Yuni Widyastuti
Balai Besar Penelitian Tanaman Padi, Jl. Raya 9, Sukamandi, Subang, Jawa Barat Telp. (0260) 520157, Faks. (0260) 520158

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PENGARUH PERBEDAAN WAKTU TANAM TETUA PADI HIBRIDA DAN APLIKASI ZAT PENGATUR TUMBUH TERHADAP HASIL BENIH F1 HIPA 8 WAHYUNI, SRI; RUSTIATI, TITA; WIDYASTUTI, YUNI
Agrotrop: Journal on Agriculture Science Vol 3 No 2 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The Effects of Planting Time of TheParental Hybrid Rice and Plant Growth RegulatorApplication on Seed Yield of F1 HIPA 8.Research the effect of planting time of parental lines andapplication of plant growth regulators (GA3and glysine) on seed yield of F1 hybrid rice had beenconducted at The Indonesian Centre of Rice Research on 2010. The objective of the study was to findout combination of plating time and application of plant growth regualtors to get high seed yield ofhybrid rice. Seeds of parental lines Hipa 8 were planting in row ratio 2R:8A at Cilandak, Indramayu onthe dry season of 2010. Treatments to improve seed yield of hybrid rice consisted of (a) planting timeof parental lines (CMS and R), and (b) application of plant growth regualators (GA3 CG1 (commercialgrade) 60 ppm, GA3 CG2 94 ppm, Glysine 40 ppm and control (untreated). Plant growth regualatorwere applied at three times i.e. heading time, 5% plant flowering and 20% plant flowering. Variableevaluated consisted of: plant growth, yield component and seed yield. Result of the exeperiment showedthat tiller number per hill and plant height at vegetatif stage were not affected by treatments. TreatmentA1B2 and A2B2 significantly improved panicle exercition, plant height of restorer and also seed yield.Rice plant treated with A1B2 (planting times of restorer were 12, 15, and 18 days after CMS andapllication GA3 CG1 60 ppm) showed the highest seed yield, followed by A2B2 (planting times ofrestorer were 13, 17 and 21 days after CMS and application of GA3 CG1 60 ppm).
IDENTIFIKASI TOLERANSI KEKERINGAN TETUA PADI HIBRIDA PADA FASE PERKECAMBAHAN MENGGUNAKAN POLIETILEN GLIKOL (PEG) 6000 Widyastuti, Yuni; Purwoko, Bambang Sapta; Yunus, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.324 KB) | DOI: 10.24831/jai.v44i3.13784

Abstract

ABSTRACTDeveloping of  drought tolerant hybrid rice varieties requires parental lines which have tolerance to drought. Polyethylene glycol (PEG) has been widely used as an osmotic solution for detecting drought tolerance at germination stage of rice. The objectives of this experiment were to evaluate variables of drought tolerance at germination stage  and to select drought tolerance of parental lines using osmotic solution PEG 6000 at concentration of 25%. The experiment was conducted at the greenhouse ICABIOGRAD Cimanggu Bogor, during April-May 2014. The design of the experiment was arranged in factorial randomized block design with three replications. The first factor (genotype) had 21 levels and the second factor (osmotic potential) had two levels (0 and 25% concentration levels of PEG 6000). Based on the principal component analysis, six primary indicators were proposed for drought tolerance of rice at germination stage, i.e., germination percentage, seed vigor, seminal root length, seedling length and dry weight of seminal root. Based on the clustering analysis, at 73.76% of similarity rate, the 21 tested genotypes were divided into 2 groups. The genotypes which showed similar responses with Salumpikit were IR 58025B, GMJ 14B, IR 80154B, GMJ 15B, R 3, PK 90, and PK 12 respectively. Keywords: drought tolerance, germination, hybrid rice PEG, parental lines
PERFORMANCE OF PROMISING HYBRID RICE IN TWO DIFFERENT ELEVATIONS OF IRRIGATED LOWLAND IN INDONESIA Widyastuti, Yuni; Satoto, Satoto; Rumanti, I.A.
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The hybrid rice program has been established since early 1990’s at the Indonesia Center for Rice Research (ICRR). Twenty-four experimental hybrid rice varieties which have been developed were tested in lowland rice fields in Sukamandi (West Java) and Batang (Central Java) during the dry season and the rainy season of 2012. Randomized complete block design (RCBD) with three replications was used in each location. The results showed that grains yields were affected by locations, seasons, and genotypes. The genotypes x locations x seasons interaction effect was significant; therefore, the best hybrid was different for each location and season. A7/PK36 hybrid has the best performance in Batang during the dry season, while A7/PK40 and A7/PK32 are the best hybrids in the rainy season. In Sukamandi, nine hybrids were identified as better yielder than that of the check cultivar in the dry season, but not so in the rainy season. Using the correlation and path analysis, we found that the number of panicles per hill and the number of filled grains per panicle could be used as selection criteria for yield in hybrid rice. Keywords: agronomic performance, correlation, hybrid rice, lowland 
YIELD STABILITY OF NEW HYBRID RICE ACROSS LOCATIONS Satoto, Satoto; Rumanti, Indrastuti Apri; Widyastuti, Yuni
AGRIVITA, Journal of Agricultural Science Vol 38, No 1 (2016): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i1.675

