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Ethnomycology of Bracket Fungi in Baduy Tribe Indonesia Khastini, Rida Oktorida; Wahyuni, Indria; Saraswati, Irma
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14082

Abstract

Inhabitants of the Kendeng mountain region depend on the biodiversity of the forest resources for their livelihood. Mushrooms are important resource that provide benefits to mankind. The aim on this research was to document traditional knowledge in bracket fungi utilization to treat health problems in Baduy tribe community. The results described in this paper were obtained through the exploration and identification of bracket fungi as well as the interview to determine their cultural significance for Baduy people. Quantitative approaches were used to determine the Use Values (UV), Informant Consensus Factor (ICF) and Fidelity level (FL) values. A total of 6 species that were identified as bracket fungi were included to Basidiomycota. The Baduy community considers the main use of bracket fungi as a medicine for several ailments. The highest UV, ICF and FL value were obtained by Ganoderma lucidum. This study provides the information regarding bracket fungi species with the high use values that could be employed in pharmacological research and further biotechnological approaches in order to achieve an adequate revenue.
Production and Regeneration Protoplast of Dark-Septate Endophytic Fungus Veronaeopsis simplex Khastini, Rida Oktorida
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Abstract

The hyphomycete, Veronaeopsis simplex, belongs to the group of so-called dark septate endophytes (DSE) colonize root plant intra and intercellulary without causing harm for the plant host. Knowledge about fungus interaction with the host plant is little. This paper describes the influence of different cultural conditions on protoplast isolation and regeneration from V. simplex fungus. As protoplast is interesting structure that can be used on developing an engineered DSE fungus by mutagenesis or genetic transformation, this research will provide the foundation for further study in order to reveal the unknown mechanism of the fungal role in the plant host. The mycelia age, osmotic stabilizer and lytic enzyme were the main factor that affected the protoplast formation.  The maximum yield of protoplast was 3.2 x 106/g fresh mycelia was obtained from 1 week old mycelia digested with the mixed of 1.5% cellulose, 1 % macerozyme and 1.5 % lysozyme for 3 h incubation at 37oC in the dark. Morphology and structure of protoplast released were observed using microscope. It was found that the regeneration of protoplast was possible after being cultivated on both CMMY and PDA medium supplemented with 0.6 M sucrose.Keywords: DSE, isolation, regeneration, protoplast, Veronaeopsis simplex 
Production and Regeneration Protoplast of Dark-Septate Endophytic Fungus Veronaeopsis simplex Khastini, Rida Oktorida
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Abstract

The hyphomycete, Veronaeopsis simplex, belongs to the group of so-called dark septate endophytes (DSE) colonize root plant intra and intercellulary without causing harm for the plant host. Little is known about fungus interaction with the host plant. This paper describes the influence of different cultural conditions on protoplast isolation and regeneration from V. simplex fungus. As protoplast is interesting structure that can be used on developing an engineered DSE fungus by mutagenesis or genetic transformation, this research will provide the foundation for further study in order to reveal the unknown mechanism of the fungal role in the plant host. The mycelia age, osmotic stabilizer and lytic enzyme were the main factor that affected the protoplast formation.  The maximum yield of protoplast was 3.2 x 106/g fresh mycelia was obtained from 1 week old mycelia digested with the mixed of 1.5% cellulose, 1 % macerozyme and 1.5 % lysozyme for 3 h incubation at 37oC in the dark. Morphology and structure of protoplast released were observed using microscope. It was found that the regeneration of protoplast was possible after being cultivated on both CMMY and PDA medium supplemented with 0.6 M sucrose. Keywords: DSE, isolation, regeneration, protoplast, Veronaeopsis simplex
PENGARUH PENERAPAN STRATEGI BELAJAR PQ4R (PREVIEW, QUESTION, READ, REFLECT, RECITE, REVIEW) MENGGUNAKAN MEDIA KOMIK PADA KEMAMPUAN KOGNITIF SISWA PADA MATERI SISTEM SARAF Agustina, Lia; Khastini, Rida Oktorida; Fitri, Siti Gia S.
BIODIDAKTIKA, JURNAL BIOLOGI DAN PEMBELAJARANNYA Vol 10, No 2 (2015)
Publisher : JURNAL BIOLOGI DAN PEMBELAJARANNYA

