Bagus Herwibawa
University of Jenderal Soedirman

Published : 6 Documents
Articles

Found 6 Documents
Search

PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice’s coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and non-aromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (IU-2, IU-3, IU-4, IU-5, IU-6, IU-7, and IU-8), but there were some genotypes to able to improve the resistance under drought stress (R-2, R-3, R-5, R-6, R-7, IT-4, IT-5, and IT-7). Keywords:  drought resistance, gamma irradiation, peroxidase isozyme, rice, sodium azide
APLIKASI TEKNOLOGI BIOSLURRY DI DESA MONTONGSARI KABUPATEN KENDAL PROVINSI JAWA TENGAH Kusmiyati, Florentina; Herwibawa, Bagus; Budiyanto, Susilo
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 6, No 2 (2017): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (721.356 KB) | DOI: 10.33230/JLSO.6.2.2017.315

Abstract

Kusmiyati et al, 2017. Application of Bioslurry Technology at Montongsari Village, Kendal Regency Central Java Province. JLSO 6(2):106-112.The dependence of farmers on inorganic fertilizers is increasing from year to year. The urea requirement for agriculture in 2015 was 3.795.596 tons, increased to 4.007.463 tons in 2016. Many efforts have been made to reduce farmer?s dependence on inorganic fertilizers. The activity purpose of Student Community Service-Community Empowerment Learning was to reduce farmer?s dependence on inorganic fertilizers by using bioslurry at Montongsari village, Kendal regency, Central Java. The activities were training, mentoring and demonstration plot. Training and mentoring activities were  processing of biogas wastes into solid and liquid bioslury fertilizers and their application on plant. The treatments of demonstration plot were without and with liquid bioslurry on growth and production of water spinach (Ipomoea aquatica). The result of the mentoring activities showed a biogas reactor (capacity of 4 m3) will produce 18 kg of bioslury from 20-40 kg of goat dung. Nitrogen content (N), C-organic  and C / N ratio of solid bioslury were 1.43%; 37.61% and 26.30, respectively. While nitrogen content of liquid bioslury was only 0.09%.  There were no growth difference between aplication of liquid bioslurry and inorganic fertilizer on water spinach. The conclusion was  bioslury of biogas waste both solid and liquid bioslury can be utilized as organic fertilizer for plants.
MUTAGENIC EFFECTS OF SODIUM AZIDE ON THE GERMINATION IN RICE (Oryza sativa L. cv. INPAGO UNSOED 1) Herwibawa, Bagus; Kusmiyati, Florentina
JURNAL AGROTEKNOLOGI Vol 7, No 2 (2017): Februari 2017
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The available natural diversity highly limits the effort to improve the production of upland-rice with delicious and aromatic flavors. However, the mutagenesis can help improving the natural diversity. The aromatic-upland rice seeds, cv. Inpago Unsoed 1, were soaked in solution of NaN3 with the doses of 0 mM, 1 mM, 2 mM, 3 mM, 4 mM, 5 mM, 6 mM, 7 mM, 8 mM, 9 mM, and 10 mM, in four replications in completely randomized design. The observation included LD50, germination percentage at first-count and final-count. Data were tabulated and analyzed with CurveExpert 1.4 software for LD50, and generalized linear model in the PROC-GLM procedure of SAS 9.1 software. The means were generated and compared through Dunnett option, at probability level of 5%, in order to determine the difference in means between mutated and non-mutated seeds. The results show that the attributes of rice physiology affected by mutagenesis are sensitivity of rice to NaN3 with LD50 at 8.84 mM, and significant effect of NaN3 on the decreasing capacity of seed germination at > 6 mM for first count, and > 7 mM for final count.Keywords: germination, inpago unsoed 1, median lethal dose, rice, sodium azide  
PENDAMPINGAN PEMANFAATAN PUPUK ORGANIK BIOSLURRY UNTUK RUMAH PANGAN LESTARI DI DESA MONTONGSARI KABUPATEN KENDAL Kusmiyati, Florentina; Budiyanto, Susilo; Herwibawa, Bagus
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 24, No 1 (2018): JANUARI - MARET
Publisher : LPM Unimed

