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STATUS HEMATOLOGI DAN RESPON IMUN IKAN KOI (CYPRINUS CARPIO) YANG TERINFEKSI MYXOBOLUS SP. DENGAN TREATMENT DIMILIN (HEMATOLOGICAL STATUS AND IMMUNE RESPONSE OF KOI FISH (CYPRINUS CARPIO) INFECTED BY MYXOBOLUS SP. WITH TREATMENT OF DIMILIN) Setiawan, feri; Yanuhar, Uun; Kurniawan, Andi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 1 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.461 KB) | DOI: 10.14710/ijfst.15.1.80-85

Abstract

Koi merupakan ikan hias favorit di pasar nasional dan internasional. Proses budidaya untuk menghasilkan koi dengan kualitas terbaik masih menjadi kendala, salah satunya adanya infeksi parasit Myxobolus sp. yang dapat menyebabkan kematian massal dalam waktu singkat. Myxobolus yang menyerang jaringan ikan dapat menyebabkan kerusakan prah pada jaringan. Banyak cara dilakukan oleh pembudidaya Ikan Koi untuk menghindari infeksi Myxobolus, yaitu dengan pengobatan secara alami dan menggunakan bahan kimia. Penelitian ini bertujuan untuk mengetahui efek hematologi dan respon imun pada Ikan Koi yang terinfeksi parasit Myxobolus dengan pengobatan dimilin. Metode yang digunakan adalah metode eksperimental dengan perlakuan kontrol ikan sehat (A), Ikan koi yang terinfeksi oleh Myxobolus sp. tanpa perlakuan dimilin (B), ikan koi terinfeksi Myxobolus sp dengan perlakuan dimilin 0,02 mg (C), dan ikan yang terinfeksi Myxobolus sp dengan perlakuan dimilin 0,01 mg (D). Pengambilan darah ikan dilakukan dengan metode punksi pembuluh darah bagian caudal dan dilakukan pengamatan hematologi, serta dilakukan pengamatan imunohistokima dengan melihat persentase Diaminobenzidine (DAB) pada ikan. Hasil rata-rata pada perhitungan hematologi leukosit dan eritrosit tertinggi  ditemukan pada ikan yang terinfeksi Myxobolus sp tanpa perlakuan dimilin (B), dengan eritrosit 2.913.334 sel / mm3 dan leukosit 197.184 sel / mm3. Hasil respon imun berdasarkan pengamatan imunohistokimia didapatkan nilai DAB perlakuan A adalah 16%, nilai DAB perlakuan B 42,7%, nilai DAB perlakuan C 25,6%, dan nilai DAB perlakuan D 31,4%. Kesimpulan penelitian ini adalah pemberian dimilin dapat mempengaruhi respon hematologi dan dapat mempengaruhi respon imun pada ikan yang dapat ditunjukkan dengan Interleukin-6 pada ikan dengan hasil DAB immunoratio. Koi is a favorite ornamental fish in the national and international markets. Cultivation process to produce koi with the best quality is still have problem, one of the problem is parasitic infection of Myxobolus sp. which can cause mass death in a short time. Myxobolus attacks fish tissue can cause severe damage to tissue. Many ways were used by koi fish farmers to avoid Myxobolus infection which  with natural treatment and using chemicals drug. This study aims to determine the hematological effects and immune respons in the koi fish infected with the Myxobolus and treated with dimilin. Experimental method was used in this research with control treatment of healthy fish (A), koi fish infected by Myxobolus sp. without treatment (B), koi fish infected by Myxobolus sp with dimilin treatment 0.02 mg (C), and koi fish infected by Myxobolus sp with dimilin treatment 0.01 mg (D). fish Blood samples were collected with puncture of caudal vessel method and then haematological observations to blood samples, and immunohistokima observations by looking percentage of Diaminobenzidine (DAB) in fish. Highest hematological Results of leukocytes and erythrocytes were found in treatment B, with erythrocytes result 2,913,334 cells / mm3 and leukocyte 197,184 cells / mm3. Immune response result based on immunohistochemical observations with DAB value of treatment A is 16%, treatment B is 42.7%, treatment C is 25.6%, and treatment D is 31.4%. Conclusion in this study that dimilin treatment can affect hematological response and immune response in fish which can be shown with Interleukin-6 in fish with DAB immunoratio results. 
UJI IN VITRO DAN KARAKTER PROTEIN EKSTRASELULER (ECP) EDWARDSIELLA TARDA DENGAN KONSENTRASI ETHANOL BERBEDA (IN VITRO TEST AND CHARACTER OF EXTRACELLULAR PROTEIN (ECP) EDWARDSIELLA TARDA WITH DIFFERENT ETHANOL CONCENTRATION) Herlina, Cucun; Yanuhar, Uun; Maftuch, Maftuch
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 2 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.923 KB) | DOI: 10.14710/ijfst.15.2.105-111

