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APLIKASI AMONIUM HIDROKSIDA (NH4OH) SEBAGAI TRIGGER PEMIJAHAN TIRAM MUTIARA Pinctada maxima (JAMESON) Winanto, Tjahjo

Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.428 KB)

Abstract

Penelitian ini dilakukan dengan tujuan untuk mengetahui dosis larutan NH4OH yang optimum dan posisi injeksi yang tepat sehingga dapat menjadi pemicu (trigger) pemijahan induk tiram mutiara Pinctada maxima (Jameson). Percobaan menggunakan Rancangan Acak Lengkap Faktorial (Faktorial-RAL), dengan tiga kali ulangan. Perlakuan yang diterapkan terdiri dari dua faktor yaitu (I) Posisi Injeksi dan (II) Dosis larutan NH4OH. Faktor I terdiri dari 4 taraf faktor yaitu posisi injeksi di bagian (A) Otot aduktor, (B) Pangkal kaki, (C) Gonad dan (D) Tidak diinjeksi (kontrol). Faktor II terdiri dari 3 taraf faktor yaitu dosis (E) 0,5 ml, (F) 1,0 ml dan (G) 1,5 ml. Hasil pengamatan menunjukkan bahwa dosis larutan NH4OH yang optimum untuk memicu (trigger) pemijahan induk tiram mutiara Pinctada maxima (Jameson) adalah 0,5-1,0 ml dengan posisi injeksi terbaik di bagian pangkal kaki.Kata kunci : Pearl oyster Pinctada maxima; amonium hidroksida (NH4OH); spawning
RESPON LARVA TIRAM MUTIARA PINCTADA MAXIMA (JAMESON) TERHADAP BERBAGAI TINGKAF INTENSITAS CAHAYA Winanto, Tjahjo; Soedharma, Dedy; Affandi, Ridwan; Sanusi, Harpasis S.
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 4 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17.45 KB) | DOI: 10.14710/ik.ijms.13.4.197-202

Abstract

Intensitas cahaya berpengaruh terhadap fungsi fisiologis dan struktur larva bivalvla. Penelitiaan ini dilakukan untuk mengetahui pengaruh intensitas cahaya terhadap distribusi, pertumbuhan dan sintasan larva tiram mutiara Pinctada maxima. Rancangan acak lengkap dengan empatperlakuan intensitas cahayayaitu 0 (ditutup rapat), 200, 500, dan 800 lux, masing-masing dengan tiga ulangan diterapkan pada penelitian ini. Hasil penelitian menunjukkan bahwa distribusi larva dipengaruhi oleh intensitas cahaya, sedangkan intensitas cahaya optimum untuk sintasan dan pertumbuhan larva adalah 0-200 lux.Kata kunci : Larva, Pinctada maxima, respon, intensitas cahaya, distribusi, sintasan, pertumbuhan
MICROENCAPSULATION OF SINGLE-CELL PROTEIN FROM VARIOUS MICROALGAE SPECIES Sukardi, Purnama; Winanto, Tjahjo; Hartoyo, ,; Pramono, Taufik Budhi; Wibowo, Eko Setyo
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3254.576 KB) | DOI: 10.19027/jai.13.115-119

Abstract

ABSTRACT The objective of the research was to evaluate nutritional values of microencapsulated diet made from single cell protein of microalgae. Complete randomized design was applied using three different types of microalgae for inclusion trials i.e. (A) Nannochloropsis sp., (B) Chlorella sp., and (C) Spirulina sp. with five replications respectively. Microencapsulated diet was produced by a modification method based on thermal cross-linking with stable temperature. Phytoplankton was cultured in sea water for which fertilized by a modification of Walne and Guillard fertilizer. The results showed that the highest value of nutrition content was Spirulina sp. and the average composition of protein, crude lipid, carbohydrate, ash, nitrogen free extract, and water content was 34.80%, 0.30%, 18.53%, 20.09%, 26.29%, and 13.32%, respectively. Organoleptically, microcapsule showed that the color of capsule was dark green and smell fresh phytoplankton. Keywords: microcapsule, single-cell protein, thermal cross-linking, microalgae, phytoplankton  ABSTRAK Tujuan penelitian adalah mengevaluasi kandungan nutrisi pakan mikrokapsul protein sel tunggal (single cell protein) yang berasal dari berbagai jenis mikroalga (fitoplankton). Rancangan percobaan yang digunakan adalah rancangan acak lengkap, dengan perlakuan inklusi mikrokapsul dari jenis fitoplankton (A) Nannochloropsis sp., (B) Chlorella sp., dan (C) Spirulina sp., masing-masing diulang lima kali. Pembuatan mikrokapsul dilakukan dengan menggunakan modifikasi metode dasar thermal cross-linking, serta menerapkan teknik pengeringan suhu konstan. Proses pembuatan mikrokapsul protein diawali dengan kultur fitoplankton jenis Nannochloropsis sp., Chlorella sp., dan Spirulina sp. Kultur dilakukan di dalam laboratorium menggunakan media air laut dan modifikasi pupuk Walne dan Guillard. Hasil penelitian menunjukkan bahwa kandungan nutrisi tertinggi terdapat pada jenis mikrokapsul protein sel tunggal yang berasal dari Spirulina sp., dengan rata-rata komposisi kandungan protein 34,80%, lemak 0,30%, karbohidrat 18,53%, abu 20,09%, dan BETN 26,29%. Mikrokapsul berwarna hijau tua dan aroma fitoplankton segar. Kata kunci: mikrokapsul, protein sel tunggal, thermal cross-linking, mikroalga, fitoplankton
RASIO PERTUMBUHAN RUMPUT LAUT Gracilaria sp PADA SKALA LABORATORIUM DAN LAPANG DI TAMBAK SILVOFISHERY Dewi, Rose; Winanto, Tjahjo
Jurnal Harpodon Borneo Vol 6, No 1 (2013): Volume 6 No 1 April 2013
Publisher : Jurnal Harpodon Borneo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.666 KB) | DOI: 10.35334/harpodon.v6i1.11

