Pinandi Sri Pudyani, Pinandi Sri
Bagian Ortodonsia Fakultas Kedokteran Gigi Universitas Gadjah Mada

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Journal : Journal of Dentistry Indonesia

PENGARUH KEKURANGAN PROTEIN TERHADAP REMODELING TULANG ALVEOLUS (Eksperimental Aktivitas Osteoblas Dan Selosteoklas) Pudyani, Pinandi Sri
Journal of Dentistry Indonesia Vol 10, No 2 (2003): AUGUST
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.058 KB) | DOI: 10.14693/jdi.v10i2.485

Abstract

The aim of the study was to investigate the influence of protein deficiency on osteoblast and osteoclast cells activity on Sprague Dawley rats model alveolar bone. The study was carried out on 10 Spraque Dawley rats, divided in 2 groups. The first group was fed by died 4% protein after weaning period until animals aged 56 days. The other group was fed by standard diet. Osteoblas and osteoclast cells activity were counted by histological analysis. The result of the study from one-way Anova showed the significant differences (p<0,05) osteoblasts and osteoclasts activity between the protein deficiency group and the standard group.
Pengaruh Kekurangan Kalsium terhadap Daya Reversibilitas Kalsifikasi Tulang sebagai Faktor Penunjang Keberhasilan Perawatan Ortodontik Pudyani, Pinandi Sri
Journal of Dentistry Indonesia Vol 12, No 1 (2005): April
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3260.944 KB) | DOI: 10.14693/jdi.v12i1.858

Abstract

The growth and development of bone play an important role in orthodontics, where bone calcification can be used the estimate bone maturity for diagnosis and treatment planning. Nutritional status during pregnancy and infant period will influence the growth and development of bone. Calcium diet is an important factor determining optimal calcification during bone growth and development stages. The purpose of this study was to investigate the possibility to restore calcium and phosphorus in bone after pre- and postnatal calcium deficiency. Three groups of Rattusnorvegicus rats were used in the experiments. The first group was the control group with standard diet, the second was infant group with pre- and postnatal calcium deficiency, and the third group consisted of young rats at weaning age with pre- and postnatal calcium deficiency but supplemented with enough calcium in the diet. Bone calcification stages were analyzed 1) histologically by measuring epiphyseal width on right femur, 2) by measuring calcium and phosphorus concentration on left femur with spectrophotomethry, atomic absorbtion and UV spectroscopy. The data were analyzed by one way Anova and t testing. The results showed that there were significant differences in the epiphyseal width and in calcium and phophorus concentrations between all three groups (p<0.01). It was concluded that bone calcification damage because of pre- and postnatal calcium deficiency was an irreversible process. Calcium supplement after bone calcification could not restore the condition.
Pengaruh Kekurangan Protein Pre dan Postnatal Terhadap Mineralisasi Gigi Pudyani, Pinandi Sri
Journal of Dentistry Indonesia Vol 8, No 2 (2001): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3410.314 KB) | DOI: 10.14693/jdi.v8i2.920

Abstract

The accuracy of nutrition quantity during and after pregnancy is needed for supporting division, differentiation and replication of cells during growth stage. Protein is needed to obtain optimally childs body growth and development including tooth. The study was aimed to deteremine the effects of pre and postnatal protein deficiency on tooth mineralization rats model. The study was carried out on 30 Rates norvegicus rats, divided in 3 groups.The first group was fed the protein deficient diet (4%) during pre and postnatal period, the second was fed the protein deficient diet (4%) only postnatal and the third was fed the postnatal diet. Feeding was carried out until animales aged at 56 days. After that, animals were sacrificed and the width of right mandibular molar prevention layer was histologically analyzed to know the number of tooth mineralization. The result of the study showed significant differences (p<0.05) in width of prevention layer between standard and experimental groups. Its concluded that pre and postnatal protein deficiency were inhibits tooth mineralization.
Pengaruh Kekurangan Kalsium Pre-Postnatal terhadap Kepadatan Gigi dalam Menunjang Perawatan Ortodontik Studi Eksperimental Laboratoris pada Tikus Pudyani, Pinandi Sri
Journal of Dentistry Indonesia Vol 8, No 1 (2001): April
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3697.029 KB) | DOI: 10.14693/jdi.v8i1.938

Abstract

The knowledge of the growth and development factors in orthodontics is needed because skeletal manipulations are often done in orthodontic treatment. Because of that, knowledge and understanding of the concept of physiological growth by age especially in the face is needed, apart from growth and development of the teeth. The purpose of this study was to investigate the influence of pre and postnatal calcium deficiency on the density of teeth. Density of teeth was measured by calcium and phosphorus concentration and right incisivus maxilla weight. The study was carried on 30 Rattus norvegicus rats, divided in 3 groups. The first group was fed by a diet without calcium postnatal, the second group was fed by a diet without pre and postnatal calcium, and the third group was fed by standard diet. The concentration of calcium was measured by AAS (Atomic absorption spectrophotometric analysis), the right incisivus maxilla was measured in milligrams, and phosphorus concentration by ultraviolet spectrophotometry. The results of the study showed a significant difference (p<0,01), in calcium and phosphorus concentration and right incisivus maxilla weight between the non calcium fed postnatal, pre-postnatal and the standard groups.