Articles

Found 7 Documents
Search

OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE Herawati, Maria Marina; Pudjihartati, Endang; Pramono, Suwijiyo; Sulistyaningsih, Endang; Purwantoro, Aziz
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.Keywords: 2,4-D, Artemisia cina, BA, polyploidy
THE EFFECT OF SOAKING SALTS CONCENTRATION TO CANDIED GARLIC (SATIVUM ALLIUM L.) Titaley, Naning Restiani; Herawati, Maria Marina
Jurnal Ilmu Pangan dan Hasil Pertanian Vol 3, No 2 (2019)
Publisher : Program Studi Teknologi Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/jiphp.v3i2.4947

Abstract

Garlic has a bitter taste, which makes people reluctant to eat one whole garlic clove. The salt soaking technique used to reduce the bitter taste of the garlic, and the garlic processed into wet candied garlic, so it became more edible than not process. The process of candied garlic could preserve the durability level, which allows it to be stored longer at low temperatures. The research materials used were salt and sugar. The purpose of the research was to know the effect of salt concentration on reducing bitterness and the right concentration to reduce the bitter taste of garlic. This research used the RAK method, and DMRT (Duncan?s Multiple Range Test), and the samples of salt were 3%; 3.5%; 4%; 4.5%. The test included chemical tests (water content and total microbes) and organoleptic tests (taste, aroma, and texture). The water content of the product showed the result, which was not significantly different, and the total microbes test showed no microorganism was found or grew on the product. The organoleptic test of taste was obtained on 3.5% salt, with an average of 2.75% (likes), while the aroma and texture did not show a significant difference in the result. The aroma and texture data obtained on 2.90 (likes) and 2.40 (likes) with 3.5% salt. Keywords: Candied; Garlic; Salt; Soaking; Sugar.
OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE Herawati, Maria Marina; Pudjihartati, Endang; Pramono, Suwijiyo; Sulistyaningsih, Endang; Purwantoro, Aziz
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.490

Abstract

Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.
INDUKSI KALUS DAN PROLIFERASI ARTEMISIA CINA BERG EX POLJAKOV Herawati, Maria Marina; Purwantoro, Aziz; Sulistyaningsih, Endang; Pramono, Suwijiyo
Agric Vol 26 No 1 (2014)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2014.v26.i1.p45-51

Abstract

Penelitian ini bertujuan untuk mengevaluasi efek kondisi terang dan gelap pada induksi dan proliferasi kalus dari kalus Artemisia cina. Eksplan steril akan dikulturkan pada media MS dengan kandungan 2.4-D 1 mg/L dan diletakkan pada kondisi terang dan gelap. Hasil penelitian menunjukkan bahwa induksi kalus akan lebih efektif pada kondisi gelap, sedangkan proliferasi kalus lebih efektif pada kondisi terang. Tahap terbaik untuk proses regenerasi adalah hari ke-48 setelah transfer dari media induksi.
SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov Kristiani, Elizabeth B.E; Kasmiyati, Sri; Herawati, Maria Marina
Agric Vol 27 No 1 (2015)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2015.v27.i1.p30-37

Abstract

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.
KOMPOSISI EKSTRAK STEVIA (STEVIA REBAUDIANA) TERHADAP KARAKTERISTIK SIRUP BIT (BETA VULGARIS L.) Simarmata, Elvi Fitriani; Herawati, Maria Marina; Sutrisno, Alfred Jansen; Handoko, Yoga Aji
Jurnal Penelitian Pertanian Terapan Vol 19, No 3 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i3.1429

Abstract

Beetroots are a type of tubers with a distinctive purplish red color and are beneficial for health with a high amount of betasianin as an antioxidant, as well as its use in preventing infection. But the beetroot is less liked by the public because of the strong enough earthy taste, so it is necessary to do new innovations for the product to be accepted by the community, one of which is the functional beet syrup drink. To create a preferred flavor, the addition of other food additives and sweetener stevia which has a sweetness level of 200-300 times that of sugar and includes non-calorie sweeteners is done. This study aims to analyze the best concentration of stevia extract on the sweetness level and beetroot characteristics and determine the level of panelists' preference for beetroot syrup (Beta vulgaris L.) with organoleptic tests. This research was conducted with a randomized block design (RBD), the data were analyzed by variance (ANOVA), if the data obtained were significantly different, the DMRT would be further tested at 5% level. The results showed that the highest betasianin was in the SBS15 treatment (31.74 mg / l), the highest total dissolved solids was in the SBS treatment (3.25ºBrix), the highest pH was in the SBS15 treatment (4.22), the highest vitamin C was in the SBS15 treatment (11.66 mg ascorbic acid / 10 ml). Organoleptic results showed that beet syrup with the addition of stevia extract of 6% was the most preferred sample by panelists. 
EFFECT OF INDUCED POLYPLOIDY ON PLANT GROWTH, CHLOROPHYLL AND FLAVONOID CONTENT OF ARTEMISIA CINA Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok; Herawati, Maria Marina
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22548

Abstract

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.