Abstract

Protein ekstraseluler (ECP) bakteri memiliki kemampuan imunogenik dan dapat meningkatkan sistem imun tubuh inang. Komponen ECP diantaranya flagellin, protease dan metalloprotease mampu berdifusi pada sel inang untuk mengaktifkan respon imun. Tujuan penelitian adalah untuk mengetahui produksi protein ekstraseluler E. tarda dengan presipitasi ethanol pada konsentrasi berbeda sebagai potensi protein imunogenik secara in vitro. Metode penelitian yaitu kultur bakteri E. tarda, preparasi protein ekstraseluler, presipitasi protein, konsentrasi protein ditentukan denganspektrofotometer dan SDS-PAGE, uji in vitro protein ECP E. tarda, dan uji nilai RPS. Hasil protein ekstraseluler yang diperoleh dengan presipitasi ethanol tertinggi pada konsentrasi 90% sebanyak 5,69 mg/ml dan terendah pada konsentrasi 80% sebanyak 4,81 mg/ml. Hasil karakterisasi protein dengan SDS-SDSPAGE antara 30-60 kDa. Hasil uji in vitro vaksin E. tarda (presipitasi ethanol 100%) baik dari uji viabilitas maupun sterilitas menunjukkan tidak terdapat koloni yang tumbuh. Hasil nilai RPS pada ikan nila (Oreochromis niloticus) mencapai 100% dengan masa pemelihaaraan 9 hari pasca imunisasi. ECP bacteria have immunogenic abilities and can increase the host's immune system. ECP components which are flagellin, protease, metalloprotease can diffuse to host cell for the activation immune response. The study aimed to determine the production of extracellular protein E. tarda with ethanol precipitation at different concentrations as a potential immunogenic protein by in vitro test. The research methods were E. tarda bacterial culture, extracellular protein preparation, protein precipitation, protein concentration determined by spectrophotometer and SDS-PAGE, in vitro ECP E. tarda protein test, and RPS value test. The results of extracellular protein obtained by the highest ethanol precipitation at a concentration of 90% as much as 5.69 mg/ml and the lowest at 80% concentration as much as 4.81 mg/ml. The results of the characterization of proteins with SDS-SDS-PAGE between 30-60 kDa. The results of the in vitro test of E. tarda vaccine (100% ethanol precipitation) from both the viability and sterility tests showed that there were no growing colonies. The results of the RPS in tilapia (Oreochromis niloticus) reach 100% with a maintenance period of 9 days after immunization.
In-Vivo Test of Spirulina sp as Inducer of β-Actin In Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis Irawanto, Yovan Endik; Yanuhar, Uun; Kurniawan, Andi
JFMR-Journal of Fisheries and Marine Research Vol 2, No 3 (2018): JFMR VOL 2 NO 3
Publisher : JFMR-Journal of Fisheries and Marine Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.566 KB) | DOI: 10.21776/ub.jfmr.2018.002.03.11

Abstract

Cantang grouper fish (Epinephelus fuscoguttatus-lanceolatus) is a result of hybridized fish between a female of tiger grouper fish (Epinephelus fuscoguttatus) and male of kertang grouper (Ephinephelus lanceolatus). In the development of cultivation, there are many problems, one of them infected with the class of virus Nodaviridae, namely Viral Nervous Necrosis (VNN). Fish had a defense against cellular immunity against the VNN virus by β-actin. The aims of this research to explore the crude extract of Spirulina sp as a β-actin inducer for the anti-inflammatory immune system in grouper fish against VNN attack. The method used in this research is experiment methods.  Crude extracts of Spirulina sp (33 μg/ml) were conducted by feeding orally to groupers, and VNN infections were conducted by feeding the already positive VNN meat. Detection of VNN using RT-PCR, however β-actin detection using PCR, and IHC in the organ of Cantang grouper fish. The results showed that the percentage of DAB value of control fish (14.0%), fish treated with Spirulina sp (25.7%), fish treated with VNN (31.9%), and fish treated with Spirulina sp extract and VNN infection (32.4%). The percentage of DAB values were indicated by the detection of the target gene β-actin. Immunity in fish increases with the addition of Spirulina sp. The increased β-actin expression may also be used as an indicator of a grouper's body defense against VNN infection.
THE CHARACTERIZATION OF HAEMAGLUTININ PROTEIN OF VILI VIBRIO ALGINOLYTICUS AS ADHESION MOLECULE ON EPITHELIAL CELLS RECEPTOR OF HUMPBACK GROUPER (CROMILEPTES ALTIVELIS) Yanuhar, Uun
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.3499

