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Pharmacogenomics and Pharmacogenetics: Some Cases in Oncology Yuliwulandari, Rika
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.773 KB) | DOI: 10.19106/JMedScieSup004804201627

Abstract

ABSTRACTPharmacogenomics and pharmacogenetics plays an important role in understanding how genetic variants influence drug efficacy and toxicity.  In the case of cancer, both efficacy and toxicity of therapeutic agents determine the improvement of survival and quality of life in cancer patients.  Effective treatment of Cancer, one of the most deadly diseases in the world, is importance for extending patient survival. Genetic variation influences the response of an individual to drug treatments that impact the efficacy and toxicity of the drugs. Understanding individual genetic variation is potential to make therapy safer and more effective by determining more appropriate drug selection and drug dosage for each individual patient. In the context of cancer, tumors may have specific disease-defining mutations, but a patient’s germline genetic variation will also affect drug response. Advance research technologies approach such as GWAS and next-generation sequencing technologies, statistical genetics analysis methods and clinical trial design have shown promise for discovery of genetic variants associated with drug response in cancer. Understanding the molecular characteristics of both the tumor and the patient, and establishing their relation with drug outcomes will be critical for the identification of predictive biomarkers and to provide the basis for individualized treatments. Since cancer is also frequent in Indonesia, pharmacogenomics study in oncology and its implementation in the clinical practice is important. Broad collaboration at national and international level could foster the application of personalized medicine in oncology.
Media Edukasi Berbasis Cetak dan Digital Tentang Pengobatan Lepra dan Efek Samping Obat Mempermudah Pemahaman Penderita Lepra, Keluarga, Masyarakat dan Tenaga Kesehatan di Tanjung Pasir Tentang Lepra dan Terapinya Yuliwulandari, Rika; Zahroh, Hilyatuz; Susilowati, Retno Wilujeng; Hendri, Faradyna; Usman, Herman
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 3, No 1 (2017): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.26288

Abstract

Leprosy can be cured with multi-drug therapy as recommended by WHO. However, the presence of leprosy stigma prevents people from obtaining early detection and proper treatment. Furthermore, the adverse effect of leprosy treatment on certain individuals with genetic susceptibility, known as dapsone hypersensitivity syndrome, is often presumed as a malpractice. This further lowered people willingness to seek medical help. The reluctance to cure the disease could worsen the disease condition and spread it to their community. To overcome these problems, there should be efforts to disseminate medical information to community which discuss about leprosy treatment and its possible adverse effect. In this activity we, together with Puskesmas Desa Tegal Angus, informed and educated leprosy patients, their family and their neighbouring community with the help of educational media in the form of print and digital media. The aim of this activity was to share correct information on leprosy and the urgency of leprosy treatment, which in turn would reduce leprosy stigma and increase the patients’ willingness to complete their full course of treatment. According to the test result, people gained improved understanding about leprosy, especially in term of its definition, its transmission and treatment, factors that influence its susceptibility (including genetic factor) and how to avoid deformity due to leprosy.
Media Edukasi Berbasis Cetak dan Digital Tentang Pengobatan Lepra dan Efek Samping Obat Mempermudah Pemahaman Penderita Lepra, Keluarga, Masyarakat dan Tenaga Kesehatan di Tanjung Pasir Tentang Lepra dan Terapinya Yuliwulandari, Rika; Zahroh, Hilyatuz; Susilowati, Retno Wilujeng; Hendri, Faradyna; Usman, Herman
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 3, No 1 (2017): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (756.371 KB) | DOI: 10.22146/jpkm.26288

Abstract

Leprosy can be cured with multi-drug therapy as recommended by WHO. However, the presence of leprosy stigma prevents people from obtaining early detection and proper treatment. Furthermore, the adverse effect of leprosy treatment on certain individuals with genetic susceptibility, known as dapsone hypersensitivity syndrome, is often presumed as a malpractice. This further lowered people willingness to seek medical help. The reluctance to cure the disease could worsen the disease condition and spread it to their community. To overcome these problems, there should be efforts to disseminate medical information to community which discuss about leprosy treatment and its possible adverse effect. In this activity we, together with Puskesmas Desa Tegal Angus, informed and educated leprosy patients, their family and their neighbouring community with the help of educational media in the form of print and digital media. The aim of this activity was to share correct information on leprosy and the urgency of leprosy treatment, which in turn would reduce leprosy stigma and increase the patients’ willingness to complete their full course of treatment. According to the test result, people gained improved understanding about leprosy, especially in term of its definition, its transmission and treatment, factors that influence its susceptibility (including genetic factor) and how to avoid deformity due to leprosy.
High frequency of NAT2 slow acetylator alleles in the Malay population of Indonesia: an awareness to the anti-tuberculosis drug induced liver injury and cancer Susilowati, Retno W.; Prayuni, Kinasih; Razari, Intan; Bahri, Syukrini; Yuliwulandari, Rika
Medical Journal of Indonesia Vol 26, No 1 (2017): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.229 KB) | DOI: 10.13181/mji.v26i1.1563

