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SPECIFIC PROTEIN EXPRESSION OF GOAT PREIMPLANTATION EMBRYO IN VITRO AS AN EARLY PREGNANCY SIGNAL Lanus Sumadiasa, I Wayan; Yuliani, Enny
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the experiment was to investigate the intensity of signal secreted by embryos for their survival in the early pregnancy. Embryos at morulla and blastocyst stages generated by in vitro fertilization were frozen and then thawed for in vitro culture in tissue culture medium. The protein secreted by embryo were detected 48 hours after thawing for those frozen at morulla stage, and 24 hours and 72 hours after thawing for those frozen in the blastocyst stage. Results showed that preimplantation goat embryos secreted an average of 3,041 µg protein per ml medium for the embryo cultured in tissue culture medium (TCM) 199 + 0,1 % BSA and 1,992 µg protein per ml medium for embryo cultured in TCM without BSA. Non growing embryos secreted only a small amount protein, i.e. 0,434 µg protein per ml medium and 0,417 µg protein per ml medium respectively for embryo cultured TCM 199 with BSA and TCM without BSA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed protein bands with many different molecular weight of 100, 95 , 55 , 43 , 28 and 18 KD. It appeared that the growing embryos secreted more proteins as compared to those non growing embryos.
SUBSTITUSI KUNING TELUR DENGAN LESITIN KEDELAI SEBAGAI PENGENCER SEMEN DALAM MEMPERTAHANKAN KUALITAS SPERMATOZOA KERBAU PENYIMPANAN 5°C Sabaan, Nifsu; Chairussyuhur Arman, Chairussyuhur; Yuliani, Enny; Maskur, Maskur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 3 (2019): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v6i3.8173

Abstract

ABSTRAKPengencer konvensional berbasis kuning telur yang bersumber dari hewan membawa risiko kontaminasi mikroba dan menghalangi jarak pandang pemeriksaan spermatozoa di bawah mikroskop. Penelitian ini dilaksanakan` untuk menilai apakah substitusi kuning telur dengan lesitin kedelai di dalam pengencer berbasis-tris dapat mempertahankan kualitas spermatozoa kerbau lumpur pada penyimpanan 5°C selama 120 jam. Ejakulat dari 3 ekor kerbau umur 3-4 tahun dengan bobot badan 450-500 kg ditampung dengan menggunakan vagina buatan.Semen diencerkan dengan pengencer konvensional yang mengandung 20% kuning telur (kontrol) dan pengencer lesitin dengan konsentrasi1%, 2%, 3% dan 4%(perlakuan) dan disimpan pada 5°C selama 120 jam. Parameter spermatozoa dinilai pada interval 0, 72, dan 120 jam setelah semen yang diencerkan disimpan 5°C.Persentase motilitas dan spermatozoa normal tidak berbeda nyata (P<0,05) antara pengencer 1% lesitin dan kontrol. Namun pengencer 1% lesitin mampu mempertahankan kedua parameter tersebut setelah penyimpanan 5°C selama 120 jam. Persentase viabilitas, keutuhan membran dan tudung akrosom spermatozoa pada pengencer 1% lesitin dibandingkan pengencer kontrol lebih tinggi (P<0,05) dari pengencer perlakuan 2, 3, dan 4% lesitin. Disimpulkan bahwa pengencer 1% lesitin dapat menyubstitusi pengencer konvensional berbasis kuning telur untuk penyimpanan semen kerbau pada 5ºC selama 120 jam.Kata kunci: kuning telur, lesitin, pengencer, semen cair, semen kerbauABSTRACTConventional egg yolk-based diluent animal origin carries the risk of microbial contamination and interference with microscopic examination. This study was conducted to assess whether substitution of egg yolk with soy lecithin in tris-based diluents can maintain the quality of buffalo spermatozoa stored at 5°C for 120 h. Ejaculate from 3 buffaloes aged 3-4 years and body weight of 450-500 kg was collected using an artificial vagina. Semen was diluted with diluent containing 20% egg yolk (control) and diluent with concentrations of lecithin 1%, 2%, 3% and 4% (treatment). Spermatozoa parameters were assessed at intervals of 0, 72 and 120 h after the diluted semen was stored at 5°C for 120 h. The percentages of motility and normal spermatozoa did not differ significantly (P<0.05) between 1% lecithin and control diluents. However, 1% of lecithin was able to maintain both parameters after stored at 5°C for 120 h. The percentages of viability, membrane integrity and functional acrosome of spermatozoa in 1% lecithin were higher (P<0.05) than control and the other three treatment diluents. In conclusion, 1% of lecithin diluent could substitute conventional egg yolk-based diluent for the storage of buffalo semen at 5ºC for 120 h.Keywords: buffalo semen, egg yolk, lecithin, diluents, liquid semen
Respon Tingkah Laku Birahi, Service Per Conception,Non Return Rate, Conception Rate pada Sapi Bali Dara dan Induk yang Disinkronisasi Birahi dengan Hormon Progesteron Mardiansyah, Mardiansyah; Yuliani, Enny; Prasetyo, Sugeng
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.452 KB) | DOI: 10.29303/jitpi.v2i1.24

