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Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Machrus, Ali; Adrial, Adrial; Armiyanti, Yunita; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.451 KB) | DOI: 10.19184/jid.v15i2.532

Abstract

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite,  the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of  IFN-γ and decreasing level of IL-4 in mice model  of malaria after vaccination with  salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this  mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei.   Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV
Immunogenity of Protein Extract from Salivary Gland of Anopheles aconitus in Malaria Endemic Area Septiawan, Mahful; Budayatin, Budayatin; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.462 KB) | DOI: 10.19184/jid.v18i1.2372

Abstract

Although malaria had ever been virtually eradicated from Indonesia but currently malaria is recognized as a serious re-emerging threat to public health. This disease is caused by malaria parasite which is transmitted to human host by Anopheles mosquitoes as main vector. It has been widely observed that saliva of mosquito that transmits disease contains several factors that could enhance pathogen infection. Therefore, it should be possible to control pathogen transmission by vaccinating the host against the molecule(s) in saliva that potentiate the infection. However, immunogenic specific component in mosquitoes vectors of Malaria has not yet been identified so far. The objective of this study are to analyze protein profile of SDS-PAGE and to know the immunogity the protein extract of salivary gland from potential vector of Malaria i.e. An. aconitus We used immunogenic reaction between salivary gland extract of these vectors against pool of human sera which were collected from endemic area. The reaction conducted by the dot-blot analyze. SDS-PAGE studies showed 15 major polypeptide bands of 284, 100, 84, 75, 66, 57, 53, 48, 45, 38, 33, 29, 15, 14, and 11 kDa. The dot-blot studies showed that the protein extract of salivary gland from An. aconitus are immunogenic.
Relationship Bali Cattle (Bos sondaicus Muller) and Banteng (Bos bibos d'alton) Approach Through The Craniometric Mahdi, Arya; Wiyono, Hidayat Teguh; Suratno, Suratno
Jurnal ILMU DASAR Vol 14 No 2 (2013)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.537 KB) | DOI: 10.19184/jid.v14i2.641

Abstract

Relationship can be seen from the similarity of the cow and bull. One method to find out about the phylogenetic relationship between species is by using cranial morphometry (craniometrics). The purpose of this research was to identify morphological cranium Bali cattle , banteng baluran , and banteng merubetiri based on the parameters measured .This research was conducted in August and ending in October 2013 , held at the National Park Baluran and Merubetiri National Park. The design of this research is descriptive quantitative research , using data analysis kalster ( cluster analysis). Parameters of this research using 15 indicators that have been established in accordance with the research Hayashi , 1982. Materials used in this study are adult male cranium Bali cattle obtained from abattoir ( Slaughterhouse ) Denpasar in January to July 2013 a total of six specimens . Cranium of an adult male bull that used a total of six specimens . Five specimens were collected from the National Park consisting Baluran ( two cranium that is banteng baluran 4 and 5 in 1992 ) and the ( three cranium is banteng baluran1, banteng baluran 2 , and banteng baluran 3 in the 2012-2013 ) . Cranium specimen obtained from the sixth bull collection Merubetiri National Park . Measuring instruments used in this study is the large calipers with a precision level of 0.01 cm . The results of this study showed that the cranium cluster of Bali cattle with coefficient range of 0.016 to 0.042 in contrast to the first cluster is bull cranium specimen glaze 1 bull , bull glaze 2 , 3 baluran bull , and the bull merubetiri 1 , with a coefficient value of 0.047 to 0.161 range . Bali cattle cranium differ significantly with cluster cranium second bull is a bull glaze glaze glaze bull 4 and 5 with the coefficient of 0.162 to 0.251 range. Merubetiri bull glaze and form two clusters , the first cluster bull bull glaze consisting of 1 , 2 bull glaze , glaze bull bull merubetiri 3 and 1 , while the second consists of a cluster bull bull bull glaze glaze 4 and 5 . Turns bull glaze specimens collected in 1992 in contrast to the bull glaze specimens collected in 2012 and 2013. Bull glaze specimens collected in 2012 and 2013, closer to the bull merubetiri specimens . And the bull specimens closer distance coefficient with Bali cattle. The conclusion of this study is the overall specimen Bali cattle entered in a cluster , different from the first cluster and cluster bull bull both . The first cluster by cluster bull bull both are in different clusters. Keywords: cluster analysis , relationship , craniometric , bali cattle and banteng.
Perilaku Bermain Anak Sapi Peranakan Ongole (PO) di Blok Merak, Kawasan Resort Labuhan Merak Taman Nasional Baluran Sohih, Ahmad Mauludin; Wiyono, Hidayat Teguh; Mahriani, M.
BERKALA SAINSTEK Vol 6 No 2 (2018)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v6i2.9302

