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ANTIBACTERIAL ACTIVITY OF WATER LILY SEED EXTRACT TOWARD DIARRHEA-CAUSING PATHOGENIC BACTERIA Fitrial, Yuspihana; Astawan, Made; Soekarto, Soewarno S; Wiryawan, Komang G; Wresdiyati, Tutik; Khairina, Rita
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objectives of this study was to observe antibacterial activities of water lily seed, against diarrhea-causing pathogenic bacteria as well as lactic acid bacteria; phytochemistry components in water lily seed and to evaluate each component?s activities against pathogen bacteria. Extraction of antibacterial components in the seed was done by fractional extraction methods using solvent based on its polar level, i.e. hexane, ethyl acetate and ethanol. The activities of each extract was tested by using diarrhea-causing bacteria, Enteropathogenic Escherichia coli K.1.1 (EPEC K1.1) and S. typhimurium with agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated with plate counting. Qualitative phytochemical tests were performed on all extracts. Fractionation was performed on extract with the largest antimicrobial activity by using thin-layer chromatography. The activities of each fraction were tested qualitatively by bio-autography method on thin layer chromatographic plates. The water lily seed had an antibacterial activity against EPEC K.1.1 and Salmonella typhimurium, especially in ethyl acetate extract. Ethanol extract had the same, yet lower activity. Ethyl acetate and ethanol extract of the seed did not show inhibition against the growth of lactic acid bacteria (Lactobacillus sp) and Bifidobacterium bifidum. The MIC and MBC values of the ethyl acetate extract on EPEC K1.1 were 0.89 (mg/mL) and 1.33 (mg/mL), respectively, while similar values of that on S. Typhimurium were 1.11 (mg/mL) and 1.33 (mg/mL¬), respectively. Phytochemistry components within ethyl acetate extract were alkaloids, flavonoids, tannins, glycosides, saponins, and triterpenoids. All fractions in the ethyl acetate extract had antimicrobial activities against EPEC K.1.1 and S. Typhimurium. These fractions were thought to inhibit the growth of the test-microbes by synergic action of each component.Key words: water lily seed, antibacterial, ethyl acetate extract
TERIPANG PASIR MENINGKATKAN KANDUNGAN ANTIOKSIDAN SUPEROKSIDA DISMUTASE PADA PANKREAS TIKUS DIABETES (SEA CUCUMBER INCREASED ANTIOXIDANT SUPEROXIDE DISMUTASE IN THE PANCREATIC TISSUE OF DIABETIC RATS) Wresdiyati, Tutik; Karmila, Ani; Astawan, Made; Karnila, Rahman
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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High level of blood glucose is an indicator for diabetes mellitus (DM) condition. The condition iscaused by low level of insulin secretion or impairement of insulin receptor. The number of DM patientincreases every year. The World Health Organization reported that the number of DM patient in Indonesiawas the 4th highest in the world, after following China, India, and the United States of America, respectively.This study was conducted to analyze the effect of sea cucumber (Holothuria scabra J) on the profile ofantioxidant copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the pancreatic tissues of diabetic rats. Atotal of 25 male white rats (Sprague Dawley) were used in this study. They were divided into five groups;(1) negative control (KN), (2) positive control, diabetic rats (KP), (3) diabetic rats treated with hydrolyzatedprotein of sea cucumber (HDL), (4) diabetic rats treated with concentrated protein of sea cucumber (KST),and (5) diabetic rats treated with isolated protein of sea cucumber (ISL), respectively. Diabetic conditionwas obtained by alloxan injection 110 mg/kg bw. The treatments were done for 28 days. At the end oftreatment period, the rats were sacrificed and pancreatic tissues were collected and fixed in Bouin solution and then processed to paraffin embedding standard method. The tissues were then stained withimmunohistochemical staining techniques using monoclonal antibody of Cu, Zn-SOD. The results showedthat treatment of HDL, KST, and ISL of sea cucumber (Holothuria scabra J) increased the content ofantioxidant Cu, Zn-SOD either in Langerhans islets and acinar cells of pancreatic tissues-diabetic rats.The HDL of sea cucumber treatment gave the best effect in increasing the antioxidant content of Cu, Zn-SOD in pancreatic tissue of diabetic rats.
COMPARATIVE MICROANATOMY OF THE LOCAL GOAT AND SHEEP PANCREAS ISLETS WITH A SPECIAL REFERENCE TO THE DISTRIBUTION AND RELATIVE FREQUENCY OF GLUCAGON PRODUCING CELLS Adnyane, I Ketut Mudite; Novelina, Savitri; Sari, Dwi Kesuma; Wresdiyati, Tutik; Agungpriyono, Srihadi
Media Veteriner Vol. 8 No. 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.
