Purnomowati Purnomowati, Purnomowati
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INOKULASI MIKORIZA VESIKULA ARBUSKULA (MVA) CAMPURAN SEBAGAI PENGENDALI PENYAKIT LAYU FUSARIUM PADA TANAMAN SEMANGKA (Citrullus vulgaris Schard) Solihah, Saniyatun Mar’atus; Dwiputranto, Uki; Purnomowati, Purnomowati
Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto Vol 15, No 1 (2013): AGRITECH
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/agritech.v15i1.995

Abstract

A research aimed to examine the effect of dosage interaction and MVA inoculation technique on the incidence of fusarium wilt in watermelon plants has been carried out. Method used was experimental with a Complete Randomized Design (CRD) in a factorial pattern. The first factor was MVA treatment in four levels, i.e. M0 (no MVA given), M1 (MVA = 7.5 g/plant), M2 (MVA = 10 g/plant), M3 (MVA = 12.5 g/plant). Whereas, the second factor was different inoculation techniques: IbJ (VAM was inoculated when seeds were planted) and Ibt (VAM was inoculated when the seedlings were replanted). The main parameter in this research was the incubation period and disease intensity, while the supporting parameters were VAM infection intensity, soil pH, air temperature and relative humidity. Obtained data of the disease intensity and the incubation period were analyzed with analysis of variance (F test) and continued with Least Significant Differences test with the error level of 5%. Results indicated that the interaction of dosages and mixed MVA inoculation technique had suppressed the disease intensity and delayed the incubation period of fusarium wilt in watermelon plants, while the dosages of mixed MVA separately affected the intensity and delayed the incubation period of fusarium wilt in watermelon plants. The mixed MVA dosege of 10 g/plant was the most effective to suppress Fusarium wilt disease intensity in watermelon plants, while the dosage of 12.5 g/plant was the best to delay the incubation period of fusarium wilt in watermelon plants. Key words : inoculation of MVA and Fusarium Wilt Disease
Pertumbuhan Cabai Merah (Capsicum annuum L.) pada Tanah Masam yang Diinokulasi Mikoriza Vesikula Arbuskula (MVA) Campuran dan Pupuk Fosfat Jamilah, Maryam; Purnomowati, Purnomowati; Dwiputranto, Uki
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 1 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.347

Abstract

This research aimed to find out the interaction effect between mixed Vesicular Arbuscular Mycorrhiza (VAM) and phosphate fertilizer to the growth of red chili (C. annuum) in acid soil, and to fnd out the best combination of mixed VAM and phosphate fertilizer to the growth of red chili (C. annuum) in acid soil. This research used an experimental method with Completely Randomized Design (CRD) in a factorial pattern with two factors. The first factor was mixed VAM dosages consisted of four levels: 0; 10; 15; 20 g/plant. The second factor was phosphate fertilizer dosages consisted of four levels:  0; 0,2; 0,4; 0,6 g/plant. Each combination treatment had three replication. The parameters were observed in the form of plant height, stem diameter, plant top dry weight, degree of VAM infection, and P content of plant tissue. Data obtained from the observation was analyzed with Analysis of Variance (ANOVA) at an error rate of 5% and 1%, treatment that showed significant or very significant result, then followed with Honestly Significant Difference (HSD) test. The result showed that interaction between mixed Vesicular Arbuscular Mycorrhiza (VAM) and phosphate fertilizer did not increase the plant height, stem diameter, and plant top dry weight, but each factor increased the plant height, stem diameter, and plant top dry weight. VAM dosage inoculation of 20 g/plant without phosphate fertilizer is the most effective combination in increasing the degree of VAM infection.
Pengaruh Kombinasi Jenis Bahan Pembawa dan Lama Masa Simpan yang Berbeda terhadap Produksi Pelet Biofungisida Trichoderma harzianum Muljowati, Juni Safitri; Purnomowati, Purnomowati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.188

Abstract

This research aimed to know effect of carrier material combination and different storage duration on viability of T. harzianum, also to know the material type and storage duration which yielding highest conidia.  Method used in this research was experimental by using Completely Randomized Design (CRD) with factorial pattern. The first factor was type of carrying material that were white sticky rice meal, mix of 75% white sticky rice meal and 25% mungbean meal, mix of 75% white sticky rice meal and 25% soybean meal, and mix of 75% white sticky rice meal and 25% skim milk that each of such material was inoculated by 108 conidia/ml of T. harzianum with storage duration levels that were 0 weeks, 3 weeks, 6 weeks, and 9 weeks.  Obtained data was analyzed by analysis of variance (F test) with significantly level 95% and 99% and followed by Honestly Significant Difference (HSD).  Result showed that there was interaction between types of carrying material with pellet storage period that showed significant effect on viability of T. harzianum.  Carrying material type of white sticky rice meal with pellet storage period of 9 weeks resulting highest percentage acerrage of conidia seedling (viability) of T. harzianum that was 78,19%.
Pengaruh Pemberian Mikoriza Vesikula Arbuskula (MVA) Campuran terhadap Kemunculan Penyakit Layu Fusarium pada Tanaman Melon (Cucumis melo L.) Farhati, Najmah; Purnomowati, Purnomowati; Dwiputranto, Uki
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.489

