Yuni Kilawati
Aquatic Resources Management Department, Fisheries and Marine Science Faculty, University of Brawijaya

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HEAVY METAL PB CONTENT IN THE SEAWATER, SEDIMENT AND GREEN MUSSEL TISSUE PERNA VIRIDIS Haryono, M. Gandri; Mulyanto, .; Kilawati, Yuni
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 1 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.162 KB) | DOI: 10.29244/jitkt.v9i1.17864

Abstract

Accumulation of heavy metal Pb into the tissue can proceed through a food chain or environmental exposure. This study was to determine the content of heavy metals Pb in water, sediments and mussels (perna viridis). This research was conducted in March 2016, in the waters of Lekok Pasuruan at the three stations. TPI Station 1, Station 2 at the mouth of the Rejoso river and station 3 nearby PLTU 3. The water, sediments and green mussels (Perna viridis) samples were collected for Pb analysis using Absorpotion Atomic Spectrophotometer (AAS). In addition to the water quality such as salinity, temperature, DO and pH was observed. The results showed the highest Pb content in the water (0.4444 mg/l), sediment (23.8284 mg/kg) and green mussel tissue (1.5098 mg/kg) were found at station 2 (Rejoso river mouth). The Pb content in green mussel seemed to above safety level to consume.
Dampak Stres Salinitas Terhadap Prevalensi White Spot Syndrome Virus (WSSV) dan Survival Rate Udang Vannamei (Litopenaeus vannamei) Pada Kondisi Terkontrol Amrillah, Attabik Mukhammad; Widyarti, Sri; Kilawati, Yuni
Research Journal of Life Science Vol 2, No 2 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (806.052 KB) | DOI: 10.21776/ub.rjls.2015.002.02.5

Abstract

White spot syndrome (WSS) adalah penyakit yang secara signifikan menyebabkan tingginya mortalitas dan kerusakan parah pada budidaya udang. Penelitian ini bertujuan mengetahui dampak stres salinitas terhadap prevalensi WSSV dan survival rate udang vannamei (Litopenaeus vannamei). Penelitian ini menggunakan udang vannamei ukuran PL 40 yang diinfeksi WSSV dengan konsentrasi virus 20 μg/ml pada tiga rentang salinitas yang berbeda 0-10 ppt, 11-20 ppt, 21–30 ppt dan di rendam selama 4 jam kemudian dilakukan pengamatan selama 7 hari pasca infeksi dan diukur survival rate dan kuaitas airnya. Hasil pengamatan menunjukkan bahwa seluruh sampel terinfeksi oleh WSSV, ditunjukkan hasil analisa PCR dan gejala klinis yang timbul. Salinitas 0-10 ppt memberikan hasil persentase survival rate terendah jika dibandingkan dengan rentang salinitas yang lainnya yaitu sebesar 7 ekor atau 33% dari jumlah total sampel yang digunakan. Persentase survival rate udang tertinggi pasca infeksi virus WSSV terdapat pada perlakuan salinitas 21-30 ppt yaitu sebesar 13 ekor atau 63% dari jumlah total individu, dan salinitas 11-20 ppt memiliki persentase survival rate medium yaitu sebesar 10 ekor atau 49% dari jumlah total sampel. Stres salinitas mempengaruhi prevalensi WSSV dengan semakin tingginya tingkat infeksi seiring menurunnya rentang salinitas, akan tetapi survival rate semakin tinggi seiring dengan bertambahnya rentang salinitas.
Analysis of Heavy Metal Pollutant in Wangi River Pasuruan and Its Impact on Gambusia affinis Adam, Mohammad Awaludin; Maftuch, Maftuch; Kilawati, Yuni; Tahirah, Siti Nur; Risjani, Yenny
Indonesian Journal of Environment and Sustainable Development Vol 9, No 2 (2018)
Publisher : Graduate Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2018.009.02.09