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AbstrakKetahanan pangan mensyaratkan kondisi tercukupinya pangan bagi seluruh elemen masyarakat, berkualitas, aman, beragam, bergizi, merata, serta mudah dijangkau. Rumah tangga memiliki peran penting sebagai satu diantara banyak faktor pendukung terwujudnya ketahanan pangan nasional, misalnya dengan pembentukan Rumah Pangan Lestari (RPL) yang berwawasan pertanian organik.Selain memiliki nilai ekonomi, kesehatan, dan ekologi, pembentukan RPL juga akan mengoptimalkan pemanfaatan pekarangan dan ruang-ruang sempit di sekitar rumah. Artikel ini merupakan rangkuman dari satu diantara beberapa kegiatan Kuliah Kerja Nyata - Pembelajaran Pemberdayaan Masyarakat(KKN-PPM UNDIP) di desa Montongsari, kabupaten Kendal. Kegiatan ini bertujuan untuk memberdayakan kelompok ibu-ibu rumah tangga yang tidak bekerja, untuk memanfaatkan limbah biogas atau biosurry sebagai pupuk organik dalam budidaya tanaman dengan teknik vertikultur di sekitar rumah. Metode yang digunakan adalah survei, penyuluhan, pelatihan, dan pendampingan pembuatan RPL dengan memanfaatkan bioslurry. Hasil kegiatan ini memberikan dampak positif bagi masyarakat, yaitu mulai timbul kesadaran dan perubahan perilaku masyarakat. Bioslurry yang sebelumnya belum dimanfaatkan, dapat diaplikasikan sebagai pupuk organik. Lahan kosong di sekitar rumah dan sepanjang kiri-kanan jalan yang selama ini tidak dimanfaatkan, mulai dimanfaatkan sebagai kawasan yang menunjang pangan masyarakat. Lingkungan yang dulu terkesan gersang berubah menjadilebih asri. Oleh sebab itu untuk mendukung keberlanjutan RPL, diperlukan keinginan yang serius dan tindakan nyata dari pemerintah dan masyarakat.Kata kunci: Bioslurry, Kelestarian Lingkungan, Organik, Pangan, Rumah Pangan LestariAbstractFood security exist when all people at all times have access to sufficient, safe, and nutritious food. Households have an important role in national food security, by creating the Sustainable Food Reserve Gardens based on organic principles. It has economic, health, and ecological benefits. Sustainable Food Reserve Gardens also will optimize the spaces arround the houses. This article is a summary of one of the Student Community Service - Community Empowerment Learning of Diponegoro University activities in Montongsari village, Kendal Regency. This program aims was to empower the group of housewives to use biogas residue or bioslurry as organic fertilizer in verticulture arround the houses. The methods used were survey, extension, training, and outreach in application of bioslurry organic fertilizer for Sustainable Food Reserve Gardens. This program had positive impact for the community, including the emergence of awarenees and behavior changes of community. Bioslurry can be appliedas organic fertilizer. Spaces arround the houses can provide accessibility of fresh food for community. The village seems to change to become more beautiful. Therefore, to support the Sustainable Food Reserve Gardens, it requires serious commitment and real action of the government and community. Keywords: Bioslurry, Environmental Sustainability, Organic, Food, Sustainable Food Reserve Gardens
PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v36i3.436

Abstract

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice?s coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and non-aromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (IU-2, IU-3, IU-4, IU-5, IU-6, IU-7, and IU-8), but there were some genotypes to able to improve the resistance under drought stress (R-2, R-3, R-5, R-6, R-7, IT-4, IT-5, and IT-7). Keywords:  drought resistance, gamma irradiation, peroxidase isozyme, rice, sodium azide
THE EFFECT OF GAMMA IRRADIATION AND SODIUM AZIDE ON GERMINATION OF SOME RICE CULTIVARS Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 1 (2014)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v36i1.360

Abstract

Efforts to increase rice production through genetic improvement are often limited by the availability of natural diversity. That natural diversity can be improved through induced mutation. Selected characters can be observed since the germination phases, which may also indicate the plants survival under field conditions. Experimental design was arranged in split plot, with cultivars as main plot and mutagen doses as sub plot. The experiment consisted of 36 treatment combinations, with each treatment consisting of 10 replications. Data were recorded on percentage of first count, final count and germination rate. The data were analyzed using F-test by SAS 9.0 and mean separation was carried out by employing DMRT at 95 % (? = 5 %) of confidence level. The results showed that the best cultivar response for germination traits was Inpari 13, the best mutagen to build cultivar for germination traits was Gamma 150 Gy, and the best combination between cultivar and mutagen for germination traits was Inpago Unsoed 1 that was treated with Gamma 150 Gy. Keywords: gamma irradiation, germination, rice, sodium azide