Abstract

Penurunan kualitas perairan pada tambak Silvofishery disebabkan kepadatan biota budidaya yang terlalu tinggi dan adanya kelebiha pemberian pakan. Upaya penanggulangan permasalahan untuk meminimalisir kondisi tersebut dilakukan usaha  teknologi biofilter menggunakan rumput Gracilaria sp (Rhodophyta). Untuk dapat mengoptimalkan teknis monitoring pada setiap tahapan budidaya, maka dibandingkan dengan skala laboratoris. Perlakuan penelitian skala laboratorium (A), sedangkan perlakuan di skala lapang (Perlakuan B) dilakukan di tambak Silvofishery, dengan ketentuan umur serta ukuran biota budidaya yang sama. Gracilaria sp yang ditanam dengan metode lepas dasar (bottom method). Monitoring dilakukan selama masa pemeliharaan 60 hari,  dilakukan pengamatan terhadap pertumbuhan rumput laut (biomassa basah).  Analisis data pertumbuhan menggunakan uji T-student. Hasil pertumbuhan Gracilaria sp, rata-rata per 15 hari menunjukkan bahwa pertambahan berat berbeda nyata antar perlakuan (P £ 0,05), yang dipengaruhi oleh respon proses fotosintesis dan penyerapan kandungan Nitrogen (ammonia, nitrat dan nitrit). Pertumbuhan Gracilaria sp lebih tinggi dalam skala laboratoris mencapai 28 gram dibandingkan di lapang pada area tambak silvofishery 24,78 gram.
DOMESTICATION OF RED SEAWEED (GELIDIUM LATIFOLIUM) IN DIFFERENT CULTURE MEDIA Wijayanto, Andri; Widowati, Ita; Winanto, Tjahjo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 25, No 1 (2020): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.25.1.39-44

Abstract

Gelidium latifolium is one of red seaweed types potentially can be developed as an industrial raw material. Since Gelidium is currently taken from ocean, the availability of seaweed from aquaculture is necessary to overcome the small number of its availability in nature. In Indonesia, G. latifolium cultivation has not been carried out so that domestication is required. The use of macro and micro nutrients in growth media is essentially needed for the domestication process. Domestication requires fast media and place for growth. The purpose of this study is to determine the growth of biomass and the survival of G. latifolium in different culture media. The study was conducted in a semi-outdoor research laboratory. The method used in this research is laboratory experimental method and Completely Randomized Design (CRD) with the treatment applied using 3 types of culture media (Urea: Za: TSP) by comparison (A) 100: 50: 50% (2 g.L-1), (B) 75: 75: 50% (2 g.L-1) and (C) 75: 50: 75% (2 g.L-1), with 3 replications. The seaweed was kept in 10 L of water in aeration equipped aquarium and filled with 10 g of G. latifolium on each treatment. The best growth rate of G. latifolium biomass is 5.67± 0.58 g and 100±0% are survived in C culture medium with a concentration of 75% Urea: 50% ZA: 75% TSP (2 g.L-1).
Kepadatan Optimum dan Morfologi Spat Tiram Mutiara Pinctada maxima (Jameson) pada Pemeliharaan dengan Tingkat Kepadatan Berbeda Winanto, Tjahjo; Marasabessy, Mohammad Djen; Dodi, Safar
Journal Omni-Akuatika Vol 12, No 3 (2016): OmniAkuatika special edition Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.529 KB) | DOI: 10.20884/1.oa.2016.12.3.198

