RR. Endang Lukitaningsih
Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281

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Analytical Method Validation and Determination of Pyridoxine, Nicotinamide, and Caffeine in Energy Drinks Using Thin Layer Chromatography-Densitometry Riswanto, Florentinus Dika Octa; Lukitaningsih, RR. Endang; Martono, Sudibyo
Indonesian Journal of Chemistry Vol 15, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.601 KB) | DOI: 10.22146/ijc.21217

Abstract

Food supplement which contains vitamins and stimulants such as caffeine were classified as energy drink. TLC-densitometry method was chosen to determine the pyridoxine, nicotinamide, and caffeine in the energy drink sample. TLC plates of silica gel 60 F254 was used as the stationary phase and methanol : ethyl acetate : ammonia 25% (134:77:10) was used as the mobile phase. The correlation coefficient for each pyridoxine, nicotinamide, and caffeine were 0.9982, 0.9997, and 0.9966, respectively. Detection and quantitation limits of from the three analytes were 4.05 and 13.51 µg/mL; 13.15 and 43.83 µg/mL; 5.43 and 18.11 µg/mL, respectively. The recovery of pyridoxine, nicotinamide, and caffeine were within the required limit range of 95-105%. The percent of RSD were below the limit value of 5.7% for caffeine and nicotinamide and 8% for pyridoxine. The content amount of pyridoxine in the sample 1 and 2 were 33.59 ± 0.981 and 30.29 ± 2.061 µg/mL, respectively. The content amount of nicotinamide in the sample 1 and 2 were 106.53 ± 3.521 and 98.20 ± 3.648 µg/mL, respectively. The content amount of caffeine in the sample 1 and 2 were 249.50 ± 5.080 and 252.80 ± 2.640 µg/mL, respectively. Robustness test results showed that the most optimal method conditions should be applied for the analysis.
Penelusuran Fraksi Aktif Baccaurea lanceolata Dari Kabupaten Tapin, Kalimantan Selatan Sebagai Antioksidan Hadi, Samsul; Wahyuono, Subagus; Yuswanto, Ag.; Lukitaningsih, RR. Endang
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 12 No. 02 Desember 2015
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

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Abstract

ABSTRAK Baccaurea lanceolata oleh masyarakat Kalimantan Selatan digunakan untuk perawatan kulit, pendekatan ilmiah untuk membuktikan klaim itu salah satunya dengan uji antioksidan, uji antioksidan ini digunakan sebagai guided untuk menelusuri fraksi aktif. Dari hasil fraksinasi dengan menggunakan corong pisah, fraksi etil asetat mempunyai aktifitas paling tinggi dengan nilai IC50 230 ppm. Sehingga fraksi ini dilanjutkan difraksinasi dengan kolom gravitasi dan didapatkan 18 fraksi, fraksi No. 16 mempunyai aktifitas paling tinggi dengan nilai IC50 142 ppm dan fraksi No. 1 mempunyai aktifitas paling rendah dengan nilai IC50 675 ppm. Kata kunci: antioksidan, Baccaurea lanceolate, fraksi. ABSTRACT Baccaurea lanceolata by the people of South Borneo is used for skin care, scientific approach to prove the claim that one of them to antioxidant test, antioxidants test is used as guided to discover the active fraction. From the results of fractionation using a separating funnel, ethyl acetate fraction had the highest activity with IC50 values of 230 ppm. So that this continued fraction fractionated by column gravity and obtained 18 fractions, fractions No. 16 has the highest activity with value IC50 142 ppm and fractions No. 1 has the lowest activity with IC50 value of 675 ppm. Key words: antioxidant, Baccaurea lanceolata, fraction.