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Respons Awal Ketahanan Jagung terhadap Peronosclerospora maydis dan Induksi Bahan Kimia Habibullah, Muhammad; Widiastuti, Ani; Sumardiyono, Christanti
Jurnal Perlindungan Tanaman Indonesia Vol 22, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.26877

Abstract

Downy mildew is an important disease in maize cultivation in the world. Induced resistance is one of the methods used to control plant diseases. Reactive oxygen species (ROS) and lignification are plant defense responses. This study aims to determine the potential of chemicals as an inducer by observing ROS responses and lignification of mycelium. The materials used are benzoic acid, sodium benzoate, salicylic acid, thiamine, saccharin and aspirin with concentration 2 g L-1 of distilled water. The ROS reaction is indicated by all treatments induced and inoculated by pathogens. Lignification of mycelium occurred in the treatment of aspirin at 6 hours observation after inoculation and saccharin treatment on observation 12 hours after inoculation. Based on the observation of ROS and lignification of the mycelium, it is suspected that the material tested has the potential to be further tested as an inducer because it has the ability to activate an early marker of plant resistance in the form of ROS reaction and lignification of mycelium. IntisariBulai merupakan penyakit penting dalam budidaya jagung di dunia. Induksi ketahanan adalah salah satu metode yang digunakan untuk mengendalikan penyakit tanaman. Spesies oksigen reaktif (ROS) dan lignifikasi adalah respon pertahanan tanaman. Penelitian ini bertujuan untuk mengetahui potensi bahan kimia sebagai bahan penginduksi dengan mengamati respons ROS dan lignifikasi miselium. Bahan yang digunakan adalah asam benzoat, natrium benzoat, asam salisilat, tiamin, sakarin dan aspirin dengan konsentrasi 2 g akuades L-1. Reaksi ROS ditunjukkan oleh semua perlakuan yang diinduksi dan diinokulasi oleh patogen. Lignifikasi miselium terjadi pada perlakuan aspirin pada pengamatan 6 jam setelah inokulasi dan perlakuan sakarin pada pengamatan 12 jam setelah inokulasi. Berdasarkan pengamatan ROS dan lignifikasi miselium, diduga bahwa bahan yang diuji memiliki potensi untuk diuji lebih lanjut sebagai bahan penginduksi karena memiliki kemampuan untuk mengaktifkan penanda awal ketahanan tanaman dalam bentuk reaksi ROS dan lignifikasi miselium.
Evaluation of Some Specific Primer Sets Development for Detecting Fusarium oxysporum f. sp. cubense Tropic Race 4 (Foc TR4) Originating from Indonesia Pratama, Yudha; Wibowo, Arif; Widiastuti, Ani; Subandiyah, Siti; Widinugraheni, Sri; Rep, Martijn
Jurnal Perlindungan Tanaman Indonesia Vol 22, No 1 (2018): (In Press)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.25037