Abstract

Vibriosis was a crucial problem and a primer infection causing mortality of humpback grouper.Virulence factor of vibrio, such as vili, is an adhesion molecule protein based on haemaglutination test toblood serum of humpback grouper. This research was aimed to characterize an adhesion molecule of viliprotein of V. alginolyticus on epithelial cells of Humpback grouper as haemaglutinin protein. Themethods are laboratory exploration by isolate epithelial cells of Humpback grouper, characterize a receptorand test vili protein to adhesion molecule that is epithelial cell of Humpback grouper. The result of proteinhaemaglutinin test to vili V. alginolyticus show that an adhesion molecule with a molecule weight of38.98 kDa is an adhesin vili V. alginolyticus and a haemaglutinin. The adhesion pattern of V.alginolyticus on epithelial cells of Humpback grouper exposed to protein haemaglutinin of vili show bothdiffuse and localize pattern. Research conclusion was the haemaglutinin protein of vili V. alginolyticuswas an adhesin having a role to both virulence process of bacteria and attachment process to receptor ofepithelial cells of Humpback grouper.Keywords: adhesion molecule, epithelial cell, vili, V. alginolyticus, C. altivelis
EFEK PEMBERIAN PROBIOTIK TERHADAP IKAN KOI (CYPRINUS CARPIO) YANG TERINFEKSI MYXOBOLUS SP. (EFFECT OF PROBIOTICS TREATMENT ON KOI CARP (CYPRINUS CARPIO) INFECTED WITH MYXOBOLUS SP.) Caesar, Nico Rahman; Yanuhar, Uun; Musa, Muhammad
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 1 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.501 KB) | DOI: 10.14710/ijfst.15.1.60-65