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Abstract

This research aimed to determine the effect of PQ4R learning strategy using comic media on students interest and cognitive ability toward the nervous system concept. The method was a quasi-experimental desain with randomized control group pretest posttest. PQ4R learning strategy using comic media was as independent variables, and cognitive abilities was as dependent variables. Population in this research were all of students classXIIPA1Ciomas High School, academic year 2012-2013. Overall subject of population was used as the sample of research.Sample class XI IPA 1 as an experiment class and XI IPA 2 as a control class. Sampling technique used random sampling. Instrument used were multiple choice test for determining students cognitive ability, observation sheets and documentation. All the instrument, were analyzed by t testfor hypothesis.The resultof hypothesis showed the influence of PQ4R learning strategyusingcomic media on students interest, cognitive ability
PROFIL KETERAMPILAN PSIKOMOTOR MAHASISWA CALON GURU BIOLOGI MELALUI PRAKTIKUM KULTIVASI MIKROORGANISME Khastini, Rida Oktorida; Sapriati, Amalia; Sekarwinahyu, Mestika
BIODIDAKTIKA, JURNAL BIOLOGI DAN PEMBELAJARANNYA Vol 12, No 1 (2017)
Publisher : JURNAL BIOLOGI DAN PEMBELAJARANNYA

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Abstract

AbstractGeneral microbiology course provide comprehensive introduction to fundamental aspects of microbial world so that student gain an insight into theory, practices and able to demonstrate in the term of knowledge skills and values to overcome problem in the environment. Therefore student should have psychomotor skill as well as cognitive. The present study aimed to identify and describe the psychomotor skills of Pre Service Biology Teacher through microorganism cultivation experiment in the laboratoratory. Data were collected from 94 Pre Service Biology Teacher who enrolled General microbiology course in the even semester of academic year 2016-2017 using psychomotor skill observation sheet. Six dimensions observed: perception, set, guided response, mechanism, adaptation and originalation. Findings indicated that student psychomotor skills was categorized as good with the value of 81,2%.
LEARNING PROCESS OF BIOLOGY THROUGH THE GUIDED DISCOVERY LEARNING BASED ON LOCAL EXCELLENCE ON THE SUBCONCEPT OF EUBACTERIA ROLE IN LIFE SURATMI, SURATMI; KHASTINI, RIDA OKTORIDA; FAUZIYAH, NUR LAILI
Sriwijaya University Learning and Education International Conference Vol 2, No 1 (2016): 2nd SULE-IC
Publisher : Sriwijaya University Learning and Education International Conference

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Abstract

The purposes of this study were to determine learning process of biology through the guided discovery learning based local excellence on subconcepts eubacteria role in life and to determine the skills of the students process through the guided discovery learning based local excellence. The method used was a descriptive method. The population in this research was class of X of SMAN 13 Tangerang District. The sampling technique used in this study was purposive sampling. The sample was the class X.A of SMAN 13 Tangerang District consisted of 46 students. The data were collected by observations, questionnaires and skills process of science test. The results of this study showed that student responses during learning activities using guided discovery learning based local excellence on subconcepts eubacteria role in life was good with a percentage of 77.34%. The skills process of the students belong to the good categories with an average percentage of 82.52%.
Developing Creativity and Entrepreneurial Values in High School Student Through Project Based Learning Model On Biotechnology Concept Ulfah, Yayu Maria; Khastini, Rida Oktorida; Djazimi, Ika Rifqiawati; Mulhayati, Fazkhur Fajar; Sukanan, Wuni Wandira; Sapriati, Amalia
Jurnal Pendidikan IPA Indonesia 2018: Article in Press
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v0i0.10524

