Saka Winias, Saka
Faculty of Dentistry Airlangga University Surabaya

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EFFECT OF CYNAMMYLDEHYDE FROM CINNAMON EXTRACT AS A NATURAL PRESERVATIVE ALTERNATIVE TO THE GROWTH OF Staphylococcus aureus BACTERIA Winias, Saka
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (631.678 KB)

Abstract

Food is one of the best media for the microorganism to live and grow. Therefore, food is often broken because it has been contaminated by the microorganism. In industry country, approximately 30% of population infected by food borne disease. Food borne disease is caused of phatogen bacteria food borne. Staphylococus aureus is a kind of bacteria that can make food rotten and also it is a phatogen bacteria cause food born disease, no forming spora, positive gram bacteria and the food substance which is contaminated by Staphylococus aureus will cause poisoned becaused of enterotoxin which is heat resisting. Essential oil is antimicrobial and anti bacterial that the most effective, it can inhibit the growing of microba and bacteria. One of the example of essential oil is Cinnamon.sp oil. Cinnamon oil is antimcroba agent for bacteri and fungi because it contain cynammyldehyde and cynammyl alcohol and also eugenol. The aim of this study is to understand the antimcrobacterial potential of cynammyldehyde from cinnamon extract to Staphylococus aureus. This study is laboratory experimantal research. Essential oil from Cinnamon by destilation, then redistilation was done to get cynammyldehyde from cinnamon. Cynammyldehyde was tested to Staphylococus aureus. Test method was done as dilution in the form. From this result, it show that cynammyldehide from cinnamon extract has ability in inhibit the Staphylococus aureus growth. We can conclude that Cynammaldehyde from cinnamon extract has antibacterial effect especially for positive gram bacteria that is Staphylococcus aureus. The optimum inhibiting effort is 0.09%.
EFFECT OF CYNAMMYLDEHYDE FROM CINNAMON EXTRACT AS A NATURAL PRESERVATIVE ALTERNATIVE TO THE GROWTH OF Staphylococcus aureus BACTERIA Winias, Saka
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (631.678 KB) | DOI: 10.20473/ijtid.v2i1.188

Abstract

Food is one of the best media for the microorganism to live and grow. Therefore, food is often broken because it has been contaminated by the microorganism. In industry country, approximately 30% of population infected by food borne disease. Food borne disease is caused of phatogen bacteria food borne. Staphylococus aureus is a kind of bacteria that can make food rotten and also it is a phatogen bacteria cause food born disease, no forming spora, positive gram bacteria and the food substance which is contaminated by Staphylococus aureus will cause poisoned becaused of enterotoxin which is heat resisting. Essential oil is antimicrobial and anti bacterial that the most effective, it can inhibit the growing of microba and bacteria. One of the example of essential oil is Cinnamon.sp oil. Cinnamon oil is antimcroba agent for bacteri and fungi because it contain cynammyldehyde and cynammyl alcohol and also eugenol. The aim of this study is to understand the antimcrobacterial potential of cynammyldehyde from cinnamon extract to Staphylococus aureus. This study is laboratory experimantal research. Essential oil from Cinnamon by destilation, then redistilation was done to get cynammyldehyde from cinnamon. Cynammyldehyde was tested to Staphylococus aureus. Test method was done as dilution in the form. From this result, it show that cynammyldehide from cinnamon extract has ability in inhibit the Staphylococus aureus growth. We can conclude that Cynammaldehyde from cinnamon extract has antibacterial effect especially for positive gram bacteria that is Staphylococcus aureus. The optimum inhibiting effort is 0.09%.
Scaffold combination of chitosan and collagen synthesized from chicken feet induces osteoblast and osteoprotegerin expression in bone healing process of mice Winias, Saka; Ernawati, Diah Savitri; Ariani, Maretaningtias Dwi; Rahayu, Retno Pudji
Dental Journal (Majalah Kedokteran Gigi) Vol 50, No 2 (2017): (June 2017)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.092 KB) | DOI: 10.20473/j.djmkg.v50.i2.p86-90

