Rini Susilowati, Rini
Balai Besar Penelitian dan Pengembangan Pengolahan Produk dan Bioteknologi, Badan Penelitian dan Pengembangan Kelautan Perikanan Jln. Petamburan VI, Slipi Jakarta Pusat 10260 Indonesia

Published : 18 Documents

Found 18 Documents

Kandungan Nutrisi, Aktivitas Penghambatan ACE dan Antioksidan Hemibagrus nemurus Asal Waduk Cirata, Jawa Barat, Indonesia Susilowati, Rini; Fithriani, Diini; Sugiyono, Sugiyono
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): Desember 2017
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v12i2.355


AbstrakKandungan nutrisi dan aktivitas biologis ikan baung (Hemibagrus nemurus) ditentukan oleh kondisi lingkungan dan ketersediaan sumber makanannya. Ikan baung yang dikonsumsi oleh masyarakat sebagai sumber protein dapat ditemukan di habitat aslinya dan lingkungan budidaya dalam karamba jaring apung. Penelitian ini bertujuan untuk menentukan kandungan nutrisi dan aktivitas biologis ikan baung asal Waduk Cirata sebagai penghambat enzim pengubah angiotensin (ACE inhibitor) dan antioksidan. Hasil penelitian menunjukkan bahwa kadar protein dan kadar abu ikan baung hasil budidaya maupun alam tidak berbeda nyata, sedangkan kadar lemak ikan baung hasil tangkapan alam jauh lebih tinggi dibanding kadar lemak hasil budidaya. Kadar mineral makro dan mikro menunjukkan perbedaan yang signifikan pada kadar K (budidaya 313,04±14,84 mg/100g; alam 457,33±7,50 mg/100g) dan Ca (budidaya 29,15±1,06 mg/100g; alam 42,13±0,85 mg/100g). Mineral mikro didominasi oleh Zn sebesar 0,42±0,04 mg/100g (budidaya) dan 0,44±0,02 mg/100g (alam). Asam amino esensial (AAE) yang dominan yaitu lisin sebesar 7,70±0,97 mg/g (budidaya) dan fenilalanin sebesar 0,80±0,26 mg/g (alam), sedangkan asam amino non esensial (AANE) yang utama pada populasi budidaya adalah alo iso-leusin sebesar 13,77±0,23 mg/g dan prolin 1,79±0,70 mg/g (alam). Rasio AAE/AANE populasi budidaya memiliki nilai sebesar 0,83 dan alam sebesar 0,54. Aktivitas biologi ikan baung menunjukkan nilai aktivitas antioksidan pada populasi budidaya sebesar 0,16±0,02 µmol Fe2+/g dan alam sebesar 0,09 µmol Fe2+/g berat kering. Sedangkan aktivitas penghambat  ACE menunjukkan nilai penghambatan pada populasi budidaya sebesar 96,18±1.37% dan alam  sebesar 88,76±1,82%. Secara umum, ikan baung dari Waduk Cirata memiliki kandungan gizi dan komposisi kimia yang bagus, begitu juga dengan bioaktivitasnya sebagai penghambat ACE, sehingga berpotensi sebagai bahan baku suplemen kesehatan, khususnya untuk suplemen penghambat ACE. Nutritional Contents, ACE Inhibitor and Antioxidant Activities of  Hemibagrus nemurusfrom Cirata Reservoir, West Java, IndonesiaAbstractThe nutritional content and biological activities of redtail catfish (Hemibagrus  nemurus) are determined by environmental condition and food sources. Redtail catfish is consumed by peoples as protein source. It can be found in their natural habitat as well as in maricultured-cage system. This study was carried out to determine the nutritional content and biological activity of redtail catfis h from Cirata Reservoir as ACE and antioxidant inhibitor. The result showed the that protein and ash contents of both populations were not significantly different, while fat content of wild population  was much higher than that of cultured. The Mineral content showed  significant difference for K content (cultured 313.04±14.84 mg/100g; wild 457.33±7.50 mg/100g) and Ca content (cultured 29.15±1.06mg/100g; wild 42.13±0.85 mg/100g). The micro minerals were dominated by Zn i.e., 0.42±0.04 mg/100 for cultured and 0.44±0.02 mg/100g for wild fish. The essential amino acids (EAA) were dominated by lysine i.e., 7.70±0.97 for cultured and phenylalanine i.e., 0.80±0.26 mg/g for wild; while the major non-essential amino acids (AANE) were alo iso-leoucine i.e., 13.77±0.23 mg/g (cultured) and proline i.e., 1.79±0.70 mg/g (wild). The EAA/AANE ratio showed that the cultured population was 0.83 and wild was 0.54. The biological activity of redtail catfish showed that the cultured population had antioxidants activity value of 0.16±0.02 µmol Fe2+/g while the wild was 0,09 µmol Fe2+/g. The inhibitory activity of angiotensin in converting enzyme (ACE inhibitory) of wild population was 84.41±1.17% and cultured was 88.76±1.82%. In general redtail catfish from Cirata Reservoir had a complete nutrient content and chemical composition, as well as its bioactivity as ACE inhibitor. So that the fish is very potent for raw material of health supplement, especially for ACE inhibitor supplement
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 4 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.388 KB) | DOI: 10.14710/ik.ijms.24.4.164-170


