, Alimuddin
Department of Aquaculture

Published : 15 Documents
Articles

Found 15 Documents
Search

THE SEX RATIO AND TESTOSTERONE LEVELS IN TILAPIA IMMERSED IN DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE Junior, Muhammad Zairin; Naufal, Muhammad Restya; Setiawati, Mia; Hardianto, Dian; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3270.844 KB) | DOI: 10.19027/jai.16.1.51-59

Abstract

  Tilapia fish farming using monosex male population has been reported to have 10% higher productivity compared to mix-sex culture. This study aimed to determine immersion dose of 17?-metiltestosterone (MT) that allowed higher male percentage, growth, survival, and measure testosterone level in fish body. The experiment was designed as three immersion doses, namely: 0; 1.8; and 5.4 mg/L MT, each with three replications. Immersion was conducted to 14-day-old larvae for four hours, with density of 100 fish/L of water. Testosterone levels in fish was measured using ELISA method, and sex identification was performed by histology method. The result showed that percentage of male fish was the same in the treatment of 1.8 mg/L and 5.4 mg/L, and 53?65% higher than the control without MT treatment (54% male). Growth and survival of fish until day 56 post immersion were the same. By ELISA analysis, the levels of testosterone in larvae just after immersion was similar in 1.8 mg/L and 5.4 mg/L treatments, decreased drastically on day 14 after immersion, and then the levels of testosterone to be similar with the control on day 28, i.e. about 2 ng/g. By PCR method with specific primer, sex reversed and normal males could be distinguished, and on day 71 the testosterone levels in sex reversed and normal males fish was also the same, 0.3 ng/g (P>0.05). Thus, sex reversal by immersion at a dose of 1.8 mg/L can be consider as a standard protocol for monosex tilapia production. Testosterone level in the body of MT-treated fish becomes the same to the control of less than one month post immersion
DIGESTIVE ENZYMES ACTIVITIES IN OREOCHROMIS NILOTICUS FED DIET SUPPLEMENTED WITH RECOMBINANT GROWTH HORMONE Vinasyiam, Apriana; Suprayudi, Muhammad Agus; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3186.108 KB) | DOI: 10.19027/jai.15.15-23

Abstract

ABSTRACT The specific activity of the digestive enzymes, namely: pepsin, amylase, lipase, trypsin, and chymotrypsin were studied in Nile tilapia Oreochromis niloticus fed diet supplemented with recombinant Ephinephelus lanceolatus growth hormone (rElGH). The results showed that fish treated with rE1GH showed lower lipase and chymotrypsin specific activities (P>0.05), while the trypsin/chymotrypsin specific activity (T/C ratio) was found higher compared to control fish. Moreover, higher protein digestibility, higher protein retention and a lower ammonia excretion rate were measured for rE1GH treated fish. Oral rElGH administration enhanced Nile tilapia growth up to 20.04%, without affecting survival. This study suggested that rapid growth performance induced by rElGH was linked with T/C ratio rather than the specific activity of other digestive enzymes. Keywords: recombinant growth hormone, digestive enzyme, digestibility, Oreochromis niloticus  ABSTRAK Aktivitas spesifik enzim pencernaan pepsin, amilase, lipase, tripsin, dan kemotripsin diamati pada ikan nila Oreochromis niloticus yang diberi pakan mengandung hormon pertumbuhan rekombinan ikan kerapu kertang Ephinephelus lanceolatus (rElGH)). Hasil menunjukkan bahwa ikan uji pada perlakuan rElGH memiliki aktivitas spesifik enzim lipase dan kemotripsin yang lebih rendah, sedangkan rasio tripsin/kemotripsin (rasio T/C) yang lebih tinggi dibandingan ikan kontrol. Kecernaan protein dan retensi protein bernilai lebih tinggi sementara laju ekskresi amonia bernilai lebih rendah pada ikan perlakuan. Pemberian rElGH secara oral mampu mempercepat laju pertumbuhan ikan nila hingga 20,04% tanpa memengaruhi kelangsungan hidup. Berdasarkan penelitian ini, dapat disimpulkan bahwa laju pertumbuhan cepat yang diinduksi oleh rElGH berhubungan dengan rasio T/C dibandingkan dengan aktivitas spesifik enzim pencernaan lain. Kata kunci: hormon pertumbuhan rekombinan, enzim pencernaan, kecernaan, Oreochromis niloticus 
SEXUAL DIMORPHISM RELATED TO GROWTH IN CLIMBING PERCH ANABAS TESTUDINEUS Hidayat, Rahmat; Carman, Odang; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3170.908 KB) | DOI: 10.19027/jai.15.8-14