Abstract

Stocking density was affected to the growth and survival rate of spat. The objective of this study is to obtain information optimal stocking density and morphology of spat during nursery culture. Randomized block design was applied with four stocking density treatments i.e. (A) 500, (B) 1000, (C) 1500, (D) 2000 individu (ind) collector-1,  conducted in triplcate The result showed that optimum density of P. maxima spat was 500 ind collector-1. The best of survival rate was found at density 500 ind collector-1 and the best of growth at treatment 500 ind collector-1 (36.50 x 33.37 mm). At the density of 500 ind collector1  shell morphology was normal, otherwise at high density (1,500 – 2,000 ind collector1) slower growth spat, shells shape is unnormal, for example  the shape of an elongated shell (DV> AP), or widened shape (DV <AP).
Analisis Perubahan Lahan Kawasan Laguna Segara Anakan Selama Periode Waktu (1978 – 2016) Menggunakan Satelit Landsat Multitemporal Dewi, Rose; Zainuri, Muhammad; Anggoro, Sutrisno; Winanto, Tjahjo
Journal Omni-Akuatika Vol 12, No 3 (2016): OmniAkuatika special edition Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.104 KB) | DOI: 10.20884/1.oa.2016.12.3.209

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Segara Anakan Lagoon (SAL) has the unique characteristics of the region with the high of natural resources biodiversity. The region of SAL is influences by natural factor (estuarine freshwater stream of Citanduy watershed and the high of sedimentation rate) and the variety of anthropogenic factor. The factors lead to the changes in land, because it triggers the decrease of the lagoon water bodies. The aim of the research is to know the changes of the region land use in SAL and its impact. Temporally spatial approach is required to evauate the influence of both factors. Spatial approach is done by using remote sensing method with Geographic Information System, with Multi-Temporal Landsat imagery data processing (the year of 1978, 1994, 2001, 2009, 2011 and 2016). It uses land classification technique (Supervised Classification). It is processed with ER Mapper 7.0 software and displayed with Arc GIS (Jpeg). The analysis result shows that there is 2.703,7 ha extensive shrinkage lagoon in 38 year (1978 - 2016) or there is 71.15 ha per year in land area increase in the last 38 years. It is supported with 4.741,36 ha residential land and 17.962,64 ha field land that cause the degredation of SAL region. The increase of SAL citizenary amount triggers the land conversion for the interest of the various anthropogenic activities. It is supported by the threat of natural factors of high sedimentation level that cause higher SAL pressure. In conclusion, it is needed evaluation and management strategies to overcome the changes of SAL land area by the local government and related parties.
Pengaruh Suhu dan Salinitas Terhadap Respon Fisiologi Larva Tiram Mutiara Pinctada maxima (Jameson) Winanto, Tjahjo; Soedharma, Dedi; Affandi, Ridwan; Sanusi, Harpasis S.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.755 KB) | DOI: 10.14203/jbi.v6i1.3171

Abstract

ABSTRACTPENDAHULUANPinctada maxima adalah spesiesakuakultur yang mempunyai nilaiekonomi tinggi (Taylor et al. 1997). Dipasaran internasional, mutiara yangdiproduksi sering kali disebut dengannama “South Sea Pearl”. Indonesiatermasuk salah satu negara penghasilmutiara (South Sea Pearl) yang cukupdiskenal di pasaran dunia, sebagian besarproduksi South Sea Pearl yang dipasarkanberasal dari hasil budidaya (Anna2006). Produksi mutiara berbasisbudidaya merupakan aktivitas usaha yangmenguntungkan. Perkembangan usahabudidaya mutiara saat ini sudah mengarahpada kegiatan industri yang terintegrasi(Fassler 1995).The Effect of Temperature and Salinity to The Physiological Respons on The Larvae ofPinctada maxima (Jameson). Energy budget is one of the most sensitive tools available forindividual assessing environmental changes like temperature and salinity, and also prerequisitefor individual growth and survival. The aim of this study is to obtained information on energybudget on routine metabolism, in different levels of temperature and salinity, and to know thelevels of optimum temperature and salinity. The research was used randomized block design,with three replications. The result showed that optimal temperature and salinity on P. maximalarvae was 28 oC and 32 – 34 ‰ (BE and BF). Energy budget to routine metabolism increasedwas attributed to increased temperature and salinity due to the optimal, than would be decreasedwhen temperature and salinity increased. The highest of energy budged for routine metabolismat treatment BF. Stage I: energy budged between 6.73 – 7.35 C g wet weight-1 hour-1 (28.18 –30.74 J g wet weight-1 hour-1); Stages II: 5.85 – 5.95 C g wet weight-1 hour-1 (24.48 – 24.90 J g wetweight-1 hour-1); Stages III: 4.73 – 4.80 C g wet weight-1 hour-1 (15.07– 19.58 J g wet weight-1hour-1). The highest survival rate of larvae was by treatment BF, but has not higher significant(P e” 0.05) with BE, stage I: survival rate between 87.75 – 87.92 %; Stage II: 81.91 – 82.39 % andstage III: 76.72 – 77.26 %. The best of relative growth length of larvae by treatment BF and notsignificant (P e” 0.05) with BE, at stage I: 29.78 x 17.93 ?m – 30.57 x 18.43 ?m (AP x DV); stageII: 57.62 x 46.73 ?m – 58.13 x 47.33 ?m and stage III: 80.32 x 69.29 ?m – 80.88 x 69.62 ?m. Thequickest time of plantigrade stages have found by treatment BF (day 19.50) and hasn’tsignificant (P > 0.05) with BE (day 20.85).Keywords: Pinctada maxima, larvae, response; physiology, metabolism.Kata kunci: Pinctada maxima, larvae, respon, fisiology, metabolisme.
Pengaruh Kedalaman Terhadap Proses Pelapisan Inti Bulat Pada Kerang Air Tawar (Anodonta woodiana) Rachman, Boedi; Winanto, Tjahjo; Maskur, Maskur; Sukmajaya, Yade
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.952 KB) | DOI: 10.14203/jbi.v6i1.3172