Abstract

Fusarium oxysporum f. sp. cubense tropic race 4 (Foc TR4) strain which belong to Vegetative Compatibility Group (VCG) 01213 is the most devastating disease in global banana production. Validation of specific primer sets using the positive control (Foc TR4). In total, 50 isolates of Foc are collected from several banana production regions in Indonesia represent the group of VCG, races, genotype, cultivars, which are confirmed as Foc based on the tested using FocEf3 primer set, except Cjr-2 and Lmp-4 isolates. Foc-1/Foc-2 could amplify 34 Foc isolates included in Foc race 4. Three specific primer sets i.e. TR4-F/TR4-R, Six-1c, and TR4-F2/TR4-R1 are used to classify Foc isolates into Foc tropic race 4. TR4-F/TR4-R is known have the highest specificity as it could amplify 35 Foc isolates including positive controls (Foc TR4) compared to the other primer sets (Six-1c and TR4-F2/TR4-R1). This research indicates that there are a large number of diversity strains found in Foc isolates to be studied for further research. Race 4 of Foc (STR4 or TR4) is known to be widespread in several regions in Indonesia. Therefore, specific primer set development needs to be done to detect Foc TR4 and the most damaging strains on Foc TR4 based on molecular data.IntisariFusarium oxysporum f. sp. cubense ras 4 tropika (Foc TR4) yang termasuk ke dalam kelompok VCG 01213 merupakan patogen yang paling merusak dalam produksi tanaman pisang secara global. Validasi primer spesifik berbasis PCR menggunakan kontrol positif (Foc TR4). Total, 50 isolat Foc dikoleksi dari  beberapa daerah produksi pisang di Indonesia mewakili VCG, ras, genotipe dan kultivar yang dikonfirmasi sebagai isolat Foc berdasarkan pengujian menggunakan primer FocEf3, kecuali isolat Cjr-2 dan Lmp-4. Foc-1/Foc-2 dapat mengamplifikasi 34 isolat Foc yang termasuk ke dalam Foc ras 4. Selanjutnya tiga pasang primer spesifik yaitu TR4-F/TR4-R, Six-1c, dan TR4-F2/TR4-R1 digunakan untuk mengelompokkan isolat-isolat tersebut ke dalam isolat Foc ras 4 tropika. TR4-F/TR4-R diketahui memiliki spesifitas tertinggi karena dapat mengamplifikasi sebanyak 35 isolat Foc termasuk kontrol positif (Foc TR4) dibandingkan dengan primer lainnya (Six-1c dan TR4-F2/TR4-R1). Penelitian ini menunjukkan bahwa terdapat sejumlah besar keragaman strain yang terlihat pada isolat-isolat Foc tersebut untuk dapat dipelajari lebih lanjut. Ras 4 dari Foc (STR4 atau TR4) diketahui tersebar luas pada beberapa daerah di Indonesia. Oleh karena itu, perlu dilakukan pengembangan primer spesifik untuk mendeteksi Foc TR4 dan strain yang paling merusak pada Foc TR4 berdasarkan data molekuler.
Pengaruh Perlakuan Uap Air Panas dengan Sistem Pemanasan Terbuka terhadap Kesehatan dan Viabilitas Benih Jagung Kurniawan, Singgih; Widiastuti, Ani; Maryudani, Y. M. S.
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11884

Abstract

The existence of seed-borne fungi could degrade the seed quality in its viability and may cause disease after planting. The aim of this research was to know the hot vapor treatment effectiveness in controlling that fungal disease and its influence to the viability of corn seed. The treatment effectiveness was measured based on the reduction of the fungal growth and sporulation on Potato DextroseAgar (PDA), the fungal infection on seed, and not reduced the seed viability significantly after treatment. Hot vapor treatment was done in 50°C, 60°C, and 70°C temperature for 20 minutes and a control. The temperature treatments cover 5 minutes of antecedent treatment in the form of appliance warm-up until the target temperature obtained, 10 minutes in the treatment drum and 5 minutes for resting time while the faucet is being shut down but the exhaust fan remain to be opened. Result of the research show that in vitro test of hot water vapor on 60oC and 70oC killed Aspergillus flavus, A. niger, Fusarium sp., and Penicillium sp. isolates. Both of the temperatures reduced the corn seed viability significantly. Infection of A. flavus still dominant in corn seed after treated on the three temperatures.The hot vapor treatment with 50oC is good for seed treatment of 408.9 g corn seed although the seed had been stored for about 9 months. Adanya jamur terbawa benih dapat menurunkan viabilitas dan kemungkinan dapat menimbulkan penyakit setelah benih ditanam di lapangan. Penelitian ini bertujuan untuk mengetahui keefektifan perlakuan uap air panas untuk mengendalikan jamur terbawa benih dan pengaruhnya terhadap viabilitas benih jagung. Keefektifan perlakuan diukur berdasarkan kekuatan mereduksi pertumbuhan koloni dan sporulasi jamur yang ditumbuhkan pada medium Potato Dextrose Agar (PDA), tingkat infeksi jamur pada biji dan tidak menurunkan viabilitas biji. Perlakuan ini menggunakan suhu 50o C, 60o C, dan 70o C selama 20 menit serta tanpa perlakuan sebagai kontrol. Lama perlakuan meliputi 5 menit perlakuan pendahuluan berupa pemanasan awal sampai suhu yang diinginkan, 10 menit untuk pemanasan drum perlakuan, dan 5 menit waktu istirahat yaitu kran uap air dimatikan tetapi kipas exhaust tetap dihidupkan. Hasil penelitian in vitro menunjukkan bahwa perlakuan uap air panas bersuhu 60o C dan 70o C mematikan isolat Aspergillus flavus, A. niger, Fusarium sp. dan Penicillium sp. Kedua suhu tersebut menurunkan viabilitas benih jagung secara nyata. Infeksi A. flavus masih dominan dalam benih jagung walaupun telah diperlakukan dengan ketiga macam suhu uap air panas. Perlakuan uap air panas bersuhu 50o C pada benih jagung sebanyak 408,9 g dapat dipilih sebagai perlakuan yang baik untuk benih jagung walaupun telah disimpan sekitar 9 bulan
Penyakit-Penyakit Penting Buah Naga di Tiga Sentra Pertanaman di Jawa Tengah Wibowo, Arif; Widiastuti, Ani; Agustina, Wahyu
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3066.04 KB) | DOI: 10.22146/jpti.9816