Abstract

 ABSTRCT The phenomenon that often occurs in climbing perch culture (Anabas testudineus, Bloch) is a significant size difference between individuals of male and female those lead to high variation in culture yields. In addition to genetic factors, the difference possibly reflects the sexual dimorphism in this fish; female grows faster than male. This research was conducted to examine sexual dimorphism related to growth quantitatively. Sixty individuals of 45-days old juvenile derived from mating of five pairs broods were randomly picked up and reared individually in 20×20×100 cm3 net cages for 135 days. Fish were fed on commercial feed (30% protein) three times a day at 10% feeding rate. Body weight and body length were individually recorded every 15 days, sex was determined at the end of the experiment by surgering the fish and gonad was weighed to calculate gonadosomatic index (GSI). The results showed that growth and specific growth rate of female were 1.17 and 1.48 fold respectively higher than male. This result indicates that female monosex culture of climbing perch is highly promising. Keyword: dimorphism, growth, climbing perch, Anabas testudineus  ABSTRAK Salah satu fenomena yang sering dijumpai dalam budidaya ikan papuyu (Anabas testudineus, Bloch) adalah perbedaan ukuran yang mencolok antara individu jantan dan betina yang menyebabkan hasil panen ikan sangat bervariasi. Selain faktor genetik, perbedaan ini diduga disebabkan oleh adanya dimorfisme seksual terkait pertumbuhan. Penelitian ini dilakukan untuk menguji dimorfisme seksual terkait dengan pertumbuhan ikan papuyu secara kuantitatif. Benih ikan umur 45 hari hasil pemijahan lima pasang induk diambil secara acak sebanyak 60 ekor dan dipelihara selama 135 hari secara individu di dalam 60 unit hapa (20×20×100 cm3). Ikan diberi pakan komersial (protein 30%) tiga kali sehari sebanyak 10% bobot tubuh. Bobot dan panjang semua ikan dicatat setiap 15 hari sekali, jenis kelamin ditentukan di akhir penelitian dengan cara membedah, mengamati gonad, dan menimbangnya untuk menentukan indeks gonadosomatik (IGS). Hasil penelitian membuktikan adanya dimorfisme seksual terkait pertumbuhan pada ikan papuyu; pertumbuhan bobot mutlak dan laju pertumbuhan bobot harian ikan betina masing-masing 1,48 kali dan 1,17 kali lebih tinggi dibandingkan dengan ikan jantan. Hasil penelitian ini mengindikasikan bahwa pengembangan budidaya ikan papuyu monoseks betina prospektif untuk dilakukan. Kata kunci: dimorfisme, pertumbuhan, papuyu, Anabas testudineus 
POTENTIAL TRANSMISSION TEST OF GP25 VACCINE IN NORMAL FLORA BACTERIA OF COMMON CARP CULTURE MEDIA Nuryati, Sri; Alimuddin, ,; Juliadiningtyas, Ayu Dhita
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3103.447 KB) | DOI: 10.19027/jai.14.90-97

