Henna Ria Sunoko, Henna Ria
Bagian Farmasi Rumah Sakit Dr. Kariadi/Universitas Diponegoro Semarang

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RICE LEAF EXTRACT FOR KIDNEY DAMAGE PREVENTION IN PLUMBUM-EXPOSED RATS Santosa, Budi; Sunoko, Henna Ria; Sukeksi, Andri
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Abstract. Plumbum (Pb) is a heavy metal affecting renal tubular epithelial cells and causing necrosis. This study aimedto demonstrate the effectiveness of rice leaf extract in degeneration and necrosis prevention of renal tubule epitheliumexposed to lead. Twenty eight rats were divided into a control and 3 treatment groups all exposed to Pb by 0.5 g/ kg/ day,but only the treatment groups had received rice leaf extract in varied doses for 8 weeks. Results from Kruskal-Wallis andsphearman rho tests showed that the number of normal cells, degeneration and necrosis were significantly different (0.03;0.05; 0.04) between control and treatment groups with a strong positive correlation of treatment (r = 0.97) in normal cellsand a strong negative one in cell degeneration and necrosis. (R = -0.92 and r = -0.93). As conclusion, rice leaf extractshowed ability in the prevention of kidney damage in rats exposed to lead.
ANALYSIS, IDENTIFICATION, AND FORMULATION OF METALLOTHIONEIN EXTRACTS ON NUMEROUS VARIETIES OF PADDY 95 LEAVES Santosa, Budi; Sunoko, Henna Ria
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Background: Vegetable materials, such as rice, corn, bean, and soybean have metallothionein proteins contained in their roots, stems, leaves, flowers, and fruits. Of those vegetable materials, paddy leaves have the most numerous metallotionein proteins. However, the varieties which produce the highest metallotionein proteins have not yet been found. The roles of metallothionein proteins are to bind and to detoxify heavy metals. Objectives:  to analyze, identify, and formulate the metallothionein extractscontained in numerous varieties of paddy leaves. An experimental research method. Each vegetable material of numerous varieties of paddy leaves is made into simplicia and is then processed into infusion. ELISA method is used as a technique to measure the protein level of each infusion. The metallothionein protein levels contained in numerous varieties of paddy leaves are then identified to determine the one with the highest metallothionein protein level. Extract formula is taken from the highest metallothionein level. Results :the highest metallothionein protein level is contained in paddy leaves of IR begendit (28.832 ng) while the lowest one is in paddy leaves of IR-64 SS (0.358 ng). The extracts of Paddy leaves of IR begendit with the highest protein level are then formulated into metallothionein extracts. Conclusion: Analysis on metallothionein levels of numerous varieties of paddy leaves with the highest protein level is contained in paddy leaves of IR begendit (28.832 ng) while the lowest one is in paddy leaves of IR-64 SS (0. 358 ng).Keywords: paddy leaves, metallothionein
EKSTRAK AIR DAUN PADI MEMPERBAIKI HEMATOPOESIS PADA TIKUS YANG TERPAJAN PLUMBUM Santosa, Budi; Sunoko, Henna Ria; Sukeksi, Andri
Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Plumbum (Pb) asetat menyebabkan gangguan hematopoesis melalui hambatan biosintesis heme dan defisiensi enzim G-6PD yang menimbulkan penurunan hemoglobin dan jumlah eritrosit, serta peningkatan sel retikulosit. Ekstrak daun padi mengandung protein metallothionein yang mengikat Pb. Tujuan penelitian adalah membuktikan ekstrak daun padi memperbaiki gangguan hematopoesis pada tikus terpajan Pb. Metode penelitian adalah randomized post test only control-group design, 28 tikus dibagi ke dalam 4 kelompok yaitu 1 kelompok kontrol dan 3 kelompok perlakuan. Kelompok kontrol dan perlakuan dipajan Pb 0,5 g/kgBB/hari dan kelompok perlakuan diberi ekstrak daun padi 0,2; 0,4; 0,8 setiap hari melalui sonde sampai minggu ke-8. Hari terakhir  minggu ke-8 diperiksa jumlah kadar Hb menggunakan cyanmethemoglobin dan jumlah eritrosit diukur menggunakan haematology analyzer, serta retikulosit menggunakan pengecatan BCB. Tempat penelitian dilaksanakan di LPPT UGM dan laboratorium biomedik UNIMUS Semarang, Maret?Mei 2014. Perbedaan antarkelompok untuk kadar Hb dan jumlah eritrosit dilakukan uji one way enzyme linked immunosorbent assays (ANOVA) dan Kruskal-Wallis untuk jumlah retikulosit. Hasil penelitian menunjukkan kadar Hb rata-rata mengalami kenaikan tidak bermakna (p=0.81), jumlah eritrosit rata-rata tidak ada perbedaan antara kelompok kontrol dan perlakuan (p=0,83), jumlah retikulosit rata-rata menurun bermakna dari kelompok kontrol sampai perlakuan (p=0,00). Simpulan, ekstrak daun padi mampu memperbaiki hematopoesis pada tikus terpajan Pb. [MKB. 2015;47(2):84?90]Kata kunci: Ekstrak daun padi, hematopoesis, pajanan Pb, tikusRice Leaf Water Extract Improves Hematopoiesis in Lead-Exposed Rats  Abstract Plumbum ( Pb ) acetate leads to hematopoiesis disruption by inhibiting heme biosynthesis and creating G-6PD enzyme deficiency, leading to decreased hemoglobin concentration and red cell count as well as  increased reticulocyte cells. Rice leaf extract contains metallothionein proteins that bind Pb. This study aimed to prove the ability of rice leaf extract wa to improve hematopoiesis disorder conditions in rats exposed to Pb. The method used was the randomized post-test only control group design. Twenty eight rats were divided into 4 groups: 1 control group and 3 treatment groups . In the control group and the treatments groups, the experimental rats were treated with 0.5 g/kg/day Pb. The treatment groups were treated by 0.2, 0.4, and 0.8 rice leaf extract, respectively, every day for 8 weeks. On the last day of the 8th week, blood samples were collected for Hb concentration measurements using cyanmethemoglobin and the erythrocytes and reticulocytes number were counted using a hematology analyzer and BCB, respectively. This study was conducted in LPPT UGM and Biomedicine UNIMUS in March?May 2014. The differences between groups in hemoglobin and erythrocyte parameters were tasted  using one way ANOVA while  Kruskal-Wallis test was used for the reticulocyte count. The results show that rice leaf extract treatment to rats exposed to Pb did not affect Hb (p=0.81) and erythrocytes count  (p=0.83), However, the mean reticulocyte count decreased significantly in Pb-exposed rats treated with rice leaf extract (p=0.00). In conclusion, rice leaf extract is able to improve hematopoiesis in rats exposed to Pb. [MKB. 2015;47(2):84?90]Key words: Hematopoiesis, Pb, rice leaf extract, rat DOI: 10.15395/mkb.v47n2.458
Aqueous IR Bagendit rice leaf extract decreases reticulocyte count in lead-exposed rats Santosa, Budi; Sunoko, Henna Ria; Sukeksi, Andri
Universa Medicina Vol 37, No 1 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2018.v37.57-64

