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PENAMPILAN GALUR HARAPAN MUTAN DIHAPLOID PADI TIPE BARU DI SULAWESI SELATAN Dewi, Iswari Saraswati; Lestari, Endang Gati; ,, Chaerani; Yunita, Rossa
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.161 KB) | DOI: 10.24831/jai.v43i2.10408

Abstract

ABSTRACTSouth Sulawesi is known as one of national rice production centers. However, average productivity of rice varieties planted in that area (4.43 ton ha-1) is lower than those of rice productivity in Java (5.25 ton ha-1). The aims of this research were to evaluate agronomic characters and adaptation of 7 dihaploid mutant advanced lines of new plant type (DH-NPT) of rice at several locations in South Sulawesi. The research was conducted in 2012 at Maros, Gowa, Barru, and Pangkep. The experiments were conducted in randomized complete block design with 3 replications nested in locations. Treatment consisted of 7 DH-NPT of rice, i.e., BIO-MF115, BIO-MF116, BIO-MF125, BIO-MF130, BIO-MF133, BIO-MF151, BIO-MF153, and control varieties i.e., Fatmawati, Ciherang, and Inpari13. The results indicated that in general the lines had medium height (102.77-110.23 cm), moderate productive tiller (9-16 tiller per hill), moderate days to flower (50%), i.e., 73-76 days after sowing (DAS), earlier days to harvest (103-110 DAS), moderate panicle length (28.35-29.31 cm), large number of grain per panicle (> 250 grains) with moderate panicle fertility (63-70%), moderate 1,000 grain weight, i.e., 26.51-27.75 g, and high yield (7.51-8.09 ton ha-1). Four lines, i.e., BIO-MF116, BIO-MF130, BIO-MF151, and BIO-MF153 were stable and had wide adaptability. Other lines, i.e., BIO-MF125 and BIO-MF133 were sensitive to environmental changes, therefore they were classified as specifically adapted to favorable environment; while BIO-MF115 was not sensitive to environmental changes, and therefore it was adapted to non-favorable environment.Keywords: adaptation, agronomic characters, rice mutant
REGENERASI TUNAS DARI KALUS YANG TELAH DIBERI PERLAKUAN IRADIASI PADA PADI VARIETAS FATMAWATI [Shoot Regeneration of the Fatmawati Rice Variant Radiated Callie] Yunita, Rossa; Lestari, Endang G; Dewi, Iswari S
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.311 KB) | DOI: 10.14203/beritabiologi.v11i3.506

Abstract

Gamma ray mutative induction for increasing genetic variation has been applied for plant prime variety engineering. The materials are derrived from seed organ, shoot and calli. Calli is a group of actively dividing cell and have not been organized to form plant. The benefit of using calli explant is that the gamma ray could directly shot to DNA in the nuclear cell in such a way that there is higher opportunity for genetic change to occur. The problema of using calli explant are the difficulties in regenerating the calli into shoots, due to the deformation as a result of radiation process. Therefore, this research is aimed at obtaining the appropriate media formulation for shoot regeneration from Fatmawati-rice calli which has been irradiated with gamma ray. The reseach was conducted in BB-Biogen laboratory consisting of three experiments, such as : (1) calli iradiation with the dosage of 0; 5; 10; 15; 20; 25; 30; 35; 40; 45; 50; 55 and 60 Gy, (2) shoot regeneration at the MS + BA (0, 1, dan 3 mg/l) + IAA (0 dan 0,8 mg/l) media, and BA (0, 1, and 3 mg/l) + zeatin ( 0; 0,1; 0,2 and 0,3 mg/l) + IAA 0,8 mg/l and (3) Shoot induction at MS + IBA (0, 1, 2 and 3 mg/l) media. The result shows that the range of LD50 was obtained at the dosage of 30 Gy, the most apropriate media for shoot regeneration is MS + BA 3 mg/l + IAA 0,8 + zeatin 0,1 mg/l and media for root induction is IBA 1 mg/l.
PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF STAUROGYNE SP. IN FISH DISEASE BACTERIA Nugraha, Media Fitri Isma; Novita, Hessy; Rajamuddin, Muh Alias; Yunita, Rossa; Enggarini, Wening; Reflinur, Reflinur; Maharani, Fasya Hadaina; Elya, Berna
TORANI: Journal of Fisheries and Marine Science VOLUME 2 NOMOR 2, JUNI 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v2i2.7057