Abstract

ABSTRACT Koi herpesvirus (KHV) is a virus that infects common carp and koi Cyprinus carpio. KHV attacks most stadia of common carp and koi and causes mortality up to 80?95% of the population. One way to prevent the spread of KHV is  by applying DNA vaccine. This research was conducted to test potential transmission of DNA vaccine encoding glycoprotein 25 (vaccine GP25) toward normal flora bacteria of media cultivation of common carp. Bacteria was isolated from pond water of common carp and tested for their sensitivity to ampicillin. Research was performed by adding vaccine GP25 to ampicillin-sensitive bacteria at a dose of 12.5 µg/100 µL and incubated at 28 °C for 30, 60, 180, and 300 minutes then plated on media containing ampicillin. The grown bacteria cells were tested for the existence of plasmid bearing gen GP25 through bacteria colony cracking. The results of this research showed that there was no bacteria contained plasmid bearing gen GP25. Keywords: DNA vaccines, common carp, vaccine safety  ABSTRAK Koi herpesvirus (KHV) merupakan virus yang menginfeksi ikan mas dan koi Cyprinus carpio. KHV menyerang hampir semua stadia ikan mas dan koi dan menyebabkan kematian hingga 80?95% dari populasi. Salah satu cara penanggulangan penyebaran KHV adalah dengan penggunaan vaksin DNA. Penelitian ini dilakukan untuk menguji potensi transmisi vaksin DNA glikoprotein 25 (vaksin GP25) pada bakteri flora normal pada media budidaya ikan mas. Bakteri diisolasi dari kolam budidaya ikan mas dan diuji sensitivitasnya terhadap antibiotik ampisilin. Penelitian dilakukan dengan menambahkan vaksin GP25 ke larutan bakteri sensitif ampisilin dengan dosis 12,5 µg/100 µL dan diinkubasi pada suhu 28 °C selama 30, 60, 180, dan 300 menit kemudian disebar pada media mengandung antibiotik ampisilin. Sel bakteri yang tumbuh diuji untuk mendeteksi keberadaan plasmid pembawa gen GP25 dengan menggunakan metode seleksi koloni bakteri (colony cracking). Hasil penelitian ini menunjukkan bahwa tidak ada bakteri yang mengandung plasmid pembawa gen GP25. Kata kunci: keamanan vaksin, ikan mas, vaksin DNA
INDUCED MATURATION OF EEL ANGUILLA BICOLOR USING DIFFERENT HORMONE COMBINATION Sudrajat, Agus Oman; Sugati, Antharest; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3147.544 KB) | DOI: 10.19027/jai.13.189-201