Abstract

The aim of this study was to determine the differences ofestrous response, service per conception (S/C), nonreturn rate (NRR),and conceptionrate (CR) between Bali cattle heifersandcowssynchronized using intra-vaginalprogesterone hormone. Two groups of Bali cattle, 15 heifers and 15 cows, were used to examine the responsedifferences of estrous synchronization using progesterone hormone on reproductive performance, betweendifferent parity. Each group was given progesterone dose of 1 mg/kg body weight. The data observed wereestrousbehavior (estrousintensity, onset of estrous, estrous percentage), and value of S/C, NRR, and CR wereanalyzed by statistical analysis (t-test). The result shows the estrous intensity was significantly different (P&lt;0.05)between the heifers and cows. The average estrousintensity score of the heifer was 2.87±0.35, while the cows was2.27±0.45. There was significantly different (P &lt;0.05) in onset of estrous in the cows 41.5±5.18 hours, while inthe heifers was 50.67±3.5 hours. The estrouspercentage of the heifers and cows was the same, both of them was100%. There was no significant difference (P&gt;0.05) in S/Cvalue showed by the heifers (1,31±0,45)and the cows(1,50±0,50). The average value of NRR, for heifers and cowswas 86.67% and 80.00%, respectively. The averagevalue of CRfor heifers and cowswas 86.67% and 80.00%, respectively.
Efektifitas Kinerja Pentoksifilin Terhadap Kualitas dan Integritas Membran Plasma Utuh pada Sperma Sapi Bali Hasil Pemisahan dengan Menggunakan Albumin Rodiah, Rodiah; Yuliani, Enny; Dradjat, Adji Santoso; Arman, Chairussyuhur
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 1 No 1 (2015): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.932 KB) | DOI: 10.29303/jitpi.v1i1.12

Abstract

The purpose of this research was&nbsp; to determine the effect of Pentoxyfilline &nbsp;in maintaining the quality (motility, viability, morphology and plasma membrane intact) of Bali bull spermatozoa separated with albumine.&nbsp; A Completely Random Block Design was used in this study with two main factors (upper layer and bottom layer), diluents with four doses of pentoxyfilline (0, 15, 20&nbsp; and 25 ?M) with six &nbsp;replications. &nbsp;Evaluation of spermatozoa was performed by characterizing motility, viability, morphology, plasma membrane intact. Result of the study showed that the effect of four doses &nbsp;pentoxyfilline on the quality of Bali bull spermatozoa was non significant (p&gt;0,05). However, the addition of&nbsp; 25?M pentoxyfilline was found to give the best effect compared to 15 ?M, 20 ?M and control. Result&nbsp; of the study also indicated that spermatozoa separated in the upper layer was signifiqantly higher (p&lt;0,01) than that of separated in the bottom layer with the average &nbsp;motility, viability, morfology and&nbsp; plasma membrane intact was 66,67,80,50, 6,71 and 65,61% respectively.
Identifikasi Komponen, Faktor dan Resiko Relatif yang Berpengaruh pada Hasil Inseminasi Buatan pada Sapi Bali Dradjat, Adji Santoso; Rodiah, Rodiah; Yuliani, Enny; Arman, Chairussyuhur
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.266 KB) | DOI: 10.29303/jitpi.v2i1.26

