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OPTIMALISASI TEKNIK EKSTRAKSI DAN ISOLASI DNA TANAMAN SUREN (TOONA SURENI MERR.) UNTUK ANALISIS KERAGAMAN GENETIK BERDASARKAN RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) Restu, Muh; Mukrimin, Mukrimin; Gusmiaty, Gusmiaty
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.65 KB) | DOI: 10.31258/jnat.14.1.138-142

Abstract

The species of trees have different secondary compounds that need optimum extraction techniques. Appropriate extraction techniquesdetermine the quality and quantity of DNA produced. This research aims to found optimal of extraction methods and DNA isolation, thento created genome DNA in high quality and quantity, so that it can be using for genetic variation analyses in Suren (Toona sureni Merr.) byRandom Amplified Polymorphic DNA (RAPD). This study shows that DNA concentrates were 763.3 ?g/ml, 180.0 ?g/ml, 383.3 ?g/ml, and436.7 ?g/ml. While based on the results of PCR amplification using the primers OPD 03 shows that the four extraction methods used, the extraction method of number 3 has been able to produce genomic DNA with better quality and more number of bands, although the quantityis lower.
POLIMORFISME PENANDA RAPD UNTUK ANALISIS KERAGAMAN GENETIK PINUSMERKUSII DI HUTAN PENDIDIKANUNHAS Gusmiaty, Gusmiaty; Restu, Muh.; Asrianny, Asrianny; Larekeng, Siti Halimah
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.822 KB) | DOI: 10.31258/jnat.16.2.47-53

Abstract

The aims of Genetic study of pinus identified stand in Unhas Experimental Forest is to analyses of genetic characteristics of stand, based on RAPD (Random Amplified Polymorphic DNA) marker. The study was conducted in Biotechnology and Tree Breeding Laboratory, Forestry Faculty, Hasanuddin University. The method are DNA isolation, Primers selection and RAPD analyses. DNA analyses of Pine with ten RAPD marker showed number of alel varietied and polymorphic. Coefficient of similarity in population have number of 0.15-0.73.Highest genetic distance is 0.9630 and lowest genetic distance is 0.2698. Number of genetic diversity of Pine in Experimental Forest Hasanuddin University is 0,489 and categorized highly.
POLYMORPHISM OF SIMPLE SEQUENCE REPEAT REGIONS OF SULAWESI EBONY (DIOSPHYROS CELEBICA BAKH.) IN EXPERIMENTAL FOREST OF HASANUDDIN UNIVERSITY PROVENANCE Larekeng, Siti Halimah; Restu, Muh.; Gusmiaty, Gusmiaty; Rismawati, Rismawati
AgroTech Journal Vol 1, No 1 (2016): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2168.934 KB) | DOI: 10.31327/atj.v1i1.173

Abstract

Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsatellite marker of Ebenaceae Family for amplifying Ebony DNA, and to determine the appropriate PCR annealing temperatures. The DNA isolation of Ebony leaves from Experimental Forest of Hasanuddin University Provenance was carried out using Genomic DNA Mini Kit (Plant) Geneaid protocol. Nine of seventeen selected primers from the Genus Diospyros were able to amplify Ebony DNA. Amplification products produced polymorphic bands with different annealing temperatures (ranged from 53 to 56°C). These nine polymorphic primers will be recommended to use for future studies in genetic diversity as well as pollen dispersal pattern analyses.
RESPON KINETIN DAN TIPE EKSPLAN JABON MERAH (Antocephalus macrophyllus (Roxb.) Havil) SECARA IN VITRO Putriana, Putriana; Gusmiaty, Gusmiaty; M, Restu; Musriati, Musriati; N, Aida
BIOMA : JURNAL BIOLOGI MAKASSAR Vol 4, No 1 (2019)
Publisher : Departemen Biologi, Fakultas MIPA, UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v4i1.6363

Abstract

Keberhasilan kultur jaringan dipengaruhi oleh jenis atau tipe eksplan dan penambahan zat pengatur tumbuh. Kinetin (6-furfurylaminopurine) merupakan zat pengatur tumbuh golongan sitokinin yang banyak digunakan dalam kultur jaringan. Penelitian ini bertujuan mengetahui pengaruh penambahan kinetin dan tipe eksplan terhadap pembiakan in vitro jabon merah. Hasil penelitian menunjukkan bahwa kinetin 7 ppm memberikan pengaruh terbaik terhadap pertumbuhan daun dan akar. Tipe eksplan bagian pucuk merupakan bagian terbaik digunakan untuk pembiakan in vitro. Keyword : Antocephalus macrophyllus (Roxb.) Havil, kinetin, in vitro, tipe eksplan
Identifikasi dan Karakterisasi Mikoriza pada Tegakan Nyatoh (Palaquium sp.) Kurnia, Kurnia; Gusmiaty, Gusmiaty; Larekeng, Siti Halimah
PERENNIAL Vol 15, No 1 (2019)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v15i1.6850

Abstract

This research aimed to identify the mycorrhiza types that associated in roots and soil on  Palaquium sp. plant. This  research has implemented on December 2017 until February 2018. This research procedures made by two stages includes collecting soil and root samples on Palaquium sp plant on  Ko'mara Community Forest, Takalar District. Therefore, the second step is isolation, identification, and observing colonization of mycorrhiza spores in the Integrated Laboratory and Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. The results showed types of spore that found were Glomus, Gigaspora and Acaulospora. Glomus spore has highest relative abundance in root samples whereas on soil samples were Glomus, Gigaspora, and Schleroderma with Glomus spores has highest relative abundance in. The colonization percentage on site was intermediate.
Eksplorasi Cendawan Rhizosfer Pada Tegakan Hutan Rakyat suren Untuk Meningkatkan Pertumbuhan Tanaman Rina, Payangan Yanti; Gusmiaty, Gusmiaty; Restu, Muh
BIOMA : JURNAL BIOLOGI MAKASSAR Vol 4, No 2 (2019)
Publisher : Departemen Biologi, Fakultas MIPA, UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v4i2.8397

Abstract

Pertumbuhan tanaman dapat dipengaruhi oleh adanya keragaman mikroba yang yang terdapat di rhizosfer, salah satunya yaitu cendawan. Cendawan rhizosfer yang terdapat pada akar tanaman dapat meningkatkan pertumbuhan tanaman dan perlindungan terhadap mikroba tertentu. Penelitian ini bertujuan untuk mengidentifikasi cendawan rhizosfer. Metode penelitian yang digunakan yaitu isolasi cendawan menggunakan teknik pengenceran 10-2 dan 10-3 , serta identifikasi cendawan berdasarkan karakter morfologi. Hasil isolasi cendawan rhizosfer diperoleh sebanyak 33 isolat yang terdiri atas 5 genus yaitu Aspergillus, Trichoderma, Rhizopus, Penicillium dan Fusarium.Kata Kunci : Suren, Rhizosfer, Cendawan