Abstract

Background: Arylamine N-acetyltransferase 2 (NAT2) polymorphism was previously reported to have association with the risk of drug toxicities and the development of various diseases. Previous research on the Indonesian population, especially Javanese and Sundanese, showed that there were 33% NAT2 slow acetylator phenotype. The aim of this study was to map the NAT2 variation in the Malay ethnic to gain a deeper insight into NAT2 haplotypic composition in this ethnic.Methods: 50 healthy samples from the Indonesian Malay ethnic were obtained. They were interviewed about their ethnic backgrounds for the last three generations. DNA was extracted from peripheral blood and NAT2 genotyping was done using the PCR direct Sequencing. Data were compiled according to the genotype and allele frequencies estimated from the observed numbers of each specific allele. Haplotype reconstruction was performed using PHASE v2.1.1 software.Results: We found 7 haplotypes consisting of 6 SNPs and 14 NAT2 genotype variations in Indonesian Malay population. The most frequent allele was NAT2*6A (38%) which was classified as a slow acetylator allele. According to bimodal distribution, the predicted phenotype of the Malay population was composed of 62% rapid acetylator and 38% slow acetylator. According to trimodal distribution, the predicted phenotypes for rapid, intermediate and slow acetylators were 10%, 52% and 38% respectively.Conclusion: Our result indicates the presence of the allelic distribution and revealed the most frequent acetylator status and phenotype for the Indonesian Malay population. The result of this study will be helpful for future epidemiological or clinical studies and for understanding the genetic basis of acetylation polymorphism in Indonesia.
THE RISK FACTORS FOR DRUG INDUCED HEPATITIS IN PULMONARY TUBERCULOSIS PATIENTS IN DR. SOETOMO HOSPITAL Soedarsono, Soedarsono; Mandayani, Sari; Prayuni, Kinasih; Yuliwulandari, Rika
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 3 (2018)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.097 KB) | DOI: 10.20473/ijtid.v7i3.8689

Abstract

Tuberculosis (TB) is still a major public health problem in Indonesia. Anti-tuberculosis drug-induced hepatotoxicity (DIH) is common side effect leading to changes in treatment regimens, and the less effective second-line treatments. Several risk factors such as age, sex, body mass index (BMI) and acetylization status for hepatotoxicity were suggested in previous studies but in the fact, those are often not related to DIH incidence after receiving standard TB treatment regimen. The aim of this study was to asses the role of risk factors in the DIH incidence in pulmonary TB patients receiving standard TB treatment regimen in Dr. Soetomo Hospital, Surabaya. Study design was analytic observational with case control. The subjects were 30 TB DIH patients and 31 TB non-DIH patients receiving standard national TB program therapy. DIH severity was divided based on International DIH Expert Working Group. Demographic data and BMI status were taken from medical records. The age classification are ≥35 years old and <35 years old as one of the risk factors studied. DNA sequencing was used to assess single-nucleotide polymorphisms in NAT2 coding region to evaluate acetylator status from blood samples. The risk factors were evaluated using chi-square test and Mantel-Haenszel test. Significant association between low BMI and DIH in general was identified (OR=3.017; 95% CI=1.029-8.845) and more significant association between low BMI and moderate DIH (OR=15.833; 95% CI=1.792-139.922). Age, sex, and acetylization status has no significant correlation with DIH incidence in general. Significant association between slow acetylator phenotype and incidence of moderate DIH was identified (OR=7.125; 95% CI= 1.309-38.711). In conclusion, some risk factors were correlated to DIH incidence in pulmonary TB patientsreceiving standart TB treatment regimen.
Pengelompokan Genotip, Serologi dan Supertipe Gene HLA Kelas I pada Suku Jawa, Indonesia Yuliwulandari, Rika; Teguh Rochani, Jekti; Indrawati, Isna
Jurnal Kedokteran YARSI Vol 18, No 2 (2010): MEI - AGUSTUS 2010
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.48 KB) | DOI: 10.33476/jky.v18i2.182

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Gen Human Leukocyte Antigen (HLA) berperan penting dalam sistim pertahanan tubuh manusia. Gen ini juga terkenal sebagai gen yang paling polimorfik dalam struktur genom manusia. Oleh karena itu identifikasi gen HLA sangat penting dilakukan pada tiap populasi termasuk populasi suku Jawa. Pada penelitian ini, telah dilakukan identifikasi genotipe HLA pada 237 subyek. Namun demikian, selain genotip, perlu juga dilakukan pengelompokan berdasarkan jenis serologi dan supertipe HLA. Makalah ini menyajikan pengelompokan gen HLA kelas I berdasarkan database yang umum dirujuk oleh peneliti-peneliti lainnya.
Nasopharyngeal Carcinoma (NPC) Related Human Leukocyte Antigen (HLA) Haplotype Sharing among Southern East Asian Population Yuliwulandari, Rika; Tokunaga, Katsushi
Global Medical & Health Communication (GMHC) Vol 5, No 1 (2017)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v5i1.1989

