Fathin Hamida, Fathin
Bogor Agricultural University

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SELECTION OF LACTIC ACID BACTERIA AS PROBIOTIC CANDIDATE FOR CHICKEN Hamida, Fathin; Wiryawan, Komang G; Meryandini, Anja
Media Peternakan Vol. 38 No. 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.879 KB) | DOI: 10.5398/medpet.2015.38.2.138

Abstract

Lactic acid bacteria (LAB) regarded as safe microorganisms; they can naturally live in gastrointestinal tract, so appropriately used as a probiotic for chicken. This study aimed to select six isolates of LAB (E1223, E3, E4, E5, E7, and E8) to obtain the isolates potentially as probiotic candidate for chicken. The six isolates were derived from spontaneous fermented corn obtained from Laboratory of Animal Biotechnology and Biomedical, PPSHB, Bogor Agricultural University, Indonesia. LAB isolates were tested their susceptibility to antibiotics (bambermycin, erythromycin, chloramphenicol, and tetracycline) then were examined in vitro for their tolerance to gastrointestinal pH (2, 3, 4, and 7.2) and 0.5% bile salt condition, antimicrobial activity against Salmonella enteritidis and Enterococcus casseliflavus, and ability to adhere to chicken ileal cells. The results showed the isolates E5, E7, and E8 were sensitive to tetracycline and chloramphenicol, they could survive at pH 2, 3, 4, and 7.2, could survive at 0.5% bile salts, produced antimicrobial activity, and able to adhere to ileal cells (9.40±0.00 Log CFU/cm2 of E8) and were significantly (P<0.05) higher than those of control (5.30±0.14 Log CFU/cm2). In conclusion, this study showed that isolate E8 had better potential compared to isolates E5 and E7 in most in vitro assays as a probiotic candidate for chicken. E5, E7, and E8 were closely related with Pediococcus pentosaceus based on 16S rRNA gene.Key words: LAB, probiotic, chicken, in vitro
ESCHERICHIA COLI RESISTEN ANTIBIOTIK ASAL AIR KERAN DI KAMPUS ISTN Hamida, Fathin; Aliya, Lisana Sidqi; Syafriana, Vilya; Pratiwi, Della
Jurnal Kesehatan Vol 12, No 1 (2019): Jurnal Kesehatan
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/jk.v12i1.8958

Abstract

Resistensi antibiotik menjadi masalah global yang menyebar luas di dunia. Lingkungan merupakan jalur transmisi dan menyebabkan resistensi antibiotik. Hal itu dapat disebabkan oleh buruknya infrastruktur limbah air dan kontaminasi fekal. Kontaminasi air berpengaruh buruk bagi kesehatan manusia. Kampus ISTN memiliki pipa saluran air keran yang jarak berdekatan dengan pipa? septic tank, jarak antara pipa air keran dengan pipa septic tank berkisar ? 11 meter. Kontaminasi air oleh Escherichia coli menjadi factor penurunan kualitas air. Penelitian ini bertujuan mengisolasi E. coli dari pipa air keran kampus ISTN dan mendeteksi resistensinya terhadap antibiotik. Empat isolate E. coli telah diisolasi menggunakan media Lactose Broth dan Chromogenic Coliform Agar. Empat isolat E. coli telah diidentifikasi secara biokimiawi. Empat isolate E. coli diuji sensitivitasnya terhadap antibiotik amoksisilin, tetrasiklin, kloramfenikol, dan siprofloksasin menggunakan metode difusi cakram. Sensitivitas antibiotik diinterpresikan berdasarkan standar CLSI (Clinical and Laboratory Standard Institute). Hasil menunjukkan bahwa isolat 1A, 2A, 3A, dan 4A resisten terhadap amoksisilin, dan isolat 1A bersifat intermediat.