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POTENSI EKSTRAK ETANOL BAWANG MERAH (ALLIUM ASCOLONICUM L.) DAN GARAM NACL MENURUNKAN LUAS AREA SERTA MENINGKATKAN KONTRAKSI JARINGAN LUKA BAKAR RINGAN Sukadana, I M.; Rahayu, S.; Melli, Melli
Jurnal Kimia (Journal of Chemistry) Vol.13 No.1 Januari 2019
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2019.v13.i01.p09

Abstract

Luka bakar (burns) merupakan respon kulit dan jaringan subkutan terhadap trauma suhu atau termal yang berdampak pada kerusakan kulit. Berat atau ringannya kerusakan jaringan akibat luka bakar dipengaruhi oleh beberapa faktor antara lain kedalaman luka bakar, luas luka bakar, lokasi luka bakar, kesehatan tubuh secara umum, mekanisme cedera dan usia. Luas area luka bakar dan persentase kontraksi luka merupakan indikator proses penyembuhan luka bakar ringan. Penelitian ini menggunakan 25 ekor tikus wistar yang dibagi menjadi 5 kelompok perlakuan dengan rancangan randomized posttest only control group design sebagai berikut; K: diberi perawatan vaselin (kontrol negatif), Kp: diberi perawatan bioplacenton (kontrol positif), P1: diberi perawatan garam dapur pada konsentrasi 7,5% dalam vaselin, P2: diberi perawatan ekstrak bawang merah pada konsentrasi 40% dalam vaselin, dan P3: diberi perawatan campuran ekstrak bawang merah 40% dan garam dapur pada konsentrasi 7,5% dalam vaselin. Hasil penelitian dan analisis statistik One-Way ANOVA dan Post Hoc Test LSD menggunakan program SPSS for Windows versi 19 menunjukkan kelompok perlakuan P2 yaitu ekstrak etanol bawang merah dengan konsentrasi 40% memberikan hasil terbaik sehingga sangat berpotensi untuk dikembangkan lebih lanjut sebagai bahan obat luka bakar ringan dibandingkan kelompok perlakuan P1 maupun P3 karena kemampuannya menurunkan luas area dari 340,79 mm2 dihari ke-1 menjadi 11,75 mm2 serta meningkatkan kontraksi luka sebesar 81,59% di akhir perawatan.
SINGLE NUCLEOTIDE POLYMORPHISMS OF LIPOPROTEIN LIPASE GENE AND ITS ASSOCIATION WITH MARBLING QUALITY IN LOCAL SHEEPS Hidayati, H.; Sumantri, C.; Noor, R. R.; Priyanto, R.; Rahayu, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.929 KB) | DOI: 10.14710/jitaa.40.1.1-10

Abstract

Lipoprotein lipase (LPL) is a key enzyme that plays in metabolism and transport lipoprotein andtherefore has an influence on blood triglyceride levels. LPL controls triacylglycerol partitioning betweenadipose tissue and muscle that increases fat storage or provides energy in the form of fatty acids formuscle growth. The research was aimed to explore Single Nucleotide Polymorphisms of LPL gene andto associate SNP with marbling quality. A total of 66 genomic DNAs consisted of sumatera thin-tail edsheep (50 heads) and garut sheep (16 heads) were used in this study. Polymerase Chain Reaction wasused to amplify genomic DNA and direct sequencing method was to identify polymorphism sequences.The sequences were analyzed with Bio Edit and MEGA 5.2. The BLAST sequence was obtained fromgene bank X.68308.1. The association between the genotype and marbling quality was analyze by oneway ANOVA and further between mean differences were tested using least sgnificant difference. Theresults showed that 3 novel SNPs i.e. insertion g.26>C; insertion g.27> G and c.192T>C on garut sheepand a SNP insertion g.26>C/G on sumatera thin-tail ed sheep. The diversity of LPL gene at c.192T>Cwas associated with heneicosanoic acid, whereas TT genotype (0.04%) was higher than CC (0.03%) andCT (0.02%).
Kesejahteraan Domba Akibat Pencukuran: Tingkah laku Domba Sebelum, Sesaat dan Setelah Pencukuran Bulu Yamin, M.; Rahayu, S.; Ma'ani, A.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol 1, No 1 (2013): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.1.1.15-18

Abstract

THE EFFECT OF 5E LEARNING CYCLE INSTRUCTIONAL MODEL USING SOCIOSCIENTIFIC ISSUES (SSI) LEARNING CONTEXT ON STUDENTS’ CRITICAL THINKING Cahyarini, A.; Rahayu, S.; Yahmin, Y.
Jurnal Pendidikan IPA Indonesia Vol 5, No 2 (2016): October 2016
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v5i2.7683