MORPHOLOGY AND HISTOMORPHOMETRY OF TESTIS AND EPIDIDYMIS OF KACANG GOAT (CAPRA SP.) AND LOCAL SHEEP (OVIS SP.) Noviana, Citra; Boediono, Arief; Wresdiyati, Tutik
Media Veteriner Vol. 7 No. 2 (2000): Media Veteriner
Publisher : Media Veteriner

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Penelitian ini bertujuan untuk mempelajari morfologi dan histomorfometri testis dan epididymis kambing kacang (Capra sp.) dan domba lokal (Ovis sp.) dengan umur 1-1,setahun. Secara makroskopik, keliling, berat dan volume testis kambing kacang lebih kecil (P
EVALUASI KESEPADANAN MUTU GIZI TEMPE KEDELAI PANGAN REKAYASA GENETIK (PRG) DAN NON-PRG SERTA DAMPAK KONSUMSINYA PADA TIKUS PERCOBAAN Maskar, Dadi Hidayat; Hardinsyah, Hardinsyah; Damayanthi, Evy; Astawan, Made; Wresdiyati, Tutik
Jurnal Gizi dan Pangan Vol. 10 No. 3 (2015)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.768 KB) | DOI: 10.25182/jgp.2015.10.3.%p

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ABSTRACTThis study was conducted to evaluate the effect of tempe that were made from Genetically Modified (GM) and non-GM soybean on protein quality, malondialdehide (MDA) levels, intracellular antioxidant superoxide dismutase (SOD) activity in the liver and kidneys, as well as spermatozoa profile of experimental rats. Fourty five Sprague Dawley rats divided into eight treatment grups and one control, fed with tempe and soybean from GM and non-GM at 10% and 20% concentrations for 90 days. The results showed that there was no significant difference in term of protein quality, tempe made from GM soybean is substantially equivalent with tempe made from non-GM soybean. Results showed that group which was given ration of 10% protein from conventional soybean had lower liver and kidney MDA levels as compared to GM tempe 10% and 20% groups, but was not significant compared to conventional soybean 20% and casein 10% groups. While the value of liver and kidney SOD activity were not significantly different (p>0.05) between the groups of rats. There was no significant differences among the spermatozoa profiles treatment groups and control and they were within normal condition. Results of protein quality, MDA, SOD, and spermatozoa profile showed that tempe made from GM soybean was substantially equivalent with the non-GM soybean.Keywords: GM soybean, MDA, non-GM tempe, SOD, spermatozoa profileABSTRAKTujuan penelitian ini adalah untuk mengevaluasi pengaruh tempe Pangan Rekayasa Genetik (PRG) dan non-PRG meliputi kualitas protein, kadar malondialdehida (MDA) hati dan ginjal, aktivitas superoksida dismutase (SOD) hati dan ginjal, dan profil spermatozoa pada tikus percobaan. Sebanyak 45 tikus terbagi ke dalam delapan perlakuan dan satu kontrol yang diberikan perlakuan dengan ransum tempe dan kedelai, baik PRG maupun non-PRG, dengan konsentrasi 10% dan 20% selama 90 hari. Hasil percobaan menunjukkan bahwa tidak ada perbedaan yang signifikan dalam kualitas protein, tempe PRG memiliki nilai yang sama dengan tempe non-PRG. Tikus yang diberi ransum 10% protein kedelai non-PRG mempunyai nilai MDA hati dan ginjal yang lebih rendah dibandingkan dengan tempe PRG 10% dan 20%, tetapi tidak signifikan dengan kelompok kedelai non-PRG 20% dan kasein 10%. Sementara itu, nilai SOD hati dan ginjal tidak signifikan antar grup perlakuan (p>0,05). Tidak ada perbedaan yang signifikan pada profil spermatozoa antar perlakuan. Hasil dari kualitas protein, MDA, SOD, dan profil spermatozoa menunjukkan bahwa kedelai dan tempe PRG memiliki kesamaan substansial dengan kedelai dan tempe non-PRG.Kata kunci: MDA, profil spermatozoa, SOD, tempe kedelai PRG, tempe kedelai non-PRG
PENGARUH KONSUMSI TEMPE KEDELAI GROBOGAN TERHADAP PROFIL SERUM, HEMATOLOGI DAN ANTIOKSIDAN TIKUS Astawan, Made; Wresdiyati, Tutik; Sirait, Jefriaman
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 2 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.423 KB) | DOI: 10.6066/jtip.2015.26.2.155

