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Identification of excretory secretory (ES) liquid antigen protein Fasciola gigantica with polyethilene glycol (PEG) separation Junaidi, Yendri; Malawati, Ima; Sriasih, Made
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (824.988 KB) | DOI: 10.22146/ijbiotech.27017

Abstract

Fasciola gigantica diagnosis usually performed by detection of worm eggs presence in the feces,but this conventional method has many disadvantages. Early diagnosis (early detection) cannot be performed in conventional methods because the worms in the hosts body began to lay eggs at the age of 8–12 weeks of patency. The current detection method that is based on antibody­antigen reactions using excreted/secreted (ES) liquid by adult F. gigantica, is believed to be used for the early detection of fasciolosis. This study aimed to characterize the antigenic components of F. gigantica extretory/secretory products that could be used as a vaccine candidate development for early fasciolosis diagnostics. ES products were separated by PEG4000 at various concentrations (8%, 16%, 24%), then precipitates (pellets) obtained were dialyzed and characterized using SDS­PAGE and Western blotting. Results from SDSPAGE showed that there were 18 proteins bands with 7–70 kDa molecular weights. Western blotting on pellets derived from PEG separation at various concentrations affirmed that the proteins of 50, 25 and 20 kDa were antigenic at 8% PEG concentration, the 25 kDa and 50 kDa were antigenic at 16% PEG concentrations and the 25 kDa was antigenic at 25% PEG concentration
GASTROINTESTINAL PARASITE INFECTION ON BALI CATTLE RAISED IN SEMI-INTENSIVE FARMING SYSTEM IN DOMPU, SUMBAWA ISLAND: A PRELIMINARY STUDY Sriasih, Made; Yanuarianto, Oscar; Dahlanuddin, Dahlanuddin; Pomroy, William Ernest
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2018.v06.i01.p01

Abstract

To date, there are very few studies have been carried out on the effect of parasite infection on Bali cattle, and more importantly, there is no published data on the epidemiology of worm infections on Bali cattle in the island of Sumbawa, Indonesia. This research aimed to undertake a preliminary study on gastrointestinal (GI) parasitic infection on Bali cattle raised under semi-intensive farming system in five different sites in district of Dompu, Sumbawa island. Fecal samples of 76 Bali cattle, from three farmer groups namely site number 1, 4 and 5, were collected, processed and examined under a microscope, with 100x magnification, for egg fecal counting or oocyst fecal counting using the modified McMaster technique. The number of eggs/oocysts observed was then determined and identified. The results show that the eggs/oocysts ranged from 50-14800. The types of GI parasites identified were Strongyloides sp, Strongyle sp, Toxocara sp, Trichuris sp, and Coccidia. Coccidia oocyst is a type of parasite which was found with the highest percentage of 23.68% followed by Strongyle sp. (18.42%) and Toxocara sp. (10.52%). The overall prevalence of GI parasitic infection in cattle accounted for 43.42%. Young calves (0-3 months old) was more susceptible to GI parasitic infection among other age groups. This preliminary data could be used as a basic data on gastrointestinal parasitic infection in Dompu district. These results may also indicate the need to develop control strategies aiming at lowering the gastrointestinal parasitism.
Identifikasi Keragaman Gen Leptin pada sapi Bali dan kambing Kacang Syarifulaya, Nining; Sriasih, Made; Maskur, Maskur
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 1 No 1 (2015): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v1i1.9

