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Uji Hemaglutinasi Assay pada Hemolimf Spodoptera exigua yang diberi Perlakuan Botani Pestisida Suryani, A.Irma; Anggraeni, Tjandra
bionature Vol 20, No 1 (2019): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.943 KB) | DOI: 10.35580/bionature.v20i1.9758

Abstract

Abstract. The larval stage of the armyworm (Spodoptera exigua), has a high and polyphagous feeding activity so that these insects have the potential to be pests in agriculture. The use of botanical pesticides in this case Mirabilis jalapa, is expected to control agricultural pests so that research on the immune system of S. exigua larvae is carried out by observing lectins that play a role in analyzing foreign molecules that enter the insect's body. The results were obtained, the higher the M. jalapa concentration, at the concentration of M. jalapa 0.4% and 0.8%, the lower the hemolymf agglutination titers. This is thought to decrease the binding capacity of lectins to foreign molecules. So that M. jalapa has the potential to be used as an environmentally friendly botanical pesticide.Keywords: agricultural pests, botanical pesticides, lectin agglutination.
Promoting Dolichoderus thoracicus as an Agent to Disperse Trichoderma sp., a Fungus that Controls the Black Pod Disease, Central Sulawesi, Indonesia Anggraeni, Tjandra; Umrah, U.; Esyanti, Rizkita R.; Aryantha, I Nyoman P.
Journal of Mathematical and Fundamental Sciences Vol 46, No 1 (2014)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.186 KB) | DOI: 10.5614/j.math.fund.sci.2014.46.1.4

Abstract

In this study, we propose to use Dolichoderus thoracicus to act as a double agent; not only as an agent to control cocoa plant pests, but also as an agent for distributing Trichoderma sp., a fungus that suppresses the development of the black pod disease caused by Phytophthora palmivora. In the experiments, D. thoracicus was more attracted to honey-soil media (M5) and coconut waste pulp-soil media (M6). However, 10% sucrose-potato-soil media (M3) was the best media for growing Trichoderma sp. Morphological study showed that spores attached to many parts of D. thoracicus. The efficiency of D. thoracicus in distributing the spores compared to the conventional method of using a sprayer was measured. The results showed that the growth of P. palmivora was suppressed by 83.33%, which is not significantly different from when Trichoderma was dispersed through spraying (87% suppressed). It was concluded that D. thoracicus can act as a double agent and can be used to disperse Trichoderma sp.
Banana Flower-Insect Interaction: Alpha-Pinene as Potential Attractant for the Insect Vector of Banana Blood Disease Masriany, Masriany; Esyanti, Rizkita R.; Dwivany, Fenny M.; Anggraeni, Tjandra
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.922 KB) | DOI: 10.4308/hjb.27.1.8

Abstract

Volatile metabolites are produced by plants for self-defense and as communication mediators with the environment. Terpenes are volatiles emitted as odorant cues for herbivores and microorganisms. This study was aimed to investigate volatile metabolites produced by banana flowers that attract insect vectors of BBD. The volatile metabolites from banana flowers were extracted by headspace-solid phase microextraction (HS-SPME) and identified by gas chromatography–mass spectrometry (GC-MS). It was apparent that the concentrations of the metabolite alpha-pinene gradually increased from the first to the the third stage. Comparison of metabolites produced by symptomatic banana male flowers for BBD infection with non-symptomatic ones showed that the concentration of alpha-pinene was higher in symptomatic male flowers. In addition, preference for alpha-pinene was tested on three insect vector species (Rhodesiella bhutanensis, Drosophila sp., and Musca sp.), analyzed by M. Anova p<0.001, F(1.5) =12.539 and Duncan test. Results showed that the insect vectors were mostly attracted to 20 µl volume of alpha-pinene compared to the other volumes and that alpha-pinene functioned as an attractant to these insects. This research is important for the formulation of attractants for insect vectors of BBD to control transmission of banana blood disease.
PENGARUH EKSTRAK MIRABILIS JALAPA TERHADAP JUMLAH HEMOSIT LARVA SPODOPTERA EXIGUA Suryani, A. Irma; Anggraeni, Tjandra
Chemica: Jurnal Ilmiah Kimia dan Pendidikan Kimia Vol 16, No 2 (2015)
Publisher : Jurusan Kimia FMIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.138 KB) | DOI: 10.35580/chemica.v16i2.4557