Abstract

Melon (Cucumis melo L.) has economic potential to be cultivated because the fruit contains protein, fat, carbohydrate, calcium, phosphor, fiber, iron, vitamin A, vitamin B1, vitamin B2, vitamin C, and niacin. Fusarium wilt caused by Fusarium oxysporum will decrease melon crop production. One of controlling method to Fusarium wilt diseases on melon plants which safe for environtmental by using biological control. One of microorganisms which can be biological control agent is Vesicular Arbuscular Mycorrhiza (VAM). This research use experimental method with a Completely Randomized Design (CRD). The experimental treatment consists of two types of treatment which combine 5 doses of VAM mixture ( 0 g/plant, 10 g/plant, 12,5 g/plant, 15 g/plant, 17,5 g/plant) and two inoculation method VAM is inoculated when seeds are planted and inoculation when the seedlings are replanted. Each treatment was repeated 3 times and each unit consist of three plant, so there are 30 units of experiments or 90 plants. The main variabels are observed consist of the incubation periode of the disease and the intensity of fusarium wilt and the supporting variabels consist of pH, temperature, humidity, and the scale of infection. The mixed MVA 15 g/plant dosage inoculated when seeds are planted and 15 g/plant dosage inoculated when the seedlings are replanted is the most effective to suppress incubation period of Fusarium wilt disease.
Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium Mumpuni, Aris; Ekowati, Nuraeni; Purnomowati, Purnomowati; Purwati, Endang Sri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11660

Abstract

Pleurotus ostreatus cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of P. ostreatus on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating P. ostreatus in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.
Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium Ratnaningtyas, Nuniek Ina; Purnomowati, Purnomowati; Purwati, Endang Sri; Septiana, Aisyah Tri; Ekowati, Nuraeni; Supriyadi, Adi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11512

Abstract

Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than  from ethanolic extract (581.80 < 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 < 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.
PENYAKIT PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.) YANG DISEBABKAN OLEH CENDAWAN DI PERTANAMAN RAKYAT DAERAH BREBES Widiyarti, Eka; Purnomowati, Purnomowati; Sucianto, Eddy Tri
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.3.552

Abstract

Shallot (Allium ascalonicum L.) is one of the horticultural commodities which highly developed and cultivated in Brebes. Brebes contributed to fulfill up to 23% to the national needs. But, its production decreased 0.87% annually. The intensification of shallot production faced many problems, especially the diseases caused by the pathogenic fungi causing the low crop production. We conducted this study during the period of May–October 2012. The purpose of this study was to find out the type of pathogenic fungi and to determine the diseases including the dominant disease on shallot crops in Brebes. The research was a survey, and a diagonally purposive sampling technique applied at five different locations. We identified the plant diseases based on identification key books of the Penyakit-Penyakit Tanaman Hortikultura and the Plant Disease Handbook as references.  We described the diseases according to the signs and symptoms followed by the isolation of pathogenic fungi that cause disease. The pathogenic fungi identification was completed using the Illustrated Genera of Imperfect Fungi,  the Compedium of Soil Fungi, and the Pengenalan Kapang Tropik Umum as references. This study found three diseases in shallot crops, i.e., Fusarium tuber rot, Aspergillus tuber rot and Curvularia leaf spot. The dominant shallot crop disease was the Curvularia leaf spot.
PENGARUH INOKULASI MIKORIZA VESIKULA ARBUSKULA (MVA) CAMPURAN TERHADAP KEMUNCULAN PENYAKIT LAYU FUSARIUM PADA TANAMAN TOMAT (Solanum lycopersicum) Hasanah, Uswatun; Purnomowati, Purnomowati; Dwiputranto, Uki
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2017.4.1.382

Abstract

Tomato has an important role to fullfil the nutrition of society. The most important problem in the cultivation of tomatoes is fusarium wilt caused by Fusarium oxysporum it will attacking the plants from nursery to adult. One of the alternative control is use the Vesicles Arbuscular Mycorrhizae (VAM). The success of VAM infection in plants is determined by the dose and the inoculation. The aim of this research is to determine the effect of dose and mixture VAM inoculation to against the emergence of fusarium wilt in tomato plants and to determine the dosage mixture VAM inoculation as the most effective way for controlling fusarium wilt in tomato plants. The method of this research used experimental with completely randomized design. The experimental treatment consists of two types of treatment that are combined with used 5 doses of VAM mixture (0 g/plant, 10 g/plant, 12,5 g/plant, 15 g/plant, 17,5 g/plant) and used two ways of inoculation ( inoculation when the seed is planted and inoculation when transplanting the seeds). Each treatment was repeated 3 times and each test are three plants. The parameters was observed the incubation period of the disease and the intensity of fusarium wilt as the main parameter and the measurement of pH, temperature, humidity room, and the degree of infection as supporting parameters. The results of this research showed that the dosage and inoculation of VAM mixture is not able to reduce the emergence of fusarium wilt on tomatoes, but it was able to extend the incubation period of fusarium wilt on tomato plants a dose with 10 g /plant inoculated plants when the seeds are planted and inoculation when transplanting the seeds.
KAJIAN PENYAKIT YANG DISEBABKAN OLEH CENDAWAN PADA TANAMAN CABAI MERAH (Capsicum annum L.) DI PERTANAMAN RAKYAT KABUPATEN BREBES Suwardani, Nita Wahyu; Purnomowati, Purnomowati; Sucianto, Eddy Tri
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.3.554

Abstract

This research aimed to determine the various diseases caused by fungi, to identify the fungi causing the disease, and to determine the most dominant disease in red chili crops (Capsicum annum L.) from smallholder agriculture located in Kaligiri Sirampog Brebes. The research was a survey applying purposive random sampling technique. This study collected samples from five locations, within five plots placed diagonally at each site. This study picked ten infected plants from each plot, identified the disease; isolated and identified the fungi causing the disease. This research also measured the environmental factors such as temperature, humidity, and soil pH. The results showed diseases in red chili plants (Capsicum annum L.) were the leaf spot caused by Cercospora sp., the anthracnose caused by Colletotrichum sp., and the fusarium wilt caused by Fusarium sp., and the most dominant were leaf spot disease.