Abstract

Myxobolus merupakan ektoparasit yang berbahaya dan dapat mengakibatkan kematian hingga 80%. Dalam praktek akuakultur, probiotik telah digunakan dalam beberapa tahun terakhir probiotik menjadi bagian integral dari praktik budaya untuk meningkatkan pertumbuhan dan ketahanan terhadap penyakit. Tujuan penelitian ini mengetahui pengaruh pemberian probiotik terhadap hematologi dan respon imun ikan koi yang terserang Myxobolus sp. yakni eritrosit dan leukosit serta ekspresi Nuclear Factor-kappa Beta  (NF-kB). Metode yang digunakan yaitu metode eksperimen. Dalam penelitian ini dibagi ke dalam 4 perlakuan yaitu, perlakuan (A) Kontrol, (B) ikan koi terinfeksi Myxobolus sp., (C) ikan koi terinfeksi Myxobolus sp. dengan pemberian probiotik dosis 0,55 ml dan (D) ikan koi terinfeksi Myxobolus sp. dengan pemberian probiotik dosis 1,1 ml. Metode pemberian perlakuan dengan menambahkan probiotik pada 30 liter air pada bak pemeliharaan. Kemudian dilakukan pengamatan eritrosit dan leukosit. Serta dilakukan Imunohistokimia menggunakan antibodi NF-kB pada jaringan insang. Berdasarkan hasil pengamatan eritrosit diperoleh nilai rata ? rata pada perlakuan A sebesar 1.666.667 sel/mm3, perlakuan B sebesar 1.940.000 sel/mm3, perlakuan C sebesar 1.776.667 sel/mm3 dan perlakuan D sebesar 1.836.667 sel/mm3. Hasil pengamatan leukosit diperoleh nilai rata ? rata pada perlakuan A sebesar 119.800 sel/mm3, perlakuan B sebesar 492.800 sel/mm3, perlakuan C sebesar 308.533 sel/mm3 dan perlakuan D sebesar 318.400 sel/mm3. Selanjutnya, berdasarkan hasil Imunohistokimia  didapatkan hasil pada perlakuan A nilai DAB sebesar 15,1%, perlakuan B nilai DAB sebesar 31,7%, perlakuan C nilai DAB sebesar 53,5% dan pada perlakuan D nilai DAB sebesar 47,5 %. Dari penelitian ini dapat disimpulkan bahwa pemberian probiotik berpengaruh terhadap eritrosit dan leukosit serta dapat meningkatkan ekspresi NF-KB sebagai respon imun pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Dosis optimal pemberian probiotik yaitu pada dosis 0,55 ml. Myxobolus is a dangerous parasite that can kill up to 80% on koi carp pond.. Probiotic intake has been proven to change the composition of the microbiota, and therefore helps in the recovery of microbiota that are disrupted (by antibiotics or other risk factors) into favorable or normal compositions. The purpose of this study was to determine the effect of probiotic administration on hematology and the immune response of koi fish attacked by Myxobolus sp. namely erythrocytes and leukocytes and expression of Nuclear Factor-kappa Beta (NF-kB). The method used was an experimental by providing probiotics to fish that promoted Myxobolus sp. In this study it was divided into 4 treatments namely, (A) Control, (B) koi carp infected by Myxobolus sp., (C) koi carp infected by Myxobolus sp. with probiotic doses of 0.55 ml and (D) koi fish infected by Myxobolus sp. by administering a probiotic dose of 1.1 ml. The treatment methods were the fish immersed into 30 L of water that added by Probiotic. The hematological observation of koi fish,  erythrocytes and leukocytes, was observed and immunohistochemistry using NF-KB antibodies in the gill tissue which is the target of the entry of Myxobolus sp. on Koi fish. Based on the results of the treatment of erythrocytes obtained the value of A handling is 1,666,667 cells / mm3, handling B is 1,940,000 cells / mm3, handling C is 1,776,667 cells / mm3 and handling D is 1,836,667 cells / mm3. The results of leukocyte observations obtained an average value on treatment A of 119,800 cells / mm3, treatment B was 492,800 cells / mm3, treatment C was 308,533 cells / mm3 and treatment D was 318,400 cells / mm3. Furthermore, based on the results of Immunohistochemistry, the results of handling A DAB value of 15.1%, handling B DAB value of 31.7%, handling C DAB value of 53.5% and at the time of treatment D DAB value of 47.5%. From this study it can be concluded that the treatment of probiotics affects erythrocytes and leukocytes and can increase the expression of NF-KB as an immune response in koi fish (Cyprinus carpio) infected with Myxobolus sp. The optimal dose of probiotics is at a dose of 0.55 ml 
EFEK PEMBERIAN DELTAMETHRIN TERHADAP EKSPRESI CD4 PADA IKAN KOI (CYPRINUS CARPIO) YANG TERINFEKSI MYXOBOLUS SP. (EFFECT OF DELTAMETHRIN ON CD4 EXPRESSION IN KOI FISH (CYPRINUS CARPIO) INFECTED BY MYXOBOLUS SP.) Sumsanto, Muhammad; Yanuhar, Uun; Hertika, Asus Maizar Suryanto
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 2 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (692.787 KB) | DOI: 10.14710/ijfst.15.2.94-98