Abstract

Background: Over 500.000 of the 2,3 million surgical treatments requiring bone grafting procedures that are performed annually are likely to be necessitated by or will result in bone defects that will not regenerate. Treatment to regenerate new tissues is needed, especially for hard tissue repair, which not only relies on a natural osseointegration process, but also requires a physical support to guide the differentiation and proliferation of cells into the targeted functional tissue. Chitosan and collagen extracted from chicken feet combinations are expected to enhance the bioactive surface and provide mechanical strength as a bone graft scaffold. Purpose: The aim of this study was to investigate the role of chitosan and collagen scaffold synthesized from chicken feet applications to increase the expression of Osteoprotegerin (OPG) and osteoblast cells on the fourteenth day of bone healing. Methods: Eighteen three-month old, adult, male, Rattus novergicu strain rodents with a body weight ranging from 200-350 g were kept under controlled environmental conditions. The mice were randomly divided into three groups consisting of three subjects, each treated with collagen, chitosan, chitosancollagen combination (50:50) scaffolds. On the 14th post-treatment day, three members of each group were sacrificed. Examination of Osteoprotegerin (OPG) expression was conducted by means of immunohistochemistry staining with anti-OPG polyclonal antibodies. Meanwhile, osteoblast cell examination was performed by means of hematoxilin-eosin (HE) staining. Results: The mice treated with collagen and a chitosan-collagen combination scaffold presented an increase in the expression of Osteoprotegerin (OPG) and the number of osteoblast cells respectively. Conclusion: A combination of chitosan-collagen (50:50) scaffold extracted from chicken feet increased the expression of OPG and the number of osteoblasts in the bone healing process. The combination scaffolds demonstrated the highest OPG expression and number of osteoblasts compared to the other groups.
RECCURENT TRAUMA-INDUCED APHTHOUS STOMATITIS IN ADJUSTMENT DISORDER PATIENTS Yuliana, Y.; Winias, Saka; Hendarti, Hening Tuti; Soebadi, Bagus
Dental Journal (Majalah Kedokteran Gigi) Vol 52, No 3 (2019): (September 2019)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i3.p163-167

Abstract

Background: Adjustment disorder is a temporary psychological condition related to emotional responses or behaviour in reaction to stress resulting from certain changes in a specific period of an individual?s life yet which does not significantly affect his/her daily life. Recurrent aphthous stomatitis (RAS) constitutes recurrent inflammation of the oral mucosa, in the form of an ulcer, frequently associated with psychological stress. Occasionally, a patient does not realise that she/he is suffering from a psychological disorder until the emergence of clinical symptoms, among them recurrent ulcers the causes of which are unknown. Purpose: This article presents a case of adjustment disorder diagnosed from symptoms observable in the oral cavity. Case: The case involved a 21-year-old student who presented with the symptoms of large, painful ulcers on her tongue, the inside of her cheek, and the floor of her mouth. These symptoms had been observable for one month but remained untreated. The patient only ate once a day or even once every two days. An introverted personality, she did not associate with other people. Case management: The procedure covered anamnesis, clinical examination, blood laboratory tests, total immunoglobulin E (IgE), an Antinuclear Antibody (ANA) test, bacterial and fungal culture in ulcus, questionnaire screening on psychological disorders, cortisol level examination and referral to a psychiatrist. Symptomatic therapy administered to the patient led to recovery of the ulcer in 29 days. Conclusion: RAS can be triggered by psychological stress which induces changes in the immune system and oral mucosa tissue.
HYPERPLASIA OF WISTAR RAT TONGUE MUCOSA DUE TO EXPOSURE TO CIGARETTE SIDE-STREAM SMOKE Ayuningtyas, Nurina Febriyanti; Mahardika, Grahania Octaviono; Soebadi, Bagus; Permadiati, Adiastuti Endah; Winias, Saka; Hendarti, Hening Tuti; Zain, Rosnah Binti
Dental Journal (Majalah Kedokteran Gigi) Vol 52, No 3 (2019): (September 2019)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i3.p133-137

Abstract

Background: Hyperplasia, a condition whereby an excessive number of cells are produced due to their uncontrolled division, represents a common symptom of carcinogenesis. Cancer is a physical manifestation of cell malignancy resulting from abnormal proliferation. Globally, oral cancer currently constitutes the sixth largest lethal form of the condition. The most common etiology of oral cancer is tobacco of which cigarettes are the most popular related product. The health risks associated with cigarette smoke not only affect active smokers but also individuals who ingest it passively. Sidestream smoke comes from the lighted end of  a burning tobacco product such as a cigarette, pipe or cigar and contains nicotine and many harmful cancer-causing chemicals. Inhaling sidestream smoke increases the risk of lung and other types of cancer. Purpose: The purpose of this study was to understand how sidestream cigarette smoke initiates precancerous changes, in this case hyperplasia, in the oral mucosa epithelium of Wistar rats. Methods: The subjects were divided into three groups, a 4-week treatment group (P1), an 8-week treatment group (P2), and a control group (K), each consisting of ten subjects. The subjects were exposed to a daily two-cigarette dose of smoke. The experiment used a post-test only control group design. All samples were sacrificed during the fourth and eighth weeks. Haematoxylin-eosin staining was performed on the tongues of the Wistar rats to establish the presence of hyperplasia. Data was analyzed using a one-way ANOVA test. Results: After the Wistar rats had been exposed to cigarette smoke, an increased degree of epithelial cell proliferation (hyperplasia) showed a significant difference with a p-value <0.05 during the eighth week. Conclusion: Exposure to cigarette sidestream smoke induces increased epithelial cell proliferation (hyperplasia) in Wistar rats.