Excessive human waste nutrients, which usually consist of nitrogenous and phosphate compounds, are known as the major environmental stresses in coastal waters. Therefore, monitoring of nutrients level is very important in marine reserve park. This study presents an application of multi-metric index in accessing the spatial and temporal level of a nutrient in surface water at Batam City Marine Reserve Park, Indonesia. Research had been done with seasonal and spatial zones on three major islands namely Petong, Abang, and Dedep. Water samples from each station were taken from the surface water. Phosphate, nitrate, nitrite, and ammonia, were analyzed in situ and chlorophyll was conducted in the laboratory by spectrophotometric method. Statistical multi-metric detected the average eutrophication index (EI) value between 0.2-0.7, which is mesotrophic to eutrophic. Petong and Abang zones were categorized as eutrophic, and Dadap was mesotrophic. Continuous nutrients contamination throughout seasonal may be derived from sedimentation and domestic run-off from human-populated islands in the middle and northern region. Therefore, ecosystem rehabilitation and mitigation of anthropogenic run-off are needed to optimise the conservation management. Moreover, Dadap zone as the area with the least anthropogenic pressures is potential to be the core of conservation area in Batam City marine reserve park.
Biodiesel production from microalgae Botryococcus braunii sriamini, Sriamini; Susilowati, Rini
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 1 (2010): May 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i1.43


Increasing energy needs cause diminishing energy resources. This encourages the searchfor renewable energy sources to anticipate scarcity. One of the new energy source is microalgae.Microalgae have a high variation of species and have a great potential to be developed as foodand other chemical products. Microalgae has been developed as a potential source of biodieselto replace petroleum fuels derived from foss ils. Of several microalgae s pecies studied,Botryococcus braunii produces the largest oil content, i.e. 75% dry weight. This paper describessteps of producing oil from B. brauniiwhich includes preparation of microalgae biomass,biomass harvesting, and extraction of oil. Oil content of B. braunii is composed mostly ofhydrocarbons (± 15–76% by dry weight), called botryococcene. This type of hydrocarbon ispotential as an energy source of biodiesel.
Chemical Composition and Fatty Acid Profile of Some Indonesian Sea Cucumbers Fawzya, Yusro Nuri; Januar, Hedi Indra; Susilowati, Rini; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.118


Indonesia has a great sea cucumber resource which is useful as functional food. Even though much information about the biopotency of sea cucumbers have been published, however, studies on nutrition value of Indonesian sea cucumbers are still beneficial due to the great diversity and uniqueness of each species. The present work was  intended to obtain information about chemical composition and fatty acid profile of 4 (four) types of sea cucumbers which are frequently found in Halmahera water, North Maluku (Molucca),  one  of  sea  cucumbers  producing area  in Indonesia. The samples coded as  H-03, H-04, H-05 and H-10, were identified as Bohadschia  argus, Holothuria  fuscogilva, Thelenota  ananas, and Actinophyga lecanora. All sea cucumbers showed high protein content, more than 60% (drybase/db), except for T. ananas which had the lowest protein content (48.26% db). As for fats, T. ananas showed the highest amount (2.35%db) with dominated saturated fatty acids (SFA). On the other side, A. lecanora showed higher value of PUFA compared to SFA and MUFA.  Palmitic acid (C16:0) was the most abundant SFA in the most samples with the amount of 0.49–4.9 mg/g sample. Among PUFA, eicosapentaenoic acid/EPA (C20:5n3) was detected, and eicosatrienoic acid (C20:3n6) was relatively higher than the others. The ratio of w6/w3 fatty acid was 1.2–3.2 showing that total w6 fatty acid was higher than total w3 fatty acid. However, this value is safe enough to protect against chronic and degenerative diseases.
Composition of Fatty Acids in Evaluation of Sea Cucumber Potency for Nutraceutical Product Development Susilowati, Rini; Pratitis, Asri; Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.238