Abstract

Parasit yang sering menyerang ikan koi adalah Myxobolus sp. Ikan yang terinfeksi Myxobolus sp. akan mengalami gejala klinis berupa nodul kemerahan dan pembengkakan pada organ insang. Penelitian bertujuan untuk mengetahui pengaruh pemberian deltamethrin terhadap ekspresi CD4 pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Pada penelitian ini dibagi menjadi 4 perlakuan (A) Kontrol, perlakuan (B) ikan koi terinfeksi Myxobolus sp., perlakuan (C) ikan koi terinfeksi Myxobolus sp. dengan pemberian deltamethrin dosis 0,5 µl/g dan perlakuan (D) ikan koi terinfeksi Myxobolus sp. dengan pemberian deltamethrin dosis 1 µl/g. Kemudian dilakukan pengamatan eritrosit, leukosit dan immunohistokimia untuk mengetahui ekspresi CD4 pada usus. Berdasarkan hasil penelitian didapatkan eritrosit pada perlakuan (A) sebesar 500.000 sel/mm3, (B) sebesar 1.850.000 sel/mm3, (C) sebesar 1.110.000 sel/mm3 dan (D) sebesar 1.670.000 sel/mm3. Hasil pengamatan leukosit perlakuan (A) didapatkan sebesar 121.600 sel/mm3, (B) sebesar 333.550 sel/mm3, (C) sebesar 265.770 sel/mm3 dan (D) sebesar 145.620 sel/mm3. Berdasarkan hasil Imunohistokimia  didapatkan hasil pada perlakuan (A) nilai DAB sebesar 15,0%, (B) DAB sebesar 30,2%, (C) DAB sebesar 24,3% dan (D) DAB sebesar 19,2 %. Dari penelitian ini dapat disimpulkan bahwa pemberian deltamethrin berpengaruh terhadap eritrosit dan leukosit serta dapat menurunkan ekspresi CD4 sebagai respon imun pada ikan koi (Cyprinus carpio) yang terinfeksi Myxobolus sp. Parasites that often attack koi are Myxobolus sp. Fish infected with Myxobolus sp. will experience clinical symptoms of red nodules and swelling in the gill organs. The study aimed to determine the effect of deltamethrin administration on CD4 expression in koi fish (Cyprinus carpio) infected with Myxobolus sp. In this study divided into 4 treatments (A) Control, treatment (B) koi fish infected with Myxobolus sp., Treatment (C) koi fish infected with Myxobolus sp. by treatment a dose of 0.5 µl / g deltamethrin and treatment (D) koi fish infected with Myxobolus sp. with the treatment of 1 µl / g of deltamethrin. Then erythrocytes, leukocytes and immunohistochemistry were observed to determine CD4 expression in the intestine. Based on the results of the study obtained erythrocytes in treatment (A) of 500,000 cells / mm3, (B) of 1,850,000 cells / mm3, (C) of 1,110,000 cells / mm3 and (D) of 1,670,000 cells / mm3. Observation of leukocyte treatment (A) was obtained at 121,600 cells / mm3, (B) of 333,550 cells / mm3, (C) of 265,770 cells / mm3 and (D) of 145,620 cells / mm3. Based on the results of immunohistochemistry the results obtained in treatment (A) DAB value of 15.0%, (B) DAB of 30.2%, (C) DAB of 24.3% and (D) DAB of 19.2%. From this study it can be concluded that the administration of deltamethrin affects erythrocytes and leukocytes and can reduce CD4 expression as an immune response in koi fish (Cyprinus carpio) infected with Myxobolus sp.
ZOONOTIK BAKTERI Mycobacterium Tuberculosis YANG MENGIFEKSI IKAN GURAMI (Osphronemus gouramy Lac.) Rahmaningsih, Sri; Yanuhar, Uun
Jurnal Harpodon Borneo Vol 7, No 1 (2014): Volume 7 No 1 April 2014
Publisher : Jurnal Harpodon Borneo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.697 KB) | DOI: 10.35334/harpodon.v7i1.5

Abstract

Bakteri penyebab penyakit tuberculosis pada manusia dan hewan terutama ikan ini berbentuk batang ramping lurus atau sedikit  bengkok dengan kedua ujungnya membulat. Koloninya kering dengan permukaan berbentuk bunga kol dan berwarna kuning. Mycobacterium marinum berhubungan erat dengan Mycobacterium tuberculosis yang secara kompleks dapat menyebabkan penyakit pada ikan dan binatang amphibi dengan pathologi hampir sama dengan tuberculosis.  Selain dapat menyebabkan infeksi pada ikan, mycobacteria air secara signifikan termasuk  kedalam kelompok zoonotic. Produksi akuakultur Indonesia saat ini cukup tinggi yaitu sekitar 1,7 juta ton pada tahun 2005.  Salah satu komoditas yang menjadi prioritas dalam pembangunan subsektor perikanan budidaya ini adalah ikan gurami (Osphronemus gouramy Lac). .ikan gurame yang disebabkan oleh Mycobacterium sp.  Kejadian mycobacteriosis pada ikan gurame berkisar 30-60% dan penyakit ini dapat menyebabkan kematian hingga 70%. Penelitian ini bertujuan untuk mengetahui infeksi pada ikan gurami  yang disebabkan oleh bakteri Mycobacterium sp.Kata kunci: Mycobacterium sp, ikan gurame.
THE ANALYSIS OF HEPATOPANCREAS HISTOLOGYCAL DAMAGE IN NEOCALLICHIRUS KARUMBA (POORE AND GRIFFIN) SHRIMP CAUSED BY HEAVY METAL PB EXPOSURE IN MADURA STRAIT Kristiani, Maria; Herawati, Endang Yuli; Yanuhar, Uun
The Journal of Experimental Life Science Vol 6, No 1 (2016)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.006.01.06