Abstract

The aims of the research were to identify components, factors and relative risk (Odd ratio) which may influencethe artificial insemination results. The study were performed in two villages those were Kuranji and Tanah Beak,by identification of components and factors which may contribute to successful artificial insemination results,then the flow chart of successful and obstacle were designed. Based on the flow chart survey was performed to thefarmers and inseminators. Parameters evaluated were factors may influence of the result of insemination such as;management, oestrus detection by farmers and insemination by inseminators. The data was tabulated and analisedusing epidemiology analysis of odd ratios (OR). The results showed that insemination results of Kuranji villagedo not have the risk to be not pregnant as the odd ratio was less than 1 (OR &lt;1,0), with the range of 0,10 to 0,20.While artificial insemination in Tanah Beak village showed risk of cows to be infertile or non pregnant relativelyhigher with OR higher than 1 (OR&gt; 1.0), with the range of 1,5 to 2,0. The results showed that the risk to beunsuccessful to produce pregnancy were caused by oestrus detection, feeding management, skill inseminator andexperience of farmers. Finally it can be concluded that oestrus detection by farmers, feeding management,insemination by inseminator and experience of farmers may contribut to the high risk fail to produce pregnancyusing artificial insemination in Tanah beak village.
INTRODUKSI TEKNOLOGI INSEMINASI BUATAN PADA TERNAK KAMBING DI KECAMATAN BATU KELIANG UTARA LOMBOK TENGAH Sumadiasa, I Wayan Lanus; Zainuri, Lalu Ahmad; Yuliani, Enny; Arman, Chairussyuhur; Nugroho, Muhammad Prasetyo
Abdi Insani Vol 6 No 2 (2019): Jurnal Abdi Insani Universitas Mataram
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v6i2.206

Abstract

Populasi ternak kambing di Kecamatan Batu Keliang Utara Lombok Tengah tercatat hanya sekitar 873 ekor. Masyarakat Batu Kliang Utara menginginkan &nbsp;pengembangan ternak yang berbasis teknologi inseminasi buatan (IB), mengingat permintaan pasar akan ternak dan daging kambing yang semakin meningkat. &nbsp;Aplikasi teknologi pada ternak kambing belum berkembang karena sebagian besar mayarakat belum memahami cara penerapannya. Oleh karena itu, telah dilaksanakan pengabdian kepada masyarakat tentang ?introduksi teknologi IB pada kambing di Batu Keliang Utara, Lombok Tengah?. Tujuannya adalah memberikan pengetahuan dan keterampilan kepada masyarakat di Kecamatan Batu Keliang Utara tentang prospek, manfaat dan kelebihan teknologi inseminasi buatan (IB) pada ternak kambing. Metode pendekatan yang digunakan adalah penyuluhan dan pendidikan singkat dengan cara ceramah, diskusi dan memberikan contoh tentang hal-hal yang terkait dengan program IB dan upaya untuk meningkatkan efisiensi reproduksi dan produksi serta efisiensi usaha beternak kambing. Materi penyuluhan meliputi pemilihan pejantan dan betina, menadah sperma, menyiapkan betina, deteksi birahi dan peaksanaan IB, serta deteksi kebuntingan. Berdasarkan hasil evaluasi, kegiatan pengabdian kepada masyarakat dinilai cukup berhasil. Evaluasi yang dimaksud antara lain respon dan antusiasme peserta terlihat cukup besar. Para peserta tampak cukup memahami materi yang disuluhkan dan ingin menerapkan teknologi IB dalam kegiatan beternak yang bernilai ekonomis. Besarnya sambutan dan respon para peserta penyuluhan merupakan foktor pendukung yang cukup besar untuk diterima dan diterapkannya teknologi IB dalam manajemen reproduksi ternak mereka ke depan. Kesimpilan dari kegiatan pengabdian kepada masyarakat ini adalah diduga terjadi menambah wawasan dan pemahaman masyarakat tentang pentingnya penerapan teknologi inseminasi buatan dalam meningkatkan&nbsp; reproduktivits dan produktivitas &nbsp;ternak kambing.