Abstract

Pollution that occurred in Pasuruan area’s watershed Wangi–Beujeng river, District of Beji, Indonesia has been initiated in 2007 and continues to this day. The activity was caused by many factors such as the industrial (I), household wastes (II) and agriculture wastes (III) as well as the erosion process.The aims of this study to analyzed the heavy metal pollution that occurs in the Wangi river flow and the effect of gill histology and antioxidant activity on gambusia fish. The research method of observation at river flow and sampling for laboratory test.Three sites were assigned for chemical sampling and tissue histologycal in this study. Fish (Gambusia affinis, local name: Gatul) and water were used as indikatorfrom each site to determine of cadmium (Cd), lead (Pb) and mercury (Hg) concentration using Atomic Absorption Spectrophotometry (AAS). The study was indicated that the streams previously used by residents for daily activities. Results showed level of cadmium (Cd), lead (Pb) and mercury (Hg) in Gambusia affinis exceeded the permissible standard (0.01 ppm Cd;0.03 ppm Pb;0.001 ppm Hg)respectively. Based on histologycal, the tissue showed of damage ofchloride cell (CC) which was used in ion homeostasis process and heavy metal route in grill fish. While protease activities, CAT, MDA and peroxidasse was increased in each sampling area, with significant different (>0.95) between the three sampling sitesKeywords: Chloride cell, Heavy metal, Toxicity, Wangi river 
ICP11 as Biomarker for WSSV Disease in Litopenaeus vannamei Kilawati, Yuni; Maimunah, Yunita; W. Ekawati, Arning
Research Journal of Life Science Vol 2, No 3 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.35 KB) | DOI: 10.21776/ub.rjls.2016.002.03.5

Abstract

Environmental pollution could directly reduce water quality for shrimp cultivation. This study aims to determine how the environmental quality, shrimp population and genetic characteristics of shrimp that live in polluted waters in shrimp ponds in East Java associated with WSSV disease that often attacks on cultivated shrimp. The method used was a descriptive exploratory. Data collection was collected by observation and interview with farmers on disease history. Water was sampled for water quality study and characterize its suitability for shrimp cultivations. Shrimps were also sampled for morphological and genetical study for its susceptibility of WSSV. All samples were taken every week during shrimp cultivation in Malang ans Gresik. Morphological study using scoring method to determine the disease stages on shrimp, while for genetical study using specific primer for ICP11 for WSSV detection, since ICP 11 is expressed when WSSV infection is occur. Samples were taken from 2 shrimp ponds in South Malang and Gresik.The overall water quality is good, except for NO2 and TOM in both seawater shrimp ponds which higher than the freshwater/estuary shrimp ponds. Light infections of WSSV were detected in all seawater ponds both in morphology and genetics. However, in the freshwater/estuary pond only shrimps from freshwater/estuary Gresik which showing light WSSV infection genetically, but not in the morphological signs. Early disease detection is important to control the disease spread.
Genetic Characteristics Of White Shrimp (Litopenaeus Vannamei) In Banyuwangi And Situbondo: The Infected White Spot Syndrome Virus (WSSV) kilawati, yuni; Maimunah, Yunita
Journal of Food and Life Sciences Vol 2, No 2 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6.551 KB)

Abstract

Water pollution caused by human anthropogenic activities has deteriorating effect on water quality for aquaculture. Therefore, the objectives of this research were to determine how water quality, population and shrimp genetic characteristic in polluted area. Especially in East Java which have high virulency on WSSV outbreaks.Data exploration using surveillance technique was collecting physical and chemical water quality parameters. Shrimp samples were analyzed for morphological and genetical characteristics using specific WSSV primer ICP 11.Water quality at all sampling locations were categorized as polluted refers to ammonia and TOM  concentrations. Low water quality acts as a trigger for WSSV outbreaks. Shrimp pond from Situbondo exhibited as carrier indicated by the presence of WSS infection based on genetic characteristics, while morphological characteristics did not.
Kualitas Lingkungan Tambak Insentif Litapenaeus Vannamei Dalam Kaitannya Dengan Prevalensi Penyakit White Spot Syndrome Virus Maimunah, Yunita; Kilawati, Yuni
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.254 KB) | DOI: 10.21776/ub.rjls.2015.002.01.7