Unsaturated fatty acid in food has an effect to improve human health, while saturated fatty acid has the opposite impact. Ratio between both types of fatty acids is important to evaluate the biological material for nutraceutical product development. This paper aimed to evaluate the nutraceutical potency of sea cucumber from Kendari Bay Waters, South East Sulawesi, based on their fatty acids composition. The analysis was conducted by the 1H-NMR (Hidrogen-Nuclear Magnetic Resonance) method. Several species within 4 genera (Holothuria, Bohadschia, Actinopyga, and Stichopus) were selected as the subject of the study. Comparison of fatty acid composition has been done in genera level, the preliminary study found that variation between species among the same genera was insignificant (p >0.05). Results of the study detected that Holothuria contained with an even ratio of Poly Unsaturated Fatty Acids (PUFA) and Saturated Fatty Acids (SFA), but Actinopyga was detected with low PUFA/SFA ratio. It may suggest that Holothuria from this region is the most potential raw material for nutraceutical product development. Meanwhile, precaution is needed for genera that contained with low PUFA/SFA ratio. Furthermore, comparison with other studies detected that fatty acids composition is not morphological specific, but vary, according to food viability, environmental and geographic location. Therefore, preliminary screening is important in evaluation of sea cucumber material for nutraceutical product development.  
Nutritional and Albumin Content of Swamp Fishes from Merauke, Papua, Indonesia Susilowati, Rini; Sugiyono, Sugiyono; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.268


 Study on chemical content of swamp fishes from Merauke has been conducted to obtain nutritional status  of these fishes. Sampling was conducted  twice (August and November 2015) in Merauke. There were six dominant fish species, namely striped snakehead (Channa striata), nile tilapia (Oreochromis niloticus), tade gray mullet (Liza tade), philippine catfish (Clarias batrachus), barramundi (Lates calcarifer), and climbing perch (Anabas testudineus). Result on proximate analysis showed that the fishes had moisture content of 75.73±0.25%-81.45±0.04%, ash 0.94±0.01%-1.26±0.21%, protein 17.11±0.09%-18.92±0.05%, and lipid 0.59±0.19%-3.80±0.63%. The dominant essential amino acid of 6 swamp fishes from Merauke was lysine followed by leucine. Meanwhile the dominant non-essential amino acid was glutamic acid (32.26+0.61 mg/g), followed by aspartic acid. Calcium (Ca) was the dominant macro mineral for all fishes studied, being the highest was in nile tilapia (328.76±8.14 mg/100 g). Micro mineral was dominated by the presence of Selenium (Se) which the highest was in nile tilapia (0.084±0.005 mg/100g). These fishes were also rich in albumin, being the highest was stripe snakehead (138.59±1.68 mg/g). The average of total fatty acid showed that the fishes had saturated fatty acid (SFA) content of 46.30±0.27%, monosaturated fatty acid (MUFA) 9.20±1.6%  and polyunsaturate fatty acid (PUFA) 34.49±0.44% of total fatty acid. From the result, it can be concluded that fish harvested from swamp of Merauke and surrounding area had a good nutritional value.
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.663 KB) | DOI: 10.15578/iaj.4.1.2009.19-24


Pearl oyster, Pinctada maxima is one of economical ly important species in aquaculture, particularly in pearl industry. Information on genetic variation of pearl oyster is required in order to be able to make a sound management of it’s natural populations and to utilize it to improve the quality of pearl culture. Five populations from different geographic locations of pearl oyster, Pinctada maxima, (Sumbawa, Bali, Selat Sunda, Belitung, and South Sulawesi) were analyzed for genetic variation within a 750-base pair region of the Mitochondrial Cytochrome Oxidase subunit I (MtCOI) gene using Restriction Fragment Length Polymorphism (RFLP) technique. The analysis of 25 pearl oyster samples, their haplotype diversity ranged from 0.0970 to 0.1939 and the number of haplotype in each population ranged from three to five haplotypes. Clustering of populations based on Nei’s genetic distances and constructed using unweighted pair-group method with Arithmetic mean (UPGMA) showed that the populations were clustered into two groups: Belitung, Selat Sunda, Bali and Sumbawa in one group, while South Sulawesi in the second group.
RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii Imron, Imron; Sunandar, Dadan; Susilowati, Rini; Suprapto, Rommy; Khasani, Ikhsan
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.592 KB) | DOI: 10.15578/iaj.4.2.2009.93-100