Abstract

ABSTRACT Artificial reproduction of eel Anguilla bicolor is not yet well-established because of insufficient broodstock number. In this research, induction of Indonesian eel gonad maturation was performed by hormonal with a combination of pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) antidopamin and recombinant growth hormone (rGH). This research consisted of five treatments namely: control (NaCl 0,9%), PMSG 20 IU/ kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg + rGH 10 ?g/kg dan PMSG 20 IU/kg + HCG 10 IU/kg. Each treatment contained 10 fishes. Hormonal induction was conducted by intramuscular injections, as much as five times at intervals of seven days. Furthermore observations on gonadal development were performed after injection for 21 days. The results showed that the treatment generated pregnancy level of 100%, while control was 0%. The best treatment was PMSG 20 IU/kg + antidopamin 10 ppm/kg+ rGH 10 ?g/kg, seen from a more mature phase of the gametes, spermatocytes in male and oocytes with perinukleolar phase in female fish. Eel at the body weight of 120.4 to 207.8 g and at the body length of 40.9 to 43.1 cm was male, at the body weight of 274.8 g and at the body length of 47 cm was in intersexual phase, and at the body weight of 323.4 g and at the body length of 53 cm was female. Keywords: Anguilla bicolor, antidopamin, hormones, PMSG, rGH, HCG  ABSTRAK Pemijahan ikan sidat secara buatan belum dapat dilakukan karena keterbatasan induk matang gonad. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian hormon terhadap percepatan proses perkembangan gonad ikan sidat (Anguilla bicolor). Hormon yang digunakan adalah kombinasi dari pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG), antidopamin dan recombinant growth hormone (rGH). Induksi hormonal untuk mempercepat perkembangan gonad ikan sidat dilakukan melalui lima perlakuan yaitu yaitu kontrol (NaCl 0,9%), PMSG 20 IU/kg, PMSG 20 IU/kg+antidopamin 100 ppm/kg, PMSG 20 IU/kg+antidopamin 100 ppm/ kg+rGH 10 ?g/kg dan PMSG 20 IU/kg+HCG 10 IU/kg. Setiap perlakuan dilakukan pada sepuluh ekor ikan sidat. Aplikasi induksi hormonal dilakukan melalui penyuntikan secara intramuskular sebanyak lima kali dengan interval tujuh hari sekali, selanjutnya dilakukan pengamatan terhadap perkembangan gonad selama 21 hari dengan interval tujuh hari sekali setelah penyuntikan. Hasil penelitian menunjukkan bahwa perlakuan hormonal menyebabkan tingkat kebuntingan sebanyak 100% pada ikan perlakuan, sedangkan kontrol sebanyak 0%. Kombinasi terbaik adalah PMSG+antidopamin+rGH, terlihat dari fase gamet yang lebih matang yaitu mencapai fase spermatosit pada ikan jantan dan oosit dengan fase perinukleolar pada ikan betina. Berdasarkan hasil penelitian, ikan sidat dengan bobot 120,4?207,8 g dan panjang 40,9?43,1 cm masih berjenis kelamin jantan. Ikan dengan bobot 274,8 g dan panjang 47 cm masih berada pada fase peralihan kelamin, sedangkan pada bobot 323,4 dan panjang 53 cm sudah berjenis kelamin betina. Kata kunci: Anguilla bicolor, antidopamin, hormon, PMSG, rGH, HCG
SURVIVAL OF COMMON CARP CARRYING CYCA-DAB1*05 POST-CHALLENGED WITH AEROMONAS HYDROPHILA Arsal, Laode Muhammad; Yuhana, Munti; Nuryati, Sri; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2994.053 KB) | DOI: 10.19027/jai.13.167-178

Abstract

ABSTRACT Blood parameters are considered as important indicators to diagnose fish health status. This study was performed to observe blood profiles including total erythrocytes, hemoglobin concentration, hematocrite, total leukocytes and differential leukocytes, and survival of common carp Cyprinus carpio infected by Aeromonas hydrophila. Fish were divided into two groups: the 3rd generation of common carp carrying fish carrying Cyca-DAB1*05 of major histocompatibility complex II molecular marker, and fish without the marker as control treatment. histocompatibility complex II molecular marker, and fish without the marker as control treatment. Common carp 3rd generation was produced by crossing among second generation of fish carrying the Cyca-DAB1*05 marker. Each fish was injected intramuscularly by 0.1 mL of 108 cfu/mL A. hydrophila. Challenge test was conducted for 14 days and blood was collected at day-0, three, seven, and 14. The results of this study showed that erythrocytes, hemoglobin and hematocrite concentrations of common carp carrying the molecular marker at post challenge with A. hydrophila were higher (P<0.05) compared to control fish. The blood profiles were highly correlated to survival of fish. Survival of fish that carrying the molekuler marker was about two point six fold higher than those of control fish. Keywords: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, molecular marker  ABSTRAK Gambaran darah merupakan indikator penting untuk mendiagnosa penyakit ikan. Penelitian ini dilakukan untuk meguji gambaran darah ikan mas Cyprinus carpio setelah diinfeksi dengan bakteri Aeromonas hydrophila yang meliputi total sel darah merah, konsentrasi hemoglobin, hematokrit, total sel darah putih, dan diferensial leukosit, serta sintasannya. Ikan mas yang digunakan terdiri atas ikan mas generasi ketiga yang membawa marka molekuler Cyca-DAB1*05 dari kelompok major histocompatibility complex II dan ikan mas tanpa marka sebagai kontrol. Ikan mas generasi ketiga merupakan keturunan persilangan antarikan mas generasi kedua yang mempunyai marka Cyca-DAB1*05. Masing-masing ikan diinfeksikan A. hydrophila secara intramuskuler pada dosis 0,1 mL, kepadatan 108 cfu/mL. Uji tantang dilakukan selama 14 hari, dan sampel darah ikan diambil pada hari ke-0, tiga, tujuh dan 14. Hasil uji tantang menunjukkan bahwa total sel darah merah, konsentrasi hemoglobin dan hematokrit pada ikan mas yang membawa marka molekuler lebih tinggi dibandingkan ikan mas tanpa marka (P<0,05). Hasil uji gambaran darah berkorelasi tinggi dengan tingkat kelangsungan hidup ikan mas. Tingkat kelangsungan hidup ikan mas yang membawa marka molekuler Cyca-DAB1*05 lebih tinggi hingga dua koma enam kali daripada ikan mas kontrol. Kata kunci: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, marka molekuler 
SEX REVERSAL ON CONGO TETRA FISH (MICRALEPTUS INTTERRUPTUS ) LARVAE Arfah, Harton; Alimuddin, ,; Sumantadinata, K.; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.65 KB) | DOI: 10.19027/jai.1.69-74