Abstract

The purpose of this research was to identify polymorphism of leptin gene in Bali cattle and Kacang goat by PCR-RFLP technique. Material used was blood samples collected from 50 head of Bali cattle and 50 head of Kacang goat. Identification procedure was consisted of three stages namely extraction of DNA genom, PCR amplication of leptin gene (552bp on intron 2-exon 3) and RFLP method using enzyme  restriction (BsaA1). Primer sequences used were forward primer: 5'GTCTGGAGGCA AAGGGCAGAGT-3'and reverse primer: 5'CCACCACCTCTGTGGAGTAG-3'. Data analyzed were frequency of allele, genotype frequency, heterozygosity value, and value of Polymorphic Informative Content (PIC). The results of this study indicate that three genotypes (AA, AG and GG) were found on Bali cattle whilst only two genotypes (AA andAG) were on Kacang goat. The values of heterozygosity observation (Ho) and expectation heterozygosity (He) on Bali cattle were 0.08 and 0.48 respectively. Kacang goat showed the value of 0,14 (Ho) and 0.14 (He). The PIC value of Leptin-BsaA1gene on Bali cattle was 0.43 (moderate high), and on Kacang goat considered as low (0.13). These results showed than Leptin-BsaA1 in Bali cattle and Kacang goat may be used as a genetic marker for selection.
POTENSI AIR LIUR SEBAGAI PERANTARA DALAM PEMERIKSAAN NONINVASIVE PADA HEWAN PIARAAN (POTENTIAL OF SALIVA AS A MEDIATOR FOR THE NONINVASIVE EXAMINATION OF DOMESTIC ANIMALS) Depamede, Sulaiman Ngongu; Rosyidi, Anwar; Sriasih, Made; ., Dahlanuddin; Yulianti, Enny; ., Suparman
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Saliva as unique biological components of the oral cavity has potential as a mediator for noninvasivebiological test. For future livestock industry the application of noninvasive test is strongly necessary dueto animal welfare porpuse. Until now the research and development of the using saliva as a mediator forinvasive test is predominant for humans, while for livestock or domestic animals is still limited. Techniquesand methods that are commonly used are related to proteomics analysis. With this method a lot of thingsthat can be brought about proteomics of saliva are beneficial to the development of saliva-based biomarkers.Although this method is expensive, researches on the use of saliva in the field of livestock industries areurgently needed. It is expected that noninvasive biological test methods based on saliva as a mediator canbe performed immediately.
STUDI PENDAHULUAN CEMARAN AIR LIMBAH RUMAH POTONG HEWAN DI KOTA MATARAM Aini, Aini; Sriasih, Made; Kisworo, Djoko
Jurnal Ilmu Lingkungan Vol 15, No 1 (2017): April 2017
Publisher : Program Studi Ilmu Lingkungan,Program Pascasarjana, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.078 KB) | DOI: 10.14710/jil.15.1.42-48

Abstract

ABSTRAKTujuan penelitian ini adalah untuk mengetahui cemaran baku mutu air limbah Rumah Potong Hewan (RPH) Sapi dan Ayam di Kota Mataram. Semua hasil melebihi baku mutu kecuali nilai pH. Kandungan ALT rerata air limbah RPH sapi 284004 x 104 koloni/ml dan mengandung mikroba jenis E.coli dan Salmonella. Sedangkan air limbah RPH ayam ALT rerata 101596 x 105 koloni/ml dan mengandung kuman jenis E. coli, Pseudomonas aeruginosa dan Klebsiella pneumoniae. Tingginya kandungan BOD, COD, TSS, ammonia, pH, minyak dan lemak maupun cemaran mikroba diduga disebabkan tidak difungsikannya Instalasi Pengolahan Limbah (IPAL) pada RPH sapi dan tidak optimalnya fungsi IPAL di RPH ayam.Kata kunci: RPH sapi, RPH ayam, Baku mutu limbah RPH, Cemaran mikroba, kota Mataram yABSTRACTThe purpose of this study was to determine the contamination of waste water quality standard and microbiologic contamination ofcattle and chicken slaughterhousein Mataram city. TPC average content of waste water of cattle slaughterhouse was 284004 x 104 colonies/ ml, and contained microbial species of E. coli and Salmonella. However, the TPC avarange of wastewater of chicken slaughterhouse accounted for 101596 x 105 colonies/ml and contained microbial species of E. coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. High content of BOD, COD, TSS, ammonia, pH, oil and fats as well as microbial contamination may relate to disfunctionality of Waste Water Treatment Installation (IPAL) in cattle slaughterhouse and too less optimum function of the IPAL in chicken slaughterhouse.Keywords: cattle slaughter house, chicken slaughter house, slaughterhouse waste quality standard, microbial contamination, Mataram cityCitation: Aini, A., Sriasih, M, dan Kisworo, D. (2017). Studi Pendahuluan Cemaran Air Limbah Rumah Potong Hewan di Kota Mataram. Jurnal Ilmu Lingkungan, 15(1), 42-48, doi:10.14710/jil.15.1.42-48
Identifikasi Keragaman Genetik Gen Growth Hormon Receptor Dengan Enzim Restriksi Mbo Ii (Ghr|Mbo Ii) pada Sapi Bali Huda, Nurul; Sriasih, Made; Maskur, Maskur
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 1 No 1 (2015): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v1i1.7