Indonesia is rich of giant fresh water prawn (GFP) germ plasms. Best utilization of these resources for the purpose of either aquaculture development or conservation of genetic resources requires some information on the structure and levels of their genetic diversity. This study was aimed to characterize those GFP genetic resources by applying RAPD genetic markers. Six Indonesian populations of GFP from Asahan, Barito, Ciasem, Ogan, GImacro and Papua were collected and analyzed for their genetic variation using five RAPD primers. The results showed the diversity within the populations, as revealed by the level of polymorphism, ranged from 29% to 76% while genetic divergence between populations as shown by genetic distance ranged from 0.04 to 0.50. In terms of genetic divergence, two genetically distinct groups of GFP, namely the Papua GFP in one group and the remaining five GFP populations in the other, were identified. The results also showed the presence of specific population markers that are useful for genetic identification of GFP populations. Implication of these finding with regard to breed development is discussed.
KARAKTERISTIK GENETIK POPULASI TIRAM MUTIARA (Pinctada margaritifera) TERKAIT DENGAN DISTRIBUSI GEOGRAFISNYA DI PERAIRAN INDONESIA Susilowati, Rini; Sumantadinata, Komar; Soelistyowati, Dinar Tri; Sudradjat, Achmad
Jurnal Riset Akuakultur Vol 4, No 1 (2009): (April 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.112 KB) | DOI: 10.15578/jra.4.1.2009.47-54


Tujuan penelitian ini untuk memetakan keragaman genetik lima populasi tiram mutiara di Indonesia (Sumbawa, Bali Utara, Selat Sunda, Belitung, Sulawesi Selatan) dengan teknik mtDNA RFLP daerah amplifikasi Cytochrome Oxydase I (COI) dan hubungan kekerabatannya. Lima puluh tiram mutiara (Pinctada margaritifera) yang dianalisis menghasilkan DNA teramplifikasi sebesar 750 pb pada daerah COI mtDNA dengan teknik RFLP. Delapan belas komposit haplotipe terdeteksi dengan menggunakan tiga enzim restriksi: FokI, HaeIII, dan NlaIV. Diversitas haplotip rata-rata sebesar 0,255±0,093. Lima populasi tiram mutiara menghasilkan tiga kelompok dengan jarak genetik terendah adalah populasi Sumbawa dan Bali Utara (0,017) dan terjauh adalah populasi Sulawesi Selatan (0,142). Populasi Sulawesi Selatan merupakan populasi unik berdasarkan distribusi haplotipe BBCAA (60%) dengan nilai keragaman genetik terendah (0,105) dibandingkan dengan populasi lainnya (0,177-0,328).The objectives of this study were to map the genetic diversity of five populations of pearl oyster in Indonesian waters using restriction fragment length polymorphism analysis of DNA COI gene and their genetic relationships. A total of 50 individual of pearl oysters (Pinctada margaritifera) were analyzed for genetic variations within a 750-base pair region of the mitochondrial DNA COI gene using restriction fragment length polymorphism analysis. 18 composite haplotypes were detected following three digestions of endonuclease: FokI, HaeIII, and NlaIV. Five populations of pearl oysters formed three groups where the lowest values of Nei’s genetic distance were among Sumbawa and North Bali populations (0.017) and highest were among the South Sulawesi populations (0.142). The South Sulawesi populations possess uniqueness based on the haplotipe distribution of BBCAA (60%) with the lowest values of genetic diversities (0.105) compared to other populations (0.177--0.328).
Jurnal Imiah Pendidikan dan Pembelajaran Vol 2, No 1 (2018): April 2018
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.601 KB) | DOI: 10.23887/jipp.v2i1.13870


This type of research  is a class action research. The purpose of this research enhances students critical thinking ability by application of Problem Based Learning with  Audio Visual Media. Data were collected by using a sheet of observation and evaluation tests. The average percentage of critical thinking ability of students overall observation sheet on precyae 13.8% increase in cycle cycle I 69% and increased again in cycle II 96.5%. And the overall percentage on the test results the evaluation cycle I 44.8% increase in cycle II 96.6%. Thus the results showed that the application of problem based learning with audio visual media is able to enhance the critical thinking ability of students.