Abstract

BACKGROUNDLead acetate may inhibit the enzyme aminolevulinate dehydratase (ALAD) resulting in decreased heme synthesis (and consequently in anemia) but in increased number of reticulocyte cells. IR Bagendit paddy leaf water extract has a high metallothionein protein content which acts to bind to lead. The study objective was to determine whether aqueous IR Bagendit rice leaf extract dosage variations prior to lead exposure decreases reticulocyte count in lead-exposed rats. METHODSThe study was of randomized post test only control-group design involving a sample of 28 rats, that were randomized into 4 groups consisting of 1 control group and 3 treatment groups, daily administered with aqueous IR Bagendit rice leaf extract of respectively 0.2; 0.4; 0.8 mg using a feeding tube up to week 13. Lead exposure was also given using a feeding tube to both control and treatment groups at a dose of 0.5 g/kg BW/day, up to week 13. The reticulocyte count was then examined using supravital brilliant cresyl blue staining. The reticulocyte count was determined per 1000 erythrocytes and then converted into a percentage. Kruskal Wallis test followed with Bonferroni test was conducted to figure out the differences between groups. RESULTSMean reticulocyte count decreased significantly, starting from the control group up to the third treatment group (15.48 ± 3.41; 12.25 ± 03.28; 10.45 ±1.47; 9.10 ± 2.35 average per unit) (p=0.02). The Bonferroni test showed that the reticulocyte count was significantly decreased in the third treatment group (p=0.004). CONCLUSIONAqueous rice leaf extract significantly decreases reticulocytes in rats exposed to lead.