Fery Abdul Choliq
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Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang-Hai; Sulistyowati, Liliek
The Journal of Experimental Life Science Vol 7, No 1 (2017)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1118.483 KB) | DOI: 10.21776/ub.jels.2016.007.01.01

Abstract

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of 19.5 kDa as the size of coat protein of tobamoviruses was estimated by sodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigid rod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp. in Taiwan.Keywords: Frangipani plant, FrMV, mosaic disease, Tobamovirus.
Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (18.26 KB)

Abstract

Abstract Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan. Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus
Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan. Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus
Distribution of the Endophytic Fungi in Apple Leaves Afandhi, Aminudin; Choliq, Fery Abdul; Anggrilika W.S., Havinda; Tarno, Hagus
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.1563

Abstract

Endophytic fungi have roles to protect the plant from herbivorous insects and diseases, and to support the absorption process of nutrients needed by plants for photosynthesis. Related to the important role of endophytic fungi, research was aimed to identify fungal endophytes associated with young, mature and old leaves on apple and to evaluate the effect of leaf ages on the abundance and diversity of endophytic fungi. The research was conducted in Biological Control Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya from May to September 2016. Apple leaves sampling was done diagonally, and taken from nine apple trees. Based on macroscopic and microscopic characteristics, 38 isolates were identified. In the old apple leaves, it obtained 17 isolates that consisted of 5 genera, mature leaves obtained 14 isolates consisted of 6 genera, and young leaves 7 isolates obtained consists of 2 genera. The mature leaves have highest abundance and diversity of endophytic fungi compared to young and old leaves. Endophytic fungi apple leaf dominated by Aspergillus. Mature and young leaves were different from old one based on Bray-Curtis similarity.
Identifikasi Penyakit yang Disebabkan oleh Virus pada Tanaman Pepaya (Carica papaya L.) di Malang, Jawa Timur Choliq, Fery Abdul; Astono, Tutung Hadi; Istiqomah, Istiqomah; Fauziyah, Miladiyatul
Gontor AGROTECH Science Journal Vol 4, No 2 (2018): December 2018
Publisher : University of Darussalam Gontor, Ponorogo, East Java Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/agrotech.v4i2.2442

Abstract

Abstract : Papaya ringspot merupakan penyakit baru pada tanaman pepaya di Indonesia yang disebabkan  oleh patogen Papaya ringspot virus (PRSV). Tujuan dari penelitian ini yaitu untuk mengetahui virus penyebab penyakit pada tanaman pepaya di Malang. Penelitian ini dilaksanakan di Laboratorium Virologi, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya dan rumah kaca di Desa Karangwidoro, Kecamatan Dau, Kabupaten Malang. Metode penelitian yang digunakan yaitu metode deskriptif  untuk mengetahui jenis virus pada tanaman pepaya. Hasil penelitian menunjukkan gejala pada tanaman pepaya di lapang yaitu mosaik dan klorosis pada lamina daun serta adanya bercak cincin atau ringspot pada permukaan buah. Hasil penularan pada tanaman indikator Chenopodium amaranticolor dan Chenopodium quinoa menghasilkan gejala lesio lokal nekrosis. Berdasarkan hasil identifikasi menggunakan TEM morfologi partikel virus tergolong kelompok Potyvirus, berbentuk filament flexuous dengan ukuran sekitar 800-900 nm x 12 nm. Virus yang diuji mempunyai kisaran inang tanaman dari famili Caricaceae dan dua jenis tanaman dari famili Cucurbitaceae yaitu Cucumis sativus dan Cucumis melo L. Berdasarkan hasil pengujian sifat fisik virus diketahui nilai Dilution End-Point (DEP), Thermal Inactivation Point (TIP), dan Longevity In Vitro (LIV) virus yang diuji yaitu 10-5-10-6, 65oC, dan 24-48 jam pada suhu ruang. Berdasarkan nilai tersebut dapat disimpulkan virus yang menginfeksi tanaman pepaya di Malang termasuk dalam famili Potyvirus yaitu Papaya ringspot virus (PRSV).