Abstract

The aim of this study was to investigate the effect of 5E learning cycle instructional model using socioscientific issues (SSI) learning context on students’ critical thinking skills of acid-base. This study used quasi-experimental posttest only control group design. The sample consisted of three classes, which were XI MIA-4class (n = 32) that learned using 5E LC model, XI MIA-5 class (n = 33) that learned using 5E LC+SSI, and XI MIA-6 class (n = 32) that learned using conventional method. The samples were choosen by convenience sampling technique. The test instrument consisted of 15 multiple choice items which were valid and reliable (r = 0.806). The data were analyzed using one way ANOVA test and LSD posthoc test. The results of this study indicated that the students who learned using 5E LC+SSI model showed greater levels of critical thinking skills (  = 74,95) than both the student who learned using 5E LC model (  = 74,17) and  the student who learned using conventional method (  = 68,96). Based on statistics analysis, there was significant differences on students’ critical thinkings between students taught using conventional method and students taught either using 5E LC+SSI model and 5E LC model. However,  there was no significant differences on students’ critical thinking skills between students taught using 5E LC+SSI model and the students taught using 5E LC model.
SOCIOSCIENTIFIC ISSUES (SSI) IN REACTION RATES TOPIC AND ITS EFFECT ON THE CRITICAL THINKING SKILLS OF HIGH SCHOOL STUDENTS Pratiwi, Y. N.; Rahayu, S.; Fajaroh, F.
Jurnal Pendidikan IPA Indonesia Vol 5, No 2 (2016): October 2016
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v5i2.7676

Abstract

The aim of this research was to investigate the difference in students’ critical thinking which learnt cooperatively using different learning context. Quasy experimental postest only control group design was chosen as experimental design using two equivalent classes as the sample. The first class (15 MIA 5, n = 30) was taught using socioscientific issues (SSI) as the learning context and the second one (15 MIA 6, n = 30) was not. The research instrument was a test consisted of 16 items of multiple choice questionsdeveloped based on Ennis’ critical thinking indicators. This instrument have been validated and its reliability approved with r = 0,765. Students’ post-test scores were analyzed using t-test with SPSS 16 for Windows. The result showed that statistically students’ critical thinking skills betweentwo classes was significanly different (Asym. Sig = 0,037). Students who taught using SSI have higher score (73,96) than the one wasn’t (66,04). Result shows that using socioscientific issues (SSI) as learning context has significant effect on high school students’ critical thinking skills.
THE EFFECT OF 5E LEARNING CYCLE INSTRUCTIONAL MODEL USING SOCIOSCIENTIFIC ISSUES (SSI) LEARNING CONTEXT ON STUDENTS CRITICAL THINKING Cahyarini, A.; Rahayu, S.; Yahmin, Y.
Jurnal Pendidikan IPA Indonesia Vol 5, No 2 (2016): October 2016
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v5i2.7683

Abstract

The aim of this study was to investigate the effect of 5E learning cycle instructional model using socioscientific issues (SSI) learning context on students critical thinking skills of acid-base. This study used quasi-experimental posttest only control group design. The sample consisted of three classes, which were XI MIA-4class (n = 32) that learned using 5E LC model, XI MIA-5 class (n = 33) that learned using 5E LC+SSI, and XI MIA-6 class (n = 32) that learned using conventional method. The samples were choosen by convenience sampling technique. The test instrument consisted of 15 multiple choice items which were valid and reliable (r = 0.806). The data were analyzed using one way ANOVA test and LSD posthoc test. The results of this study indicated that the students who learned using 5E LC+SSI model showed greater levels of critical thinking skills ( = 74,95) than both the student who learned using 5E LC model ( = 74,17) and the student who learned using conventional method ( = 68,96). Based on statistics analysis, there was significant differences on students critical thinkings between students taught using conventional method and students taught either using 5E LC+SSI model and 5E LC model. However, there was no significant differences on students critical thinking skills between students taught using 5E LC+SSI model and the students taught using 5E LC model.
Pengaruh Pemberian Dosis Mineral Ca dan Lama Fermentasi Pelepah Sawit terhadap Kandungan Lignin, Kecernaan BK, BO, PK dan Fraksi Serat (NDF, ADF, Hemiselulosa dan Selulosa) menggunakan Kapang Phanerochaete chrysosporium Rahayu, S.; Jamarun, N.; Zain, M.; Febrina, D.
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 17, No 2 (2015): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.17.2.151-162.2015