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Tempe is a potential source of protein for 250 million people in Indonesia. Indonesian soybean demand reached 2.2 million tons per year, and 1.3 million tons of which are used for tempe production. Approximately 70% of the soybeans are imported from different countries. To improve national food security, it is important to use local soybean as a raw material. Therefore, this study used Grobogan local soybean as raw material. The purpose of this study was to evaluate the impact of the consumption of tempe (from local soybean Grobogan) in the long term, ie over a 90-day subchronic test using rats as a model. Parameters measured were serum biochemical profile, hematology, malonaldehyde (MDA), and superoxide dismutase (SOD) in liver and kidney organs of rats. This research was conducted using three groups of rats were fed tempe, boiled soybean, and casein as a source of protein in the ration. Data feed convertion efficiency showed tempe of Grobogan soybean had a better quality protein than that of boiled soybean and casein. As compared to the groups of rats fed boiled soybean and casein, the tempe group had higher HDL cholesterol, but not significantly different in hematological parameters, MDA and SOD in liver and kidney organs. This suggests consumption of tempe made from Grobogan soybean in the long term does not have a negative impact on health.
YOGHURT SINBIOTIK BERBASIS PROBIOTIK LOKAL DAPAT MENCEGAH DIARE DAN MENGUBAH STATUS HEMATOLOGI TIKUS (SYNBIOTIC YOGHURT BASED ON INDIGENOUS PROBIOTIC: IT’S EFFECT ON DIARRHEA AND HEMATOLOGICAL STATUS IN RATS) Astawan, Made; Wresdiyati, Tutik; ., Suliantari; Nababan, Yenni MS
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objective of this study was to observe the effect of functional synbiotics yoghurt (made ofindigenous probiotic + oligofructosaccharides) in rats that were infected with Enteropathogenic Escherichiacoli (EPEC), and to detect its effect on hematological status. A total of 25 male Sprague Dawley rats wereused in this study and divided into five treatment groups: (i) negative-control; (ii) positive-control; (iii)synbiotic yoghurt; (iv) synbiotic yoghurt + EPEC; and (v) conventional prebiotic yoghurt. Yoghurt (109 cfu/mL lactic acid bacteria) was given orally using feeding tube at day one until day 4. At day 8 ? day 14animals were infected with EPEC (107 cfu/mL) orally using feeding tube; at day 22 blood samples werecollected for hematology analysis (erythrocytes, hematocrit (Hct), hemoglobulin (Hb), platelets, andleucocytes). Post infection with the EPEC diarrhea was developed in rats both in the control positivegroups and in the synbiotic yoghurt + EPEC group. Animals in the positive-control group had a significantlyhigher thrombocytes and leucocytes counts and Hct compared to that in animals in the other groups(P<0.01). Whereas there were no significant effect on the erythrocytes counts and Hb (P>0.05). The treatmentwith synbiotic yoghurt, in both synbiotic yoghurt only and synbiotic yoghurt + EPEC groups significantlyreduced the platelets and leucocytes counts and Hct of rats
EKSTRAK BATANG SIPATAH-PATAH MENINGKATKAN PROLIFERASI DAN DIFERENSIASI SEL PUNCA MESENKIMAL SUMSUM TULANG (CISSUS QUADRANGULA SALISB STEM EXTRACT INCREASED PROLIFERATION AND DIFFERENTIATION OF RATS BONE MARROWMESENCHYMAL STEM CELL) Ceriana, Ria; Djuwita, Ita; Wresdiyati, Tutik
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Acehnese people uses Cissus quadrangula (CQ) Salisb stem traditionally for treatment of variousbone disease. can differentiate into many different cell types such as osteoblast, adipocyteand chondrocytes.A study was conducted to determine the potential uses and the optimal dosage of C. quadrangula(CQ)extractin increasing the proliferation and differentiation of Mesenchymal stem cells derived from bone marrowof rat osteocytes. Mesenchymal stem cells were isolated from femoral and tibial rat bone marrow. Thecells were cultured according to the experimental group consisting of five the treatment groups each ofwhich has 4 replication. The cells were cultured in modified Dulbecco?s modified eagles?s medium (mDMEM).Control were cultured in medium without Cissus quadrangula Salisb stem extract whereas the treatmentgroup were cultured in medium with k 0,1 mg/mL, 0,3 mg/mL, 0,6 mg/mL, dan 0,9 mg/mLCissus quadrangulaSalisb stem extract. The level of the cell proliferation was determined by populationdoubling time (PDT) method. Cell differentiation was determined by counting cells and determining the diameter of osteoblastdan osteocytes.The result showed that CQ stem extract reduced PDT valuesignificantly (P<0,01) ascompared to those of control group. This showed that CQ stem extract increased the rat bone marrow stemcells. The number of h osteoblast in control group were significantly lower than those in CQ stem extracttreatment groups. The highest osteocyte population was observed in 0,3 mg/mL CQ extract treatmentgroup. The CQ stem extract can increase differentiation and proliferation of rat bone marrow masenchymalstem cells into osteoblastand osteocytes with the optimal dose of 0,3 mg/mL.