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penerapan model pembelajaran berbasis proyek berorientasi bioentrepreneurship terhadap kreativitas dan nilai-nilai kewirausahaan siswa kelas XII SMA pada sub topik bioteknologi konvensional. Penelitian ini menggunakan metode kuasi eksperimen dengan desain penelitian The posttest only control group design. Sampel penelitian yaitu kelas XII IPA 2 sebagai kelas eksperimen dan kelas XII IPA 1 sebagai kelas kontrol yang dipilih secara random sampling. Pengumpulan data menggunakan instrumen tes uraian kemampuan berpikir kreatif, rubrik penilaian produk kreatif, lembar observasi dan angket nilai-nilai kewirausahaan. Hasil analisis hipotesis menggunakan uji-t didapatkan signifikansi kreativitas dari produk kreatif sebesar 0,000 < 0,05 dan dari kemampuan berpikir kreatif sebesar 0,001 < 0,05, sehingga dapat disimpulkan bahwa model pembelajaran berbasis proyek berorientasi bioentrepreneurship berpengaruh terhadap kreativitas siswa. Signifikansi nilai-nilai kewirausahaan dari lembar observasi sebesar 0,000 < 0,05 dan dari angket sebesar 0,019 < 0,05, sehingga dapat disimpulkan bahwa model pembelajaran berbasis proyek berorientasi bioentrepreneurship berpengaruh pada nilai-nilai kewirausahaan. Hasil analisis korelasi disimpulkan bahwa terdapat hubungan yang sangat rendah diantara keduanya, dengan nilai koefisien korelasi sebesar 0,195. Hasil uji regresi linier menunjukan bahwa kreativitas memberikan kontribusi sebesar 3% terhadap nilai-nilai kewirausahan siswa.ABSTRACTEntrepreneurship have long been associated with creativity and need to be developed among students. A quasi experimental method with the posttest only control group design was conducted to determining the influence of project-based learning model oriented to bioentrepreneurship for XII grade students creativity and entrepreneurial values on conventional biotechnology sub topic. The sample was XII science 2 as experimental class and XII science 1 as control class chosen by random sampling. The data collected by using creative thinking ability test, creative product valuation rubric, observation sheet and entrepreneurial values questionnaire. The hypothesis analyzed by using t-test which obtained the significance of creative products was 0,000 < 0,05 and the creative thinking ability was 0,001 < 0,05, so it can be concluded that project-based learning model oriented to bio entrepreneurship effected on student creativity. As for the significance of entrepreneurial values from the observation sheet was 0,000 < 0,05 and the questionnaire was 0,019 < 0,05, so it would be concluded that project-based learning model with bioentrepreneurship oriented effected on students entrepreneurial values. Correlation analysis concluded that there was a correlation between them, with the value of correlation coefficient was 0,195. The result of linear regression test showed that creativity contributed about 3% to students entrepreneurial values.
INVENTORY AND UTILIZATION OF MACROFUNGI SPECIES FOR FOOD IN CIKARTAWANA INNER BADUY BANTEN Khastini, Rida Oktorida; Wahyuni, Indria; Lista, Lista; Saraswati, Irma
BIODIDAKTIKA, JURNAL BIOLOGI DAN PEMBELAJARANNYA Vol 14, No 1 (2019): Biodidaktika: Jurnal Biologi dan Pembelajarannya
Publisher : JURNAL BIOLOGI DAN PEMBELAJARANNYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/biodidaktika.v14i1.4838