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The objective of this study was to identify the causal agent of some dragon fruit disease emerging in Daerah Istimewa Yogyakarta (DIY) and Central Java. Samples were taken from the dragon fruit plantation from the district of Sleman and Kulonprogo, Province of Daerah Istimewa Yogyakarta as well as Magelang, Province of Central Java. Isolation of pathogen from symptomatic plant tissue was performed on Potato Dextrose Agar (PDA) for fungi and Nutrient Agar (NA) for bacteria and continued with Kochs postulates testing. The results of field observation showed that the disease commonly occured in all 3 plantations of dragon fruit were stem rot caused by Erwinia sp. and scab caused by Pestalotiopsis sp. Other miscellaneous diseases found among the plantations were brown spot (Fusarium sp.), anthracnose (Colletotrichum sp.), mosaic that might be caused by Cactus Virus X, root knotnematode (Meloidogyne sp.), black rot and red spot which were still unidentified.Penelitian ini bertujuan untuk mengetahui penyakit-penyait penting pada tanaman buah naga yang ditanam pada sentra pertanaman buah naga di Propinsi Daerah Istimewa Yogyakarta (DIY) dan Jawa Tengah, serta untuk mengidentifikasi penyebab penyakit penting tersebut. Sampel tanaman buah naga diambil dari pertanaman buah naga di Kabupaten Sleman dan Kulon Progo untuk Provinsi DIY serta Magelang untuk Propinsi Jawa Tengah. Isolasi patogen dari jaringan tanaman bergejala dilakukan pada medium Potato Dextrose Agar (PDA) untuk jamur dan Nutrient Agar (NA) untuk bakteri serta dilanjutkan dengan uji Postulat Koch. Hasil pengamatan di lapangan menunjukkan bahwa penyakit yang umum terdapat di 3 lokasi pertanaman buah naga tersebut adalah busuk batang yang disebabkan oleh Erwinia sp. dan kudis yang disebabkan oleh Pestalotiopsis sp. Adapun penyakit-penyakit lain yang dijumpai antara lain bercak coklat (Fusarium sp.), antraknosa (Colletotrichum sp.), mosaik yang kemungkinan disebabkan oleh Cactus Virus X, puru akar (Meloidogyne sp.), serta busuk hitam dan bercak merah yang belum teridentifikasi penyebabnya.
Uji Efektivitas Pestisida terhadap Beberapa Patogen Penyebab Penyakit Penting pada Buah Naga (Hylocereus sp.) secara In Vitro Widiastuti, Ani; Agustina, Wahyu; Wibowo, Arif; Sumardiyono, Christanti
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.825 KB) | DOI: 10.22146/jpti.9828