Abstract

Perilaku bermain merupakan perilaku yang dilakukan hewan dengan tujuan untuk adaptasi, yaitu sebagai latihan dalam menghadapi predator, latihan dalam menyerang, bertarung, seperti melarikan diri atau menyerang lawannya. Perilaku bermain dapat menjadi indikator kesejahteraan hewan (animal welfare). Penelitian ini dilakukan di Blok Merak, kawasan Resort Labuhan Merak Taman Nasional Baluran. Penelitian ini menggunakan lima anak sapi peranakan ongole (PO). Prosedur penelitian ini yaitu; penentuan sampel anak sapi, pelabelan sampel anak sapi, pengamatan perilaku, dan pengukuran kondisi lingkungan. Metode yang digunakan dalam pengamatan perilaku adalah Focal Animal Sampling. Parameter yang diukur yaitu frekuensi dan durasi perilaku bermain anak sapi PO yang meliputi jumping, bucking, running, mock fighting/butting calf, dan mounting. Data hasil pengamatan perilaku bermain anak sapi PO dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa frekuensi dan durasi perilaku bermain yang meliputi jumping dan bucking tiga kali tiga detik, running 27 kali 71 detik, mock fighting/butting calf 45 kali 434 detik, dan mounting yaitu 88 kali 224 detik. Frekuensi dan durasi masing-masing anak sapi PO yaitu anak sapi A 44 kali 215 detik, anak sapi B 24 kali 115 detik, anak sapi C 42 kali 202 detik, anak sapi D 30 kali 83 detik dan anak sapi E 22 kali 120 detik. Kesimpulan penelitian ini adalah ditemukan lima perilaku bermain yang diamati pada anak sapi peranakan ongole (PO) di Blok Merak, kawasan Resort Labuhan Merak yang digembalakan secara liar di savana Taman Nasional Baluran. Kata Kunci: Perilaku Bermain, Anak sapi Peranakan Ongole (PO), dan Taman Nasional Baluran.
PRODUKSI GUM ARABIC BALURAN SEBAGAI PENDUKUNG PENGEMBANGAN WISATA KAMPUNG BANTENG DI KARANG TEKOK SEBAGAI WILAYAH PENYANGGA TN BALURAN Kusumah, Maulana S.; Wiyono, Hidayat Teguh; Subekti, Agus; Muzakhar, Kahar; Winarsa, Rudju
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 4 No 1 (2020): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Forum Dosen Indonesia JATIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v4i1.272

Abstract

This article is the result of PPDM (Mitra Desa Service Program) about Baluran bos javanicus (Banteng) village tourism. Development of bull village tourism is an effort to solve the problem of wild grazing in Bunaken National Park. In the event of Banteng Village Tourism, it is necessary to support tourism, namely creative industries, agro-tourism, and NTFP production (non-timber forest products). One of the NTFPs that is relied upon is Arabic gum. Currently, cattle breeders in the banteng village area have been able to produce Arabic gum as a result of the introduction of tapping technology by the 2019 PPDM team. The dedication method is in the form of dissemination and field practice. Three groups representing breeders were trained to tap acacia gum through a drilling method combined with ethephon induction as GIS. One week after application, the group begins harvesting gum and submits the results to the group leader. Then the group leader sends the results to the Cooperative in Pondok Pesantren Assalam, Sumberanyar, Banyuputih Situbondo. The amount of Baluran Arabic gum that was collected by the group for three months reached 143.9 kg. This service activity concludes that the strength in producing Baluran Arabic gum is significant in improving the welfare of breeders in supporting the maintenance and retention of a Banteng.