Delita Zul
Jurusan Biologi FMIPA Universitas Riau Pekanbaru

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ENUMERASI TOTAL POPULASI MIKROBA TANAH GAMBUT DI TELUK MERANTI KABUPATEN RIAU Fitria, Rahmi; Zul, Delita; F, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Teluk Meranti is one of the peatland area in Riau province. Most of these lands have beenchanged into palm oil plantation, timber plantation, agricultural area and settlement. Theaim of this research was to analyze the impact of land use changes on soil physical-chemical characteristics and microbial cell number. Soil samples were taken from eightdifferent locations, namely primary forest as control, secondary forest, rubber plantation(15 monthsyears old), rubber forest (40-60 years old), palm oil plantation (7-8 years old),acacia plantation (2-3 years old), corn field, and cassava field. Microbial cell number wasdetermined by spread plate method, employing appropriate media for the growth ofbacteria, fungi and actinomycetes. The results showed that the soil humidity, soiltemperature, percentage of soil dry weight, water content, soil bulk density and pH rangedfrom 29,63-55,88%, 27-31,5 o C, 14,9-35,5%, 64,9-85,1%, 0,16-0,39 g/cm 3 and 3,63-4,00,respectively. The copiotrophic bacterial cell number ranged from 0,6x10 5 -1,8x10 5 CFU/gsoil where the highest population was at the palm oil plantation,whereas the oligotrophicbacterial cell number ranged from 0,5x10 5 -1,4x10 5 CFU/g soil where the highest populationwas at the palm oil plantation. The population of fungi ranged from 0,4x10 5 -1,0x10 5 CFU/gsoil where the highest population was at the corn field. The population of actinomycetesranged from 0,4x10 5 -10,7x10 5 CFU/g soil where the highest population was at the palm oilplantation. Land use changes caused microbial cell number increased. The results indicatedthat land use changes influenced the microbial cell numbers.
UJI EFEKTIVITAS BAKTERI PELARUT FOSFAT PENGHASIL ASAM SIANIDA ASAL TANAH GAMBUT RIAU DALAM MENGENDALIKAN GULMA DOMINAN PADA TANAMAN KELAPA SAWIT Septiani, Tri; Zul, Delita; Isda, Mayta Novaliza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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This research aimed to analyze the effectiveness of phosphate solubilizing bacteria (PSB) which are able to produce cyanide acid (HCN) in controlling of predominant weed in the palm oil plantation. This research was conducted from February to July 2014 in the Laboratory of Microbiology, Faculty of Mathematics and Natural Science, University of Riau. The effectiveness of PSB in controlling the growth of Mikania micrantha was performed by inoculating 26 selected PSB isolates into pre-germinated weed based on the Kremer and Souissi method modification. The parameters observed were root length and shoot length of seedling weed. The results showed that PSB isolates tested, in general, were effective to inhibit the growth of root and shoot length of the weed. BB_K9 isolate was the most effective in controlling the weed as it had a strong level of HCN production.
SELEKSI ISOLAT JAMUR DALAM MENGHASILKAN HORMON IAA (Indole Acetic Acid) ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR Astriani, Fenny; Fibriarti, Bernadeta Leni; Zul, Delita
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Indole Acetic Acid (IAA) is one of group of auxin phytohormones and plays a role to improve plant growth. Beside plants, fungi are also known to be able to produce IAA.This study aimed to select the ability of fungal isolates collection of Microbiology Laboratory, Faculty of Math and Natural Sciences University of Riau in IAA production. Fungal isolates were cultured in medium PDB (Potato Dextrosa Broth)enriched by tryptophan as a precursor of IAA. Indole acetic acid produced by fungi was detected by adding Salkowski reagent and determined quantitatively by the use of colorimetric method. The results showed that 47 fungal isolates were able to produce IAA. The highest IAA production was revealed by isolate RPL4-14 (646,75±0,35 ppm) and the lowest was shown by isolate RPL2-x (4,00±3,53 ppm) when they were culturedin PDB medium enriched by tryptophan. The fungal collection did not only produce IAA in the medium containing tryptophan, but also in the medium without tryptophan as shown by isolate RPL3-10, which has IAA concentration i.e 76,50±0,00 ppm and71,00±0,70 ppm, respectively. Isolates which produced the highest IAA concentration are identified as Penicillium sp.
