Fitmawati Fitmawati
Kampus Binawidya Panam Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau

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EFEKTIVITAS IMUNOMODULATOR EKSTRAK DAUN MACANG (MANGIFERA FOETIDA L.) TERHADAP SEL MAKROFAG TIKUS PUTIH (RATTUS NORVEGICUS) Kholifah, Sri Nur; Fitmawati, Fitmawati
Jurnal Pendidikan Matematika dan IPA Vol 11, No 1 (2020): Januari 2020
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jpmipa.v11i1.32763

Abstract

Macang (Mangifera foetida L.) has potential as an effect herbal medicinal plant with antioxidant content, namely mangiferin. The aim of this study is to prove empirically study that macang has an immunomodulatory effect through preclinical testing. This study use white mice (Rattus norvegicus) as experimental animals and Staphylococcus aureus as test bacteria. Rats were give leaf extracts of 3 varieties of orally for 7 days using 3 prescribe doses in ppm. On the 8th day, each rats was induce by an intraperitoneal section with 0,5 ml of the bacterial suspension tested. Observation of peritoneal fluid through smear preparations includes 2 parameters, phagocytosis activity and capacity. The result showed that the immunomodulatory effects on batu varieties were effective at dose 2 (10 ppm = 0,01 mg/g), limus varieties and manis varieties were effective at dose 1 (1 ppm = 0,001 mg/g). According to this research, it was concluded that leaf extracts of three varieties macang could be used as immunomodulators.AbstrakMacang (Mangifera foetida L.) mempunyai potensi sebagai tumbuhan obat herbal dengan kandungan antioksidan yaitu mangiferin.  Tujuan penelitian ini adalah membuktikan efek imunomodulator pada tiga varietas macang (batu, limus dan manis) melalui uji praklinik.  Penelitian ini menggunakan tikus putih (Rattus norvegicus) sebagai hewan uji dan Staphylococcus aureus sebagai bakteri uji.  Tikus diberi ekstrak daun tiga varietas macang secara oral selama tujuh hari menggunakan tiga serial dosis dalam satuan ppm.  Pada hari ke-8, setiap tikus putih diinduksi bagian intraperitoneal dengan 0,5 ml suspensi bakteri uji. Pengamatan cairan peritoneal melalui preparat apusan meliputi 2 parameter yaitu aktivitas dan kapasitas fagositosis.  Hasil penelitian menunjukkan bahwa efek imunomodulator pada varietas batu efektif pada dosis 2 (10 ppm=0,01 mg/g), varietas limus dan manis efektif pada dosis 1 (10 ppm=0,001mg/g). Disimpulkan bahwa daun macang (Mangifera foetida) berpotensi digunakan sebagai imunomodulator.Kata kunci : Fagositosis makrofag, Imunomodulator, Mangifera foetida
KEANEKARAGAMAN DURIAN (Durio zibethinus Murr.) DI PULAU BENGKALIS BERDASARKAN KARAKTER MORFOLOGI Lestari, Sri; Fitmawati, Fitmawati; Nihayatul Wahibah, Ninik
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) Juli 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Bengkalis Island is one of the areas with high diversity of durian (Durio zibethinus Murr.). This research was aimed to analyze the diversity of  durian cultivars of Bengkalis Island. It was conducted from April up to August 2010. The morphological data were analysed using NTSYS-pc 2.02 and minitab 14.13 softwares. Genetic similarity value among 36 accesions ranged from 0,28 (between BT 14, BT 15 and SB6) to 0,66 (between BT6 and BT7). The cluster analysis showed that durian diversity from Bengkalis island was 34-58% or had similarity level 42-66% and formed 7 main groups. However, they were ungroupped depend on their growth areas. Based on PCA (Principal Components Analysis) value, durian diversity was 99,7% and formed 6 groups.  Furthermore, the result of pearson correlation analysis among 77 character showed that 10 characters were positively correlated and 4 characters were negatively correlated.
JENIS-JENIS CEMPEDAK (Artocarpus champaden Lour.) DI KABUPATEN KAMPAR PROVINSI RIAU Fitmawati, Fitmawati; Andani, Via; Sofiyanti, Nery
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 3 No 1 (2018): Ekotonia: Journal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (777.079 KB) | DOI: 10.33019/ekotonia.v3i1.756

