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GENETIC DIFFERENCE BETWEEN TWO PHENOTYPICALLY SIMILAR MEMBERS OF ASTERACEAE BY THE USE OF INTERGENIC SPACER ATPB – RBCL Susanto, Agus Hery; Dwiati, Murni
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.637 KB) | DOI: 10.15294/biosaintifika.v11i3.22137

Abstract

Two Asteraceae species, i.e. Synedrella nodiflora (L.) Gaertn and Eleutheranthea ruderalis (Swartz) Sch.-Bpi. are phenotypically similar with each other, although some differences in morphological and anatomical traits are apparently observable. Molecular comparison using particular marker is required to support a phenotype-based study that previously reported. Chloroplast DNA marker, . atpB ? rbcL IGS, was used to identify genetic difference between both species. Six samples of the respective species were collected randomly from some places in Banyumas Regency, Central Java, Indonesia. Amplification of the marker was performed employing a pair of universal primers. Sequence alignment on the PCR products showed that no difference in atpB ? rbcL IGS sequences, either within S. nodiflora or E. ruderalis samples was observed. On the other hands, several deletions and base substitution in both S. nodiflora and E. ruderalis were detected when alignment was made between both species. This result suggests that they reveal a convincing genetic difference. Inspite of no direct correlation between this genetic and some visible phenotypic differences, this finding provides preliminary scientific background on the phenotypic traits of both species, which are often difficult to find at a rapid observation.
PERTUMBUHAN DAN HASIL TANAMAN CAISIN (Brassica chinenchis L.) PADA BERBAGAI JENIS PUPUK ORGANIK DAN PUPUK FOSFAT ALAM THE GROWTH AND PRODUCTION OF CAISIN (Brassica chinenchis L.) ON VARIETIES OF ORGANIC FERTILIZERS AND ROCK PHOSPHATE Kusumasari, Aryana Citra; Budisantoso, Iman; Dwiati, Murni
Pembangunan Pedesaan Vol 4, No 2 (2004)
Publisher : Pembangunan Pedesaan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tujuan penelitian adalah untuk mengetahui pengaruh pemberian pupuk organik dan fosfat alam terhadap pertumbuhan dan hasil tanaman caisin, serta menentukan jenis pupuk organik dan dosis pupuk fosfat alam terbaik dalam memacu pertumbuhan dan hasil tanaman caisin. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok dengan pola perlakuan Petak Terpisah (Split Splot Design). Petak utama berupa jenis pupuk organic yaitu pupuk kandang kambing (A ), pupuk kandang sapi (A ), dan pupuk 1 2 limbah media jamur merang (A ) dengan dosis masing-masing 10 ton/ha. 3 Anak petak berupa dosis fosfat alam 0 (B ), 250 (B ), 350 (B ), dan 450 (B ) 0 1 2 3 kg/ha, sehingga terdapat 12 kombinasi perlakuan dan setiap perlakuan diulang 3 kali. Parameter yang diamati meliputi parameter pertumbuhan yaitu jumlah daun, luas daun, bobot kering, nisbah pupus akar dan parameter hasil yaitu bobot basah daun. Data yang diperoleh dianalisis dengan uji F, apabila berbeda nyata dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa pemberian pupuk organik dan fosfat alam berpengaruh terhadap pertumbuhan dan hasil tanaman caisin. Pupuk limbah media jamur merang dan fosfat alam dosis 450 kg/ha (A B ) merupakan kombinasi terbaik dalam 3 3 memacu pertumbuhan dan hasil tanaman caisin.
Molecular Analysis of Synedrela Nodiflora (L.) Gaertn. Resistance Against Fomesafen using RAPD Markers Dwiati, Murni; Susanto, Agus Hery
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.290

Abstract

Both morphological and protein studies reveal that differences are observed between resistant Synedrella nodiflora against fomesafen and its susceptible wildtype. These are, however, more or less influenced by environmental factors, so that molecular analysis employing DNA markers is necessarily required. The methods involved total genomic DNA extraction using modified CTAB protocol following Doyle & Doyle (1990), RAPD marker amplification and visualization of RAPD markers prior to data analysis. Ten RAPD markers were used, but only seven of them showed polymorphism.  Calculation of genetic distance and variation was carried out employing PopGen software.Based on the RAPD markers used in this study, it can be concluded that genetic distance between susceptible and resistant S. nodiflora is higher than that within susceptible samples supporting our previous morphological and protein data, although genetic variation among susceptible individuals seems to be significantly high.
Pertumbuhan Stek Krisan (Chrysanthemum morifolium (L.) Ramat) pada Berbagai Media Kultur In Vitro Kristianti, Adven; Kamsinah, Kamsinah; Dwiati, Murni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.207