Abstract

This research aimed to conduct an inventory in macrofungi and the utilization for food in Cikartawana hamlet, Inner Baduy, Banten. The method that was used in this research was the explorative method with descriptive analysis. The result of this research showed a total of 52 species of Macrofungi that belonged to 23 families have been identified and among those species about 12 species were edible and consumed by Inner Baduy people in Cikartawana hamlet. The macrofungi grew in the different type of substrate: soils, on decaying organic matter, wooden stumps and living tree trunk collected from different habitat from the surrounding environment. Inner Baduy people used edible macrofungi either fresh or dried in elaborated stew such as soup, steamed or stir-fried. This study provides information regarding the number of macrofungi species for harnessing its potential and conserve the biodiversity and ecosystems.
ISOLASI, DAN PENAPISAN CENDAWAN ENDOFIT AKAR ASAL RHIZOSFER TALAS BENENG Khastini, Rida Oktorida
Jurnal Biotek Vol 6, No 2 (2018): Desember
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v6i2.6823

Abstract

Kebutuhan pangan nasional dapat dilakukan upaya diversifikasi pangan yaitu pemanfaatan kelompok umbi-umbian seperti Talas Beneng (Xanthosoma undipes K.Koch) yang merupakan salah satu jenis flora umbi-umbian khas Provinsi Banten. Produktivitas talas beneng dapat ditingkatkan yaitu salah satunya dengan menggunakan pupuk hayati mengandung mikroorganisme hidup yang bermanfaat. Penelitian ini bertujuan untuk mengisolasi dan melakukan penapisan terhadap cendawan endofit akar asal rhizosfer talas beneng sehingga dapat dikembangkan lebih lanjut sebagai pupuk hayati untuk meningkatkan produktivitas talas beneng. Sebanyak 19 isolat cendawan berhasil diisolasi melalui teknik isolasi langsung dan pengumpanan. Hasil identifikasi menunjukkan jenis cendawan dari genus Acremonium, Aspergillus, Aureobasidium, Curvularia, Fusarium, Penicillium, Paecilomyces, Trichoderma, dan beberapa isolat yang belum teridentifikasi. Berdasarkan hasil penapisan diperoleh sebanyak 12 isolat merupakan cendawan endofit akar dan 5 isolat merupakan cendawan parasit akar. Kata Kunci: Cendawan endofit akar, talas beneng, rhizosfer
Ragam Liken Berdasarkan Ketinggian Dataran sebagai Bioindikator Kualitas Ekosistem di Cagar Alam Rawa Danau Serang Banten Khastini, Rida Oktorida; Juwitasari, Indah; Sulasanah, Siti
Biota Vol 11 No 2 (2018)
Publisher : UIN Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1384.472 KB) | DOI: 10.20414/jb.v11i2.143

Abstract

Liken merupakan suatu organisme hasil dari simbiosis&nbsp; alga dan fungi yang saling menguntungkan. Organisme ini dapat dijadikan sebagai indikator lingkungan untuk memantau kelestarian alam terutama wilayah yang dilindungi. Tujuan dari penelitian ini adalah untuk mengetahui ragam liken sebagai bioindikator kualitas lingkungan CA. Rawa Danau Eksplorasi liken dilakukan pada empat stasiun berdasarkan ketinggian dataran, yaitu 90 mdpl, 100-130 mdpl, 130-160 mdpl, 160-187 mdpl. Berdasarkan hasil penelitian diperoleh liken sebanyak 63 spesimen yang terdiri dari 19 spesies, 17 genus, dan 13 famili. Keanekaragaman liken paling tinggi yaitu pada stasiun 1 (90 mdpl), sedangkan terendah terdapat pada stasiun 2 (100-130 mdpl). Indeks keanekaragaman liken dari yang terendah hingga tertinggi mencapai H’ 1.494-H’ 2.667 yang berarti keanekaragaman liken yang berada di Cagar Alam Rawa Danau tergolong keanekaragaman liken sangat tinggi. Kondisi lingkungan di Cagar Alam Rawa Danau terkini dapat dilihat dari respon liken sebagai bioindikator berdasarkan indeks kriteria kualitas ekosistem berdasarkan Indeks Shannon-Wienner bahwa kondisi lingkungan di Cagar Alam Rawa Danau termasuk kedalam kriteria kualitas ekosistem sangat baik &nbsp;