Abstract

The adaptation of hybrid rice varieties mostly are in specific location and season, but there are some of the varieties have a wide adaptation then adopted by the farmer in the large area. Replicated yield trials were conducted to study the stability of hybrid rice yield and identify the best location to optimize their yield per ha. We have conducted the trials in three location such as Sukamandi, Salatiga and Malang during two seasons in 2011. We are analyzing data across location and season  using AMMI and Eberhart Russel methods. The AMMI analysis showed that the  IR79156A/PK88 was adaptable to favorable environments but unstable. This hybrid is always performing well and produce the higher yield compare to check variety. Some of other hybrids  were good only in specific location, i.e. IR62829A/BP2280-1E-12-22 and IR58029A/BP2 280-1E-12-22. Those hybrids produced higher yield in Salatiga and Malang, respectively. Seem to AMMI analysis, the result of Eberhart and Russells method also showed that IR79156A/PK81  was the best hybrid with regression slope (b) around 1 with the yield average higher than average of all hybrids. It indicated that this hybrid has a wide adaptation and probably can be cultivated in the wider ecosystem.
Perbedaan Hasil Padi Antarmusim di Lahan Sawah Irigasi Satoto, Satoto; Widyastuti, Yuni; Susanto, Untung; Mejaya, Made Jana
Buletin Iptek Tanaman Pangan Vol 8, No 2 (2013): Desember 2013
Publisher : Puslitbang Tanaman Pangan

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Abstract

Indonesia as a tropical country, has two distinct planting seasons, i.e. rainy (WS, October-April) and dry season (DS, May-September). The difference between the two seasons include the amount of rainfall, air temperature, humidity, solar radiation, and cloudiness which cause yield gap between seasons. Rice yield testing at various locations and seasons using inbred and hybrid varieties showed the occurrence of yield gap patterns. In East Java during the 2001-2003 testing showed inconsistence yield gap between planting seasons across locations. Testing of 29 very early maturing accessions in Kuningan (550 m asl) during DS 2010 and WS 2010/2011 showed no significant yield gap between planting seasons. Similarly, testing of 10 GSR inbred lines along with four check varieties during DS 2012 and WS 2012/2013 showed no significant yield gap due to seasons, genotypes, and plant spacing. Factors which presumably affected the yield gap include air temperature, CO2 concentration, and solar radiation during grain filling period. Increasing temperature had reduced the grain yield, where as increasing CO2 concentration increased plant biomass formation, while optimum solar radiation during grain filling increased grain yield. To reduce yield gap between seasonal planting, the more readily observable causing the yield gap should be anticipated, such as: pests and diseases in each season for each location, assigning suitable variety for specific location and planting season, and application of most appropriate cultivation techniques for each location and season (fertilizers application, plant spacing, irrigation, and pest/diseases management).
Perilaku Pembungaan Galur-galur Tetua Padi Hibrida Widyastuti, Yuni; Rumanti, I. A.; Satoto, Satoto
Buletin Iptek Tanaman Pangan Vol 7, No 2 (2012): Desember 2012
Publisher : Puslitbang Tanaman Pangan

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Abstract

Rice (Oryza sativa L) is naturally a selfpollinated crop, although outcrossing rate of 0.5 to 6.8% had been observed in some genotipes. In hybrid rice breeding, outcrossing ability of parental lines is expected to increase seed set. The successful of hybrid rice seed production is affected by floral characteristics, synchronous of parental line flowering, and other morphological characters which affect pollen transfer from male parent (B or R line) to female parent (A line). Some rice agronomic characters such as number of productive tillers per hill, number of spikelets per panicle, plant height, narrow and short of flag leaf, also panicle exertion, could affect outcrossing rate. The floral characteristics of A line such as: stigma and stylus size, stigma exertion, stigma receptivity period, angle of floret, period of the spikelet opening, and panicle exerted above flag leaf, are also affecting the outcrossing rate. However, the flowering characteristic of male parent (B or R line) such as: anther size, filament length, number of pollen per anther, percentage of exerted anther and duration of spikelet opening, are also contributing to the rate of outcrossing. Study of heritability and genetic variability analysis revealed that all characters supporting outcrossing could be improved by breeding. The environmental factors that affect outcrossing in rice are temperature, relative humidity, light intensity, and wind speed.
PERFORMANCE OF PROMISING HYBRID RICE IN TWO DIFFERENT ELEVATIONS OF IRRIGATED LOWLAND IN INDONESIA Widyastuti, Yuni; Satoto, Satoto; Rumanti, I.A.
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i2.562

