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OPTIMIZATION AND PERFORMANCE TEST OF OIL SPILL DISPERSANT (OSD) AT BIOREMEDIATION OF CONTAMINATED SOIL WITH HEAVY OIL BY BIOSLURRY TECHNIQUE Aziz, Muhammad Abdul; Yani, Mohamad; Syakti, Agung Dhamar
Journal of Tropical Soils Vol 25, No 1: January 2020
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2020.v25i1.%p

Abstract

Petroleum industry activities produce waste such as petroleum hydrocarbons which damage soil environment due to changes in soil physical, chemical and biological properties. Oil Spill Dispersant (OSD) is a product that can break down waste of oil into small parts so that it can be dispersed naturally. Laboratory experiments aimed to find out optimize and performance test of OSD in the process of bioremediation with using bio-slurry technique on contaminated soil with heavy oil carried out at Laboratory of Surfactant and Bio-energy Research Center (SBRC), Research and Community Service Institute of Bogor Agricultural University on January - August 2018 using contaminated soil with heavy oil. The experiment used Response Surface Method (RSM) with two factors, namely the incubation time factor (X1) and the Dispersant to oil ratio (DOR) (X2). The observed variables were soil Total Petroleum Hydrocarbons (TPH), pH, total microbes, and Chemical Oxygen Demand (COD) at soil solution. The results showed that the treatment of time and its combination with DOR significantly reduced soil TPH, increased soil acidity, and increased soil total B. megaterium, but did not significantly effect on COD in soil solutions. Optimization of OSD with RSM showed that the higher DOR of OSD and the longer the time of bioremediation, the higher also the rate of biodegradation of TPH. The optimum conditions were reached at DOR of 1.16:1 and bioremediation time of 7 days witch were able to degrade soil TPH of 54.30%. The optimum conditions of soil pH (8.825) was reached at DOR of 1:1 and bioremediation time of 5 days, as well as the optimum conditions of B. megaterium (8.35 log CFU g-1) was reached at DOR of 0.86:1 and bioremediation time of 7 days. Oil spill dispersant (OSD) increased COD at soil solution in both uncontaminated and contaminated soils with heavy oil.
BIOSURFAKTAN BAKTERI HIDROKARBONOKLASTIK DALAM PROSES BIOTRANSFORMASI POLYAROMATIC HYDROCARBONS (PAHS) : POTENSI AKSELERASI BIODEGRADASI PAHS Hidayati, Nuning Vita; Syakti, Agung Dhamar; Darmayati, Yeti
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Capability of Rhodobacterium bacteraceae in producing biosurfactant were investigated. Biosurfactant has been produced using medium of Mineral Salt Medium with two sources of carbon, which is crude oil (RB-CO) and waste coconut oil (RB-WCO) as carbon sources. Biosurfactant extent was determined by the yield of biosurfactant and the surface tension reduction. Biosurfactant were investigated for their efectiveness on PAHs biodegradation. The results showed that R. bacteraceae can produce biosurfactant, and showed the highest biosurfactant yield (2,791 g/l) when grown on RB-WCO, while we found 2,122 g/l yield on MSM-CO after 72 hours. The best biosurfactant activity was obtained when using waste coconut oil as carbon source, resulted in surface tension reduction up to 29,17 mN/m. Addition of 50 mg of biosurfactant per 200 ml of the mineral salt medium containing 50 ppm PAHs significantly enhanced the PAHs degradation indicating that the organism utilized crude oil as carbon source. Our concluding remarks suggest that biosurfactant from R. bacteraceae when grown on waste coconut oil substrate can be used as an effective agent to be applied on site contaminated with PAHs by enhancing biodegradation rate.
The Distribution of Lead Accumulation (Soil, Water and Mangrove Vegetation) to Conserve Segara Anakan Lagoon Hilmi, Endang; Siregar, Asrul Sahri; Syakti, Agung Dhamar
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.688 KB) | DOI: 10.20884/1.oa.2017.13.2.83