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Problem caused by pathogen in Dragon Fruit (Hylocereus sp.) cultivation became very important because it decreased significantly the quantity and quality of the fruit production. This study aims to determine the effectiveness of some pesticides against pathogens that cause diseases of dragon fruit in several plantations in the DIY (Sleman and Kulon Progo) and Central Java (Magelang and Batang). Test of fungicide effectiveness was done in vitro on PDA medium (potato dextrose agar) by poisoned food technique. The fungicides were mancozeb 80 %, methyl tiofanat 70 %, copper hydroxide 80 %, chlorotalonil 75 %, mancozeb 64 % + metalaxyl 4 %, mancozeb karbendazim 73.8 % + 6.8 %,benomyl 50 % at a concentration of 1g/L. The bactericides used were streptomycin sulfate 20 % and oxytetracycline 150 AL with each concentration of 1 g/L and 1 mL /L. The results showed that benomyl 50 % was the most effective fungicide to suppress the growth of Fusarium sp. (brown spot), Colletotrichum sp. (anthracnose) and Pestalotiopsis sp. (scab), followed by mancozeb 73.8 % +karbendazim 6.8 % and 73.8 % mancozeb. Bactericide which was able to suppress the growth of Erwinia sp. (stem rot) was streptomycin sulfate 20 %. Gangguan patogen pada buah naga (Hylocereus sp.) saat ini menjadi masalah penting karena secara signifikan menurunkan kuantitas dan kualitas hasil panen. Penelitian ini bertujuan untuk mengetahui keefektivan beberapa pestisida terhadap patogen penyebab penyakit-penyakit penting pada tanaman buah naga yang ditanaman pada beberapa sentra pertanaman di Propinsi DIY (Sleman dan Kulon Progo) dan Jawa Tengah (Magelang dan Batang). Uji keefektivan pestisida dilakukan secara in vitro pada medium PDA (potato dextrose agar) dengan metode teknik makanan beracun (poisoned food technique). Fungisida yang dipergunakan adalah mankozeb 80%, tiofanat metil 70%, tembaga hidroksida 80%, klorotalonil 75%, mankozeb 64% + metalaksil 4%, mankozeb 73,8% + karbendazim 6,8%, benomil 50% dengan konsentrasi 1g/L, sedangkan bakterisida yang dipergunakan adalah streptomisin sulfat 20% dan oksitetrasiklin 150 AL dengan konsentrasi masing-masing 1 g/L dan 1 mL/L. Hasil penelitian menunjukkan bahwa dari enam jenis fungisida yang dipergunakan, benomil 50% merupakan fungisida yang paling efektif untuk menekan pertumbuhan Fusarium sp. (bercak cokelat), Colletotrichum sp. (antraknos), dan Pestalotiopsis sp. (kudis), diikuti oleh mankozeb 73,8% + karbendazim 6,8% serta mankozeb 73,8%. Untuk fungisida yang lain, efektifitasnya tidak terlalu tinggi jika dibandingkan dengan perlakuan kontrol. Bakterisida yang mampu menekan perkembangan Erwinia sp. (busuk batang) adalah streptomisin sulfat 20%.
PENYAKIT BUSUK AKAR DAN PANGKAL BATANG TEBU DI SUMATERA SELATAN Maryono, Tri; Widiastuti, Ani; Priyatmojo, Achmadi
Jurnal Fitopatologi Indonesia Vol 13 No 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.321 KB) | DOI: 10.14692/jfi.13.2.67–71

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Root and basal stem rot disease caused by Xylaria sp. is a new diseases in Indonesian sugarcane plantation. The disease has been reported only occurred in sugarcane plantations in Sumatera. Limited information about the disease has slowed down disease diagnosis in the field. The research was aimed to describe symptoms, signs, and spatial distribution of the disease caused by Xylaria. A survey was conducted at a sugarcane plantation in South Sumatera (3° 26? 16.6? S dan 104°40? 09.8? E). Typical symptoms of the disease involved drying of all leaves and plant death, dry rot of root and basal stem, and retarded growth of ratoon cane were recorded. Stroma was found on surface of diseased stems and on soil surface of diseased plants, and spatial aggregation of diseased plants was observed in all locations. The distribution of the diseased plant aggregately and the presence of stroma on diseased plants are important characteristics of the disease diagnosis in sugarcane plantations.
IDENTIFIKASI CENDAWAN PENYEBAB PENYAKIT PASCAPANEN PADA BEBERAPA BUAH DI YOGYAKARTA Widiastuti, Ani; Ningtyas, Ovianne Hapsari; Priyatmojo, Achmadi
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.361 KB) | DOI: 10.14692/jfi.11.3.91