Abstract

ABSTRAKEkstrak tanaman Mirabilis jalapa diketahui berperan sebagai biopestisida alami. Larva Spodoptera exigua merupakan salah satu hama dalam bidang pertanian jika jumlahnya berlebih di alam.  Konsentrasi subletal tanaman M. jalapa 0.4% dan 0.8% yang diberikan pada pakan larva S. exigua, akan mempengaruhi sistem imun larva berupa penurunan  jumlah total konsentrasi hemosit sebesar  1.66 ± 0.47  dan 1.01 ± 0.07 (sel/mL x 106). Kata kunci: Mirabilis jalapa, Spodoptera exigua, hemosit, system imun ABSTRACTExtracts of Mirabilis jalapa plant, known to act as a natural biopesticide Spodoptera exigua larvae is one of the pests in agriculture if the amount is excessive in nature. M. jalapa plant sublethal concentrations of 0,4 % and 0,8 % were given to the larvae of a decrease in the total number of hemocytes concentration of 1.66 ± 0.47 and 1.01 ± 0.07 (cell/mL x 106). Keywords: Mirabilis jalapa, Spodoptera exigua, hemocytes, immune system
Infeksi Subletal Bacillus thuringiensis pada Helicoverpa armigera: Mungkinkah Menyebabkan Resurgensi? Anggraeni, Tjandra; Putra, Ramadhani Eka; Ahmad, Intan
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 1 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9946.571 KB) | DOI: 10.22146/jpti.10016

Abstract

Research on the effect of B. thuringiensis sublethal infection to the chance of H. armigera to resurgence has been conducted. Third instar insects were used and the concentration applied were 0 ppm, 150 ppm, 300 ppm, 450 ppm and 600 ppm. The result showed that along with the increase of B. thuringiensis infection, the number and the viability of the eggs production declined. In addition, the weight difference between the 3rd and the 4th instar became higher however between the 4th and the 5th became lower, moreover, the weight of female imago decreased, the life longevity of male and female imago were shorter and longer, respectively, along with the increase of B. thuringiensis infection. The possibility for resurgence will be discussed.Key words: B. thuringiensis, H. armigera, sublethal, resurgence
Tanggapan Helicoverpa armigera terhadap Infeksi Subletal Nuclear Polyhedrosis Virus dan Dampaknya terhadap Laju Reproduksi Pawana, Gita; Anggraeni, Tjandra; H.D., Anna Laksanawati
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12387

Abstract

The study on responses of Helicoverpa armigera Hbn. to sublethal HaNPV (H. armigera nuclear polyhedrosis virus) infection was carried out in the laboratory. The reproduction rate was scrutinized as their impact of HaNPVs exposures. Fifth instar of H. armigera larval was orally infected on sublethal serial dilution of HaNPV (1,800; 180; 18; 1.8; 0.18; 0.018 ppm from stock concentration of 1.1 X 10^9 PIB per ml suspension). A significantly female pupal weight forms the response of H. armigera to sublethal HaNPV infection on the concentration tested. However larval period, male pupal weight, pupal period, oviposition period, life span of imago, fecundity, fertility and survival did not significantly form the response of H. armigera to sublethal HaNPV infection. All treatment showed lower reproduction rate compared to the untreated, especially reproduction rate based on either debilitating effect and mortality or just based on debilitating effect.
Gonadal Maturity Induction using Karamunting (Melastoma malabatrhicum) Ethanol Extract on White Shrimp Female (Litopenaeus vannamei) Ridwan, Ahmad; Awaludin, Awaludin; Anggraeni, Tjandra
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Abstract