Abstract

Growth Hormone Receptor (GHR) gene is a member of the super familycytokine/hematopoietin receptor. GHR plays a role in mediating the biological activity of growth hormone on target cells that lead to the affection of the nature ofgrowth in beef cattle carcasses and the nature of milk production in dairy cows. This study was designed to identify the genetic diversity of GHR genes exon 10 in Bali cattle. Genotype variation of gene candidates was identified using PCR-RFLP technique. The results showed that mutation at position of 755bp exon 10 of GHR gene as determined by Maskur (2012) can be identified using MBO II restriction enzymes at position of 125bp or at position of 282bp base nucleotidescaused by transition mutation of  C/T and substitution of amino acid threonine to isoleucine.Genotype identification of  GHR gene exon 10 resulted in three individual genotypes in Bali cattle population namely CC, TT and CT with frequency of 0.1931; 0.5455 and 0.2614respectively. The result of X² test and heterozygosity observationas well as heterozygosity expectation indicate that the genotypedistribution on Bali cattle population in this study were not in Hardy-Weinberg equilibrium (Hardy-Weinberg Equilibrium/ HWE). The value of  GHR|MBO II gene Polymorphic Informative Content was 0.342051, might be used for genetic identifier, and was quite informative as gene identifier for linkage analysis in the population.
Improvements of PCR Amplification of Guanine plus Cytosine-Rich Constructs of Mycobacterium tuberculosis Gene using DMSO Sabrina, Yunita; Lestarini, Ima Arum; Ekawanti, Ardiana; Sriasih, Made; Depamede, Sulaiman N.; Ali, Muhamad
Jurnal Kedokteran Vol 1 No 1 (2012)
Publisher : Faculty of Medicine Universitas Mataram

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Abstract

Vaccine research entered a new era when several useful molecular research tools were established. Instead of attenuated virulent microorganisms or killed virulent microorganisms, effective subunit vaccines were developed using recombinant DNA technology. By using the technology, selected genes of the virulent microorganisms can be amplified, cloned, expressed, and evaluated as vaccine components in challenge studies. However, a major bottleneck with the amplification of functional genes from Mycobacterium tubeculosis containing guanine plus cytosin-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. To solve this problem in this research, the amplification reaction was modified by addition of dimethyl sulfoxide (DMSO) into amplification reaction mixtures. It was found that 10% (v/v) of DMSO in the reaction mixture improved the amplification of GC-rich template of M. tuberculosis gene. This result indicating that amplification of unbalanced content of G and C deoxyribonucleotides genome could be improved using low-cost organic molecule, DMSO. Therefore, the DMSO should be widely useful as an enhancer to improve the amplification of GC rich construct from other genome. Keywords: Mycobacterium tubeculosis,vaccine, dimethyl sulfoxide, Guanine-Cytosine
Analisis Tingkat Pengetahuan, Sikap dan Perilaku Peternak Unggas Terhadap Upaya Pencegahan Penyakit Flu Burung Effendi, Rusman; Dradjat, Adji Santoso; Sriasih, Made
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v2i1.21

Abstract

The aim of this research was to describe and to correlate knowledge, attitude and practice (KAP) of thepoultry breeders in preventing Avian Influenza (AI). This research used face-to-face interview, cross sectionaldesign study with a questionnaire for 100 respondentsand data were analyzed with Rho Spearman. The resultshowed that 82% of respondents washed their hands with soap after handling poultry but only 5% ofrespondents used mask when contacting with poultry. Chickens were kept by respondents most. The cagelocation near to the house accounted for 71%, most of respondents cleaned the cage routinely but there were11% of respondents who did not keep their poultry into the cage. All respondents revealed that the poultrywere not vaccinated periodically. It was about 37.3% of respondents burned dead poultry, and 55.2% and 22.4% buried and throw away to the river respectively. They disagreed with stamping out accounted for 48% andonly 16% of respondents agreed to sell their unhealthy poultry. No respondents informed to the authority ofsub-village when suddent death of the chickens was found. Respondent?s knowledge, attitude and practicewere suffient in preventing Avian Influenza and there was significant correlation between knowledge, attitudeand practice (P<0,05).