Abstract

ABSTRACTExperiment was performed to assess the effect of 17a-methyltestosterone (MT) treatment on Congo tetra fish larvae.  To evaluate the optimal pattern of MT treatment, three different treatments were administrated.  Three months old larvae were submerged in three different doses of MT; 1, 2 and 4 mg/l.  These studies showed that the highest percentage of male fish was obtained by 4 mg/l MT treatment, 87,17%.  The 2 mg/l and 1 mg/l MT treatments obtained 77,53% and 69,86% male respectively, two times higher than control, 38,96%.  On the other hand, the 4 mg/l MT treatment also resulted the highest percentage of hermaphrodite fishes, 17,58%.  The highest survival rate was shown by 1 mg/l MT treatment, 62,77% and the lowest was shown by the 4 mg/l MT treatment, 47,20%.  The highest rate of fish length and weight was shown by the 4 mg/l MT treatment, 4,4 cm and 1,65 gram respectively.  These findings suggest that MT treatment offers an advantage in growth of  tetra Congo larvae. Key word :  Sex reversal, methyltestosterone, Congo tetra fish, Micraleptus intterruptus. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perendaman larva di dalam larutan hormon 17a-metiltestosteron pada dosis 1, 2 dan 4 mg/l larutan.  Persentase tertinggi ikan jantan dihasilkan  oleh perlakuan 4 mg/l, yaitu 87,17%.  Perlakuan 2 mg/l dan 1 mg/l masing-masing menghasilkan 77,53% dan 69,86% sedangkan kontrol menghasilkan 38,96% jantan.  Efek lain dari perlakuan MT ini adalah hermafroditisme.  Perlakuan 4 mg/l menghasilkan persentase hermafrodit tertinggi yaitu 17,58%, sedangkan pada kontrol kelangsungan hidup tertinggi diperoleh pada perlakuan 1 mg/l (62,77%) dan terendah pada perlakuan 4 mg/l (47,20%).  Hasil tersebut menunjukkan adanya pengaruh dosis hormon terhadap kelangsungan hidup ikan.  Pengukuran bobot dan panjang ikan pada setiap perlakuan menunjukkan nilai tertinggi dihasilkan oleh perlakuan 4 mg/l  yaitu 1,65 gram dan 4,40 cm.  Hal ini diduga bahwa hormon MT merangsang pula pertumbuhan ikan.Kata kunci :  Pergantian kelamin, metiltestosteron, ikan tetra Kongo, Micraleptus intterruptus.
PHENOTYPE OF THE FIRST GYNOGENESIS GENERATION OF KOI Alimuddin, ,; Sumantadinata, K.; Hadiroseyani, Yani; Irawan, D.
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.035 KB) | DOI: 10.19027/jai.1.65-68