Abstract

Increasing number of vannamei shrimp (Litapenaeus vannamei) ponds are switching from traditional to intensive farming systems, the more impact resulting among other potential environmental pollution. Pollution of the environment can directly degrade water quality cultivation and facilitate access of pathogens to infect the host. In this study examines how the quality of the environment, population and genetic characteristics of shrimp that live in some intensive pond associated with a disease that often affects farmed shrimp is White Spot Syndrome Virus (WSSV). Acquisition and primary data collection is done by conducting interviews and direct observation in the measurement of water quality parameters of both physics and chemistry and morphology observation of shrimp as well as the ICP11 gene expression detection of WSSV disease in vannamei shrimp DNA in the laboratory.
Activity of Compounds on Seaweed Eucheuma cottonii Extract as Antioxidant Candidate to Prevent Effects of Free Radical in Water Pollution Wulandari, Dewi; Kilawati, Yuni; Fadjar, Mohamad
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.638 KB) | DOI: 10.21776/ub.rjls.2018.005.03.5

Abstract

Water pollution can make many problems such as, the incidence of disease and poisoning. Pollution in water can produce free radicals and it is can trigger of disease for aquatic organisms. There is some efforts that can be done to provide this problem, such as chemical compound that can reduce the reaction of free radicals. Antioxidants are one of the chemical compounds that can reduce the activity of free radicals. Eucheuma cottonii is the one of a seaweed that has many in antioxidant compounds, such as phenol compounds, but it is also rich in iodine fiber and other important minerals. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of seaweed extraction. Identification of Eucheuma cottonii extract is using FTIR test. The last stage is an antioxidant activity test that includes DPPH test (1,1-diphenyl-2-picrilhidrazil) and Inhibition Concentration 50 (IC 50). The results obtained in this study were based on FTIR test of antioxidant compound in Eucheuma cottonii seaweed extract. The one of compounds that have antioxidant activity include galaktosa-4-sulfat. Based on the results of antioxidant activity test using DPPH obtained that seaweed extract Eucheuma cottonii active as an antioxidant to ward off free radicals in the waters. The concentration of Eucheuma cottonii seaweed extract for preventive 50% concentration of DPPH is 39,926 ppm.
ANALISIS KUALITAS LINGKUNGAN SUNGAI WANGI-BEJI, PASURUAN YANG DIDUGA TERCEMARI OLEH LIMBAH PABRIK, PEMUKIMAN DAN PERTANIAN Adam, Moh. Awaludin; Maftuch, Maftuch; Kilawati, Yuni; Risjani, Yenny
Samakia : Jurnal Ilmu Perikanan Vol 8 No 2 (2017): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (544.375 KB) | DOI: 10.5281/jsapi.v8i2.263

Abstract

Penelitian ini bertujuan untuk mengetahui kualitas lingkungan, kadar dan sebaran logam berat di perairan aliran Sungai Wangi, Kecamatan Beji, Kabupaten Pasuruan dalam kaitannya untuk kepentingan pelestarian lingkungan sekitar aliran sungai tersebut. Metode pengambilan sampel dilakukan dengan secara terpilih, dengan memperhatikan kondisi serta keadaan dari daerah penelitian, arus dan kedalaman daerah penelitian. Hal ini dimaksudkan untuk melihat sampai  sejauh mana konsentrasi zat pencemar menyebar. Pengamatan kualitas air selama penelitian pada musim yang berbeda yaitu musim hujan (bulan Oktober 2016 sampai Februari 2017) dan musim kemarau (bulan Maret sampai September 2017). Berdasarkan hasil pengujian kualitas lingkungan dan logam berat dapat disimpulkan bahwa kualitas lingkungan pada aliran sungai Wangi sudah berada pada ambang batas, sehingga diperlukan kegiatan penanggulangan limbah dan pelestarian lingkungan.Hal ini harus mendapatkan perhatian yang besar dari masyarakat secara umum dan pemerintah daerah secara khusus.Harapan ke depannya adalah pencemaran sungai Wangi dapat dihindari dan ditanggulangi.
Phytogenetic Relationships of Mystacoleucus marginatus (Valenciennes 1842) based on Cytochrome Oxidase C Subunit I (COI) Gene Valen, Fitri Sil; Widodo, Maheno Sri; Kilawati, Yuni; Islamy, R. Adharyan
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.3