Abstract

The human leukocyte antigens (HLAs) play important roles in the immune systems to response to various pathogens and disease among individuals. The aim of this study was analyze the HLA allele and haplotype frequencies of Southern East Asian population that show high incidence of nasopharyngeal carcinoma (NPC) to evaluate the shared HLA haplotype contribution to NPC susceptibility among the population and analyses the genetic affinities between the population. We collect information of HLA haplotype from our previous study, other published paper, and HLA database in 19 population during 2005 to 2015. Haplotype frequencies were estimated using the maximum likelihood method based on an expectation maximization algorithm with ARLEQUIN v.2.0 software. We also calculated the genetic distance among 19 Southern East Asians based on HLA allele frequency using modified Cavalli-Sforza (DA) distance method. Then, a phylogenetic tree was constructed using DISPAN software and principal component analysis (PCA) was performed using XLSTAT-PRO software. A33-B58-DR3 haplotype, tightly linked to NPC, was commonly observed in all populations, supporting the high incidence of NPC in the populations. In addition, A2-B46 haplotype also associated with NPC, was also commonly found in several population that may also have a role in the disease development. The conclusion is the HLA haplotype sharing has an important role than the HLA allele sharing. The A33-B58-DR3 haplotype and A2-B46-DR9 haplotype in this study could be related to NPC in the Southern East Asian populations. The observed haplotype needs to be tested in the real patients to confirm the assumptionPENYEBARAN HAPLOTIPE HUMAN LEUKOCYTE ANTIGEN (HLA) TERKAIT NASOPHARYNGEAL CARCINOMA (NPC) PADA POPULASI ASIA TIMUR BAGIAN SELATANHuman leukocyte antigens (HLAs) berperan penting dalam sistem imun untuk merespons berbagai patogen dan penyakit di antara individu yang berbeda. Tujuan penelitian ini menganalisis frekuensi alel dan haplotipe HLA populasi Southern East Asia yang menunjukkan insidensi yang tinggi terhadap nasopharyngeal carcinoma (NPC) untuk mengevaluasi kerentanan NPC bagi individu. Informasi haplotipe HLA dikumpulkan dari studi sebelumnya, publikasi jurnal internasional, dan database HLA pada 19 populasi dalam periode tahun 2005–2015. Frekuensi haplotipe dihitung menggunakan metode maximum likelihood berdasarkan expectation maximization algorithm menggunakan piranti lunak ARLEQUIN v.2.0. Jarak genetik di antara 19 populasi Southern East Asians dihitung menggunakan metode modified Cavalli-Sforza (DA) distance. Kemudian, pohon filogenetik dikonstruksi dengan metode neighbor-joining (NJ) menggunakan piranti lunak DISPAN. Principal component analysis (PCA) dilakukan menggunakan piranti lunak XLSTAT-PRO. Haplotipe A33-B58-DR3 terkait erat dengan NPC yang biasa terlihat di semua populasi mendukung tingginya insidensi penyakit dalam populasi. Selain itu, haplotipe A2-B46 juga terkait dengan NPC yang juga ditemukan pada beberapa populasi sehingga kemungkinan memiliki peran dalam perkembangan penyakit. Pada kasus NPC, haplotipe HLA lebih berperan dibanding dengan alel HLA. Haplotipe A33-B58-DR3 dan haplotipe A2-B46-DR9 yang ditemukan terkait dengan NPC pada populasi Southern East Asia. Haplotipe yang diamati tersebut perlu diuji pada pasien nyata untuk mengonfirmasi simpulan.
The Effect of Storage Duration on Fruit Juices Made With or Without the Addition of Yeast Towards Its Alcohol Content Roswiem, Anna Priangani; Septiani, Triayu; Yuliwulandari, Rika
Indonesian Journal of Halal Research Vol 1, No 2 (2019): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v1i2.5753

Abstract

Fruit juice is a healthy beverage. However, we must be careful in handling or consuming such juice, especially in relation to its storage time. The Prophet of Muhammed PBUH in his hadith prohibits to drink fruit juices that have been stored for more than 3 days, as its legal status changes from Halal into Haram.In accordance with the development of science and technology, many methods could be used to measure alcohol content in a sample; such as Gas Chromatography-Mass Spectroscopy method (GC-MS). Therefore the objective of this research was to analyze the alcohol content of various fruit juices made with or without the addition of yeast and  being stored within 1, 2, and 3 days using GC-MS, as well as its relation to the hadith Matn of the Prophet Muhammad PBUH regarding its law. Results showed that alcohol (ethanol) content of various fruit juices : red grapes, green grapes, dates, pomegranate and durian, made without the addition of yeast which stored for 3 days, in consecutive order were 0.524; 0.144; 0.214; 0.143 and 0.314% v/v. While alcohol (ethanol) content of various fruit juices : red grapes, green grapes, dates, pomegranate and durian, made with the addition of 10 g/L yeast were  and stored for 3 days were 0.618; 0.921; 0.901; 0.575 and 1.202% v/v constitutively. Alcohol content of red grapes fruit juices of 0.524% v/v was the highest alcohol content (in juices) which is still Halal to be consumed.