Abstract

ABSTRACTThis experiment was conducted to study phenotype of F1 koi that obtained from gynogenesis at the Laboratory of Fish Genetic and Breeding, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University (IPB). Females koi used for this experiment were kohaku (white-red), hi-utsuri (red-black), and shiro-bekko (white-black); whereas males used kohaku, hi-utsuri, and shiro-bekko. Analysis on body coloration of fish was carried out at three months old. Results showed that gynogenesis from kohaku produced three types of koi, those were white koi, red koi and kohaku, and hi-utsuri produced red koi, black koi and hi-utsuri. Meanwhile, shiro-bekko by gynogenetic technique produce seven types of koi; those were white, red, black, kohaku, shiro-bekko, hi-utsuri and sanke (white-red-black koi). Survival rate of gynogenetic koi was lower then normal might be due to inbreeding stress.Key words :  Gynogenesis, phenotype, koi fish (Cyprinus carpio). ABSTRAKStudi tentang fenotip keturunan pertama ikan koi hasil ginogenesis telah dilakukan di Laboratorium Pengembangbiakan dan Genetika Ikan, Jurusan Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan IPB. Ikan koi betina yang dipakai adalah kohaku (putih-merah), hi-utsuri (merah-hitam) dan shiro-bekko (putih-hitam), sedangkan jantannya adalah kohaku, hi-utsuri, dan shiro-bekko. Analisis warna pada ikan dilakukan setelah ikan berumur tiga bulan. Hasil penelitian menunjukkan bahwa ginogenesis pada ikan kohaku menghasilkan tiga jenis ikan koi, yaitu koi putih, koi merah dan kohaku; pada ikan hi-utsuri dihasilkan ikan koi merah, koi hitam dan hi-utsuri. Sementara itu, teknik ginogenesis untuk ikan koi putih-hitam dihasilkan tujuh macam jenis ikan koi, yaitu koi putih, koi merah, koi hitam, kohaku, hi-utsuri, siro-bekko dan sanke (putih-merah-hitam). Tingkat kelangsungan hidup ikan ginogenetik lebih rendah daripada kontrol normalnya.Kata kunci :  Ginogenesis, fenotip, ikan koi (Cyprinus carpio)
AROMATASE GENE EXPRESSION AND MASCULINIZATION OF NILE TILAPIA IMMERSED IN WATER 36 °C CONTAINING 17α-METHYLTESTOSTERONE Fauzan, Agung Luthfi; Soelistyowati, Dinar Tri; Junior, Muhammad Zairin; Hardiantho, Dian; Setiawati, Mia; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3315.516 KB) | DOI: 10.19027/jai.16.1.116-123

Abstract

ABSTRACT  Immersion of undifferentiated larval tilapia in high temperature and 17?-methyltestosterone (MT) cab increase the male ratio. However, the effectiveness of immersion in high temperature of water containing MT remains to be evaluated. The purposes of this study were: 1) evaluate the male ratio, growth, and survival of tilapia, and 2) analyze the aromatase brain-type gene expression level in tilapia after immersing in high temperature (36 °C) containing MT at 2 mg/L for four hour with single and double immersion. Aromatase gene expression was analyzed by semi-quantitative RT-PCR (sqRT-PCR) method. The result showed that higher monosex male ratio was obtained by single immersion of MT at 36 °C at room temperature. Gene expression level of aromatase brain-type was lower on single immersion and increased significantly at second immersion compared to control (immersion at room temperature without MT). Immersion using MT and high temperature had no significant effect on fish survival. However the specific growth rate and fish biomass were higher than control. Thus, monosex male tilapia can be produced by single immersion of undifferentiated larvae at 36 °C temperature containing MT. Keywords: male ratio, aromatase, Oreochromis niloticus, temperature, 17?-methyltestosterone  ABSTRAK  Perendaman larva ikan nila yang belum terdeferensiasi kelaminnya dengan suhu tinggi dan hormon 17?-metiltestosteron (MT) dapat meningkatkan nisbah kelamin jantan. Tetapi, efektivitas perendaman menggunakan MT pada suhu tinggi belum diteliti. Tujuan dari penelitian ini adalah 1) mengevaluasi nisbah kelamin jantan, pertumbuhan, dan kelangsungan hidup ikan nila, dan 2) menganalisis ekspresi gen aromatase tipe-otak pada ikan direndam menggunakan MT dengan dosis 2 mg/L selama empat jam sebanyak satu dan dua kali perendaman pada suhu 36 °C. Ekspresi gen aromatase dianalisis menggunakan metode RT-PCR semi-kuantitatif (sqRT-PCR). Hasil penelitian menunjukkan bahwa kombinasi perendaman MT satu kali pada suhu 36 °C lebih tinggi menghasilkan ikan nila jantan monoseks dibandingkan perendaman MT satu kali pada suhu ruang. Tingkat ekspresi gen aromatase tipe otak pada perendaman satu kali lebih rendah, dan meningkat secara signifikan pada perendaman kedua dibandingkan dengan kontrol (perendaman pada suhu ruang tanpa MT). Perendaman larva menggunakan MT dan suhu 36 °C tidak berpengaruh nyata terhadap kelangsungan hidup, tetapi laju pertumbuhan spesifik dan biomassa ikan perlakuan tersebut lebih tinggi daripada kontrol. Dengan demikian, ikan nila jantan monoseks dapat diproduksi dengan perendaman satu kali pada larva yang belum terdeferensiasi jenis kelaminnya menggunakan MT pada suhu 36 °C. Kata kunci: rasio jantan, aromatase, Oreochromis niloticus, suhu, 17?-metiltestosteron
FREQUENCY AND PERSISTENCY OF DNA VACCINE ENCODING GP25 BY ORAL ON COMMON CARP Nuryati, Sri; Yuliyanti, ,; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3394.616 KB) | DOI: 10.19027/jai.12.150-157

