Dita Ayu Mayasari, Dita Ayu
Bachelor of Biomedical Engineering Study Program, Faculty of Science & Technology, Universitas Airlangga, Surabaya

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The Utilization of Achatina fulica Mucus in Alginate Membrane as Wound Healing Accelerator and Anti- Infection Material Rahmawati, Fatkhunisa; Mayasari, Dita Ayu; Adhitioso, Satrio; Putra, Alfian Pramudita; Kuntjoro, Eko Budi; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

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Abstract

Wound should be covered with bandage that is called wound dressing. Most people use synthetic materials such as gauze dressing. Gauze has high absorption of NaCl, which is often used to cleanse the wound. However, discomfort and pain arise since the gauze becomes sticky on the wound. Therefore, we need other alternatives instead of gauze to cover wound. One such alternative is the alginate membrane. This study used alginate membrane with mixture of mucous of the snail Achatina fulica, which contain proteins such as proline, serine asparagine, glycosaminoglycan, hydroxylysine, trionin and so forth, to activate the growth factor. Alginatepowder and carboxymethl cellulose (CMC) was dissolved in distilled water mixed with mucus of the snail Achatina fulica in four variations (4:0; 4:1, 4:2, 4:3) through a magnetic stirrer, and casted on a baking sheet covered with sterile gauze. High Performance Liquid Chromatography (HPLC) test showed that the glycosaminoglycan content was found on the mucous of Achatina fulica. This was indicated by the appearance of peak at 325–350 second. The most optimum alginate and mucus composition was in ratio of 4:2. This ratio resulted in a wound dressing that was still able to absorb the exudate and optimally accelerated wound healing.
The Utilization of Achatina fulica Mucus in Alginate Membrane as Wound Healing Accelerator and Anti- Infection Material Rahmawati, Fatkhunisa; Mayasari, Dita Ayu; Adhitioso, Satrio; Putra, Alfian Pramudita; Kuntjoro, Eko Budi; Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.895 KB) | DOI: 10.20473/ijtid.v5i1.209

Abstract

Wound should be covered with bandage that is called wound dressing. Most people use synthetic materials such as gauze dressing. Gauze has high absorption of NaCl, which is often used to cleanse the wound. However, discomfort and pain arise since the gauze becomes sticky on the wound. Therefore, we need other alternatives instead of gauze to cover wound. One such alternative is the alginate membrane. This study used alginate membrane with mixture of mucous of the snail Achatina fulica, which contain proteins such as proline, serine asparagine, glycosaminoglycan, hydroxylysine, trionin and so forth, to activate the growth factor. Alginatepowder and carboxymethl cellulose (CMC) was dissolved in distilled water mixed with mucus of the snail Achatina fulica in four variations (4:0; 4:1, 4:2, 4:3) through a magnetic stirrer, and casted on a baking sheet covered with sterile gauze. High Performance Liquid Chromatography (HPLC) test showed that the glycosaminoglycan content was found on the mucous of Achatina fulica. This was indicated by the appearance of peak at 325–350 second. The most optimum alginate and mucus composition was in ratio of 4:2. This ratio resulted in a wound dressing that was still able to absorb the exudate and optimally accelerated wound healing.
Evaluation of surface modified PLLA-collagen hollow fiber as vascular graft Mayasari, Dita Ayu; Widiyanti, Prihartini; Rudyardjo, Djony Izak
MEDISAINS Vol 17, No 2 (2019)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v17i2.5259

Abstract

Background: Cardiovascular disease as the first silent killer in the world is related to atherosclerosis plaque. Coronary artery bypass grafting as a gold standard for severe atherosclerosis needs alternative materials to replace the narrowed artery due to plaque. PLLA is one of the biodegradable polymers which is suitable for the vascular graft, but some of its weaknesses should be addressed.Objective: The aim of this research is to improve PLLA characteristics for a vascular graft candidate by using collagen and chitosan.Methods: This study was an experimental study that consisted of two steps, preparation of hollow fiber and evaluation of its characteristic. This research evaluated the effect of chitosan concentration against cytotoxicity and hemocompatibility using MTT assay and hemolytic test.Results: The result showed that chitosan could improve the biological characteristics of hollow fiber PLLA-collagen, non-toxic, and non-hemolytic.Conclusions: There is a good effect on the biological characteristics of PLLA-Collagen hollow fiber by adding chitosan on the surface of PLLA-Collagen hollow fiber.