Abstract

Chrysanthemum (Chrysanthemum morifolium (L.) Ramat is one of ornamental plant commodities with high aesthetic value. As a consequence of increasing demand, the availability of seeds in adequate number is needed with method in vitro. This study aims to find out the effect of various in vitro culture media on the growth of chrysanthemum explants and to determine alternative media other than MS for in vitro growth of chrysanthemum explants. Experimental method arranged in a Randomized Complete Block Design (RCBD) with four treatments and four blocks is applied. The treatments are Murashige and Skoog  (MS), Vacint and Went  (V&W), Knudson  and   modified media, while node position serves as block. Time of shoot emergence, shoot length, number of leaves on each shoot, and number of roots are measured. Data are analyzed using Analysis of Variance (F test) with confidence intervals of 95% and 99%. Least Significant Difference (LSD) test is applied when significant difference among variables is observed. Culture media shows significant effect on chrysanthemum explant in vitro growth. Knudson media is found as an alternative media besides MS sufficiently suitable to increase chrysanthemum explants in vitro growth.
Aplikasi Paklobutrazol dan KNO3 untuk Meningkatkan Kualitas dan Kuantitas Bunga Potong Anggrek Dendrobium ‘Sarifah Fatimah’ Dwiati, Murni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 1 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.1.150

Abstract

A study on the effect of interactions between paclobutrazole and KNO3 application on the quality and quantity of Dendrobium ‘Sarifah Fatimah’ orchid cutflower was conducted. The experiment was arranged in a Randomized Complete Block Design (RCBD) employing two factors, i.e. paclobutrazole doses (100, 150, 200 ppm) and KNO3 doses (100, 200, and 300 ppm), replicated three times respectively providing 27 experimental units. The results showed that paclobutrazole and KNO3 application had significant effect on the reproductive parameters. To accelarate flowering and higher quality and quantity of the flowers, paclobutrazole of 100 ppm were applied at 2 and 5 weeks after adaptation combined with KNO3 of 100 ppm applied twice at interval of 3 weeks after paclobutrazole application could be given. 
Efek NAA dan BAP terhadap Pembentukan Tunas, Daun, dan Tinggi Tunas Stek Mikro Nepenthes ampullaria Jack. Sari, Heti Sartika; Dwiati, Murni; Budisantosa, Iman
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.343

Abstract

This study aimed to know the interaction between NAA and BAP as well as to obtain the best combination of both treatments in promoting the growth of Nepenthes ampullariamicrocutting.An experiment arranged in a factorial Randomized Complete Block Design (RCBD) wasapplied. Stem segments were used as blocks, where block I was the first segment followed by the next two segments as block II and III respectively. Two factors, i.e. NAA concentrations (0, 5, 10, 15 µM) and BAP concentrations (0, 9, 18, 27 µM) were employed giving rise to 16 combination of treatments. Each treatment combination was replicated threetimes resulting in 48 experimental units. The parametersmeasured were date of shoot initiation, date of root initiation, shoot number, leaf number, root number, length of longest leafand shoot height. The results showed that interactionbetween NAA and BAP in promoting N. ampullariamicrocutting growth was observed.  Combination between NAA of 0 µM and BAP of 18 µMwas found to be the best in promoting N. ampullariamicrocutting growth.Meanwhile, combination between NAA 0 µM and BAP 27 µM was recommended to promote shoot number of N. ampullaria.
Penambahan Ekstrak Alga Sargassum duplicatum Bory pada Medium Kultur In Vitro terhadap Pertumbuhan seedling Anggrek Vanda tricolor Lindl. Adiguna, Eko; Dwiati, Murni; Kamsinah, Kamsinah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.595

Abstract

Vanda tricolor is an orchid species that has many advantages, some of which are due to its beautiful and various flowers of unique shape and size. Propagation of V. tricolor can be performed by in vitro culture.In vitro culture technique needs appropriate medium to support orchid growth. An algae species, Sargassum duplicatum, can be added into the medium, since it contains growth regulating substances, such as auxin and gibberellin, which are useful for seedling growth. This study aims to know the effect of S. duplicatum application into in vitro culture medium of V. tricolor on the seedling growth and to know the best application of the algaein increasing seedling growth. An experimental method was arranged in a Completely Randomized Design (CRD). The treatments were S. duplicatum weight of 0, 12, 24, 36, 48 and 60 g/L replicated three times respectively. The variable examined was seedling growth with parameters comprising day of new leaf emergence, leaf number, leaf length, day of new root emergence, root number, root length and plant height. Data were analyzed using ANOVA or F test with confidence level of 95% and 99%. LSD was carried out when F test showed significant difference. The results reveal that application of S. duplicatum extract has significant effect on the growth of V. tricolor seedling. The best application is of 36 g/L algae weight.
Kandungan Bahan Aktif Fomesafen dalam Biji Kedelai dan Kacang Hijau dari Tanaman yang Ditumbuhkan pada Tanah Bekas Paparan Reflex Dwiati, Murni; Budisantoso, Iman
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.78