Abstract

ABSTRACT Koi herpesvirus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak is happened in almost all centre of common carp culture in Indonesia and caused mass mortality. Deoxyribonucleic acid (DNA) vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. The aim of this research was to get frequency and persistency of DNA vaccine encoding GP25 given by oral delivery method in common carp. This research would like to determine dose, frequency of vaccination, persistency of DNA vaccine and culture medium for the bacterial host. DNA vaccine persistency test was done by using polymerase chain reaction (PCR) method with the specific primer for GP25 gene. The results showed that level of DNA vaccine that could be detected in feed was 7.56 ng (equal to 1.598×1010 copies). Efficient culture medium for Escherichia coli DH5? carrying DNA vaccine was LB triptone. Feeding fish with diet supplemented with 1 mL E. coli DH5? containing DNA vaccine for each fish and two times a week allowed persistence of DNA vaccine in kindney and spleen. Keywords: common carp, KHV, DNA vaccine, GP25, persistance  ABSTRAK Koi herpesvirus (KHV) adalah virus patogen utama yang menginfeksi ikan mas dan ikan koi. Wabah penyakit KHV terjadi di hampir semua sentra budidaya ikan mas di Indonesia dan menyebabkan kematian massal ikan. Metode vaksinasi DNA merupakan salah satu cara yang dapat dilakukan untuk menanggulangi serangan KHV. Pemberian vaksin umumnya dilakukan dengan cara injeksi. Tujuan penelitian ini adalah untuk menguji frekuensi dan persistensi vaksin DNA GP25 antivirus KHV yang diberikan melalui oral pada ikan mas. Pada penelitian ini dilakukan uji dosis, frekuensi pemberian vaksin, persistensi vaksin DNA, dan media kultur bakteri inang. Persistensi vaksin DNA dianalisis menggunakan metode PCR dengan primer spesifik gen GP25. Hasil penelitian menunjukkan bahwa dosis vaksin DNA yang dapat terdeteksi dalam pakan adalah 7,56 ng (setara dengan 1,598×1010 copy). Media kultur yang efisien bagi bakteri Escherichia coli DH5? pembawa vaksin DNA adalah LB tripton. Pemberian pakan bervaksin berupa bakteri konsentrasi 1 mL/ekor ikan dengan frekuensi dua kali seminggu menghasilkan persistensi DNA GP25 di ginjal dan limpa. Kata kunci: ikan mas, KHV, vaksin DNA, GP25, persistensi