THE PROFILE OF SUPEROXIDA DISMUTASE AND MALONDIALDEYDE LEVEL IN THE LIVER TISSUE OF HYPERCHOLESTEROLEMIC RATS TREATED WITH HOLOTHURIA NOBILIS POLYSACCHARIDE Ulhusna, Fitrah Asma; Winarto, Adi; Wresdiyati, Tutik
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i2.13189

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The aim of this research was to analyze the profile of superoxide dismutase (SOD) and malondialdehyde (MDA) on the liver tissue of hypercholesterolemic rats which were given Holothuria nobilis polysaccharides (HNP). A total of 15 male rats strain Sprague Dawley were divided into prevention and curative groups. Prevention group consisted of negative/non-hypercholesterolemic group (K-), positive/hypercholesterolemic group (K+), and hypercholesterolemic prevention group which were given 1% cholesterol diet and HNP at dose of 400 mg/kg bw (PCh). The treatments were given for 28 days. The curative group was consisted of the hypercholesterolemic group, which was given 1% cholesterol diet for 28 days, then followed by standard diet for 28 days (Ch), and the hypercholesterolemia curative group which was given 1% cholesterol diet for 28 days, then followed by 400 mg/kg bw HNP for 28 days (ChP). The antioxidant activity of HNP was analyzed by DPPH method. At the end of study the liver tissue was collected and analyzed for MDA, SOD while Cu,Zn-SOD was analyzed by immunohistochemical technique. The results showed that the antioxidant activity of HNP was weak. The MDA level (µg/g) in K-, K+, PCh, Ch, and ChP groups were 1.19±0.6; 3.37±0.79; 0.29±0.14; 9.11±0.72; and 3.14±1.06, respectively. The SOD activities (U/g) in K-, K+, PCh, Ch, and ChP groups were 2141.11±83.88; 1541±211.69; 2096.67±166.66; 1063.33±88.19; 1685.55±167.77, respectively. The immuno reactivity of Cu,Zn-SOD showed that HNP could increase Cu,Zn-SOD in the liver tissues of both groups. This study concluded that the HNP increased SOD activity, Cu,Zn-SOD antioxidant content, and decreased MDA levels in the liver tissues of hypercholesterolemic rats in both preventive and curative groups.
PROFIL IMUNOHISTOKIMIA ANTIOKSIDAN COPPER, ZINC-SUPEROXIDE DISMUTASE (CU, ZN, -SOD) PADA GINJAL TIKUS PERINATAL DAN NEONATAL Wresdiyati, Tutik; Adnyane, I Ketut Mudite; Prabandari, Silvia Arin; Sofiawati, Sofiawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.2913

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The aim of the research was to observe the profile of intracellular antioxidant, especially copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the kidney of perinatal and postnatal rats immunohistochemically. A total of twuelve Wistar rats were used for this research and they were divided into four groups that are 18 days (Fe18) and 20 days old fetus (Fe20), one day (N1) and three days neonatus (N3). There are three rats in each groups. Kidney from each group were taken and processed for routin paraffin embedding methods. The tissues sections were observed with Hematoxylin-eosin (HE) and immunohistochemical technique for Cu, Zn-SOD. Cu,Zn-SOD in the kidney were observed qualitatively in medulla, glomerulus dan cytoplasm of tubuli renalis proximalis and tubuli renalis distails, as well as quantitatively in the nucleus of tubuli renalis cells. The results showed that Cu, Zn-SOD increased in the kidney of perinatal rats, 20 days old fetus (Fe20), then it decreased in the tissues of the one days neonatus (N1), then were increased again in the kidney of three days neonatus (N3) group rats.