ANIK KUSMIATUN
Department of Biology, Faculty of Mathemathics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680

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CHARACTERIZATION OF BACTERIOPHAGE SPECIFIC TO BACILLUS PUMILUS FROM CIAPUS RIVER IN BOGOR, WEST JAVA, INDONESIA KUSMIATUN, ANIK; RUSMANA, IMAN; BUDIARTI, SRI
HAYATI Journal of Biosciences Vol. 22 No. 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2344.168 KB) | DOI: 10.4308/hjb.22.1.27

Abstract

Bacillus pumilus is a spore-forming bacteria that is rod-shaped, gram positive, and aerobic. B. pumilus produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used to treat the disease caused by bacteria. Antibiotic typing test of B. pumilus indigenous from sewage water showed that this isolate was resistant to ampicillin and clindamycin. An alternative way was by application of bacteriophages as biocontrol agents to reduce B. pumilus in environment. The aim of this study were to isolate and characterize B. pumilus bacteriophage isolated from Ciapus River in Bogor, West Java. Bacteriophages infecting B. pumilus were isolated from river water using the double agar overlay method. Phages were defined by plaque morphology, structure, host range, and characteristic of molecular weight protein phage. Phage FBa1, FBa2, and FBa3 had narrow host range and they were specific for infecting B. pumilus. Electron microscope observation showed that phage FBa1 had icosahedral head without tail (166.67 nm in diameter), so it is called phage-like particles. Characterization of phage FBa1 by SDS-PAGE showed five proteins band. Molecular weight of FBa1 proteins was 70.9, 54.9, 33.8, 28.3, and 21.4 kDa.
PEMERIKSAAN MOST PROBABLE NUMBER (MPN) COLIFORM PADA ES BATU DI KELURAHAN KARANGSARI KECAMATAN NEGLASARI KOTA TANGERANG Kusmiatun, Anik; Fadillah, Arief
Jurnal Medikes (Media Informasi Kesehatan) Vol 2 No 2 (2015): November
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.142 KB)

Abstract

Konsumsi es batu diketahui menjadi sumber pembawa penyakit, timbulnya penyakit berkaitan dengan kurang diperhatikannya faktor kebersihan dan sanitasi dalam penanganan es batu. Hal ini menjadikan tingginya peluang kontaminasi mikrobiologis pada es batu. Oleh karena itu, evaluasi mengenai keberadaan coliform, menjadi penting untuk mengetahui tingkat sanitasi dan tingkat bahaya akibat mikroba patogen dari es batu yang dikonsumsi secara luas oleh masyarakat. Penelitian menggunakan metode deskriptif dengan desain penelitian pemeriksaan secara tidak langsung. Sampel yang digunakan adalah 5 Sampel es batu yang sudah dicairkan, diambil secara purposive sampel. Hasil Penelitian menunjukkan bahwa semua sampel pada uji penguat dinyatakan positif mengandung cemaran bakteri Coliform. Dari hasil yang didapat kualitas es batu di Kelurahan Karangsari Kecamatan Neglasari, Kota Tangerang tidak memenuhi persyaratan menurut SNI 7388-2009 bila ditinjau secara Bakteriologik.