Abstract

Cempedak (Artocarpus champaden Lour.) is one of the special fruits in Southeast Asia which is the prima donna of the community. In the province of Riau, cempedak found in many areas of Kampar regency. This study aims to identify the types of cempedak that exist in Kampar regency. Based on the results of the research, cempedak is categorized into four cultivars ie cempedak pulp cultivars, cempedak forest cultivars, cempedak langkat cultivars and cempedak nangkadak cultivars, from four cempedak cultivars found, Nempadak cempedak cultivars and Langkat cultivars are the most superior cempedak cultivars because they have fruits big, lots of content, thick meat and sweet taste.
KEANEKARAGAMAN DURIAN (Durio zibethinus Murr.) DI PULAU BENGKALIS BERDASARKAN KARAKTER MORFOLOGI Lestari, Sri; Fitmawati, Fitmawati; Nihayatul Wahibah, Ninik
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) July 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bengkalis Island is one of the areas with high diversity of durian (Durio zibethinus Murr.). This research was aimed to analyze the diversity of  durian cultivars of Bengkalis Island. It was conducted from April up to August 2010. The morphological data were analysed using NTSYS-pc 2.02 and minitab 14.13 softwares. Genetic similarity value among 36 accesions ranged from 0,28 (between BT 14, BT 15 and SB6) to 0,66 (between BT6 and BT7). The cluster analysis showed that durian diversity from Bengkalis island was 34-58% or had similarity level 42-66% and formed 7 main groups. However, they were ungroupped depend on their growth areas. Based on PCA (Principal Components Analysis) value, durian diversity was 99,7% and formed 6 groups.  Furthermore, the result of pearson correlation analysis among 77 character showed that 10 characters were positively correlated and 4 characters were negatively correlated.
VARIASI INFRASPESIES MACANG (MANGIFERA FOETIDA) BERDASARKAN SEKUEN GEN RBCL Rafidah, Nurul; Fitmawati, Fitmawati; Juliantari, Erwina; Sofiyanti, Nery
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v12i1.5516

Abstract

AbstrakMacang (Mangifera foetida) adalah jenis mangga kosmopolit dengan vigoritas yang kuat. Macang banyak ditemukan di perkarangan dan kebun dan bersifat semi liar. Penurunan luas lahan seperti deforestasi dapat mengakibatkan keanekaragaman kultivar macang juga mengalami penurunan secara cepat. Penelitian ini bertujuan untuk menganalisis dan merekonstruksi hubungan kekerabatan antar kultivar macang menggunakan sekuen gen rbcL. Sampel berasal dari koleksi hasil eksplorasi mangga Sumatera bagian Selatan, yaitu Provinsi Bengkulu, Lampung dan Sumatera Selatan. Ekstraksi DNA dilakukan dengan menggunakan metode CTAB yang dimodifikasi, kemudian DNA diamplifikasi dengan menggunakan primer spesifik rbcL forward dan reverse, dan dilakukan sekuensing serta analisis filogenetik. Rekontruksi pohon filogenetik menggunakan program PAUP* versi 4.0b10 dengan metode Maximum Parsimony (MP) dan Neighbor Joining (NJ). Kladogram dengan MP diperoleh dua klad, yaitu pada klad I terdiri dari kultivar Macang lonjong dan Macang lado, sedangkan klad II terdiri dari kultivar Macang bulat. Berdasarkan metode NJ, diperoleh Macang bulat memiliki jarak genetik lebih panjang sehingga dianggap sebagai individu yang lebih primitif daripada kultivar yang lain. Dengan demikian, dari penelitian ini diperoleh informasi dan bukti dari status taksonomi kultivar macang.Abstract Mangifera foetida is a species of cosmopolitan mango with strong vigor. Some M. foetida are found in some front houses and gardens and has a character of semi-wild in its cultivation. Decreases in land area, such as deforestation, can lead to a rapid decline in their diversity of cultivars. This study aimed to analyze and reconstructs the phylogenetic relationship among M. foetida cultivars using the rbcL gene sequences. Samples were collected from the exploration within the area of South Sumatra, such as Provinces of Bengkulu, Lampung and South Sumatra. The DNA extraction was carried out using the modified CTAB method, followed by DNA amplification using rbcL-specific primers, sequencing, and phylogenetic analysis. The phylogenetic trees were reconstructed using the PAUP* version 4.0b10 by using the method of Maximum Parsimony (MP) and Neighbor Joining (NJ). Cladogram of the MP tree showed two clades that the clad I consisted of M. foetida (Macang lonjong) and M. foetida (Macang lado) cultivars, whereas clad II consisted of M. foetida (Macang bulat) cultivar. The NJ tree showed that M. foetida (Macang bulat) has a longer genetic distance so it is considered as a more primitive cultivar than others. Therefore, information and evidences from the taxonomic status of the M. foetida cultivars were obtained from this study.
Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus) Fitmawati, Fitmawati; Roza, Rodesia Mustika; Sofiyanti, Nery; Isnaini, Isnaini; Fitri, Febrian Lailatul; Paramita, Desi; Dewi, Awal Prichatin Kusumo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10496