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In Indonesia, high yield losses due to post-harvest diseases are often difficult to measure because research focusing on such matter are still limited. This study aimed to determine the genera of fungi that cause rot on postharvest fruit, which can be used as a basis to determine the important pathogens in the current post-harvest commodities and for further disease management. The method used is sample collection, single spore isolation, microscopic observation and identification of fungal genera. Colletotrichum sp. was sucessfuly isolated from antrachnose of papaya. Pestalotia sp. was found in the fruit rot of avocado and star fruit. Lasiodiplodia sp. was found in mango, while Pestalotia sp. and Lasiodiplodia sp. was found in both sapodilla and banana. Alternaria sp. was found in the fruit rot of pears and apples. Aspergillus sp. was found in grapes, and Fusarium sp. was isolated from pineapple fruit rot.
Intensitas Cemaran Jamur pada Biji Jagung Pakan Ternak Selama Periode Penyimpanan Puspitasari, Destania Putri Indah; Widiastuti, Ani; Wibowo, Arif; Priyatmojo, Achmadi
Jurnal Perlindungan Tanaman Indonesia Vol 19, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.021 KB) | DOI: 10.22146/jpti.16066

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This research aimed to know the intensity of fungal contamination in maize grain cattle-feed during storage. Five kilogram of grain sample were collected from Klaten, Sleman, and Muntilan, then stored at CV. Ragil Jaya’s warehouse for two months. Every two weeks the water content were measured and the grain were tested using PDA and blotter methods. Incubation during isolation process were conducted for seven days at 12 hour darkness and 12 hour light. Results showed that dominant fungal contamination from Klaten, Sleman, and Muntilan wasAspergillus sp. As2 isolate with contamination intensity as much as 89% (blotter), 73% (PDA), and 44% (blotter). The results also showed that factors which influenced the intensity of fungal contamination in cattle-feed maize is the grain condition before storage such as broken grain, dirt, and insect; and not caused by the planting location.  INTISARIPenelitian ini bertujuan untuk mengetahui intensitas cemaran jamur dominan pada biji jagung yang digunakan sebagai pakan ternak selama penyimpanan. Biji jagung pakan ternak dari Klaten, Sleman, dan Muntilan sebanyak 5 kg disimpan di gudang CV. Ragil Jaya, Magelang selama 2 bulan. Pengukuran kadar air dilakukan setiap 2 minggu dan kemudian diuji dengan metode PDA dan blotter untuk mengetahui cemaran jamur pada biji jagung. Hasil penelitian ini menunjukkan bahwa dari sampel biji jagung pakan ternak yang berasal dari Klaten, Sleman, dan Muntilan, jamur cemaran yang mendominasi, yaitu Aspergillus sp. isolat As2 dengan intensitas cemaran jamur tertinggi di daerah Klaten 89% (blotter), Sleman 73% (PDA), dan Muntilan 44% (blotter). Hasil ini menunjukkan bahwa hal yang mempengaruhi intensitas cemaran jamur pada jagung pakan ternak adalah kondisi awal bahan yang disimpan yaitu ada tidaknya kerusakan, kotoran, dan serangga; bukan lokasi penanaman jagung.
Pengaruh Stomata dan Klorofil pada Ketahanan Beberapa Varietas Jagung terhadap Penyakit Bulai Agustamia, Christine; Widiastuti, Ani; Sumardiyono, Christanti
Jurnal Perlindungan Tanaman Indonesia Vol 20, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.17703