APLIKASI MIKROBA LIGNOSELULOLITIK INDIGENUS ASAL TANAH GAMBUT RIAU DALAM PEMBUATAN KOMPOS DARI CAMPURAN TANDAN KOSONG DAN LIMBAH CAIR PABRIK KELAPA SAWIT (Elaeis guineensis Jacq.) Munawarah, Happy Zatul; Zul, Delita; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Activity of palm oil industries usually has side result such as empty fruit bunch (EFB) and palm oil mill effluent (POME) wastes. Those wastes still contain organic material that can be utilized as a substrate for compost production. Because of lignin and cellulose components, composting of EFB takes about 60 days to obtain standardized composts. It is known that composting process can be shorterned by adding bioactivators. The purpose of this research was to analyze the ability of indigenous lignocellulolytic microbes isolated from peat soil in Riau as bioactivator in composting of EFB enriched by POME. The selected isolates consisting of 4 bacteria (BB_S27, BB_HP42, BB_HP41 and BB_K20) and 2 fungi (LIJ1 and L1J2) were subcultured on Nutrient Broth and Potato Dextrose Broth. Starters were then made from a combination of the isolates and fermented during 7 days using seedling media. The compost treatments included negative control (seedling media without isolates), positive control by using effective microorganisms (EM) and bioactivator made in USA, and 4 combinations of those isolates resulting 7 treatments. Composting was done by the windrow composting system utilizing 400 kg EFB as a substrate and inoculated by 50 liters of the starters at the 1st and 14th incubation time. During 35 days composting process, the substrate was enriched by 20 liters POME every 2 days. The best quality compost was produced by treatment K3 (a combination of 4 bacteria and 2 fungi) as its characters almost in line with the National Quality Standard (ISO) such as N 2.22%, C/N ratio 14.5, P 0.760%, K 3.44%, blackish brown color, smell like soil and unraveled texture. The highest celullolytic and ligninolytic microbes cell number were also found in compost treated by K3 with value 8.8 x 108 CFU/g and 1.2 X 109 CFU/g, respectively.
SELEKSI KEMAMPUAN BAKTERI PELARUT FOSFAT ASAL BUKIT BATU-RIAU DALAM MENGHASILKAN ASAM SIANIDA Aprillia, Pristiana; Zul, Delita; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Phosphate solubilizing bacteria (PSB) is beneficial bacteria which are not only capablein solubilizing organic phosphate into inorganic phosphate, but also producing cyanideacid (HCN). HCN plays a role in controling weed growth. The objective of this researchwas to screen the potency of indigenous PSB isolated from peat soil in producting HCN.The collections of PSB were subcultured in King’B broth. The ability of 152 isolates onHCN production were tested qualitatively using filter paper soaked with picric acid andNa 2 CO 3 . The result indicated that as many as 26 isolates (17.1%) were able to releasevarious levels of HCN production based on the appearing of brown, dark brown, orreddish brown colour on the filter paper.
FORMULASI PUPUK HAYATI SERBUK MENGGUNAKAN BAKTERI PELARUT FOSFAT INDIGENUS ASAL TANAH GAMBUT RIAU DALAM BERBAGAI BAHAN PEMBAWA Haryanti, Dewi; Zul, Delita; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Peat soil has been known for its low content in phosphor (P). On the other hand, P solubilizing process is naturally slow. Inoculation of phosphate solubilizing bacteria (PSB) could increase this process. Therefore, the exploration of bacteria with such potential for use as biofertilizer agents is needed. Quality of biofertilizer depends on the viability and amount of bacteria contained, it is also influenced by type of carrier material. This research was aimed to find the appropriate carrier materials in production of biofertilizer which were contained PSB agent and to determine the quality of biofertilizer produced at a certain storage time. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2, and BB_HS13) were used to produce 3 starter combinations. The starters were prepared by growing the isolates in Pikovskaya’s media. Biofertilizer was produced by inoculating each starter into the peat and wood charcoal as carrier material and was fermented for 4 days. Biofertilizer quality was determined by calculating the PSB cells number during 0, 30, 60, and 90 days storage time and the degree of biofertilizer acidity. The cell numbers of starter I, II, and III ranged from 8.2.1010-2.9.1011 CFU/g, 5.3.1010-2.9.1011 CFU/g, and 8.0.1010-2.9.1011 CFU/g, respectively in a period of storage 0-90 days. The highest cell number at the end of storage was found from biofertilizer produced by using peat, as carrier material which was kept at 40C (1,3.1011 CFU/g, starter I), (8.6.1010 CFU/g, starter II), and (9.6.1010 CFU/g, starter III). Biofertilizer acidity was relatively neutral, 6.38. Based on the results obtained, peat was revealed as a good carrier material and the quality of biofertilizers which were produced was still good.
PENGARUH INOKULASI CAMPURAN ISOLAT BAKTERI PELARUT FOSFAT INDIGENUS RIAU TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN KEDELAI (Glycine max L. Merr) ALFIAH, LUFITA NUR; ZUL, DELITA; NELVIA, NELVIA
JURNAL AGROTEKNOLOGI Vol 7, No 1 (2016): Agustus 2016
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

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At this time the oportunity increasing soybean production still open through optimize the fulfill of the nutrient of plants. Phosphorus is one of element nutrient essentiality macro those as very important to growth and development of plants. The existence it in land abundance, but the concentration P who are get reserve by plant is very low. At acid land P bunching aluminum (AI) and iron (Fe) meanwhile in land Alkhalin P bounded calsium (Ca). The increasing efesiency of supply fosfat at plant can effort with exploit microorganism soluble phosphate group. Test potential solubility P by BPF isolated from peat soil Biosphere Reserve Giam Siak Kecil Bukit Batu, Riau. Test was conducted and taken in semi-quantitative which able to dissolve Ca3 (PO4) 2, FePo4 and phosphate rock. However adaptability and potential dilution BPF origin peat soils of Riau indigenus inoculated soybean in mineral soil until now unknown. This research was conducted in order to determine the effect of inoculation BPF indigenus Riau toward growth and yield of soybean. The study was conducted in factorial experiment using completely randomized design (RAL Factorial), as the first factor is soil treatment consisting of two levels ie: T0: soil without sterilization, T1: sterilized soil. The second factor is the provision isolates BPF which consists of 4 levels, namely: B0: without inoculation, B1: 2 isolates BPF (BB_UB6 and BB_K9), B2: 3 isolates BPF (BB_UB6, BB_K9 and BB_K2), B3: 4 isolates BPF (BB_UB6, BB_K9, BB_K2, and BB_HS13). The results showed soil sterilization decrease the number of primary branch becomes 5.67 and 1.49 g dry weight of the plant. The fastest time of harvest occurs in plants without inoculation of sterile soil that is 78.78. The highest weight of 100 seeds produced by the plant without inoculation of sterile soil that is 13.17 g.