Abstract

Segara Anakan Lagoon  is a  specific lagoon as dispose industry waste area, i.e. lead waste.  Distribution of lead accumulation in vegetation, soil and water can explain pollution status in Segara Anakan Lagoon.  This study aims to analysis lead accumulation distribution  (soil, water and vegetation),  bioaccumulation factor (BAF), translocation factor (TF), leaf morphometric (the lead effect for mangrove vegetation), and to develop pre-design a mangrove  zoning based on lead accumulation. The results showed that lead accumulation in Segara Anakan Lagoon (SAL) was 0.177 – 0.233 mg/l (water),  0.320 – 0.780 mg/kg (soil),  4.80–8.67 mg/kg (mangrove roots), for stem between 2.48–6.96 mg/kg (mangrove stem), and 1.48–4.76 mg/kg (mangrove leaf).  BAF of mangrove vegetation scored between 22.2–40.1 and TF between 0.9–1.3. The lead accumulation of soil depths were 8.89 mg/kg (0-50 cm) to 0.56 mg/kg (150-200 cm). The effect of  lead accumulation was observed as leaf damage between 2-60 % of surface leafs. And based on best lead accumulation of mangrove vegetation showed that the first mangrove zoning was Rhizophora apiculata, Bruguiera sexangula, Aegiceras corniculatum and  Sonneratia caseolarisKeyword: lead accumulation; mangrove capacity; bioaccumulation factor; translocation factor;  lead effect
ASSESSMENT OF CADMIUM (Cd) CONTAMINATION IN MUD CRAB (Scylla spp.) AND SEDIMENT FROM SEGARA ANAKAN LAGOON, CILACAP, INDONESIA Susanto, Feri; Hidayati, Nuning Vita; Syakti, Agung Dhamar
Journal Omni-Akuatika Vol 10, No 2 (2014): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.209 KB) | DOI: 10.20884/1.oa.2014.10.2.18

Abstract

The study aimes to examine the accumulation level of selected heavy metal Cd in various of common mud crab (Scylla spp.) samples from Segara Anakan Lagoon-Cilacap. Mangrove crab and sediment samples were taken from Segara Anakan (108046’–109005’E; 7034’–7048’S) and were analyzed using Atomic Absorption Spectrophotometer (AAS). The Cd extent in Scylla spp. varied from 0.084 - 0.273 ppm which still below the European Commission (2006) threshold value (0.5 ppm). On the other hand Cd concentration in sediment for most stations value (0.625 - 1.635 ppm) have exceeded the Interim marine sediment quality guidelines (ISQGs value; 0.7 ppm) except for station 2 and 4. Concerning the sediment, to interpret and assess the contamination status four indices were used, namely Contamination Factor (CF), Enrichment Factor (EF), Pollution Load Index (PLI), and Geoaccumulation Index (Igeo). The results show the Segara Anakan sediment value for CF varied 3.125 - 8.175, EF was 50.481, PLI was 5.381 and Igeo varied 0.319 - 0.736. The finding result shows the Segara Anakan sediment could be considered as moderately polluted.Keywords: cadmium, contamination, scylla, sediment.
HEAVY METALS (Ni, Cu, Zn AND Cd) CONTENT IN SERUM OF RAT FED GREEN MUSSELS Azis, Muhammad Yudhistira; Gandasasmita, Suryo; Syakti, Agung Dhamar
Journal Omni-Akuatika Vol 11, No 2 (2015): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.264 KB) | DOI: 10.20884/1.oa.2015.11.2.33

Abstract

Green mussel (Perna viridis) can playing role as bio-indicator or biomonitoring agent for heavy-metalcontaminations in the sea. In this research, the concentrations of four elements Ni, Cu, Zn and Cd in P. viridis and in the serum of rat which orally feed by P. viridis were determined by Atomic Absorption Spectrometry (AAS) following dry acid digestion. Parameter analysis was evaluated by determining confidence limit for the obtained results. The result showed that there was a sequence of heavy-metal content in green mussels sample and laboratory rats serum, such as Ni < Cd < Cu < Zn.Keywords: heavy metals, green mussels, laboratory rats serum, AAS
Front-Matter for 13 (2): 1-198 Syakti, Agung Dhamar
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

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Abstract

Microplastics Monitoring in Marine Environment Syakti, Agung Dhamar
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.164 KB) | DOI: 10.20884/1.oa.2017.13.2.430