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Resistant varieties are more advisable for controlling maize downy mildew compared with fungicides which is not effective and not environmentally friendly. This study is aimed to determine resistance of some varieties of maize against downy mildew. The maize varieties used were BS 0114, BS 0214, BS 0314, PAC 105, Sweet Corn and BISI 2. The parameters measured were disease incidence and intensity, chlorophyll content of leaves, stomatal density and plants dry weight. Data were analyzed by using analysis of variance (ANOVA) and Duncans Multiple Range Test (DMRT). The results indicated that PAC 105, BS 0214 and BS 0314 were resistant varieties, while BS 0114, Sweet Corn and BISI 2 were susceptible. PAC 105 variety has the lowest stomatal density (65.353/mm2), and Sweet Corn variety has the highest stomatal density (110.79/mm2). Stomatal density was positively correlated with the disease intensity. Higher disease intensity has lower chlorophyll content compared with the lower intensity. PAC 105 variety has the highest chlorophyll content and plant dry weight, while Sweet Corn variety has the lowest chlorophyll content and plant dry weight. INTISARI Penggunaan varietas tahan bulai lebih dianjurkan digunakan dalam pengendalian penyakit bulai pada jagung dibandingkan dengan penggunaan fungisida karena tidak efektif dan tidak ramah lingkungan. Penelitian ini bertujuan untuk mengetahui ketahanan beberapa varietas jagung terhadap penyakit bulai. Varietas yang digunakan meliputi BS 0114, BS 0214, BS 0314, PAC 105, jagung manis, dan BISI 2. Parameter yang diamati adalah insidensi dan intensitas penyakit, kandungan klorofil setelah inokulasi, kerapatan stomata dan berat kering tanaman. Data yang diperoleh diuji dengan analisis varians (ANOVA) dan uji lanjut dengan Duncan’s Multiple Range Test (DMRT). Hasil penelitian rumah kaca menunjukkan bahwa varietas PAC 105, BS 0214 dan BS 0314 merupakan varietas tahan, sedangkan varietas BS 0114, jagung manis dan BISI 2 merupakan varietas yang rentan. Varietas tahan PAC 105 memiliki kerapatan stomata paling rendah yaitu 65,353/mm2, dan kerapatan stomata paling tinggi dimiliki oleh varietas rentan yaitu jagung manis dengan kerapatan 110,79/mm2. Kerapatan stomata berkorelasi positif dengan intensitas penyakit dengan koefisien korelasi (r) sebesar 0,72526. Semakin rendah intensitas penyakit bulai kandungan klorofil dan berat kering yang semakin tinggi. Varietas PAC 105 yang mempunyai intensitas penyakit paling rendah , mempunyai kandungan klorofil dan berat kering tertinggi, sedangkan varietas jagung manis mempunyai kandungan klorofil dan berat kering terendah.
Pendampingan Petani Stroberi di Desa Serang, Karangreja, Purbalingga, Jawa Tengah Dalam Manajemen Kesehatan Tanaman Melalui KKN-PPM Universitas Gadjah Mada: Perlunya Bibit Stroberi Sehat dan Peningkatan Keterlibatan Petani dalam Kegiatan Kelompok Widiastuti, Ani
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.16953

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Serang village, Karangreja, Purbalingga is a plateau area with an altitude of about 650—1300 above sea level, has high rain fall around 6,240 mm with an average temperature of 20°C. This village is a leading commodity for strawberry with plant area has ever reached 64ha. The main problem faced by the strawberries farmers in the village of Serang is a problem of plant health management to support the production, especially on rainy season. Awareness about healthy seedlings as a main aspect of plant health managed mental so needs to be improved. Therefore, a collaborative research and empowerment of farmers is arranged to assist farmers in identifying problems and how to solve by their own that in maintaining the health of the strawberry plant. This project was conducted firstly in 2013 by KKN-PPM UGM students in collaboration with Ibaraki University, Japan, Bappeda Purbalingga, and government of Purbalingga Regency. The methods were direct survey of field, farmers interview, extension and discussion among students and farmers in formal and informal meetings, then continued by some trainings. The informal meetings were conducted on site in farmers field and it was assumed that although it was not really efficient in time and energy, but it was effective for farmers to emerge their idea on identifying problems and solving methods therefore the farmers empowerment was done in this matter. Due to problem identification activities, it was known that healthy seedlings were the main problem in strawberry cultivation there. The involvement of farmers in the group also needs to be improved as a forum for exchange of ideas to move forward together. As this activity in 2013 is the first project of long-term project to contribute for Serang farmers in plant health management. Some creative methods need to be designed and improved in the future.