Abstract

Madura strait known as the second busiest shipping lanes in Indonesia. Human activities on the environment can influence the marine ecosystem derived from the household, fishery processing and capture fisheries. It can pollute coastal environments, one of which is heavy metal exposure such as Cd, Hg, Ca, As and Pb. These metals are greatly impacting the life of sea biota. The objective of this research, therefore, is to examine the hepatopancreas damage rate of shrimp Neocallichirus karumba due to the exposure of heavy metal Pb in Madura Strait. Method of research is by analyzing Pb content in water, sediment and shrimp by taking sample from 3 different stations. Hepatopancreas damage is then analyzed with SEM-EDX. Result of research indicates that at Station A, Pb pollution in water is the biggest and counted for 0.25 ppm, while those in sediment and shrimp are 5.85 ppm and 1.24 ppm. At Station B, Pb pollution in water is 0.19 ppm, whereas that in sediment is 5.51 ppm, but that in shrimp is 1.04 ppm. At Station C, Pb pollution in water counts for 0.18 ppm, and in sediment, it stands for 5.5 ppm but 0.02 ppm for shrimp. Result of analysis against hepatopancreas damage is explained as follows. At Station A, vacuolization is 20 % and Pb content in organ is 0.520 ppm. At Station B, the parameters are 10% and 0.196ppm. At Station C, it includes 15% and 0.173ppm. Organ damage is straightforwardly related to Pb content in water and sediment. Shrimp age is quite influential to the percentage of organ damage.Keywords: Heavy metal Pb pollution, hepatopancreas organ damage, Madura Strait, N. karumba shrimp.
ASSESSING THE GENOTOXIC POTENTIALS OF METHOMYL-BASED PESTICIDE IN TILAPIA (OREOCHROMIS NILOTICUS) USING MICRONUCLEUS ASSAY Islamy, R Adharyan; Yanuhar, Uun; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol 7, No 2 (2017)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2017.007.02.05

Abstract

Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. The aim of this study to determine genotoxic effect of methomyl-based pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 - 96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12?, 18? and 16?; at concentration at 3.2ppm is 33?, 26? and 29?; at concentration at 4.2ppm is 41?, 38? and 46?; at concentration at 6.5ppm is 68?, 81? and 82?; at concentration 8.7ppm is 133?, 130? and 137?; at concentration 10ppm is 163?, 166? and 156?. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.Keywords: Genotoxic, Methomyl, Micronucleus Assay, Pesticide, Tilapia.
THE EFFECT OF CRUDE PROTEIN HALIMEDA SP. ON CYPRINUS CARPIO INFECTED KOI HERPES VIRUS ON EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-1 Yanuhar, Uun; Wahyuningtyas, Rika
JFMR-Journal of Fisheries and Marine Research Vol 1, No 1 (2017): JFMR
Publisher : JFMR-Journal of Fisheries and Marine Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.567 KB) | DOI: 10.21776/ub.jfmr.2017.001.01.3

Abstract

Halimeda sp. is a kind species of macroalgae that abundant grow in Indonesia. The utilization of Halimeda especially protein for disease prevention caused by the virus has not been done to the fish. The purpose of the study is to know the  treatment of crude protein Halimeda sp on  Cyprinus carpio infected by Koi Harvest Virus (KHV) on the expression of Major Histocompatibility Complex (MHC) class 1. MHC class-1 is one of the immune adaptive response on fish like this C. carpio. The methods are isolation of crude protein Halimeda sp,   purification  of  crude protein, Haemagglutination (HA) test and dot blot test. The result shows that the crude protein of Halimeda sp. can produce of adaptive immune response like MHC class 1. MHC class-1 has the function of  the immune system for maintenance of virus attacks directly. Responsible of treatment crude protein Halimeda sp. on C. carpio can be showed quantitatively using the dot blot test result. The  conclusion  is the crude protein of Halimeda sp. able to activate the MHC class I and to inhibit the proliferation of the KHV and maintenance of the fish cell of C. carpio..