Abstract

This study was aimed to identify the potential use of active compounds extracted from Staurogyne sp as an antibacterial agent to control disease-causing bacteria in fish. Staurogyne sp, an aquatic plant collected from Bantimurung, South Sulawesi were subjected to allelochemical compound. Plant extracts were tested to five bacteria including four types of bacterial pathogen, such as Aeromonas hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, and Flavobacterium columnare, and one bacterial sensing quoroum, Chromobacterium violaceum. Based on phytochemical analysis, Staurogyne?s extracts derived from both stem and root contained flavonoids, phenols, and antioxidants compounds with the concentration of 0.018 mgQE/g, 0.3471 mgGAE/g, and  1004,391 IC50 µg/mL, respectively. In addition, Staurogyne plant extracts derived from both their stem and root has also produced both saponins and glycosides compounds. Plant extracts derived from Staurogyne?s leaves revealed three major chemical compounds such as flavonoids (0.77 mgQE/g), phenol (0.0629 mgGAE/g), and glycosides (+). However, no antioxidants and saponin compounds were detected.  Applying plant extracts as an antibacterial on five disease-causing bacteria causing diseases in aquaculture, such as Aeromonas Hydrophilla, Edwardsiella ictaluri, Streptococcus agalactiae, Flavobacterium columnare, and Chromobacterium violaceum bacterials revealed that at concentration of 0.1 g the Staurogyne sp extracts did not influence all the bacterials growth. However, by application of plant extract at concentration of 0.2 g on the growth media,three out of 5 tested bacterials (Aeromonas hydrophilla, Edwardsiella ictaluri, and Streptococcus agalactiae) showed intermediate inhibition responses on bacterial growth. The growth of remaining two bacterials pathogen, Flavobacterium columnare and Chromobacterium violaceum at 0.2 g plant extract of Staurogyne sp was not affected. This study revealed that the use of active compound derived from Staurogyne sp would be potential to be used in inhibiting disease-causing bacterial in fish in future. However, optimum concentration of the plant extracts, in particular on the inhibition of the growth of disease-causing bacteria in fish is still needed to adjust.Key words: Antibacterial, aquatic plant, Staurogyne sp, fish disease
STUDY OF AQUATIC PLANTS AND ECOLOGICAL- PHYSICS TEMPE LAKE, SULAWESI SELATAN Nugraha, Media Fitri Isma; Julzarika, Atriyon; Radjamuddin, Alias; Reflinur, Reflinur; Yunita, Rossa; Enggarini, Wening; Novita, Hessy
TORANI: Journal of Fisheries and Marine Science VOLUME 2 NOMOR 2, JUNI 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v2i2.7060

Abstract

Aquatic plants are an indicator of the fertility of an aquatic region. The waters of Lake Tempe are the largest waters of the lake area in South Sulawesi. Lake Tempe is located in the western part of Wajo District, precisely in Tempe District, about 7 km from Sengkang City towards the banks of the Walanae River in southern Sulawesi. The area is about 13,000 ha with a maximum depth of 5.5 m and can reach more than 30,000 ha during floods, and during the dry season, the inundation area reaches only 1,000 ha with a maximum depth of 1 m, located above the continental and Australian and Asian plates. This lake is one of the tectonic lakes in Indonesia. Every year silting the lake occurs. The Tempe hydro vegetation and eco-physical research were carried out in October 2017. The purpose of this study was to record aquatic plant species that live in Tempe Lake and observe ecological changes and physical properties of Lake Tempe. Aquatic plants are expected to be able to filter lake water. The results obtained are physical conditions of sharp-smelling water, unpleasant taste, dark brown, and cloudy color. Chemical indicators of NH3-N waters (0.2976-0.0634), PO4-P (0.0172-0.0844) NO2-N (undetectable), NO3-N (1.7131-1.9335), Sulphate (27.761900 - 37.047620), DO (6.88-7.18) and pH (7.88-8.02). There are 14 species of aquatic plants found in these waters. The most dominant species is water hyacinth. In the case of Tempe lake water vegetation results in siltation of the lake area.Keywords: Aquatic plant, Biodiversity, Species, Tempe lake,
PERBANYAKAN TANAMAN PULAI PANDAK (RAUWOLFIA SERPENTINA L.) DENGAN TEKNIK KULTUR JARINGAN Yunita, Rossa; Endang, Endang; Lestarai, Gati
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.308 KB) | DOI: 10.31258/jnat.14.1.68-72