Abstract

Obat pahit has been generally known and believed by Lingga Malay society as anti-aging agent. However, the study of Obat pahit is not scientifically proven. This research was aimed to prove immunomodulatory ability of Obat pahit potion from Lingga, Riau Archipelago. This study used white rats as an animal modelling, and Staphylococcus aureus as bacteria tester. The rats had been treated with aqueous Obat pahit extract from three TMPs on dose scales of 0.09, 0.18 and 0.27 mL/200g of body weight through oral administration for 7 days. Furthermore, on the 8th days, the experiment animals were injected by the preparation of bacteria tester through intraperitoneal administration in the amount of 0.5 mL/200 gram of body weigth and subsequently incubated for 1 hour after the injection. There were 2 observed parameters on this study, i.e efectivity and capacity of phagocytosis by leukocytes. The observation of leukocytes-phagocytocis activity was carried out by making a smear preparat samples of peritoneum fluid from rats. After the observation under microscope on a magnification of 100 times. The result was obtained the Obat pahit from Kalan PMT swere more effective on dose 2, while from SP4 and Linau TMPs were much more effective on dose 1. It is therefore, using these data of the results, the advanced doses scale of this Obat pahit would not be necessary. Obat pahit potion from Malay Lingga Malay Ethnic could become raw materials of immunomodulatory herbal medicine based on traditional knowledge. It also potentially as a standardized herbal.
Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago Fitmawati, Fitmawati; Sofiyanti, Nery; Roza, Rodesia Mustika; Isnaini, Isnaini; Irawan, Yulisa Resti; Winata, Dhaniel Ridho; Dewi, Awal Prichatin Kusumo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9808

Abstract

Obat Pahit is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely Bauhunia semibifida, Cnestis palala and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in Obat Pahit from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of Obat Pahit from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of Bauhinia semibifida (6.6247), Penawa Root (5.0124) and Cnestis palala (5.9968) were much lower than IC50 of mangosteens rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesias local ethnicities.
Histology Structure of Lymph and Uterus of White Rat (Rattus norvegicus) Given with Nanas Bongsai (Ananas comosus var. microstachys L.) Fitmawati, Fitmawati; Saputra, Agus; Yohanes, Yohanes; Teruna, Hilwan Yuda; Nugraha, Dimas Pramita; Hamidi, Yulis
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13596

Abstract

Nanas Bongsai (Ananas comosus var. microstachys L.) is an amazing herb which is used in traditional medicine by local people of Muara Lembu district as a potion to reduce pain while menstruation period for a women. The present work was designed to investigate its probable side effects on the histopathologic changes in limph and uterus tissues after treated with A. comosus var. microstachys L extract in female white rats. This is an experimental research consisted of five treatments and three repetitions. Treatment composed of two controls (P0 and P+) and given Nanas Bongsai extract with three different dosages. Histology result of lymph show that all treatments given with Nanas Bongsai with three different dosages have different diameter average of white pulp and there is no significant changes with normal control. While observation towards histology structure of endometrium thickness with dosage P1, dosage P2, dosage P3 is 206.333±33.486; 215.667±33.486; 197.667±60.871 respectively. The most thin endometrium layer found in treatment with dosage P3 compared to control P0. From this study showed that there is no toxic effect on uterus and lymph function of of Nanas Bonsai (A. comosus var. microstachys L.) at different doses, so the plant is secure for consumption by the community.
STRUKTUR HISTOLOGI HATI TIKUS PUTIH (Rattus norvegicus Berkenhout 1769) DENGAN PEMBERIAN RAMUAN TRADISIONAL MASYARAKAT MELAYU LINGGA, KEPULAUAN RIAU Fitmawati, Fitmawati; Titrawani, Titrawani; Safitri, Welly
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 3 No 1 (2018): EKOTONIA: Jurnal Penelitian Botani, Zoologi dan Mikrobiologi
Publisher : Jurusan Biologi, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.924 KB)

Abstract

Lingga Island is the center of the Malay kingdom of Kepulauan Riau Province with local wisdom that is still ingrained in the community. Obat pahit is a decoction of linguistic stew of ethnic lingga community which is believed to be a youthful remedy and maintain stamina. This study aims to find histopathologic changes of liver white mice after the decoction of herb concoction of Obat pahit. The percentage of intercellular damage was nonexistent from the normal control group and positive control but different from the negative control group. Different types of medicinal herbs with different dosage levels. But the damage is still in the normal, accessible category that has no toxic effects from bitter herbs on liver organ
JENIS-JENIS CEMPEDAK (Artocarpus champaden Lour.) DI KABUPATEN KAMPAR PROVINSI RIAU Fitmawati, Fitmawati; Andani, Via; Sofiyanti, Nery
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 3 No 1 (2018): EKOTONIA: Jurnal Penelitian Botani, Zoologi dan Mikrobiologi
Publisher : Jurusan Biologi, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.924 KB)

Abstract

Cempedak (Artocarpus champaden Lour.) is one of the special fruits in Southeast Asia which is the prima donna of the community. In the province of Riau, cempedak found in many areas of Kampar regency. This study aims to identify the types of cempedak that exist in Kampar regency. Based on the results of the research, cempedak is categorized into four cultivars ie cempedak pulp cultivars, cempedak forest cultivars, cempedak langkat cultivars and cempedak nangkadak cultivars, from four cempedak cultivars found, Nempadak cempedak cultivars and Langkat cultivars are the most superior cempedak cultivars because they have fruits big, lots of content, thick meat and sweet taste