Abstract

This review summarizes the need for future spatiotemporal comparisons of microplastic abundance across marine environment, through standardized methods for microplastic sampling and analysis in sea water, beach and seabed sediment and marine organism. Pretreatment of the sample prior to the elimination of organic matter should be done using appropriate reagents was also described. Extraction of microplastics from environmental matrices is based on the different density of targeted microplastics with saturated salt solutions (NaCl, NaI, CaCl2, ZnCl2 and lithium metatungstate). Quantification can be achieved by microscopic techniques (binocular, stereomicroscope, fluorescence microscope and scanning electron microscope) and discussion on identification methods including FTIR, Pyr-GC/MS and Raman spectroscopy will be provided. This review also endorses the importance of further study regarding the fate and impact of microplastics on marine biota and human health, especially when we acknowledge that co-pollution may occur during the transport on microplastic in marine environment.
KAPASITAS ASIMILASI BEBAN PENCEMARAN DI PERAIRAN TELUK RIAU Idris, Fadhliyah; Koenawan, Chandra Joei; Jumsurizal, Jumsurizal; Syakti, Agung Dhamar
Dinamika Maritim Vol 6 No 2 (2018): Dinamika Maritim, Vol. 6 No. 2, February 2018
Publisher : Coastal and Marine Resources Research Center, Raja Ali Haji Maritime University, Tanjungpinang, Indonesia

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Abstract

Riau bay, Tanjungpinang City has been subject for domestic wastes contamination due to the high number of population living in the coastal area. This study aims to determine water quality condition including the pollutant load and the level of assimilation capacity in Riau bay waters. Using a survey method by purposive sampling strategy, the research was conducted by analyzing several physic-chemicals parameters. The results showed the measured physical parameters was still below the threshold level stipulating Minister Decree No. No 51 of 2004. Pollutant loads represented by BOD5, TSS&nbsp; and ammonia were 12,71 mg/L, 239,95&nbsp; mg/L and 5,33 mg/L respectiely. Our finding showed the assimilation capacity level for BOD5, TSS&nbsp; and ammonia were 159,26 ton/year, 530,98 ton/year and 0,86 ton/year, respectively.
ANALYSIS OF CAROTENOIDS AND THE IDENTIFICATION OF MANGROVE SEDIMENT BACTERIA OF SEGARA ANAKAN, CILACAP Riyanti, Riyanti; Rahmawati, Meris; Hidayati, Nuning Vita; Syakti, Agung Dhamar; Radjasa, Ocky Karna
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 4 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

The excessive consumption of artificial dyes can lead to negative effects on human body. Thus, the invention of natural dyes, such as carotenoids, is needed in order to reduce the negative impacts. Carotenoids are yellow, orange, and reddish yellow pigments produced by plants, animals, algae, and microorganisms. This study was aimed to analyze the carotenoid pigments of mangrove sediment bacteria in Segara Anakan, Cilacap, and to identify species of bacteria that can produce carotenoids. Carotenoids were analysed by using Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer. Meanwhile molecular identification of bacteria was carried by 16S rDNA PCR and DNA sequence was analysed through a program called Basic Local Alignment Search Tool (BLAST). The kinship of bacteria was shown in Phylogenetic tree by using Bioedit and MEGA 5 software. Qualitative analysis by using TLC produced several pigments like: ?-carotene, ?-isorenieraten, lycopene, flavonoids, chlorophyll a, chlorophyll b and a feofitin with Rf value of 0.36-0.95. Quantitative analysis showed that bacteria KH (greenish yellow), KT (dark yellow) and KM (light yellow) produced carotenoids at 95.30 mg.g-1, 110.34 mg.g-1 and 25.349 mg.g-1. KH, KT and KM were suspected to be bacteria known as Streptomyces chartreusis, Bacillus megaterium and Streptomyces chromofuscus with similarity of 99%. Mangrove sediment bacteria had the potential of producing carotenoids as an alternative of eco-friendly natural dyes.
Marine Bioremediation in Indonesia : Die Before Blossom Syakti, Agung Dhamar
Journal Omni-Akuatika Vol 14, No 3 (2018): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.097 KB) | DOI: 10.20884/1.oa.2018.14.3.584

Abstract

This critical review highlighted the need to booster an application of the environment biotechnology in order to reduce petroleum hydrocarbons contamination in marine environment.  Marine bioremediation can be promisingly applied when the spills occurred. However, absence of policy support from Indonesian government to endorse the private actor’s liabilities when complying the rehabilitation action was no significance while academic research was still far from establishment of fundamental knowledge in marine bioremediation. Thus, if so, it very likely that bioremediation disciplines and application might “die before blossom” in a country that often confronted by the maritime risk contamination of petroleum hydrocarbons on their marine and coastal area.