POTENSI LIMBAH SAGU (Metroxylon sp.) DI KECAMATAN TEBING TINGGI BARAT KABUPATEN KEPULAUAN MERANTI SEBAGAI SUBSTRAT PENGHASIL BIOGAS Anuar, Khaidir; Zul, Delita; ', Fitmawati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Biogas is one of alternative energy sources that fulfills future fuel needs. This studyaimed to analyse the potency of sago waste as fermentation substrate for biogasproduction. This research was done experimentally by employing sago waste materialsobtained from Sago Factory Nambus River from District West Tebing Tinggi Sub-province Meranti and fresh cow rumen liquid was obtained from slaughterhouse inPekanbaru. The biogas volume was calculated by measuring the volume of water thatwas pushed every 3 days, where the volume of water driven was proportional to thevolume of biogas produced. The bacterial cell numbers was counted using plate countmethod employing Nutrient Agar (NA). The substrate fermentation temperature and pHwere measured every 3 days. The highest biogas volume was produced by fermenter III(45760 ml) containing solid sago waste, liquid sago waste, and rumen liquid with ratio 1: 1 : 1 and the lowest was produced by fermenter II (1600 ml). The total bacterialinvolved in biogas production ranges from 1,87x10 5 – 2,87x10 8 CFU/ml samples. Thetemperature and pH of substrate fermetation was relatively constant during biogasproduction. The results showed that sago waste is potential as substrate for biogasproduction.
Analisis Kualitas Air Lindi Asal Tempat Embuangan Akhir Sampah Kota Pekanbaru Berdasarkan Parameterbiologi, Fisika Dan Kimia DARYAT, FIKRI; ZUL, DELITA; L. FIBRIARTI, BERNADETA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Air lindi merupakan limbah cair yang berpotensi menimbulkan pencemaran lingkungan karena kandungan senyawa-senyawa yang bersifat toksik serta mikroorganisme patogen. Oleh karena itu, air lindi tidak boleh dibuang ke badan air tanpa penanganan terlebih dahulu dan diketahui kualitasnya. Penelitian ini bersifat deskriptif kualitatif yang bertujuan untuk analisis kualitas air lindi dari landfill TPA sampah Muara Fajar Kota Pekanbaru ditinjau dari aspek biologi, fisika, dan kimia. Sampel air lindi diambil dari 6 titik sampling yaitu pada parit pembuangan awal (inlet - PA), kolam proses pengendapan (K1), kolam proses anaerob (K2), kolam proses aerasi (K3), kolam proses penjernihan (K4) dan bak penampungan akhir (outlet - BPA). Parameter yang dianalisis meliputi pengukuran BOD, COD, pH, temperatur, NH3N, dan TSS yang ditentukan menggunakan metode standar. Selain itu juga dihitung indeks MPN menggunakan metode Most Probable Number dan deteksi keberadaan Salmonella dan Shigella menggunakan medium SSA. Hasil penelitian menunjukkan bahwa COD tertinggi dan terendah terdapat pada kolam PA dan kolam BPA dengan nilai berturut-turut yaitu 23.125 mg/l dan 3.750 mg/l. Nilai BOD tertinggi dan terendah pada kolam K3 dan kolam PA dengan nilai 1105,5 mg/l dan 393,4 mg/l. Nilai TSS tertinggi dan terendah pada kolam PA dan kolam K4 yang memiliki nilai 1,4 mg/l dan 0,7 mg/l. Indeks MPN tertinggi dan terendah pada PA, K1 dan BPA yang memiliki nilai ≥2400 dan <2. Salmonella sp. diketahui positif terdapat pada semua titik sampling, sebaliknya tidak ada Shigella sp. pada semua titik sampling. Nilai COD, BOD, dan indeks MPN dari air lindi untuk setiap titik sampling belum memenuhi baku mutu lingkungan, kecuali indeks MPN untuk kolam BPA telah memenuhi baku mutu lingkungan. Air lindi TPA Sampah Kota Pekanbaru belum dinyatakan layak sesuai dengan baku mutu lingkungan.
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.