Common problem that happened in white shrimp culturing is insufficiency of mature female to provide the seed simultaneously. There are several methods to accelerate gonadal maturity infemale white shrimp: eye ablation, environmental manipulation, and providing high cholesterol fresh feed. Till now, high cholesterol feed is not very common method. Karamunting or Malabar Melastome (Melasthoma malabatrhicum) is known to have a high cholesterol content, more specifically, lanosterol based on GCMS test. Lanosterol is cholesterol that contained in plants, which assumed as precursor hormone for gonad maturity process in female of white shrimp. The aim of this experiment is to determine whether Karamunting ethanol extract can stimulate and accelerate gonad development in female of white shrimp. This experiment consists of several steps: (1) Karamunting (Melastoma malabathricum) extraction, (2) GCMS test for Karamunting, (3) extract injection to female of white shrimp (Litopenaues vannamel) for 15 days in 3 days interval with dosage variable 0 (control), ), 10 mg/kg BW (P1), 7,5 mg/kg BW (P2), 5 mg/kg BW (P3), 2 mg/kg BW (P4) dan 1 mg/kg BW (P5), (4) The measuring parameters are Karamunting content, morphological gonad development observation and hepatopancreas somatic index (HSI) measurement, (5) Data analysis. Data shows that karamunting ethanol extract with P1 and P2 dosage can result in morphological gonad development response (GML II), meanwhile with P3, P4 and P5 dosage there is no changes observed. All variables didn’t affect the level of HSI (P>0.05). Based on the result, it can be concluded that Karamunting ethanol extract indicates an acceleration of gonad maturity process in white shrimp female.Keywords : Gonad maturity; Hepatopancreas Somatic Index ; L.  vannamei; M. malabatrhicum
IDENTIFIKASI TIPE SEL HEMOSIT LARVA SERANGGA TRIGONA SP (HYMENOPTERA : APIDAE) DAN FUNGSINYA TERHADAP PERTAHANAN TUBUH Bay, Maria Marselina; Anggraeni, Tjandra
Bio-Edu: Jurnal Pendidikan Biologi Vol 1 No 3 (2016): Jurnal BIO-EDU Volume 1 Nomor 3 Tahun 2016
Publisher : Jurusan Pendidikan Biologi, Fakultas Ilmu Pendidikan, Universitas Timor

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Abstract

Tipe hemosit serangga telah banyak digambarkan, namun pengklasifikasian secara komperhensif masih sulit untuk dilakukan karena setiap individu serangga memiliki bentuk yang sangat berbeda pada berbagai kondisi. Secara umum tipe hemosit seranga dikelompokkan ke dalam enam jenis utama. Sel-sel itu adalah: prohemosit, plasmatosit, granulosit (yang mungkin sama seperti sistosit atau koagulosit), sel-sel spherule (spherulosit), oenositoid dan adipohemosit. Berdasarkan hal tersebut maka tujuan dilakukan penelitian ini adalah untuk mengetahui tipe sel hemosit larva serangga Trigona sp yang telah dilaporkan berperan sebagai vektor penyebab penyakit darah pada tanaman pisang. Penelitian dilaksanakan di Laboratorium Uji Hayati Serangga, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung. Hemolimf diambil dari pembuluh darah pada bagian punggung menggunakan jarum yang halus (Haemotokrit-Kapillaren). Kemudian sampel hemolimf diteteskan pada kaca objek yang telah diberi larutan turk. Untuk penghitungan total hemosit (Total Hemocytes Count (THC)) dihitung menggunakan mikroskop cahaya (pembesaran 40 X). Hasil identifikasi menunjukkan bahwa larva Trigona sp memiliki 5 jenis hemosit, yaitu : Plasmatosit, Prohemosit, Oenositoid, Sel sperul dan Granulosit. Berdasarkan penelitian dapat disimpulkan bahwa terdapat lima jenis tipe hemosit larva serangga Trigona sp, yaitu : plasmatosit, prohemosit, oenositoid, sel sperul dan granulosit.