit extract could be used as prebiotic.