Abstract

A research has been conducted to understand the responses of soybean and mungbean to the persistence of Reflex residuals in soils referring to its active substance contents, i.e. fomesafen, in the plants grown in Reflex-exposed soils. The study was carried out experimentally in glass house from June to November 2003 employing Completely Randomized Design (CRD) with treatments arranged factorial. Two factors were used, the first of which was plant species (soybean and mungbean) while the second was Reflex concentrations (0, 25, 50, 100, and 200 ppm). Each treatment combination was replicated three times. The parameters examined comprised of fomesafen contents in the seeds, number of seeds per pod,  weight of 100 seeds, and weight of total seeds. To measure fomesafen contents in the seeds, Gas Liquid Chromatograph with ECD detector and column containing 10% OV-101 chromosorb WHF 80/100 mesh of 2m lengths and 3 mm diameter was applied. Fomesafen residuals in the soils of 5 month persistence had no harmful effect on both soybean and mungbean plants. As well, they had no significant effect on the production variables of both plants. Soybean seeds planted in the 5-month Reflex-exposed soils still contained fomesafen while the corresponding mungbean seeds did not.
Peran Paklobutrazol dan Pupuk dalam Mengendalikan Tinggi Tanaman dan Kualitas Bunga Krisan Pot Rochmatino, Rochmatino; Santoso, Iman Budi; Dwiati, Murni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.196

Abstract

The objective of this study was to determine the effect of paklopbutrazol concentration and fertilizer on plant height and flower quality of potted chrysanthemum. The experimental method was used with factorial design. The first factor was paklobutrazol with the concentration as follow:  0, 100, 200, and 300 ppm. The second factors were cow, chicken, and goat manure. Parameters observed were plant height, time of flowers emerging, flower diameter and freshness. The results showed that certain concentration of paklobutrazol might inhibited plant height, time of flowers emerging, and shortening of flowers freshness, while chicken manure fertilizer spurred flower diameter.
Serapan Fosfor dan Pertumbuhan Kedelai(Glycine max) pada Tanah Ultisol dengan Pemberian Asam Humat Wahyuningsih, Wahyuningsih; Proklamasiningsih, Elly; Dwiati, Murni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.911 KB) | DOI: 10.20884/1.mib.2016.33.2.345

Abstract

Ultisol merupakan salah satu jenis tanah asam yang memiliki kelarutan Al, Fe, dan Mn tinggi, serta kandungan P dan Moyang rendah. KelarutanAl dan Fe yangtinggiakan menjerap fosfat, sehingga ketersediaan dan serapan P bagi tanaman menjadi rendah. Salah satu upaya untuk mengatasi ketidaktersediaan P pada tanah Ultisol yaitu dengan penambahan asam humat. Asam humat merupakan makromolekul polielektrolit yang memiliki gugus fungsional seperti -COOH, -OH fenolat, maupun -OH alkoholat,sehingga asam humat memiliki kemampuan untuk membentuk kompleks dengan ion logam. Penelitian ini dilakukan dengan tujuan untuk: (1) mengetahui pengaruh asam humat terhadap serapan P dan pertumbuhan tanaman kedelai (2) menentukan konsentrasi asam humat yang paling baikdalam meningkatkan serapan P dan pertumbuhan tanaman kedelai. Metode penelitian yang digunakan adalah eksperimental dengan Rancangan Acak Kelompok (RAK). Pengelompokan didasarkan atas perbedaan intensitas cahaya yang ada di rumah kaca. Perlakuan berupa konsentrasi asam humat, dengan 5 konsentrasi yang berbeda yaitu 0 ppm, 400 ppm, 800 ppm, 1200 ppm dan 1600 ppm. Parameter yang diukur meliputi tinggi tanaman, luas daun, bobot basah, dan bobot kering, P tersedia serta P tanaman. Data yang diperoleh dianalisis menggunakananalisis ragam pada tingkat kepercayaan 95% dan 99%. Apabila hasil perlakuan berpengaruh nyata, maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa asam humat berpengaruh terhadap serapan P dan pertumbuhan kedelai pada tanah Ultisol. Asam humat 1200 ppm merupakan perlakuan paling baik dalam meningkatkan ketersediaan P, sehingga mampu meningkatkanpertumbuhan kedelai pada tanah Ultisol.