Abstract

Mystacoleucus marginatus is a type of freshwater fish that is spread in various countries with tropical climates, one of which is Indonesia. This fish can be found in the Brantas River Basin (DAS), East Java. In its development, identification of fish species needs to be done in detail and more deeply, so that the correct identification results can be obtained. This study aims to identify genetic and phylogenetic characteristics of Mystacoleucus marginatus in the Brantas River Basin based on the Cytochrome Oxidase C Subunit I (COI) gene. Test samples were taken from 11 individuals of Mystacoleucus marginatus in the Brantas watershed, from June to August 2018. The results showed that Mystacoleucus marginatus in the Brantas watershed had 2 Haplotypes with Haplotype (Hd)=0.96 diversity values and nucleotides diversity (Pi)=0.00357. These results indicate that the population in the Brantas watershed is interconnected between one location and another sampling location, this is supported by a phylogenetic topology of DNA barcodes showing a monophyletic clade, as well as a haplotype distribution indicating that the sample area is genetics are not isolated from one another. So that the type of Mystacoleucus marginatus scattered along the Brantas watershed is still in one species, which is indicated by the low level of genetic variation. Genetic identification efforts must be carried out continuously so that appropriate information can be obtained for the diversity of fish species, especially endemic fish.
ANALISIS KUALITAS LINGKUNGAN SUNGAI WANGI-BEJI, PASURUAN YANG DIDUGA TERCEMARI OLEH LIMBAH PABRIK, PEMUKIMAN DAN PERTANIAN Adam, Moh. Awaludin; Maftuch, Maftuch; Kilawati, Yuni; Risjani, Yenny
Samakia : Jurnal Ilmu Perikanan Vol 9 No 1 (2018): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.393 KB) | DOI: 10.5281/jsapi.v9i1.217

Abstract

Penelitian ini bertujuan untuk mengetahui kualitas lingkungan, kadar dan sebaran logam berat di perairan aliran Sungai Wangi, Kecamatan Beji, Kabupaten Pasuruan dalam kaitannya untuk kepentingan pelestarian lingkungan sekitar aliran sungai tersebut. Metode pengambilan sampel dilakukan dengan secara terpilih, dengan memperhatikan kondisi serta keadaan dari daerah penelitian, arus dan kedalaman daerah penelitian. Hal ini dimaksudkan untuk melihat sampai  sejauh mana konsentrasi zat pencemar menyebar. Pengamatan kualitas air selama penelitian pada musim yang berbeda yaitu musim hujan (bulan Oktober 2016 sampai Februari 2017) dan musim kemarau (bulan Maret sampai September 2017). Berdasarkan hasil pengujian kualitas lingkungan dan logam berat dapat disimpulkan bahwa kualitas lingkungan pada aliran sungai Wangi sudah berada pada ambang batas, sehingga diperlukan kegiatan penanggulangan limbah dan pelestarian lingkungan.Hal ini harus mendapatkan perhatian yang besar dari masyarakat secara umum dan pemerintah daerah secara khusus.Harapan ke depannya adalah pencemaran sungai Wangi dapat dihindari dan ditanggulangi.