Abstract

ABSTRACTThe Effect of Depth to Deposition Process on Round Nucleus of Fresh Water Mussel (Anodontawoodiana). One of the affecting factors to the quality of pearl culture is the thickness of pearldepositions (nacre). The objective of this study was to obtain information on best level ofdepth to culture of pearl, to get fast nacre deposition and high quality of pearl. The researchwas conducted for 9 months, in the freshwater pond, was 300 m2 wide and 1 m deep. Freshwaterpearl Anodonta woodiana, sized ranging from 12 – 15 cm were studied. Completely randomizeddesign was used with levels of deep treatment (A) 30 cm; (B) 60 cm and (C) 90 cm. The resultshowed that best thickness of pearl deposition by 90 cm deep (1.30 mm) but hasn’t biggestsignificant (P>0.05) to the deep of 60 cm (1.10 mm) and biggest significant (P< 0.05) to the deepof 30 cm (0.70 mm). The result of implantation was followed that 30, 60 and 90 cm deep were 11.9%; 12.2 %; 12.0 %, whereas survival rate was followed 79.2 %; 79 % and 78.7 %.Keywords: Freshwater mussel; Anodonta woodiana; effect; level of deepKata kunci: Kerang air tawar, Anodonta, woodiana, kedalaman laut
RASIO PERTUMBUHAN RUMPUT LAUT GRACILARIA SP PADA SKALA LABORATORIUM DAN LAPANG DI TAMBAK SILVOFISHERY Dewi, Rose; Winanto, Tjahjo
Jurnal Harpodon Borneo Vol 6, No 1 (2013): Volume 6 No 1 April 2013
Publisher : Jurnal Harpodon Borneo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v6i1.11

Abstract

Penurunan kualitas perairan pada tambak Silvofishery disebabkan kepadatan biota budidaya yang terlalu tinggi dan adanya kelebiha pemberian pakan. Upaya penanggulangan permasalahan untuk meminimalisir kondisi tersebut dilakukan usaha  teknologi biofilter menggunakan rumput Gracilaria sp (Rhodophyta). Untuk dapat mengoptimalkan teknis monitoring pada setiap tahapan budidaya, maka dibandingkan dengan skala laboratoris. Perlakuan penelitian skala laboratorium (A), sedangkan perlakuan di skala lapang (Perlakuan B) dilakukan di tambak Silvofishery, dengan ketentuan umur serta ukuran biota budidaya yang sama. Gracilaria sp yang ditanam dengan metode lepas dasar (bottom method). Monitoring dilakukan selama masa pemeliharaan 60 hari,  dilakukan pengamatan terhadap pertumbuhan rumput laut (biomassa basah).  Analisis data pertumbuhan menggunakan uji T-student. Hasil pertumbuhan Gracilaria sp, rata-rata per 15 hari menunjukkan bahwa pertambahan berat berbeda nyata antar perlakuan (P £ 0,05), yang dipengaruhi oleh respon proses fotosintesis dan penyerapan kandungan Nitrogen (ammonia, nitrat dan nitrit). Pertumbuhan Gracilaria sp lebih tinggi dalam skala laboratoris mencapai 28 gram dibandingkan di lapang pada area tambak silvofishery 24,78 gram.