Abstract

The hybrid rice program has been established since early 1990?s at the Indonesia Center for Rice Research (ICRR). Twenty-four experimental hybrid rice varieties which have been developed were tested in lowland rice fields in Sukamandi (West Java) and Batang (Central Java) during the dry season and the rainy season of 2012. Randomized complete block design (RCBD) with three replications was used in each location. The results showed that grains yields were affected by locations, seasons, and genotypes. The genotypes x locations x seasons interaction effect was significant; therefore, the best hybrid was different for each location and season. A7/PK36 hybrid has the best performance in Batang during the dry season, while A7/PK40 and A7/PK32 are the best hybrids in the rainy season. In Sukamandi, nine hybrids were identified as better yielder than that of the check cultivar in the dry season, but not so in the rainy season. Using the correlation and path analysis, we found that the number of panicles per hill and the number of filled grains per panicle could be used as selection criteria for yield in hybrid rice.
KORELASI DAN SIDIK LINTAS KARAKTER AGRONOMI PADI HIBRIDA Kartina, Nita; Wibowo, Bayu Pramono; Widyastuti, Yuni; Rumanti, Indrastuti Apri; Satoto, .
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 2 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.951 KB) | DOI: 10.18343/jipi.21.2.76

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Two approachment to increase yield productivity with new variety throught new plant type (NPT) concept and heterosis exploitation of hybrid rice. The research was to evaluated yield and yield component of 36 hybrid rice genotypes in preliminary yield trial at two locations. The experiment was conducted in the second season MT (II) 2013 at the Sukamandi experimentalstation, Subang, West JavaProvince and in Batang, Central Java Province by usingRandomized Complete Block Design (RCBD). The material used were36hybrid rice genotypes and four check varieties namely Hipa8, Hipa Jatim2,Ciherang, and Longping Optima. The results showed that grain yield had been effected by location, genotypes, and both interactions. There were two hybrid rice genotypes that give high yield potential than four check varieties, and one hybrid rice genotype significantly different from Longping Optima. Those hybrids were GMJ6/CRS519 (9.9 t/ha) and GMJ14/CRS757 (9.5 t/ha), and A1/CRS518 (6.2 t/ha). Based on path analysisnumber of productive tiller and number of total grain per panicle have direct effect to yield with path coefficient as 0.4028 and 0.2153. Length panicle and seed set also gave positif and significant direct effect to yield as 0.095 and 0.0956, so these characteristics could be used as selection criteria. 
PENGARUH GA3 DAN BEBERAPA JENIS LARUTAN KIMIA TERHADAP BUNGA DAN KARAKTER MORFOLOGIS TETUA PADI HIBRIDA Widyastuti, Yuni; Wahyuni, Sri; Mulsanti, Indria Wahyu
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 3 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.398 KB) | DOI: 10.18343/jipi.21.3.153

Abstract

The aim of the present study is to optimize the dose of GA3 and substitution chemicals that affect on floral and morphological traits of parental lines for hybrid rice seed production. The experiment was conducted at glass house on field station Sukamandi, Indonesian Center for Rice Research, during April-August 2010. The experimental material comprising both the parental lines of hybrid Hipa 7 i.e. IR58025A and IR40750 were treated with 10 treatments of different kind of GA3, chemicals, and one untreated check was grown in randomized block design with three replications. The results indicated that a liquid GA3 60 ppm, urea 2%, phosphate 1%, and glycine 40 ppm could influence some of the characters favoring outcrossing i.e stigma exertion, panicle exertion, anther and filament length. These chemical (urea 2%, phosphate 1%, and glycine 40 ppm) showed the possibility of the economizing cost of seed production and substitute of GA3
AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli Widyastuti, Yuni; Yuliani, Nia; Widhyastini, I.G.A. Manik
Jurnal Sains Natural Vol 6, No 1 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.015 KB) | DOI: 10.31938/jsn.v6i1.253

Abstract

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, dan   Escherichia coli,   Infusa.