Abstract

Pelepah sawit dapat dimanfaatkan sebagai pakan ternak tetapi terkendala karena tingginya kandungan lignin.  Strategi yang dapat dilakukan untuk menurunkan kandungan lignin pelepah sawit adalah melalui fermentasi menggunakan kapang Phanerochaete chrysosporium (Pc). Pertumbuhan kapang Phanerochaete chrysosporium  dipengaruhi oleh ketersediaan mineral dalam substrat. Untuk itu diperlukan penambahan mineral sesuai dengan kebutuhan kapang dimana salah satunya adalah mineral kalsium (Ca). Penelitian ini bertujuan untuk melihat pengaruh pemberian dosis mineral Ca dan lama fermentasi dalam menurunkan kandungan lignin, meningkatkan kecernaan BK, BO, PK, NDF, ADF, Selulosa dan Hemiselulosa pelepah sawit yang difermentasi menggunakan kapang Phanerochaete chrysosporium. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial untuk menghitung persentase penurunan lignin dan Rancangan Acak Kelompok (RAK) pola faktorial dengan tiga kali ulangan. Faktor A yaitu pemberian dosis mineral Ca yaitu A0: kontrol (pelepah + Pc) + 0 ppm Ca, A1: kontrol + 1000 ppm Ca, A2 : kontrol + 1500 ppm Ca, A3 : kontrol + 2000 ppm Ca. Faktor B adalah lama waktu fermentasi yaitu Y1: 10 hari, Y2 : 15 hari dan Y3 : 20 hari. Data dianalisis menggunakan analisa varian (Anova) dan untuk melihat perbedaan antar perlakuan dilakukan uji lanjut Duncan’s Multiple Range Test (DMRT) (Steel and Torrie, 1991). Hasil sidik ragam menunjukkan perlakuan memberikan pengaruh berbeda nyata (P<00.1) terhadap penurunan kandungan lignin, kecernaan BK, BO, PK, NDF, ADF, Hemiselulosa dan Selulosa. Penelitian ini dapat disimpulkan bahwa fermentasi pelepah sawit menggunakan kapang Phanerochaete chrysosporium pada dosis mineral Ca 2000 ppm dengan lama fermentasi 10 hari dapat menurunkan kandungan lignin 26,79% dengan kecernaan BK 51,44%., BO 53,24%., PK 47,54%., NDF 47,48%., ADF 42,24%., Selulosa 45,44% dan Hemiselulosa 48,34%.
Sifat Fisik Daging Domba Garut Jantan dengan Waktu Pemberian Pakan yang Berbeda Rahayu, S.; Aditya, E. L.; Jamil, S.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol 3, No 2 (2015): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.3.2.79-82

Abstract

High ambient temperature in tropical, especially during the daytime of Garut lamb cause highlevel of stress and low productivity. To overcome it can be  improved the management. If the feed is givenin the evening, its metabolism process will take place throughout the night with the lower temperature.This method is expected result the higher productivity include the meat quality. So the aim of thisstudy was to evaluate the influence of feeding time (morning and evening) toward the physical meatcharacteristics of Garut lamb. 12 Garut lamb were used. They were placed in 12 individual cages. Thisstudy used block randomized design. As treatment was feeding time. As feed was  a mix of field grass andconcentrates (40:60). Data of physical meat characteristics were analyzed by t-test. The physical meatcharacteristics include value of pH, the ability to water bind, cooking loss, and score of meat colour werenot significant. Garut lamb that was given feed at morning time, significant resulted the meat with moretender (1.04 kg/cm2) than the feeding time at evening (1.59 kg/cm2) (P<0.05). It can be concluded thatthe physical meat characteristics (except meat tenderness) of Garut lamb were similar in both differentfeeding time. The treatment of different feeding time can be change the behaviour of Garut lamb.
Komposisi jaringan karkas Domba Ekor Tipis yang diberi Pakan Hijauan pada Periode Akhir Penggemukan Baihaqi, M.; Rahayu, S.; Duldjaman, M.; Nurmalasari, Nurmalasari
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol 1, No 1 (2013): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.1.1.8-14

Abstract

The aim of this study was to evaluate the effect of different feeding system on carcass characteristics of Javanese thin-tailed sheep. Nine sheep with average body weight 15.7+1 kg were kept in individual cage and received water ad libitum. The dietary treatments was conducted in three different ratio and periode of concentrate and forage (Brachiaria humidicola) included: 80% forage and 20% concentrate for two months (P1), 20% forage and 80% concentrate for two months (P2) and 20% forage and 80% concentrate for a month followed by 80% forage and 20% concentrate for last month. Data was analysed by analyses of variance (ANOVA). The result showed that there was no significantly effect of treatment on percentage and weight of carcass, commercial cutting of carcass and meat, fat and bone of carcass. The study implicated that sheep fatted by forage for a month of last period could be done by farmer in order to minimize cost production.
Transformasi Fragmen DNA Salmonella Ke Dalam Sel E. coli DH5-α Rahayu, E.S.; Pertiwiningrum, A.; Indrati, R.; Raharjo, S.; Rahayu, S.; Margino, S.
Agritech Vol 17, No 1 (1997)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2057.365 KB) | DOI: 10.22146/agritech.19320

Abstract

Genomic DNA from Salmonella typhimarium ATCC 14028, cultured in a Luria broth for 24 hr at 37°C, was isolated from the cells using EDTA as a chelating agent and SDS as a detergent and lysing agent. The amount of genomic DNA was then digested with each of three restriction endonucleases, i.e., EcoRI, HindlII, and BamHI for 4 hr at 37°C. A plasmid (pUC19) was used as a vector. Prior to use, the pUC19 plasmid was split with one of the three restriction enzymes and dephosphorylated using a bacterial alkaline phosphatase. The genomic DNA was then ligated to the corresponding prediggested plasmid using a ligase at 15°C over night. Transformation of the DNA recombinant into E.coli DH5-a was successfully carried out using a heat shock method as indicated by gel electrophoresis