Abstract

Due to over exploitation of its bark for medicinal herbs and made worse by problem in conventional breeding, Rauwolfia serpentina (Pulaipandak), has been considered rare and was currently reported to be an endangered species. Therefore, conservation measure is urgent to betaken. One of them is by in vitro propagation. In this research, in vitro propagation covers several activities, such as (1) shoot inductionwith the application of MS (Murashige and skoog) media enriched with ZPT 0.0; 0.1; 0.3 mg/l BAP combined with 0, 1, 2 mg/l 2ip, (2)shoot multiplication by using 0.0; 0.5; 1.0 mg/l BAP combined with 0.0; 0.1; 0.2 and 0.3 mg/l thidiazuron), (3) root induction IBA at theconcentration of 0.0; 0.5; 1.0; 1.5; 2.0; 2.5; 3.0 mg/l, and (4) acclimatization. The result showed that the best shoot induction for calli isthe in vitro stem by the application of MS + 0.3 mg/l BAP + 1 mg/l 2ip basic media. For shoot multiplication, the best media was MS + 0.5mg/l BAP + 0.1 mg/l ; while the best formula for root induction was MS + 1 mg/l IBA. The best media for plantlet acclimatization is compost+ soil mixture in 1:1 ratio.
KOMUNIKASI PENDEK Perbanyakan Tanaman Artemisia annua secara In Vitro Yunita, Rossa; Lestari, Endang G.
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

Artemisinin, an anti-malarial medicineisolated from the annual wormwood Artemisia annua,has a marked activity against chloroquine-resistant andchloroquine-sensitive strains of Plasmodium falciparum.This compound is useful for treatment of cerebral malaria.An in vitro propagation system for A. annua has been developed.Shoots were induced by culturing seeds of A.annua on a MS medium containing BAP (0, 0.1, 0.3, 0.5mg/l). Shoots were also formed on each seedling culturedon the same medium. Root formations were obtained fromshoots that were subcultured on a MS medium containingIBA (0, 1.0, 1.5, 2 mg/l). The results showed that MS mediumsupplemented with BAP 0.3 mg/l was the best medium forinduction and multiplication of the shoots, while the MSmedium supplemented with IBA (1 mg/l) was good for rootformations.
INDUCTION OF GENETIC VARIANT OF ARTEMISIA ANNUA L. WAS CONDUCTED THROUGH THE APPLICATION OF GAMMA RAY IRRADIATION IN 2007-2008. THE AIM WAS TO OBTAIN A PLANT WITH HIGH ARTEMISINE CONTENT > 0.5% AND LATE FLOWERING PERIOD OF ABOUT > 7 MONTH AFTER PLANTING. TWELEVE SELECTED GENOTYPES WERE SUBSEQUENTLY EXAMINED TO GAIN GENETIC STABILITY ON ALTITUDE OF 1500, 950, AND 540 M ASL. THE RESULTS SHOWED THAT THE PLANTS HAD SHORTER FLOWERING AGE IN CICURUG (540 M ASL) THAN THAT OF  IN PACET (950 M ASL ENDANG GATI LESTARI, ENDANG GATI LESTARI ENDANG GATI LESTARI; SYUKUR, MUHAMAD; PURNAMANINGSIH, RAGAPADMI; YUNITA, ROSSA; FIRDAUS, ROHIM
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.16

Abstract

Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and Gunung Putri (1540 m asl). Genotype 8 had the latest age of flowering in the three locations than the other genotypes, however, the growth and biomass were the lowest. Vegetative growth of Artemisia in Pacet and Gunung Putri was better than those in Cicurug. Genotype of 15 in Cicurug and 5A genotype in Gunung Putri and Pacet had higher wet and dry weight than that of two other associates. Based on plant biomass, 5 genotypes from Gunung Putri and Pacet i.e. 1D, 3, 5A, 14, and 15 genotypes were selected, as well as 5 genotypes i.e. 1D, 3, 4, 5A, and 15 genotypes from Cicurug. Analisys on artemisin content successfully obtained 5 selected somaclone lines i.e. 1B, 2, 4, 14, and 3 somaclones.
Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro Ariyanti, Febrina; Tumilisar, Christiani; Yunita, Rossa
Bioma Vol 10 No 1 (2014): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.873 KB) | DOI: 10.21009/Bioma10(1).5

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Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin
MULTIPLIKASI TUNAS MELINJO (GNETUM GNEMON) SECARA INVITRO Yunita, Rossa
Jurnal Sagu Vol 3, No 01 (2004)
Publisher : Jurnal Sagu

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Abstract

An experiment has been done at Laboratorium TLs.suc Culture of "Balai Penelitian Bioteknologi danGenctik Pertanian", Bogor, starting on July 2001 until June 2(M)2. The objective of the research is to studymultiplication of melinjo bud (Gnetum gnemon) by using in vitro technique. The experiment used factorialcomplete randomized design witli 19 replications. The thidiazuron were used as treatments at the concentra-tion of 0; 0,1 and 03 Ppm in combination with three kinds of medium i.e. MS, WPM and Anderson. The resultshowed that treatment of melinjo bud thidiazuron 0,3 ppra in MS medium could increase number of budmultiplication.
INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) Yunita, Rossa; Lestari, Endang Gati
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.807

Abstract

In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction.